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The map label for this gene is mnmC

Identifier: 15602203

GI number: 15602203

Start: 396973

End: 398991

Strand: Reverse

Name: mnmC

Synonym: PM0338

Alternate gene names: 15602203

Gene position: 398991-396973 (Counterclockwise)

Preceding gene: 15602205

Following gene: 15602202

Centisome position: 17.67

GC content: 43.78

Gene sequence:

>2019_bases
ATGCATAAAGTTCAGTTTGCTGATGTCCATTTTAATGCTGAAAACACGCCGGTTTCTGCCCAATTTGACGATGTTTATTT
TTCCAATCAAGACGGTCTTGCGGAAAGTCACTATATTTTTCAGGAAGGTAATCAATTATGGCAACGCTGGCAACAAACAA
GTGAAGCGCATTTTGTGATTGCGGAAACTGGCTTTGGTACAGGGCTGAATTTTTTTGCAGTCACTCAGCGTTTTCGTGAA
TTTCGCCTTACTTATCCTGACGCACCGCTAAAACGGCTCTTTTTTATTTCCTTTGAAAAATACCCTTTACCCTTAGCACA
ACTCAAGCGGGCACATCAAGCCTACCCTGAGTTTCAATCTCTTGCACAACAATTACAACAATCTTGGTTAGAACCGATTG
TCGGTTGCTACCGTTTTCACTTTGCTGAAACCACACTTGATCTCTGGTTTGGTGATATGGCGGAAAACCTGCCTCAGTTA
GGCGATTATATGTGCAATAAAATTGATGCGTGGTTTTTAGACGGTTTTGCCCCGAGTAAAAATCCACAAATGTGGCAAGA
TACCCTTTACCAACACATGTATCGTTACACCAAAACACAAGGCACTTTTTCTACTTTTACCGCAGCAAGTGCCGTAAGAA
AAGGTCTAATACATGCGGGTTTTACAGTCACTAAACGCAAAGGCTATGGCAAAAAACGAGAATGTTTACAGGGTGTAAAA
GCACAACAACAGCCCGCTGACATCCATGCCCCTTGGGCCCTTGTTCAACCGGCTGAACTGACAGAAAATGCAGATATTGC
CCTGATTGGTGGAGGGATTGCCTCCGTATTTAGCGCGCTTTCCTTATTAGAGCGAGGAGCAAAAGTAACTGTATATTGCG
AAGACAACTCACTTGCCGCCAATGCGTCAGGCAATAAACAAGGGGCATTTTATCCCCAATTAAGCGATGATGATTTACGC
TATATTCGCTTCTACATCCATGCCTTTGCCTATGGTAAACAGCGCTTTAACTGGGCAATTGAACAGGGTATTACATTTGA
GCATGATTTTTGTGGCGTCGCACTCTGTGCCTATGATGCCAAAAGTGCGGTCAAATTAGCGAAAATTTCCGCGCTCCAAT
TGCCCCCTAGCCTGTATCAGCCCTTATCACAACAAGCACTTAGCGAACAAGTCGGGCTACCACTACCATGTGATGGTGGC
TTTATCGCACAAGGCGCTTGGTTATCACCACAACAATTTGTTCAAAATACTTTCGATTTTTTAGCAACACGTGGTGTCAT
CATTAAAACACAGCAAAAAATCACCGCACTTGAACGACAAACCACGCATTGGCTCTTAACAACAGAACAAGGCGACAGTT
TCAAACATCAAGTTGTTGTACTGGCAAATGGTCACAAACTCACACAATTTCGTCAAACAGAACATTTACCTGTTTATCCT
GTACGTGGACAAGTGAGTGAAATTGCCACTTCTACTGAACTAAGCAAACTCAAGGCTGTCATTTGCTATGATGGTTATCT
AACCCCAAAAGCAGCGAGTCAAACCCATTGCTTGGGTGCCAGCCATTTGCGTGATAATGCTGAGCGGGCATTTAGCTTAC
AAGAACAGCAAGAAAACCAACAAAAAATCCAAACGAATCTTGCCTCGGTGGATTGGGTCAATGAGGTTGATACCCGCAAT
AATCAAGCCAGAATTGGCATCCGTTGCTCCGTTCGTGATCGTATCCCAATGCTAGGGAATGTGCCTGATTTTGAACAACA
GTTGAGTGATTATCACAATCTATACAACTTACGCCGTCGCAAACACGCCATAAAACACGCCGCACTGCATCCGAATCTCT
TTCTAATTGGTGCTCTCGGTTCTAGAGGACTGACCTCTGCACCGCTGTTAGCAGAAACCCTGGCTTCGCTGATTTATCAC
GAGCCCTTGCCTCTCAGTGAAGATATTCTCCATCAACTGAATGCAAACCGCAGCTGGATACGAAAATTATTAAAAGGAAG
TCAAGTAAAACAGGGCTAA

Upstream 100 bases:

>100_bases
AAACGTTAACATTTCAAGTGCTAATTTGATCGTTCGACCAGTATATAAATTTTCGCTATTATATTACGATTCAGGTTGTC
TATAAAGGAATAAAAAAATC

Downstream 100 bases:

>100_bases
AATGCCCTCTTGCTTTTGCGCCTGCTCCTGTTCTTATTACAATAAAAACGCTTACAAAAATACGCATTTTTTTGTATATA
TCACCTCCATTCTTAGCAAT

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MHKVQFADVHFNAENTPVSAQFDDVYFSNQDGLAESHYIFQEGNQLWQRWQQTSEAHFVIAETGFGTGLNFFAVTQRFRE
FRLTYPDAPLKRLFFISFEKYPLPLAQLKRAHQAYPEFQSLAQQLQQSWLEPIVGCYRFHFAETTLDLWFGDMAENLPQL
GDYMCNKIDAWFLDGFAPSKNPQMWQDTLYQHMYRYTKTQGTFSTFTAASAVRKGLIHAGFTVTKRKGYGKKRECLQGVK
AQQQPADIHAPWALVQPAELTENADIALIGGGIASVFSALSLLERGAKVTVYCEDNSLAANASGNKQGAFYPQLSDDDLR
YIRFYIHAFAYGKQRFNWAIEQGITFEHDFCGVALCAYDAKSAVKLAKISALQLPPSLYQPLSQQALSEQVGLPLPCDGG
FIAQGAWLSPQQFVQNTFDFLATRGVIIKTQQKITALERQTTHWLLTTEQGDSFKHQVVVLANGHKLTQFRQTEHLPVYP
VRGQVSEIATSTELSKLKAVICYDGYLTPKAASQTHCLGASHLRDNAERAFSLQEQQENQQKIQTNLASVDWVNEVDTRN
NQARIGIRCSVRDRIPMLGNVPDFEQQLSDYHNLYNLRRRKHAIKHAALHPNLFLIGALGSRGLTSAPLLAETLASLIYH
EPLPLSEDILHQLNANRSWIRKLLKGSQVKQG

Sequences:

>Translated_672_residues
MHKVQFADVHFNAENTPVSAQFDDVYFSNQDGLAESHYIFQEGNQLWQRWQQTSEAHFVIAETGFGTGLNFFAVTQRFRE
FRLTYPDAPLKRLFFISFEKYPLPLAQLKRAHQAYPEFQSLAQQLQQSWLEPIVGCYRFHFAETTLDLWFGDMAENLPQL
GDYMCNKIDAWFLDGFAPSKNPQMWQDTLYQHMYRYTKTQGTFSTFTAASAVRKGLIHAGFTVTKRKGYGKKRECLQGVK
AQQQPADIHAPWALVQPAELTENADIALIGGGIASVFSALSLLERGAKVTVYCEDNSLAANASGNKQGAFYPQLSDDDLR
YIRFYIHAFAYGKQRFNWAIEQGITFEHDFCGVALCAYDAKSAVKLAKISALQLPPSLYQPLSQQALSEQVGLPLPCDGG
FIAQGAWLSPQQFVQNTFDFLATRGVIIKTQQKITALERQTTHWLLTTEQGDSFKHQVVVLANGHKLTQFRQTEHLPVYP
VRGQVSEIATSTELSKLKAVICYDGYLTPKAASQTHCLGASHLRDNAERAFSLQEQQENQQKIQTNLASVDWVNEVDTRN
NQARIGIRCSVRDRIPMLGNVPDFEQQLSDYHNLYNLRRRKHAIKHAALHPNLFLIGALGSRGLTSAPLLAETLASLIYH
EPLPLSEDILHQLNANRSWIRKLLKGSQVKQG
>Mature_672_residues
MHKVQFADVHFNAENTPVSAQFDDVYFSNQDGLAESHYIFQEGNQLWQRWQQTSEAHFVIAETGFGTGLNFFAVTQRFRE
FRLTYPDAPLKRLFFISFEKYPLPLAQLKRAHQAYPEFQSLAQQLQQSWLEPIVGCYRFHFAETTLDLWFGDMAENLPQL
GDYMCNKIDAWFLDGFAPSKNPQMWQDTLYQHMYRYTKTQGTFSTFTAASAVRKGLIHAGFTVTKRKGYGKKRECLQGVK
AQQQPADIHAPWALVQPAELTENADIALIGGGIASVFSALSLLERGAKVTVYCEDNSLAANASGNKQGAFYPQLSDDDLR
YIRFYIHAFAYGKQRFNWAIEQGITFEHDFCGVALCAYDAKSAVKLAKISALQLPPSLYQPLSQQALSEQVGLPLPCDGG
FIAQGAWLSPQQFVQNTFDFLATRGVIIKTQQKITALERQTTHWLLTTEQGDSFKHQVVVLANGHKLTQFRQTEHLPVYP
VRGQVSEIATSTELSKLKAVICYDGYLTPKAASQTHCLGASHLRDNAERAFSLQEQQENQQKIQTNLASVDWVNEVDTRN
NQARIGIRCSVRDRIPMLGNVPDFEQQLSDYHNLYNLRRRKHAIKHAALHPNLFLIGALGSRGLTSAPLLAETLASLIYH
EPLPLSEDILHQLNANRSWIRKLLKGSQVKQG

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=674, Percent_Identity=46.2908011869436, Blast_Score=591, Evalue=1e-170,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_PASMU (Q9CNT7)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245275.1
- ProteinModelPortal:   Q9CNT7
- SMR:   Q9CNT7
- GeneID:   1243685
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0338
- NMPDR:   fig|272843.1.peg.338
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- ProtClustDB:   PRK01747
- BioCyc:   PMUL272843:PM0338-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 75946; Mature: 75946

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKVQFADVHFNAENTPVSAQFDDVYFSNQDGLAESHYIFQEGNQLWQRWQQTSEAHFVI
CCCEEEEEEEECCCCCCCCCEECCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
AETGFGTGLNFFAVTQRFREFRLTYPDAPLKRLFFISFEKYPLPLAQLKRAHQAYPEFQS
EECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCCCCCHHHHHHHHHCCHHHHH
LAQQLQQSWLEPIVGCYRFHFAETTLDLWFGDMAENLPQLGDYMCNKIDAWFLDGFAPSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NPQMWQDTLYQHMYRYTKTQGTFSTFTAASAVRKGLIHAGFTVTKRKGYGKKRECLQGVK
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCC
AQQQPADIHAPWALVQPAELTENADIALIGGGIASVFSALSLLERGAKVTVYCEDNSLAA
CCCCCCCCCCCEEECCCHHHCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCEEE
NASGNKQGAFYPQLSDDDLRYIRFYIHAFAYGKQRFNWAIEQGITFEHDFCGVALCAYDA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCC
KSAVKLAKISALQLPPSLYQPLSQQALSEQVGLPLPCDGGFIAQGAWLSPQQFVQNTFDF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHH
LATRGVIIKTQQKITALERQTTHWLLTTEQGDSFKHQVVVLANGHKLTQFRQTEHLPVYP
HHHCCEEEECHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCEEHHHHCCCCCCEEE
VRGQVSEIATSTELSKLKAVICYDGYLTPKAASQTHCLGASHLRDNAERAFSLQEQQENQ
CCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QKIQTNLASVDWVNEVDTRNNQARIGIRCSVRDRIPMLGNVPDFEQQLSDYHNLYNLRRR
HHHHHHHHHHCCHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KHAIKHAALHPNLFLIGALGSRGLTSAPLLAETLASLIYHEPLPLSEDILHQLNANRSWI
HHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHH
RKLLKGSQVKQG
HHHHCCCCCCCC
>Mature Secondary Structure
MHKVQFADVHFNAENTPVSAQFDDVYFSNQDGLAESHYIFQEGNQLWQRWQQTSEAHFVI
CCCEEEEEEEECCCCCCCCCEECCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
AETGFGTGLNFFAVTQRFREFRLTYPDAPLKRLFFISFEKYPLPLAQLKRAHQAYPEFQS
EECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCCCCCHHHHHHHHHCCHHHHH
LAQQLQQSWLEPIVGCYRFHFAETTLDLWFGDMAENLPQLGDYMCNKIDAWFLDGFAPSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NPQMWQDTLYQHMYRYTKTQGTFSTFTAASAVRKGLIHAGFTVTKRKGYGKKRECLQGVK
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCC
AQQQPADIHAPWALVQPAELTENADIALIGGGIASVFSALSLLERGAKVTVYCEDNSLAA
CCCCCCCCCCCEEECCCHHHCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCEEE
NASGNKQGAFYPQLSDDDLRYIRFYIHAFAYGKQRFNWAIEQGITFEHDFCGVALCAYDA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCC
KSAVKLAKISALQLPPSLYQPLSQQALSEQVGLPLPCDGGFIAQGAWLSPQQFVQNTFDF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHH
LATRGVIIKTQQKITALERQTTHWLLTTEQGDSFKHQVVVLANGHKLTQFRQTEHLPVYP
HHHCCEEEECHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCEEHHHHCCCCCCEEE
VRGQVSEIATSTELSKLKAVICYDGYLTPKAASQTHCLGASHLRDNAERAFSLQEQQENQ
CCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QKIQTNLASVDWVNEVDTRNNQARIGIRCSVRDRIPMLGNVPDFEQQLSDYHNLYNLRRR
HHHHHHHHHHCCHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KHAIKHAALHPNLFLIGALGSRGLTSAPLLAETLASLIYHEPLPLSEDILHQLNANRSWI
HHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHH
RKLLKGSQVKQG
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100