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The map label for this gene is hpkR [H]

Identifier: 15602268

GI number: 15602268

Start: 471237

End: 472106

Strand: Reverse

Name: hpkR [H]

Synonym: PM0403

Alternate gene names: 15602268

Gene position: 472106-471237 (Counterclockwise)

Preceding gene: 15602270

Following gene: 15602267

Centisome position: 20.91

GC content: 42.64

Gene sequence:

>870_bases
ATGTATAATTTGGAACAACTCAAAGCTTTTGTCGCAGTATGTGAAAATGGCTCGATATCAGCAGCCGCCCGCAAGTTAGG
TAAGGCACAATCAGCAGTTAGTAGTGCTGTGGCAAATTTAGAAATCGCGCTCAATCAAACCTTGTTTGATCGTAGCAAAA
ACGCCGCCCATCTTACGTCTAATGGCGCTGCTTTACTGCCTATGGTAAAACATGTCTTACAGCAAATACACTATTTCGAA
CAAAAAGCGACCGCTCTTTCTGAACCTCAGGAATATCGGTTGAGTATTGCTTTTGAAGACACTTTGTGGGATGAACAACT
CACAAAGATCTTTGTTAAATTGCACCAACGCTTTCCACATACCCAGTTGGATTTACAACTTTCCACCGCGGCTGAAATTC
GCTCAAAAATAGAACAAGGTGAACTGAATATCGGTATCCTCTACCAAAATCAAGTACAATCTGATCTTCCTGTGCAGTTG
CTCGGTCAACAGCGTTTTGTTGCTGCAGCGGCCCCCTCTCACCCATTATCTCAACTTACTGAGGTAAACAATGCCGATTT
AGCACACTTCGTCCAGCTCATCCACCGCAACGCCGACATTACACCGATTTCTCCACAATATTGGCAAATCAATAGTTATT
ACTTGATGATGGACTTAGTGAATCAAGGGATCGGGTGGGCTATTATGCCAGAGCAGCTCTTAGTAGATGAGTTTCTTGGC
TCACAACTTAACAGGTTACCATTAACTGCGTTACTCCACCCCAAACCTCTAGACATTACTGCTATGACTGCATTTAATCA
TTATCATGGCAAAGTTACGCAATTTGTACTGGCAGAACTGAAAGAGGTATTTCATACGCAAAGAAGTTAA

Upstream 100 bases:

>100_bases
ATTGTAAAAAGAAAATATTGCTATGAAAAATCAATGGCTATCTATTTTTCAGATAGCAAAGATTAAATTCAAACGATAAA
ATAGATAAAAAGGAGGAGAT

Downstream 100 bases:

>100_bases
AAATATCCAAACCAAATATAACAATGAGGAGGAGGTTGTGAGGGCAAACAAAGAAGGCGTAGAAAATGTTGTTATTAACG
TAAACTCATTACTACCTTGC

Product: HpkR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MYNLEQLKAFVAVCENGSISAAARKLGKAQSAVSSAVANLEIALNQTLFDRSKNAAHLTSNGAALLPMVKHVLQQIHYFE
QKATALSEPQEYRLSIAFEDTLWDEQLTKIFVKLHQRFPHTQLDLQLSTAAEIRSKIEQGELNIGILYQNQVQSDLPVQL
LGQQRFVAAAAPSHPLSQLTEVNNADLAHFVQLIHRNADITPISPQYWQINSYYLMMDLVNQGIGWAIMPEQLLVDEFLG
SQLNRLPLTALLHPKPLDITAMTAFNHYHGKVTQFVLAELKEVFHTQRS

Sequences:

>Translated_289_residues
MYNLEQLKAFVAVCENGSISAAARKLGKAQSAVSSAVANLEIALNQTLFDRSKNAAHLTSNGAALLPMVKHVLQQIHYFE
QKATALSEPQEYRLSIAFEDTLWDEQLTKIFVKLHQRFPHTQLDLQLSTAAEIRSKIEQGELNIGILYQNQVQSDLPVQL
LGQQRFVAAAAPSHPLSQLTEVNNADLAHFVQLIHRNADITPISPQYWQINSYYLMMDLVNQGIGWAIMPEQLLVDEFLG
SQLNRLPLTALLHPKPLDITAMTAFNHYHGKVTQFVLAELKEVFHTQRS
>Mature_289_residues
MYNLEQLKAFVAVCENGSISAAARKLGKAQSAVSSAVANLEIALNQTLFDRSKNAAHLTSNGAALLPMVKHVLQQIHYFE
QKATALSEPQEYRLSIAFEDTLWDEQLTKIFVKLHQRFPHTQLDLQLSTAAEIRSKIEQGELNIGILYQNQVQSDLPVQL
LGQQRFVAAAAPSHPLSQLTEVNNADLAHFVQLIHRNADITPISPQYWQINSYYLMMDLVNQGIGWAIMPEQLLVDEFLG
SQLNRLPLTALLHPKPLDITAMTAFNHYHGKVTQFVLAELKEVFHTQRS

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=241, Percent_Identity=28.2157676348548, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082132, Length=279, Percent_Identity=23.6559139784946, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786713, Length=315, Percent_Identity=23.1746031746032, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082024, Length=176, Percent_Identity=31.8181818181818, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786508, Length=262, Percent_Identity=23.2824427480916, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788887, Length=241, Percent_Identity=25.3112033195021, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787601, Length=265, Percent_Identity=21.5094339622642, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32461; Mature: 32461

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNLEQLKAFVAVCENGSISAAARKLGKAQSAVSSAVANLEIALNQTLFDRSKNAAHLTS
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
NGAALLPMVKHVLQQIHYFEQKATALSEPQEYRLSIAFEDTLWDEQLTKIFVKLHQRFPH
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEEHHHHHHHHHHHHHHHHHHCCC
TQLDLQLSTAAEIRSKIEQGELNIGILYQNQVQSDLPVQLLGQQRFVAAAAPSHPLSQLT
CEEEEEHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHCCHHEEEECCCCCHHHHHH
EVNNADLAHFVQLIHRNADITPISPQYWQINSYYLMMDLVNQGIGWAIMPEQLLVDEFLG
HCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCEEHHHHHHCCCCEEECHHHHHHHHHHH
SQLNRLPLTALLHPKPLDITAMTAFNHYHGKVTQFVLAELKEVFHTQRS
HHHHCCCCCEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYNLEQLKAFVAVCENGSISAAARKLGKAQSAVSSAVANLEIALNQTLFDRSKNAAHLTS
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
NGAALLPMVKHVLQQIHYFEQKATALSEPQEYRLSIAFEDTLWDEQLTKIFVKLHQRFPH
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEEHHHHHHHHHHHHHHHHHHCCC
TQLDLQLSTAAEIRSKIEQGELNIGILYQNQVQSDLPVQLLGQQRFVAAAAPSHPLSQLT
CEEEEEHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHCCHHEEEECCCCCHHHHHH
EVNNADLAHFVQLIHRNADITPISPQYWQINSYYLMMDLVNQGIGWAIMPEQLLVDEFLG
HCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCEEHHHHHHCCCCEEECHHHHHHHHHHH
SQLNRLPLTALLHPKPLDITAMTAFNHYHGKVTQFVLAELKEVFHTQRS
HHHHCCCCCEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]