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The map label for this gene is msbB [H]

Identifier: 15602227

GI number: 15602227

Start: 423825

End: 424781

Strand: Direct

Name: msbB [H]

Synonym: PM0362

Alternate gene names: 15602227

Gene position: 423825-424781 (Clockwise)

Preceding gene: 15602226

Following gene: 15602228

Centisome position: 18.77

GC content: 41.38

Gene sequence:

>957_bases
ATGGCAATGTCAGAAGAAGATACACGTTTAACGGCTCGCAGTGGTTATCAACCACAGTTTTCATGGTCCTATTTACAGCC
AAAATATTGGTTAATTTGGTTAGGCATTTTCGGCTTGATTTTACTTGCCTTTGTGCCGTTTCGTTTACGTGATAAGTTGG
CCAGTTACATTGGGAAAATTGTGGCAAGGAAAGCCAAAAAGCAACGTCATCGTGCCAAGATTAATTTACAATATTGTTTT
CCTCACTGGACAGAAGCGCAACGTGAACAGACAATCGAAGAAATGTTTGTGATTGTGACGCAAGTCATGTTAGGAATTGG
GGAAATTGCGATACGCTCGAAACGACATTTACAACGCCGTAGTTGTTTCACCGGGCTAGAACATATTCATCGTGCTCGTG
AGCAGGGCAAAAATATTATTCTATTAGTGCCTCATGCTTGGGCAATCGATGCCTCAGGGATTATTTTACATACCCATGGT
ATGCCAATGACTTCTATGTATAATCCACACCGTAATCCCTTGGTTGATTGGTTATGGACTTTTGCCCGTCAACGTTTTGG
TGGCAAAATGCATGCCCGTCAAAATGGGATTAAACCGTTTTTAAATCATGTGAAGCAAGGTGATATGGGCTATTATTTAC
CAGATGAAGATTATGGGGCAGAGTTAAGTGTCTTTGTGGATTTTTTTGCCACATACAAAGCCACTTTACCGGGCATTAAT
AAAATTGCACGCTTAGCGAAAGCAGCGGTGATCCCGATGTTTCCTCGCTATAATGCCTTATCGGGAAAATATGAAATAGA
GATTCATCCTGCTATGACATTGAGTGATGATCCTGAACAAGCAGCTCGCGCGATGAATCAAGAAATTGAAAGTTTTGTGA
CGGCTACACCGGCACAATATGTTTGGATTTTACGTTTGCTAAAAACCCGTAAAGACGGCACCGACATTTATGCTTAA

Upstream 100 bases:

>100_bases
TCAGTAAAGGGAAAACATTAATTCGCCCAATGGTCGTGGTGATGTCATTTATCATGACAATGAAAATGGCCTATGATCAA
GGTTGGTTTAGCTAATAGGA

Downstream 100 bases:

>100_bases
GTCAGTGGGCATGTCTGCTTGTCAACGTGATGATCGTCTCTTTTTATCATGATATGACAGCGCTGAACGGGTTGATAGAA
AAGGAGTAAGCCTCGTAGAA

Product: lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MAMSEEDTRLTARSGYQPQFSWSYLQPKYWLIWLGIFGLILLAFVPFRLRDKLASYIGKIVARKAKKQRHRAKINLQYCF
PHWTEAQREQTIEEMFVIVTQVMLGIGEIAIRSKRHLQRRSCFTGLEHIHRAREQGKNIILLVPHAWAIDASGIILHTHG
MPMTSMYNPHRNPLVDWLWTFARQRFGGKMHARQNGIKPFLNHVKQGDMGYYLPDEDYGAELSVFVDFFATYKATLPGIN
KIARLAKAAVIPMFPRYNALSGKYEIEIHPAMTLSDDPEQAARAMNQEIESFVTATPAQYVWILRLLKTRKDGTDIYA

Sequences:

>Translated_318_residues
MAMSEEDTRLTARSGYQPQFSWSYLQPKYWLIWLGIFGLILLAFVPFRLRDKLASYIGKIVARKAKKQRHRAKINLQYCF
PHWTEAQREQTIEEMFVIVTQVMLGIGEIAIRSKRHLQRRSCFTGLEHIHRAREQGKNIILLVPHAWAIDASGIILHTHG
MPMTSMYNPHRNPLVDWLWTFARQRFGGKMHARQNGIKPFLNHVKQGDMGYYLPDEDYGAELSVFVDFFATYKATLPGIN
KIARLAKAAVIPMFPRYNALSGKYEIEIHPAMTLSDDPEQAARAMNQEIESFVTATPAQYVWILRLLKTRKDGTDIYA
>Mature_317_residues
AMSEEDTRLTARSGYQPQFSWSYLQPKYWLIWLGIFGLILLAFVPFRLRDKLASYIGKIVARKAKKQRHRAKINLQYCFP
HWTEAQREQTIEEMFVIVTQVMLGIGEIAIRSKRHLQRRSCFTGLEHIHRAREQGKNIILLVPHAWAIDASGIILHTHGM
PMTSMYNPHRNPLVDWLWTFARQRFGGKMHARQNGIKPFLNHVKQGDMGYYLPDEDYGAELSVFVDFFATYKATLPGINK
IARLAKAAVIPMFPRYNALSGKYEIEIHPAMTLSDDPEQAARAMNQEIESFVTATPAQYVWILRLLKTRKDGTDIYA

Specific function: Transfers myristate or laurate, activated on ACP, to (KDO)2-(lauroyl)-lipid IVA [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1788161, Length=302, Percent_Identity=44.3708609271523, Blast_Score=289, Evalue=1e-79,
Organism=Escherichia coli, GI1787292, Length=302, Percent_Identity=32.4503311258278, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87082094, Length=299, Percent_Identity=30.7692307692308, Blast_Score=144, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011921 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 36769; Mature: 36638

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMSEEDTRLTARSGYQPQFSWSYLQPKYWLIWLGIFGLILLAFVPFRLRDKLASYIGKI
CCCCCCCCEEHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VARKAKKQRHRAKINLQYCFPHWTEAQREQTIEEMFVIVTQVMLGIGEIAIRSKRHLQRR
HHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SCFTGLEHIHRAREQGKNIILLVPHAWAIDASGIILHTHGMPMTSMYNPHRNPLVDWLWT
HHHHHHHHHHHHHHCCCCEEEEECCCEEECCCCEEEEECCCCCHHCCCCCCCHHHHHHHH
FARQRFGGKMHARQNGIKPFLNHVKQGDMGYYLPDEDYGAELSVFVDFFATYKATLPGIN
HHHHHHCCHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHH
KIARLAKAAVIPMFPRYNALSGKYEIEIHPAMTLSDDPEQAARAMNQEIESFVTATPAQY
HHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHH
VWILRLLKTRKDGTDIYA
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AMSEEDTRLTARSGYQPQFSWSYLQPKYWLIWLGIFGLILLAFVPFRLRDKLASYIGKI
CCCCCCCEEHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VARKAKKQRHRAKINLQYCFPHWTEAQREQTIEEMFVIVTQVMLGIGEIAIRSKRHLQRR
HHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SCFTGLEHIHRAREQGKNIILLVPHAWAIDASGIILHTHGMPMTSMYNPHRNPLVDWLWT
HHHHHHHHHHHHHHCCCCEEEEECCCEEECCCCEEEEECCCCCHHCCCCCCCHHHHHHHH
FARQRFGGKMHARQNGIKPFLNHVKQGDMGYYLPDEDYGAELSVFVDFFATYKATLPGIN
HHHHHHCCHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHH
KIARLAKAAVIPMFPRYNALSGKYEIEIHPAMTLSDDPEQAARAMNQEIESFVTATPAQY
HHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHH
VWILRLLKTRKDGTDIYA
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]