Click here to switch to the map view.

The map label for this gene is dsbE_2

Identifier: 15601893

GI number: 15601893

Start: 25486

End: 26052

Strand: Direct

Name: dsbE_2

Synonym: PM0028

Alternate gene names: 15601893

Gene position: 25486-26052 (Clockwise)

Preceding gene: 15601892

Following gene: 15601894

Centisome position: 1.13

GC content: 42.5

Gene sequence:

>567_bases
ATGAGTATGCTACATCAGCAAAAAAGAAAAAATCACTTTGTATTTTTACCGCTTGTGATCCTGTTAGCCGTATGCGCTTT
ATTATTCATCGGATTACAGCAGGATCCGCAGAAAATCGCTTCTGCCTTAATTGGTAAGCCTGTACCGACGTTTTCGCAAG
CCGATCTTTTGCGCACAGAAAGACGTGTGACTCAGCAGGATTTACCTCAACAGACCTTTTTACTCAATGTGTGGGGCAGT
TGGTGTGCGTATTGTAAAAAAGAGCATCCTTTTTTAATGCAATTAGCCAAATCGATGCCGATTGTGGGCTTAAATTACCG
TGATAACCCTCAAAATGCGTTGGCAATGTTGAATCAACTAGGTAATCCTTTTCAGCTTGTCATCAATGATAGTCGCGGGG
AATTAGCACTGAATTTAGGGGTAGATGGCGCCCCTGAGACCTATTTGATCGATCAATACGGTGTGATTCGTTATCGTTAT
TCAGGTCCATTAACGCCAGAGGTGTGGCAAGAAATGTTTATCCCAGAATGGCAAAAATTGGAGGCTGAAAATGCCAAAGT
GCGGTAA

Upstream 100 bases:

>100_bases
CTTTTAATGGCACTAGGCGCCTTGCTTGCCATATTTTCCTTGCGCAATAGCAATAAACTAAAACCACGTCGTCAATCAGA
TTGAATGAACAGGTGAGAGT

Downstream 100 bases:

>100_bases
GTTTTTACTGAGTTTTGTCTTTGTGATCAGTGTCTTTGCGCGGGCGGATATGGTGGATACTTACTCATTTAACAGCCCTG
AAGAACGGGTTCGTGCAGTG

Product: DsbE

Products: NA

Alternate protein names: Cytochrome c biogenesis protein NrfX

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MSMLHQQKRKNHFVFLPLVILLAVCALLFIGLQQDPQKIASALIGKPVPTFSQADLLRTERRVTQQDLPQQTFLLNVWGS
WCAYCKKEHPFLMQLAKSMPIVGLNYRDNPQNALAMLNQLGNPFQLVINDSRGELALNLGVDGAPETYLIDQYGVIRYRY
SGPLTPEVWQEMFIPEWQKLEAENAKVR

Sequences:

>Translated_188_residues
MSMLHQQKRKNHFVFLPLVILLAVCALLFIGLQQDPQKIASALIGKPVPTFSQADLLRTERRVTQQDLPQQTFLLNVWGS
WCAYCKKEHPFLMQLAKSMPIVGLNYRDNPQNALAMLNQLGNPFQLVINDSRGELALNLGVDGAPETYLIDQYGVIRYRY
SGPLTPEVWQEMFIPEWQKLEAENAKVR
>Mature_187_residues
SMLHQQKRKNHFVFLPLVILLAVCALLFIGLQQDPQKIASALIGKPVPTFSQADLLRTERRVTQQDLPQQTFLLNVWGSW
CAYCKKEHPFLMQLAKSMPIVGLNYRDNPQNALAMLNQLGNPFQLVINDSRGELALNLGVDGAPETYLIDQYGVIRYRYS
GPLTPEVWQEMFIPEWQKLEAENAKVR

Specific function: Could be involved in disulfide bond formation. Could catalyzes a late, reductive step in the assembly of periplasmic NrfA c-type cytochrome, probably the reduction of disulfide bonds of the apocytochrome c to allow covalent linkage with the heme. Possible

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein; Periplasmic side (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain

Homologues:

Organism=Escherichia coli, GI1788523, Length=185, Percent_Identity=44.8648648648649, Blast_Score=154, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NRFX_PASMU (Q9CPK9)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_244965.1
- ProteinModelPortal:   Q9CPK9
- SMR:   Q9CPK9
- GeneID:   1243375
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0028
- NMPDR:   fig|272843.1.peg.28
- HOGENOM:   HBG493509
- OMA:   DGTILYK
- ProtClustDB:   CLSK869940
- BioCyc:   PMUL272843:PM0028-MONOMER
- InterPro:   IPR004799
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335
- Gene3D:   G3DSA:3.40.30.10
- TIGRFAMs:   TIGR00385

Pfam domain/function: PF08534 Redoxin; SSF52833 Thiordxn-like_fd

EC number: NA

Molecular weight: Translated: 21510; Mature: 21379

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00194 THIOREDOXIN_1; PS51352 THIOREDOXIN_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xbb106e4)-;

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMLHQQKRKNHFVFLPLVILLAVCALLFIGLQQDPQKIASALIGKPVPTFSQADLLRTE
CCCHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
RRVTQQDLPQQTFLLNVWGSWCAYCKKEHPFLMQLAKSMPIVGLNYRDNPQNALAMLNQL
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEECCCCCCHHHHHHHHHHC
GNPFQLVINDSRGELALNLGVDGAPETYLIDQYGVIRYRYSGPLTPEVWQEMFIPEWQKL
CCCEEEEEECCCCCEEEEECCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHCCCCHHHH
EAENAKVR
CCCCCCCH
>Mature Secondary Structure 
SMLHQQKRKNHFVFLPLVILLAVCALLFIGLQQDPQKIASALIGKPVPTFSQADLLRTE
CCHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
RRVTQQDLPQQTFLLNVWGSWCAYCKKEHPFLMQLAKSMPIVGLNYRDNPQNALAMLNQL
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEECCCCCCHHHHHHHHHHC
GNPFQLVINDSRGELALNLGVDGAPETYLIDQYGVIRYRYSGPLTPEVWQEMFIPEWQKL
CCCEEEEEECCCCCEEEEECCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHCCCCHHHH
EAENAKVR
CCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11248100