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The map label for this gene is metG

Identifier: 15602168

GI number: 15602168

Start: 354000

End: 356048

Strand: Direct

Name: metG

Synonym: PM0303

Alternate gene names: 15602168

Gene position: 354000-356048 (Clockwise)

Preceding gene: 15602166

Following gene: 15602169

Centisome position: 15.68

GC content: 40.51

Gene sequence:

>2049_bases
ATGGCAAATTCGGCACGCGATATTTTGGTCACTTGCGCCTTACCTTATGCAAATGGTGCAATTCATTTAGGGCATTTATT
AGAACATATTCAAGCAGATATTTGGGTGCGTTTCCAACGTATGCGTGGGCATAAAGTGCATTTTATTTGTGCAGATGATG
CGCATGGCACACCAATCATGTTAAATGCGGATAAATTAGGTATTACACCAGAAGCATTAATTGCTCGCTCGAAAGCGGAT
CATGTGGCGGATTTTGAAGGCTTTAATATTAGCTATGATAATTATCATTCCACTCACAGCCCAGAAAATGAAGCCTTAAC
AGCAGAAATGTATAAAAAATTACGTGCTAATGGCTTTATTAAAAGTCGTACGATTTCACAATTATTCGATCCAGAAAAAG
GCATGTTTTTACCTGATCGTTTTGTTAAAGGGACTTGCCCAAAATGTAAAGCAGAAGATCAATATGGTGATAACTGTGAG
GTCTGTTCATCCACTTATAGCCCAACGGAATTAATTAATCCGCGTTCTGTGGTTTCTGGTGCTACTCCCGTATTAAAAGA
ATCGGAGCATTTCTTCTTTGATTTACCTGCGTTTGAAGGCATGTTAAAAGCGTGGATCAAATCCGGTTCATTACAACCTG
AAATTGCCAACAAAATGCAAGAATGGTTTGAGTCGGGTTTACAACAATGGGATATTTCCCGTGATGCGCCGTATTTCGGC
TTTAAAATTCCGGATACAGAAGATAAATATTTCTATGTCTGGTTAGATGCCCCAATTGGATATATGGCATCGTTTAAAAA
TTTGTGTGATAAAACAGGCTTAAATTTTGACGATTTCTGGAAAAAAGACAGCTCGGCAGAGCTTTATCACTTCATCGGTA
AAGATATCGTGTATTTCCACAGTTTATTTTGGCCGGCGATGTTAGACGGTTGCGATTTACGCAAACCAACTAACGTGTTT
GCCCATGGTTATGTGACTGTAAATGGGGTGAAAATGTCAAAATCTCGTGGCACTTTTATTCAAGCGTCCACCTACTTACG
TCATTTAGCGCCAGAATATTTACGTTATTACTATGCGGCAAAATTGAATAACCGTATTGAAGATTTAGACTTAAATTTGG
AAGACTTTGTACAACGTTTAAATGCGGATGTGGTGAATAAATTTGTCAATCTGGCTTCACGCAGTGCCGGTTTTATCACG
AAGCGTTTTGATGGCAAATTATCAGCTGAGATTGCTGAACCTGAATTATTGGCAGAATTTGTGGAAAAAGCCGCGCAAAT
TGCGACTTATTATGAAGAACGCGAATTCGGTAAAGTCGTGCGTGAAGTGATGCAATTGGCGGATAAAGCGAATAAATACA
TTGATGACAAAGCGCCATGGGTGATGGCGAAAGAAGAGGGACGTGAGGCGGAATTACAAGCTGTTTGTTCAATGGCATTG
CAATTATTCCGCGTCTTAGCCATTTATTTAAAACCAGTGATTCCACAAATTATTGCGCGTGCGGAAGCCTTCTTACAGGA
TGAGTTAACGTGGGAAAGCTTAAATCGCCCGCTATTAAATCACGCAATTTTACCGTTTAAAGCGTTAGCACAGCGTTTAG
ACCCAAAACAGATTGAGGCAATTGTGAACGAAACCAAAGAACAATTTGTAGCCCAGCAGGCATTAGAGCAAAAAAATTCA
GCAAAAGCAGAACCCGCTAGCCAAGTGGAACCAATCGCAGAGACGATCAGCATTGAAGATTTCGCGAAATTAGATTTACG
TGTGGCGAAAGTGATGAAATGTGAAGCGGTGCCAGAATCAAACAAACTCTTACGTTTTGAGTTAGACTTAGGTGATCATA
CGCGTCAGGTGTTCTCTGGGATTAAAGAAGCATATAACAATCCTGCGGAATTAGAAGGGCGTTTTGTGGTGGTGATTGCC
AACCTTGCACCACGTAAAATGCGTTTTGGCGTATCAGAAGGGATGATTTTAAGTGCGGGGACAGGTGGAGCTGACTTGTT
CTTATTGAATGCCGATCAAGGTGTTAAACCTGGTATGCAGGTAAAATAA

Upstream 100 bases:

>100_bases
TGTTAAGCAGAAATGTGGAAAAGCGCGGTTAAAGTAGAAAAAATACTGCGAATAAGGTAACATAAGCGCCAATTTTTTGA
TGAAAAATAGGAATGATAAC

Downstream 100 bases:

>100_bases
CCTTGAGTTAGTTTTTTCATACAATAAAGTGCGGTAAAATTTTTGAATTTTTTACCGCACTTTTCCTTTCCTCCGTTTTT
GCAGGTGAAAACCTGTTATA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 682; Mature: 681

Protein sequence:

>682_residues
MANSARDILVTCALPYANGAIHLGHLLEHIQADIWVRFQRMRGHKVHFICADDAHGTPIMLNADKLGITPEALIARSKAD
HVADFEGFNISYDNYHSTHSPENEALTAEMYKKLRANGFIKSRTISQLFDPEKGMFLPDRFVKGTCPKCKAEDQYGDNCE
VCSSTYSPTELINPRSVVSGATPVLKESEHFFFDLPAFEGMLKAWIKSGSLQPEIANKMQEWFESGLQQWDISRDAPYFG
FKIPDTEDKYFYVWLDAPIGYMASFKNLCDKTGLNFDDFWKKDSSAELYHFIGKDIVYFHSLFWPAMLDGCDLRKPTNVF
AHGYVTVNGVKMSKSRGTFIQASTYLRHLAPEYLRYYYAAKLNNRIEDLDLNLEDFVQRLNADVVNKFVNLASRSAGFIT
KRFDGKLSAEIAEPELLAEFVEKAAQIATYYEEREFGKVVREVMQLADKANKYIDDKAPWVMAKEEGREAELQAVCSMAL
QLFRVLAIYLKPVIPQIIARAEAFLQDELTWESLNRPLLNHAILPFKALAQRLDPKQIEAIVNETKEQFVAQQALEQKNS
AKAEPASQVEPIAETISIEDFAKLDLRVAKVMKCEAVPESNKLLRFELDLGDHTRQVFSGIKEAYNNPAELEGRFVVVIA
NLAPRKMRFGVSEGMILSAGTGGADLFLLNADQGVKPGMQVK

Sequences:

>Translated_682_residues
MANSARDILVTCALPYANGAIHLGHLLEHIQADIWVRFQRMRGHKVHFICADDAHGTPIMLNADKLGITPEALIARSKAD
HVADFEGFNISYDNYHSTHSPENEALTAEMYKKLRANGFIKSRTISQLFDPEKGMFLPDRFVKGTCPKCKAEDQYGDNCE
VCSSTYSPTELINPRSVVSGATPVLKESEHFFFDLPAFEGMLKAWIKSGSLQPEIANKMQEWFESGLQQWDISRDAPYFG
FKIPDTEDKYFYVWLDAPIGYMASFKNLCDKTGLNFDDFWKKDSSAELYHFIGKDIVYFHSLFWPAMLDGCDLRKPTNVF
AHGYVTVNGVKMSKSRGTFIQASTYLRHLAPEYLRYYYAAKLNNRIEDLDLNLEDFVQRLNADVVNKFVNLASRSAGFIT
KRFDGKLSAEIAEPELLAEFVEKAAQIATYYEEREFGKVVREVMQLADKANKYIDDKAPWVMAKEEGREAELQAVCSMAL
QLFRVLAIYLKPVIPQIIARAEAFLQDELTWESLNRPLLNHAILPFKALAQRLDPKQIEAIVNETKEQFVAQQALEQKNS
AKAEPASQVEPIAETISIEDFAKLDLRVAKVMKCEAVPESNKLLRFELDLGDHTRQVFSGIKEAYNNPAELEGRFVVVIA
NLAPRKMRFGVSEGMILSAGTGGADLFLLNADQGVKPGMQVK
>Mature_681_residues
ANSARDILVTCALPYANGAIHLGHLLEHIQADIWVRFQRMRGHKVHFICADDAHGTPIMLNADKLGITPEALIARSKADH
VADFEGFNISYDNYHSTHSPENEALTAEMYKKLRANGFIKSRTISQLFDPEKGMFLPDRFVKGTCPKCKAEDQYGDNCEV
CSSTYSPTELINPRSVVSGATPVLKESEHFFFDLPAFEGMLKAWIKSGSLQPEIANKMQEWFESGLQQWDISRDAPYFGF
KIPDTEDKYFYVWLDAPIGYMASFKNLCDKTGLNFDDFWKKDSSAELYHFIGKDIVYFHSLFWPAMLDGCDLRKPTNVFA
HGYVTVNGVKMSKSRGTFIQASTYLRHLAPEYLRYYYAAKLNNRIEDLDLNLEDFVQRLNADVVNKFVNLASRSAGFITK
RFDGKLSAEIAEPELLAEFVEKAAQIATYYEEREFGKVVREVMQLADKANKYIDDKAPWVMAKEEGREAELQAVCSMALQ
LFRVLAIYLKPVIPQIIARAEAFLQDELTWESLNRPLLNHAILPFKALAQRLDPKQIEAIVNETKEQFVAQQALEQKNSA
KAEPASQVEPIAETISIEDFAKLDLRVAKVMKCEAVPESNKLLRFELDLGDHTRQVFSGIKEAYNNPAELEGRFVVVIAN
LAPRKMRFGVSEGMILSAGTGGADLFLLNADQGVKPGMQVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=634, Percent_Identity=28.0757097791798, Blast_Score=262, Evalue=1e-69,
Organism=Homo sapiens, GI24308436, Length=511, Percent_Identity=24.6575342465753, Blast_Score=153, Evalue=4e-37,
Organism=Escherichia coli, GI1788432, Length=684, Percent_Identity=67.2514619883041, Blast_Score=1001, Evalue=0.0,
Organism=Escherichia coli, GI1789455, Length=109, Percent_Identity=34.8623853211009, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17540794, Length=611, Percent_Identity=27.823240589198, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI212645096, Length=509, Percent_Identity=26.3261296660118, Blast_Score=158, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI71992781, Length=403, Percent_Identity=26.7990074441687, Blast_Score=123, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6321704, Length=579, Percent_Identity=27.2884283246978, Blast_Score=229, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6321610, Length=540, Percent_Identity=22.962962962963, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24655311, Length=505, Percent_Identity=29.3069306930693, Blast_Score=237, Evalue=2e-62,
Organism=Drosophila melanogaster, GI21358483, Length=512, Percent_Identity=24.609375, Blast_Score=139, Evalue=4e-33,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_PASMU (P57838)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245240.1
- ProteinModelPortal:   P57838
- GeneID:   1243650
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0303
- NMPDR:   fig|272843.1.peg.303
- HOGENOM:   HBG721667
- OMA:   CEAVPES
- ProtClustDB:   PRK00133
- BioCyc:   PMUL272843:PM0303-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 77051; Mature: 76920

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 334-334

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSARDILVTCALPYANGAIHLGHLLEHIQADIWVRFQRMRGHKVHFICADDAHGTPIM
CCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEE
LNADKLGITPEALIARSKADHVADFEGFNISYDNYHSTHSPENEALTAEMYKKLRANGFI
EECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCH
KSRTISQLFDPEKGMFLPDRFVKGTCPKCKAEDQYGDNCEVCSSTYSPTELINPRSVVSG
HHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCHHHCCCHHHHCC
ATPVLKESEHFFFDLPAFEGMLKAWIKSGSLQPEIANKMQEWFESGLQQWDISRDAPYFG
CCCHHCCCCCEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCE
FKIPDTEDKYFYVWLDAPIGYMASFKNLCDKTGLNFDDFWKKDSSAELYHFIGKDIVYFH
EECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHH
SLFWPAMLDGCDLRKPTNVFAHGYVTVNGVKMSKSRGTFIQASTYLRHLAPEYLRYYYAA
HHHHHHHHCCCCCCCCCCEEEEEEEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHH
KLNNRIEDLDLNLEDFVQRLNADVVNKFVNLASRSAGFITKRFDGKLSAEIAEPELLAEF
HHCCCHHHCCCCHHHHHHHHCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHH
VEKAAQIATYYEEREFGKVVREVMQLADKANKYIDDKAPWVMAKEEGREAELQAVCSMAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHH
QLFRVLAIYLKPVIPQIIARAEAFLQDELTWESLNRPLLNHAILPFKALAQRLDPKQIEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHCCCHHHHHHHCCHHHHHH
IVNETKEQFVAQQALEQKNSAKAEPASQVEPIAETISIEDFAKLDLRVAKVMKCEAVPES
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCC
NKLLRFELDLGDHTRQVFSGIKEAYNNPAELEGRFVVVIANLAPRKMRFGVSEGMILSAG
CCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHCCCCCCEEEECC
TGGADLFLLNADQGVKPGMQVK
CCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
ANSARDILVTCALPYANGAIHLGHLLEHIQADIWVRFQRMRGHKVHFICADDAHGTPIM
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEE
LNADKLGITPEALIARSKADHVADFEGFNISYDNYHSTHSPENEALTAEMYKKLRANGFI
EECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCH
KSRTISQLFDPEKGMFLPDRFVKGTCPKCKAEDQYGDNCEVCSSTYSPTELINPRSVVSG
HHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCHHHCCCHHHHCC
ATPVLKESEHFFFDLPAFEGMLKAWIKSGSLQPEIANKMQEWFESGLQQWDISRDAPYFG
CCCHHCCCCCEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCE
FKIPDTEDKYFYVWLDAPIGYMASFKNLCDKTGLNFDDFWKKDSSAELYHFIGKDIVYFH
EECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHH
SLFWPAMLDGCDLRKPTNVFAHGYVTVNGVKMSKSRGTFIQASTYLRHLAPEYLRYYYAA
HHHHHHHHCCCCCCCCCCEEEEEEEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHH
KLNNRIEDLDLNLEDFVQRLNADVVNKFVNLASRSAGFITKRFDGKLSAEIAEPELLAEF
HHCCCHHHCCCCHHHHHHHHCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHH
VEKAAQIATYYEEREFGKVVREVMQLADKANKYIDDKAPWVMAKEEGREAELQAVCSMAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHH
QLFRVLAIYLKPVIPQIIARAEAFLQDELTWESLNRPLLNHAILPFKALAQRLDPKQIEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHCCCHHHHHHHCCHHHHHH
IVNETKEQFVAQQALEQKNSAKAEPASQVEPIAETISIEDFAKLDLRVAKVMKCEAVPES
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCC
NKLLRFELDLGDHTRQVFSGIKEAYNNPAELEGRFVVVIANLAPRKMRFGVSEGMILSAG
CCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHCCCCCCEEEECC
TGGADLFLLNADQGVKPGMQVK
CCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100