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The map label for this gene is dacA

Identifier: 15674245

GI number: 15674245

Start: 2355791

End: 2357098

Strand: Direct

Name: dacA

Synonym: L139168

Alternate gene names: 15674245

Gene position: 2355791-2357098 (Clockwise)

Preceding gene: 15674240

Following gene: NA

Centisome position: 99.59

GC content: 36.31

Gene sequence:

>1308_bases
ATGAAAAAAATTGCAATTATTTTTTGTAGTTTCCTGATTAGTCTGTCAGTCCTTTCGGACTTTTCTGTCAGTGCTGCTAC
GACTCCAACGACAAATGATTTTACAGTTGCTGCTAAATCAGCAATTGCTATTGATGCCTCAAGTGGTAAAATTTTATACG
CGCAAAATTCTGATGATGCAAGCACTCGGATCGCCTCTATCACTAAATTACTGACAGCCTATTTAGTTTACAAAAATGTA
GCTGAAAATAAATTGACTTGGAATACTAAAGTTCCTATTTCAGATTATGCTTATGAATTAACTCAAAATTCTAACGCTTC
CAATATTCCCCTTGCTCAAAATGGTCAATATACTGTCAAAGATTTATTGAATGCTTTACTTCTTCCGTCAGCAAATTCTG
CTGCTGTCGCTTTAGCTGAAAAAATTGCTGGTTCAGAACCAAAATTTGTTGATATGATGACAAAACAAATGAAAGACTGG
GGAATTACTGACAGCCATCTCGTCAATGCTTCTGGCTTACCAAATGATGATTTAAACGGTCATATTTATCCAGGGTCTGG
TGCTTCAGCAACAAACACAATGAGCGCAAAGGCCGTAGCAACACTCAGTTATCATCTTATTCAAGATTTCCCAGAAATTC
TTCAAATCACTAAAAAAACAGAAATTCCTTTTGATACCGGCAATGAAGCAAAGATGACCTTAACTAATACCAATCAAATG
CTTAGTGGTTTTTCTACTTCAAGAACTGGTGTAGATGGCTTGAAAACAGGTTCAACAAGTTTTCAAATTGACTGTTTTGC
TGGAACAACAAATCAAAACGGTTTTAGAATCATTACGGTTGTTTTAGAAGCTACAGATCCTGCTGCTGACAACTCAACTC
CTTTCACTCTCACAAATCAACTGATGAACTATGTTTATGGTCATTGGCGTACGACCAGTCTTGTGCAAAAAAATCAATCC
TTAGATGATTTTAAAGAAATTACTGTGATTGATGGAAAAGAAAAATCTGTTCAACTCGTTGCTCCACAAAATATCACTCC
CGTTGTTCCTTACGCTACAGATGGTAGTGCAGATACAAAATCACTGACAGTCAAATTCAGTAAGGAAAAAACAGCCTCCG
TTGAAGCAACTGTGACTAAAAACCAAAAACTTGTCACTCTTTCTACTACAGTAAAGGATAGTTTAGGCTATCTTCCAAAT
TGTACCGCAGAACAAATTCCATTGGTTGCTAAGAAAACTGTCCCCCGTAGTAGCGCTCCAAAGGTTTTTTGGAATCATTT
TGTAAATTTTGTTAATGAAAAACTCTAA

Upstream 100 bases:

>100_bases
CTATTTTTTAGAATTGATTTTGAAAAAATAAAACTATTTAATTATCTATCAAGTTTTGAATCTATTGTACCTTACGAATA
GACTATGGTATAATCGAGTC

Downstream 100 bases:

>100_bases
AATTTTACTGACAGAAAACTTGTCAGTAAATTAACTCACAAAAAAAGCCGTCAATTTACTGACAGCTTTTTTATTATCCT
TTAGATTTTACAATTGCAAG

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MKKIAIIFCSFLISLSVLSDFSVSAATTPTTNDFTVAAKSAIAIDASSGKILYAQNSDDASTRIASITKLLTAYLVYKNV
AENKLTWNTKVPISDYAYELTQNSNASNIPLAQNGQYTVKDLLNALLLPSANSAAVALAEKIAGSEPKFVDMMTKQMKDW
GITDSHLVNASGLPNDDLNGHIYPGSGASATNTMSAKAVATLSYHLIQDFPEILQITKKTEIPFDTGNEAKMTLTNTNQM
LSGFSTSRTGVDGLKTGSTSFQIDCFAGTTNQNGFRIITVVLEATDPAADNSTPFTLTNQLMNYVYGHWRTTSLVQKNQS
LDDFKEITVIDGKEKSVQLVAPQNITPVVPYATDGSADTKSLTVKFSKEKTASVEATVTKNQKLVTLSTTVKDSLGYLPN
CTAEQIPLVAKKTVPRSSAPKVFWNHFVNFVNEKL

Sequences:

>Translated_435_residues
MKKIAIIFCSFLISLSVLSDFSVSAATTPTTNDFTVAAKSAIAIDASSGKILYAQNSDDASTRIASITKLLTAYLVYKNV
AENKLTWNTKVPISDYAYELTQNSNASNIPLAQNGQYTVKDLLNALLLPSANSAAVALAEKIAGSEPKFVDMMTKQMKDW
GITDSHLVNASGLPNDDLNGHIYPGSGASATNTMSAKAVATLSYHLIQDFPEILQITKKTEIPFDTGNEAKMTLTNTNQM
LSGFSTSRTGVDGLKTGSTSFQIDCFAGTTNQNGFRIITVVLEATDPAADNSTPFTLTNQLMNYVYGHWRTTSLVQKNQS
LDDFKEITVIDGKEKSVQLVAPQNITPVVPYATDGSADTKSLTVKFSKEKTASVEATVTKNQKLVTLSTTVKDSLGYLPN
CTAEQIPLVAKKTVPRSSAPKVFWNHFVNFVNEKL
>Mature_435_residues
MKKIAIIFCSFLISLSVLSDFSVSAATTPTTNDFTVAAKSAIAIDASSGKILYAQNSDDASTRIASITKLLTAYLVYKNV
AENKLTWNTKVPISDYAYELTQNSNASNIPLAQNGQYTVKDLLNALLLPSANSAAVALAEKIAGSEPKFVDMMTKQMKDW
GITDSHLVNASGLPNDDLNGHIYPGSGASATNTMSAKAVATLSYHLIQDFPEILQITKKTEIPFDTGNEAKMTLTNTNQM
LSGFSTSRTGVDGLKTGSTSFQIDCFAGTTNQNGFRIITVVLEATDPAADNSTPFTLTNQLMNYVYGHWRTTSLVQKNQS
LDDFKEITVIDGKEKSVQLVAPQNITPVVPYATDGSADTKSLTVKFSKEKTASVEATVTKNQKLVTLSTTVKDSLGYLPN
CTAEQIPLVAKKTVPRSSAPKVFWNHFVNFVNEKL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=406, Percent_Identity=25.615763546798, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786851, Length=436, Percent_Identity=24.0825688073394, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082022, Length=414, Percent_Identity=25.1207729468599, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 46994; Mature: 46994

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIAIIFCSFLISLSVLSDFSVSAATTPTTNDFTVAAKSAIAIDASSGKILYAQNSDDA
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECEEEEEECCCCEEEEEECCCCH
STRIASITKLLTAYLVYKNVAENKLTWNTKVPISDYAYELTQNSNASNIPLAQNGQYTVK
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHEEECCCCCCCCCCCCCCCCCHHH
DLLNALLLPSANSAAVALAEKIAGSEPKFVDMMTKQMKDWGITDSHLVNASGLPNDDLNG
HHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCC
HIYPGSGASATNTMSAKAVATLSYHLIQDFPEILQITKKTEIPFDTGNEAKMTLTNTNQM
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHH
LSGFSTSRTGVDGLKTGSTSFQIDCFAGTTNQNGFRIITVVLEATDPAADNSTPFTLTNQ
HHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHH
LMNYVYGHWRTTSLVQKNQSLDDFKEITVIDGKEKSVQLVAPQNITPVVPYATDGSADTK
HHHHHHCCCCHHHHHHCCCCCCHHHEEEEECCCCCCEEEECCCCCCEEEEECCCCCCCCE
SLTVKFSKEKTASVEATVTKNQKLVTLSTTVKDSLGYLPNCTAEQIPLVAKKTVPRSSAP
EEEEEECCCCCCEEEEEEECCCEEEEEEECHHHHCCCCCCCCHHHCCEEECCCCCCCCCC
KVFWNHFVNFVNEKL
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIAIIFCSFLISLSVLSDFSVSAATTPTTNDFTVAAKSAIAIDASSGKILYAQNSDDA
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECEEEEEECCCCEEEEEECCCCH
STRIASITKLLTAYLVYKNVAENKLTWNTKVPISDYAYELTQNSNASNIPLAQNGQYTVK
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHEEECCCCCCCCCCCCCCCCCHHH
DLLNALLLPSANSAAVALAEKIAGSEPKFVDMMTKQMKDWGITDSHLVNASGLPNDDLNG
HHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCC
HIYPGSGASATNTMSAKAVATLSYHLIQDFPEILQITKKTEIPFDTGNEAKMTLTNTNQM
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHH
LSGFSTSRTGVDGLKTGSTSFQIDCFAGTTNQNGFRIITVVLEATDPAADNSTPFTLTNQ
HHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHH
LMNYVYGHWRTTSLVQKNQSLDDFKEITVIDGKEKSVQLVAPQNITPVVPYATDGSADTK
HHHHHHCCCCHHHHHHCCCCCCHHHEEEEECCCCCCEEEECCCCCCEEEEECCCCCCCCE
SLTVKFSKEKTASVEATVTKNQKLVTLSTTVKDSLGYLPNCTAEQIPLVAKKTVPRSSAP
EEEEEECCCCCCEEEEEEECCCEEEEEEECHHHHCCCCCCCCHHHCCEEECCCCCCCCCC
KVFWNHFVNFVNEKL
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]