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The map label for this gene is murC

Identifier: 15602008

GI number: 15602008

Start: 169228

End: 170676

Strand: Direct

Name: murC

Synonym: PM0143

Alternate gene names: 15602008

Gene position: 169228-170676 (Clockwise)

Preceding gene: 15602007

Following gene: 15602009

Centisome position: 7.5

GC content: 44.17

Gene sequence:

>1449_bases
ATGATGAATTCAAAATGTGGTAAAGCTTTTCAGCAACGGGTAAGAGATATGATCCCGGAAATGCGCCGTGTTCGTCAGAT
CCATTTTGTTGGTATTGGCGGCGCTGGTATGGGCGGAATTGCAGAAGTGTTATTAAATGAAGGTTATTTTGTCACTGGCT
CGGATATTATGGAAAGTGCGGTCACGGCACGCTTGACGTCCTTAGGTGCCAAGATTGCTTTCTCGCATGCGGCAGAAAAT
ATCGACGGGGCAAGTGTGGTTGTGGTGTCAAGTGCGATAAGAGCAGATAATGTTGAAGTCGTCGCAGCACAAGAAAGACG
TATTCCGGTTATTCAGCGTGCACAAATGTTGGCTGAAATTATGCGTTTTCGTCATGGTATTGCGGTGGCGGGTACACATG
GTAAGACGACGACTACAGCAATGATTTCAATGATTTATGCTGAAGCGGGTTTGGATCCGACTTTCGTAAATGGTGGATTA
GTGAAGTCTGCAGGGACAAATGCCCATTTAGGTTGTAGTCGTTACTTAATTGCCGAAGCGGATGAAAGTGATGCTTCATT
TTTACATTTGCAACCTATGGTGTCTGTAGTCACTAACATTGAACCCGATCATATGGAAACGTATCACGGCAGTTTTGAAG
AGATGAAACACACCTATGTGAATTTCTTACATAATTTGCCATTTTATGGACTGGCGGTGTTGTGTGCGGATGATGATGTA
TTAACTGAACTGACACCCAAAGTTGGACGCCAAGTGATTACCTATGGCTTCAGTGAAAAAGCGGATTATCGCATTGAAGA
TTATCAGCAAACAGGATTCCAAGGGCACTACACTGTCATTACACCAAGTGGTGAGCGCATTGATGTGTTGTTGAATGTAC
CAGGTCGCCATAATGCATTGAATGCCACGGCGGCGCTAGTGGTAGCGAAAGAAGAAGGTATTGGTAATGAAGCGATTCTT
GCAGCCTTAGCCGATTTCCAAGGAGCAGGACGTCGATTTGATCAACTTGGTCAATTTATTCGTCCAAATGGCAAGGTGAT
GTTAGTCGATGATTATGGTCATCATCCAACGGAAGTGGGCGTGACCATTCAAGCCGCACGCCAAGGTTGGGAAAATAAGC
GTATTGCGATGATTTTTCAACCGCATCGTTATTCAAGAACACGTGACTTATTTGATGATTTTGTCCAAGTGCTTTCGCAA
GTGGATGTCTTAATTATGTTAGATGTGTATGCGGCTGGTGAAACGCCGATTGCCGGCGCAGATAGCCGTTCTTTGTGTCG
TTCTATTCGTAACTTAGGTAAGGTCGATCCTATTTTTGTCTCTGACCATCGTCAGTTAGGAGAGATTCTAGATCAAGTTT
TACAAGATGGGGATTTAATTTTGGCACAGGGTGCGGGTAATGTGAGTAAAATTGCGCGTCAATTAGCAGAAACTTGGACC
AAAGAATAG

Upstream 100 bases:

>100_bases
CAAATGGCAATTAAAGCAAAAGCCATGGCAACCCCACTCGCCGCACAACGTGTCGCGGAAGTGATTATCGAAGAAGCGAA
TCAAGATTAGGAACGTGTAA

Downstream 100 bases:

>100_bases
GTGGTACAATGAAAAAAACATTAAAACAAGAAAAAATTGCAGTGTTATTAGGGGGCACTTCAGCAGAGCGTGAGGTTTCT
TTGGATTCAGGTCAGGCGGT

Product: UDP-N-acetylmuramate--L-alanine ligase

Products: NA

Alternate protein names: UDP-N-acetylmuramoyl-L-alanine synthetase

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MMNSKCGKAFQQRVRDMIPEMRRVRQIHFVGIGGAGMGGIAEVLLNEGYFVTGSDIMESAVTARLTSLGAKIAFSHAAEN
IDGASVVVVSSAIRADNVEVVAAQERRIPVIQRAQMLAEIMRFRHGIAVAGTHGKTTTTAMISMIYAEAGLDPTFVNGGL
VKSAGTNAHLGCSRYLIAEADESDASFLHLQPMVSVVTNIEPDHMETYHGSFEEMKHTYVNFLHNLPFYGLAVLCADDDV
LTELTPKVGRQVITYGFSEKADYRIEDYQQTGFQGHYTVITPSGERIDVLLNVPGRHNALNATAALVVAKEEGIGNEAIL
AALADFQGAGRRFDQLGQFIRPNGKVMLVDDYGHHPTEVGVTIQAARQGWENKRIAMIFQPHRYSRTRDLFDDFVQVLSQ
VDVLIMLDVYAAGETPIAGADSRSLCRSIRNLGKVDPIFVSDHRQLGEILDQVLQDGDLILAQGAGNVSKIARQLAETWT
KE

Sequences:

>Translated_482_residues
MMNSKCGKAFQQRVRDMIPEMRRVRQIHFVGIGGAGMGGIAEVLLNEGYFVTGSDIMESAVTARLTSLGAKIAFSHAAEN
IDGASVVVVSSAIRADNVEVVAAQERRIPVIQRAQMLAEIMRFRHGIAVAGTHGKTTTTAMISMIYAEAGLDPTFVNGGL
VKSAGTNAHLGCSRYLIAEADESDASFLHLQPMVSVVTNIEPDHMETYHGSFEEMKHTYVNFLHNLPFYGLAVLCADDDV
LTELTPKVGRQVITYGFSEKADYRIEDYQQTGFQGHYTVITPSGERIDVLLNVPGRHNALNATAALVVAKEEGIGNEAIL
AALADFQGAGRRFDQLGQFIRPNGKVMLVDDYGHHPTEVGVTIQAARQGWENKRIAMIFQPHRYSRTRDLFDDFVQVLSQ
VDVLIMLDVYAAGETPIAGADSRSLCRSIRNLGKVDPIFVSDHRQLGEILDQVLQDGDLILAQGAGNVSKIARQLAETWT
KE
>Mature_482_residues
MMNSKCGKAFQQRVRDMIPEMRRVRQIHFVGIGGAGMGGIAEVLLNEGYFVTGSDIMESAVTARLTSLGAKIAFSHAAEN
IDGASVVVVSSAIRADNVEVVAAQERRIPVIQRAQMLAEIMRFRHGIAVAGTHGKTTTTAMISMIYAEAGLDPTFVNGGL
VKSAGTNAHLGCSRYLIAEADESDASFLHLQPMVSVVTNIEPDHMETYHGSFEEMKHTYVNFLHNLPFYGLAVLCADDDV
LTELTPKVGRQVITYGFSEKADYRIEDYQQTGFQGHYTVITPSGERIDVLLNVPGRHNALNATAALVVAKEEGIGNEAIL
AALADFQGAGRRFDQLGQFIRPNGKVMLVDDYGHHPTEVGVTIQAARQGWENKRIAMIFQPHRYSRTRDLFDDFVQVLSQ
VDVLIMLDVYAAGETPIAGADSRSLCRSIRNLGKVDPIFVSDHRQLGEILDQVLQDGDLILAQGAGNVSKIARQLAETWT
KE

Specific function: Cell wall formation

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786279, Length=474, Percent_Identity=68.7763713080169, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI1790680, Length=486, Percent_Identity=28.1893004115226, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURC_PASMU (P57818)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245080.1
- ProteinModelPortal:   P57818
- SMR:   P57818
- PRIDE:   P57818
- GeneID:   1243490
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0143
- NMPDR:   fig|272843.1.peg.143
- HOGENOM:   HBG476594
- OMA:   YQPHRYS
- ProtClustDB:   PRK00421
- BioCyc:   PMUL272843:PM0143-MONOMER
- BRENDA:   6.3.2.8
- GO:   GO:0005737
- HAMAP:   MF_00046
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR016040
- InterPro:   IPR005758
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01082

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.8

Molecular weight: Translated: 52732; Mature: 52732

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNSKCGKAFQQRVRDMIPEMRRVRQIHFVGIGGAGMGGIAEVLLNEGYFVTGSDIMESA
CCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCCEEEECHHHHHHH
VTARLTSLGAKIAFSHAAENIDGASVVVVSSAIRADNVEVVAAQERRIPVIQRAQMLAEI
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCCCCEEEEEECCCCCHHHHHHHHHHHH
MRFRHGIAVAGTHGKTTTTAMISMIYAEAGLDPTFVNGGLVKSAGTNAHLGCSRYLIAEA
HHHHCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEECCCEEECCCCCCCCCCCEEEEEEC
DESDASFLHLQPMVSVVTNIEPDHMETYHGSFEEMKHTYVNFLHNLPFYGLAVLCADDDV
CCCCCCEEEHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCHHEEEEECCCHH
LTELTPKVGRQVITYGFSEKADYRIEDYQQTGFQGHYTVITPSGERIDVLLNVPGRHNAL
HHHHCHHHHHHHEEECCCCCCCCCCHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCCC
NATAALVVAKEEGIGNEAILAALADFQGAGRRFDQLGQFIRPNGKVMLVDDYGHHPTEVG
CCEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
VTIQAARQGWENKRIAMIFQPHRYSRTRDLFDDFVQVLSQVDVLIMLDVYAAGETPIAGA
EEEHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCC
DSRSLCRSIRNLGKVDPIFVSDHRQLGEILDQVLQDGDLILAQGAGNVSKIARQLAETWT
CHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHC
KE
CC
>Mature Secondary Structure
MMNSKCGKAFQQRVRDMIPEMRRVRQIHFVGIGGAGMGGIAEVLLNEGYFVTGSDIMESA
CCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCCEEEECHHHHHHH
VTARLTSLGAKIAFSHAAENIDGASVVVVSSAIRADNVEVVAAQERRIPVIQRAQMLAEI
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCCCCEEEEEECCCCCHHHHHHHHHHHH
MRFRHGIAVAGTHGKTTTTAMISMIYAEAGLDPTFVNGGLVKSAGTNAHLGCSRYLIAEA
HHHHCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEECCCEEECCCCCCCCCCCEEEEEEC
DESDASFLHLQPMVSVVTNIEPDHMETYHGSFEEMKHTYVNFLHNLPFYGLAVLCADDDV
CCCCCCEEEHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCHHEEEEECCCHH
LTELTPKVGRQVITYGFSEKADYRIEDYQQTGFQGHYTVITPSGERIDVLLNVPGRHNAL
HHHHCHHHHHHHEEECCCCCCCCCCHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCCC
NATAALVVAKEEGIGNEAILAALADFQGAGRRFDQLGQFIRPNGKVMLVDDYGHHPTEVG
CCEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
VTIQAARQGWENKRIAMIFQPHRYSRTRDLFDDFVQVLSQVDVLIMLDVYAAGETPIAGA
EEEHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCC
DSRSLCRSIRNLGKVDPIFVSDHRQLGEILDQVLQDGDLILAQGAGNVSKIARQLAETWT
CHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHC
KE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100