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The map label for this gene is rlmL

Identifier: 15602169

GI number: 15602169

Start: 356182

End: 358341

Strand: Direct

Name: rlmL

Synonym: PM0304

Alternate gene names: 15602169

Gene position: 356182-358341 (Clockwise)

Preceding gene: 15602168

Following gene: 15602170

Centisome position: 15.78

GC content: 43.29

Gene sequence:

>2160_bases
ATGAAACAACTTTTTGCCACCACTGCCCGTGGTTTTGAAGAATTATTGAAAGTCGAACTAACCGACCTTGGTGCGATGGA
TTGCCAAATCACACAAGGCGGTGTGCACTTTCATGCCGATGAGGAAACGCAATATCGCGTGTTGTTATGGACACGTTTAG
CCTCGCGGATTTTATTACCCATTGTGACTTGCAAAGTGTATAGCGATTTAGATTTGTATTCTGCGGTGGTGGGGCAAAAT
TGGTTGGATTATTTTGATGAAAAAGCCCATTTTATGGTGGATTTTAATGGCACAAACCGAGAAATTCGTCACACCCAATT
TGGTGCAATGCGAGTCAAAGATGGGATTGTGGATTATTTTGAGCGTGTAGGTAAACCGCGTCCAAATGTAGATAAATCAC
AGCCCGATATCCGTATTCATGCCTATTTAAATCGCGAAGAATTAGTGATCTCCTTAGATTTAAGTGGTGATGCTTTACAT
ATGCGCGGTTATCGCGAAGATACGGGCAAAGCCCCGTTACGTGAAACCTTAGCGGCAGCGATCGTCTTACGTTCAGGCTG
GCAGCTGGGCACACCCTTAATGGATCCAATGTGCGGTTCGGGTACCTTGCTGATTGAAGCAGCACAAATGCAAGCCAACA
TTGCCCCGCAATTACACCGTCTGCATTGGGGCTTTGATTTTTGGAAAGGGCACAATCAACAGGCGTGGGATAAGGTGAAA
GGCGAGGCGATTGAATTAGCGGAGCAAACGTTTAACCAAAATCAAAAAGCGAATTTTTATGGCTGCGATCTTGATCATCG
AGTACTGCAAAAAGCCAAACGCAATGCACAAAATGCTGGTGTGGCACATCTTATTCAATGGCAACAAGGCGATGTGGCGG
CATTAAAAAATCCATTTGTTGAAGCAAAAGGTACGGTAATTTGTAATCCACCTTACGGAGAGCGTTTAGGCACGACACCT
GCTTTAATTGCGTTATATTCTGTGTTTGGACAACGTTTAAAACAACAGTTTGCCGATTGGAATGTATCGATTTTCAGTGG
CGAGCCGGCGTTATTAGACTGCTTGCGTTTACGTTCACATCGCCAATTTAAAGCCAAAAATGGTCCGCTCGACTGCGTGC
AAAAAAATTATCATATTAGTGCCCGTGCAACAGCGAGTGACGAGAATACACAAAACTCGCCAGAAATTGACCGCACTTTA
ACTTCTTCTCAAGTGGCAGTGGATTTTGCCAATCGCTTACAAAAAAATAGCAAAAAAATTGAAAAATGGGCGAAACAACA
AGGCATTAATGCTTATCGCTTATACGATGCGGATTTGCCAGAATATAACTTGGCGGTGGATCGCTATGACGATCATATCG
TGGTACAAGAATATGCTGCACCCAAAAATATCGATGAAAACAAAGCACGTCAGCGCTTATTGGATGCAGTAACCGCCACC
TTGCAGGTCACAGGTGTAGAAACCAACAAACTGATTTTAAAAGTGCGCCAAAAACAAAAAGGCACCAACCAGTATGAAAA
ACTCGCCAACAAAGGTGACTATTTCTATGTGAATGAATATGGCGCCAAATTGTGGGTAAACCTAACGGATTATTTGGATA
CGGGACTTTTCCTCGATCACCGTTTAACCCGCAAAATGTTGGGCGAAATGGCGAAAGGCAAAGATGTCCTAAATTTATTT
GCCTATACAGGATCGGCGACGGTACATATGGCGTTAGGTGGTGCCAAATCCACCACCACCGTGGATATGTCAAACACCTA
TTTAAACTGGGCGGAACAGAATTTATTGCTTAATGATTTGGAAGGTAAACAGCATAAGTTAATCCAAGCAGATTGCTTAC
AATGGTTAGCCCGTTGTGACCGTCAGTTTGATCTGATCTTCGTCGACCCACCGACATTTTCTAATTCAAAACGGATGGAA
GATAGCTGGGATGTGCAACGTGATCACATTAAGTTAATGACCCAGTTAAAACGCATTTTACGCCCAAACGGCACCATTGT
GTTTTCCAATAACAAACGTGGCTTTAAAATGGACGTTGAAGGCTTGGCTGCGCTTGGCTTAAGTGCGGTAGAAATTTCTG
CAAAAACCTTGCCATTAGATTTTGAACGCAATAAGCAAATTCATAATTGTTGGGTGGTGAGGCTGAAAGCCGATCTGTAA

Upstream 100 bases:

>100_bases
GTAAAATTTTTGAATTTTTTACCGCACTTTTCCTTTCCTCCGTTTTTGCAGGTGAAAACCTGTTATAATTTCGCCCAGCT
TGAATAACCAGAGAACATTT

Downstream 100 bases:

>100_bases
GCCAGCGAACAGATAACTTGCCTTTTGTAAAATTAGCGGTACACTGGTTGTACCGCTGATTTTTTAAGTGGCTTAGGAAA
TTGTTTTATTCTATTTTTTT

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 719; Mature: 719

Protein sequence:

>719_residues
MKQLFATTARGFEELLKVELTDLGAMDCQITQGGVHFHADEETQYRVLLWTRLASRILLPIVTCKVYSDLDLYSAVVGQN
WLDYFDEKAHFMVDFNGTNREIRHTQFGAMRVKDGIVDYFERVGKPRPNVDKSQPDIRIHAYLNREELVISLDLSGDALH
MRGYREDTGKAPLRETLAAAIVLRSGWQLGTPLMDPMCGSGTLLIEAAQMQANIAPQLHRLHWGFDFWKGHNQQAWDKVK
GEAIELAEQTFNQNQKANFYGCDLDHRVLQKAKRNAQNAGVAHLIQWQQGDVAALKNPFVEAKGTVICNPPYGERLGTTP
ALIALYSVFGQRLKQQFADWNVSIFSGEPALLDCLRLRSHRQFKAKNGPLDCVQKNYHISARATASDENTQNSPEIDRTL
TSSQVAVDFANRLQKNSKKIEKWAKQQGINAYRLYDADLPEYNLAVDRYDDHIVVQEYAAPKNIDENKARQRLLDAVTAT
LQVTGVETNKLILKVRQKQKGTNQYEKLANKGDYFYVNEYGAKLWVNLTDYLDTGLFLDHRLTRKMLGEMAKGKDVLNLF
AYTGSATVHMALGGAKSTTTVDMSNTYLNWAEQNLLLNDLEGKQHKLIQADCLQWLARCDRQFDLIFVDPPTFSNSKRME
DSWDVQRDHIKLMTQLKRILRPNGTIVFSNNKRGFKMDVEGLAALGLSAVEISAKTLPLDFERNKQIHNCWVVRLKADL

Sequences:

>Translated_719_residues
MKQLFATTARGFEELLKVELTDLGAMDCQITQGGVHFHADEETQYRVLLWTRLASRILLPIVTCKVYSDLDLYSAVVGQN
WLDYFDEKAHFMVDFNGTNREIRHTQFGAMRVKDGIVDYFERVGKPRPNVDKSQPDIRIHAYLNREELVISLDLSGDALH
MRGYREDTGKAPLRETLAAAIVLRSGWQLGTPLMDPMCGSGTLLIEAAQMQANIAPQLHRLHWGFDFWKGHNQQAWDKVK
GEAIELAEQTFNQNQKANFYGCDLDHRVLQKAKRNAQNAGVAHLIQWQQGDVAALKNPFVEAKGTVICNPPYGERLGTTP
ALIALYSVFGQRLKQQFADWNVSIFSGEPALLDCLRLRSHRQFKAKNGPLDCVQKNYHISARATASDENTQNSPEIDRTL
TSSQVAVDFANRLQKNSKKIEKWAKQQGINAYRLYDADLPEYNLAVDRYDDHIVVQEYAAPKNIDENKARQRLLDAVTAT
LQVTGVETNKLILKVRQKQKGTNQYEKLANKGDYFYVNEYGAKLWVNLTDYLDTGLFLDHRLTRKMLGEMAKGKDVLNLF
AYTGSATVHMALGGAKSTTTVDMSNTYLNWAEQNLLLNDLEGKQHKLIQADCLQWLARCDRQFDLIFVDPPTFSNSKRME
DSWDVQRDHIKLMTQLKRILRPNGTIVFSNNKRGFKMDVEGLAALGLSAVEISAKTLPLDFERNKQIHNCWVVRLKADL
>Mature_719_residues
MKQLFATTARGFEELLKVELTDLGAMDCQITQGGVHFHADEETQYRVLLWTRLASRILLPIVTCKVYSDLDLYSAVVGQN
WLDYFDEKAHFMVDFNGTNREIRHTQFGAMRVKDGIVDYFERVGKPRPNVDKSQPDIRIHAYLNREELVISLDLSGDALH
MRGYREDTGKAPLRETLAAAIVLRSGWQLGTPLMDPMCGSGTLLIEAAQMQANIAPQLHRLHWGFDFWKGHNQQAWDKVK
GEAIELAEQTFNQNQKANFYGCDLDHRVLQKAKRNAQNAGVAHLIQWQQGDVAALKNPFVEAKGTVICNPPYGERLGTTP
ALIALYSVFGQRLKQQFADWNVSIFSGEPALLDCLRLRSHRQFKAKNGPLDCVQKNYHISARATASDENTQNSPEIDRTL
TSSQVAVDFANRLQKNSKKIEKWAKQQGINAYRLYDADLPEYNLAVDRYDDHIVVQEYAAPKNIDENKARQRLLDAVTAT
LQVTGVETNKLILKVRQKQKGTNQYEKLANKGDYFYVNEYGAKLWVNLTDYLDTGLFLDHRLTRKMLGEMAKGKDVLNLF
AYTGSATVHMALGGAKSTTTVDMSNTYLNWAEQNLLLNDLEGKQHKLIQADCLQWLARCDRQFDLIFVDPPTFSNSKRME
DSWDVQRDHIKLMTQLKRILRPNGTIVFSNNKRGFKMDVEGLAALGLSAVEISAKTLPLDFERNKQIHNCWVVRLKADL

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=714, Percent_Identity=61.2044817927171, Blast_Score=901, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=237, Percent_Identity=29.1139240506329, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_PASMU (Q9CNW9)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245241.1
- ProteinModelPortal:   Q9CNW9
- SMR:   Q9CNW9
- GeneID:   1243651
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0304
- NMPDR:   fig|272843.1.peg.304
- HOGENOM:   HBG289536
- OMA:   YRIYDAD
- ProtClustDB:   PRK11783
- BioCyc:   PMUL272843:PM0304-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114
- PIRSF:   PIRSF037618
- SMART:   SM00981

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 81747; Mature: 81747

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS51165 THUMP; PS01261 UPF0020; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLFATTARGFEELLKVELTDLGAMDCQITQGGVHFHADEETQYRVLLWTRLASRILLP
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCCCEEEHHHHHHHHHHHHH
IVTCKVYSDLDLYSAVVGQNWLDYFDEKAHFMVDFNGTNREIRHTQFGAMRVKDGIVDYF
HHHHHHHCCHHHHHHHHCCHHHHHHCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHH
ERVGKPRPNVDKSQPDIRIHAYLNREELVISLDLSGDALHMRGYREDTGKAPLRETLAAA
HHHCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCEEEECCCCCCCCCCHHHHHHHHH
IVLRSGWQLGTPLMDPMCGSGTLLIEAAQMQANIAPQLHRLHWGFDFWKGHNQQAWDKVK
HHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHCCCCHHHHHHHCCHHHHCCCCCHHHHHHC
GEAIELAEQTFNQNQKANFYGCDLDHRVLQKAKRNAQNAGVAHLIQWQQGDVAALKNPFV
CHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHCCCCE
EAKGTVICNPPYGERLGTTPALIALYSVFGQRLKQQFADWNVSIFSGEPALLDCLRLRSH
ECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHC
RQFKAKNGPLDCVQKNYHISARATASDENTQNSPEIDRTLTSSQVAVDFANRLQKNSKKI
CCCCCCCCCHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHH
EKWAKQQGINAYRLYDADLPEYNLAVDRYDDHIVVQEYAAPKNIDENKARQRLLDAVTAT
HHHHHHCCCCEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHE
LQVTGVETNKLILKVRQKQKGTNQYEKLANKGDYFYVNEYGAKLWVNLTDYLDTGLFLDH
EEEECCCCCHHHEEEHHHHCCCHHHHHHHCCCCEEEEEECCCEEEEEEHHHHCCCHHHHH
RLTRKMLGEMAKGKDVLNLFAYTGSATVHMALGGAKSTTTVDMSNTYLNWAEQNLLLNDL
HHHHHHHHHHHCCCHHEEEEEECCCCEEEEEECCCCCCEEEECCCCCHHHHHCCCEEECC
EGKQHKLIQADCLQWLARCDRQFDLIFVDPPTFSNSKRMEDSWDVQRDHIKLMTQLKRIL
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RPNGTIVFSNNKRGFKMDVEGLAALGLSAVEISAKTLPLDFERNKQIHNCWVVRLKADL
CCCCEEEECCCCCCEEECHHHHHHHCCCEEEEECEECCCCCCCCCCEEEEEEEEEEECC
>Mature Secondary Structure
MKQLFATTARGFEELLKVELTDLGAMDCQITQGGVHFHADEETQYRVLLWTRLASRILLP
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCCCEEEHHHHHHHHHHHHH
IVTCKVYSDLDLYSAVVGQNWLDYFDEKAHFMVDFNGTNREIRHTQFGAMRVKDGIVDYF
HHHHHHHCCHHHHHHHHCCHHHHHHCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHH
ERVGKPRPNVDKSQPDIRIHAYLNREELVISLDLSGDALHMRGYREDTGKAPLRETLAAA
HHHCCCCCCCCCCCCCEEEEEEECCCEEEEEEECCCCEEEECCCCCCCCCCHHHHHHHHH
IVLRSGWQLGTPLMDPMCGSGTLLIEAAQMQANIAPQLHRLHWGFDFWKGHNQQAWDKVK
HHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHCCCCHHHHHHHCCHHHHCCCCCHHHHHHC
GEAIELAEQTFNQNQKANFYGCDLDHRVLQKAKRNAQNAGVAHLIQWQQGDVAALKNPFV
CHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHCCCCE
EAKGTVICNPPYGERLGTTPALIALYSVFGQRLKQQFADWNVSIFSGEPALLDCLRLRSH
ECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHC
RQFKAKNGPLDCVQKNYHISARATASDENTQNSPEIDRTLTSSQVAVDFANRLQKNSKKI
CCCCCCCCCHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHH
EKWAKQQGINAYRLYDADLPEYNLAVDRYDDHIVVQEYAAPKNIDENKARQRLLDAVTAT
HHHHHHCCCCEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHE
LQVTGVETNKLILKVRQKQKGTNQYEKLANKGDYFYVNEYGAKLWVNLTDYLDTGLFLDH
EEEECCCCCHHHEEEHHHHCCCHHHHHHHCCCCEEEEEECCCEEEEEEHHHHCCCHHHHH
RLTRKMLGEMAKGKDVLNLFAYTGSATVHMALGGAKSTTTVDMSNTYLNWAEQNLLLNDL
HHHHHHHHHHHCCCHHEEEEEECCCCEEEEEECCCCCCEEEECCCCCHHHHHCCCEEECC
EGKQHKLIQADCLQWLARCDRQFDLIFVDPPTFSNSKRMEDSWDVQRDHIKLMTQLKRIL
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RPNGTIVFSNNKRGFKMDVEGLAALGLSAVEISAKTLPLDFERNKQIHNCWVVRLKADL
CCCCEEEECCCCCCEEECHHHHHHHCCCEEEEECEECCCCCCCCCCEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100