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The map label for this gene is grpE [H]

Identifier: 15602199

GI number: 15602199

Start: 388330

End: 388935

Strand: Direct

Name: grpE [H]

Synonym: PM0334

Alternate gene names: 15602199

Gene position: 388330-388935 (Clockwise)

Preceding gene: 15602196

Following gene: 15602200

Centisome position: 17.2

GC content: 40.43

Gene sequence:

>606_bases
ATGGAGAACGAGATGTCAAACAAAGAGCAACACATTGAAAAAGAAGAGCAACTTCAAGAAGAAAAACATGAGGAACAACA
AAAAACCGAAGAAACAGAAGTTGAAGCTGTGAATGGGGTTGATCCTCTTGAAGAAGCCATTTTGCGGGTACAAGAATTAG
AAGCACAGCTGACAGAAATGGTCAAAAAAGAACAGGATTTTTTACTGCGTAGTCGTGCTGAAATGGATAATATCCGCCGT
CGTGCTGAGCAAGATGTGGAGAAAGCCCATAAGTTTGGATTAGAGAAATTCTCAAAAGATATTTTAAATACCATTGATAA
TTTAGAGCGTGCTTTAGCGACGCCAGCAAACTTAGAAGATGAAAGTATTAAGTCATTGTTCGATGGCGTCGAATTGACTT
TAAAAGAATTACTCGCCACGGTTTCTCGCTTTGGGGTAGAGGCGGTTGGTGTAGTTGGTGAAACCTTCAACCCAGAAGTT
CATCAAGCGATTTCGATGCAACCAATGGAAGGTTTTGAAACCAACCAAATTACAGTTGTTTTACAAAAAGGGTATTTATT
AAATGGACGAGTGATCCGCCCTGCCATGGTGATGGTTGCCGCTTAA

Upstream 100 bases:

>100_bases
AGTCATTATATTACACACAAATCCTTTTATTTGTCAGCAAGTATCTATAGATAGGCTGACTTTTATATTTTTGTTAGAAT
GAGCCAACAGGTTTACATGA

Downstream 100 bases:

>100_bases
GTTTTTATCCCTCATCGTATGCCCACTGATGAATATTGTTCATCAGTGGGCTTTTTTTATCCATTTTTTCTATTTTATAT
AGCGAAAAGGTATAAATAAT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MENEMSNKEQHIEKEEQLQEEKHEEQQKTEETEVEAVNGVDPLEEAILRVQELEAQLTEMVKKEQDFLLRSRAEMDNIRR
RAEQDVEKAHKFGLEKFSKDILNTIDNLERALATPANLEDESIKSLFDGVELTLKELLATVSRFGVEAVGVVGETFNPEV
HQAISMQPMEGFETNQITVVLQKGYLLNGRVIRPAMVMVAA

Sequences:

>Translated_201_residues
MENEMSNKEQHIEKEEQLQEEKHEEQQKTEETEVEAVNGVDPLEEAILRVQELEAQLTEMVKKEQDFLLRSRAEMDNIRR
RAEQDVEKAHKFGLEKFSKDILNTIDNLERALATPANLEDESIKSLFDGVELTLKELLATVSRFGVEAVGVVGETFNPEV
HQAISMQPMEGFETNQITVVLQKGYLLNGRVIRPAMVMVAA
>Mature_201_residues
MENEMSNKEQHIEKEEQLQEEKHEEQQKTEETEVEAVNGVDPLEEAILRVQELEAQLTEMVKKEQDFLLRSRAEMDNIRR
RAEQDVEKAHKFGLEKFSKDILNTIDNLERALATPANLEDESIKSLFDGVELTLKELLATVSRFGVEAVGVVGETFNPEV
HQAISMQPMEGFETNQITVVLQKGYLLNGRVIRPAMVMVAA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=152, Percent_Identity=34.2105263157895, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI40255109, Length=167, Percent_Identity=32.3353293413174, Blast_Score=81, Evalue=5e-16,
Organism=Escherichia coli, GI1788967, Length=197, Percent_Identity=43.6548223350254, Blast_Score=149, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17552458, Length=143, Percent_Identity=33.5664335664336, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324806, Length=157, Percent_Identity=35.6687898089172, Blast_Score=91, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24653432, Length=187, Percent_Identity=28.8770053475936, Blast_Score=81, Evalue=4e-16,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 22907; Mature: 22907

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENEMSNKEQHIEKEEQLQEEKHEEQQKTEETEVEAVNGVDPLEEAILRVQELEAQLTEM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VKKEQDFLLRSRAEMDNIRRRAEQDVEKAHKFGLEKFSKDILNTIDNLERALATPANLED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ESIKSLFDGVELTLKELLATVSRFGVEAVGVVGETFNPEVHQAISMQPMEGFETNQITVV
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCEEEE
LQKGYLLNGRVIRPAMVMVAA
EECCEEECCCCHHHHHHHHCC
>Mature Secondary Structure
MENEMSNKEQHIEKEEQLQEEKHEEQQKTEETEVEAVNGVDPLEEAILRVQELEAQLTEM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VKKEQDFLLRSRAEMDNIRRRAEQDVEKAHKFGLEKFSKDILNTIDNLERALATPANLED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ESIKSLFDGVELTLKELLATVSRFGVEAVGVVGETFNPEVHQAISMQPMEGFETNQITVV
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCEEEE
LQKGYLLNGRVIRPAMVMVAA
EECCEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]