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The map label for this gene is iscA [H]

Identifier: 15602185

GI number: 15602185

Start: 375980

End: 376303

Strand: Direct

Name: iscA [H]

Synonym: PM0320

Alternate gene names: 15602185

Gene position: 375980-376303 (Clockwise)

Preceding gene: 15602184

Following gene: 15602186

Centisome position: 16.65

GC content: 40.74

Gene sequence:

>324_bases
ATGGGTATTTCCTTAACTGAAACGGCAGCAGATCGGGTACGAACCTTCCTCGCGAATCGTGGTAAAGGCATTGGATTACG
TTTAGGGATCAAAACTTCAGGTTGTTCTGGCTTAGCCTATGTCCTTGAATTTGTAGATGAGTTAAATCCTGAGGATCAGG
TGTTTGAACAACATGGTGTCAAAATCATTGTGGATGCCAAAAGTTTGGTCTATTTAGACGGCACTGAATTAGATTTCGCC
AAAGAAGGATTAAATGAAGGCTTTAAGTTCAATAATCCGAATGTGAAAGATCAATGTGGTTGCGGAGAAAGTTTCCACGT
TTAG

Upstream 100 bases:

>100_bases
AGACTACAAAGCAAAAAAAGAAGCAAAATAATCATCTAAAATTCAGTTGGGGCAATTTTGCCCCTTTCACATTATAAGAC
GCAATGGAGTAGAGAATTTC

Downstream 100 bases:

>100_bases
CTGTTTTTTTATCTGATATTTGAGTGAAAGATGAACCCATTTAATATTTTTGATTTACCAGTTGATTTTCATGTTGATCA
AGCGACATTATCGGCACGTT

Product: hypothetical protein

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 107; Mature: 106

Protein sequence:

>107_residues
MGISLTETAADRVRTFLANRGKGIGLRLGIKTSGCSGLAYVLEFVDELNPEDQVFEQHGVKIIVDAKSLVYLDGTELDFA
KEGLNEGFKFNNPNVKDQCGCGESFHV

Sequences:

>Translated_107_residues
MGISLTETAADRVRTFLANRGKGIGLRLGIKTSGCSGLAYVLEFVDELNPEDQVFEQHGVKIIVDAKSLVYLDGTELDFA
KEGLNEGFKFNNPNVKDQCGCGESFHV
>Mature_106_residues
GISLTETAADRVRTFLANRGKGIGLRLGIKTSGCSGLAYVLEFVDELNPEDQVFEQHGVKIIVDAKSLVYLDGTELDFAK
EGLNEGFKFNNPNVKDQCGCGESFHV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=106, Percent_Identity=36.7924528301887, Blast_Score=98, Evalue=1e-21,
Organism=Homo sapiens, GI160420328, Length=83, Percent_Identity=39.7590361445783, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=76.6355140186916, Blast_Score=176, Evalue=3e-46,
Organism=Escherichia coli, GI1787974, Length=106, Percent_Identity=46.2264150943396, Blast_Score=112, Evalue=4e-27,
Organism=Escherichia coli, GI1786351, Length=110, Percent_Identity=40, Blast_Score=79, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI193209025, Length=106, Percent_Identity=37.7358490566038, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI25146411, Length=106, Percent_Identity=37.7358490566038, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71998452, Length=106, Percent_Identity=33.9622641509434, Blast_Score=62, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323001, Length=105, Percent_Identity=41.9047619047619, Blast_Score=90, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24642263, Length=106, Percent_Identity=40.5660377358491, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24649697, Length=108, Percent_Identity=29.6296296296296, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 11654; Mature: 11523

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGISLTETAADRVRTFLANRGKGIGLRLGIKTSGCSGLAYVLEFVDELNPEDQVFEQHGV
CCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHCCC
KIIVDAKSLVYLDGTELDFAKEGLNEGFKFNNPNVKDQCGCGESFHV
EEEEECCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GISLTETAADRVRTFLANRGKGIGLRLGIKTSGCSGLAYVLEFVDELNPEDQVFEQHGV
CCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHCCC
KIIVDAKSLVYLDGTELDFAKEGLNEGFKFNNPNVKDQCGCGESFHV
EEEEECCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]