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The map label for this gene is ackA1

Identifier: 15673995

GI number: 15673995

Start: 2089923

End: 2091110

Strand: Reverse

Name: ackA1

Synonym: L73818

Alternate gene names: 15673995

Gene position: 2091110-2089923 (Counterclockwise)

Preceding gene: 15673996

Following gene: 15673994

Centisome position: 88.4

GC content: 37.79

Gene sequence:

>1188_bases
ATGACCAAAACATTAGCAGTTAACGCTGGTTCATCATCTATGAAATGGCAAATGTATGAAATGCCAGAAGAAAAAGTTCT
TGCCAAGGGATTAATCGAACGAATTGGTTTGAAAGATTCGATTGTGACCGTCAAATTTGGTGAGCAAAAAGAAGAACGCG
TTTTTGACATTCCAAATCATACAGAAGCAGTAGAAGTTTTACTTGAAGATTTAAAACGATTGAAAATAGTAGAGGAATTT
ACTGAAATTACAGGTGTAGGACATCGTGTGGTTGCTGGAGGTGAAATTTTCCAAAAATCAACAGTTGTTACACCAGAAGT
TTTGCAACAAGTTAAAGATTTGTCAGCTCTTGCTCCTTTGCATAATCCAGCAAATGCTGCAGGAATTGAAGCTTTTTTGA
ACTTACTACCTGATGCAACTTCAGTAGTAGTTTTTGATACAGCTTTCCATACAACGATGCCTGAAAAAGCATTCCGTTAT
CCATTGCCTAAAAAATATTATACAGATTATGCTGTCCGTAAATATGGGGCTCATGGAACTTCACACATGTATGTTTCACA
AGAAGCTGCAAAATTACTTGGAAAACCAATTGAAGAAACTAAAATTATCACTGCTCATATCGGGAATGGCGCATCACTGA
CAGCAGTAAAAGGTGGGAAATCAATTGATACTTCGATGGGATTCACACCACTTGCTGGAGTAATGATGGGAACACGTACA
GGTGAGATGGACCCTTCTGTTTTTCCATACCTTATTGAAAATGACTCTGAGTTAAAAGATGCTCAAGGTGTTGTTGATAT
GATGAACAAAGAATCTGGTCTCTATGGTGTATCTGGTATTTCATCAGATATGCGTGATATCATTGCTGCTAAAGATACAG
ATACTGATGCAAAACTTGCATTTGAGATGTATGTTGACCGAATTCAAAAATTTATTGGTCAATATTTGGCCGTTTTGAAT
GGGGCTGATGCTCTTGTCTTCACCGCTGGTATTGGTGAAAATTCGGTTCCTGTTCGTGAAGCAATTCTTTCTGGATTGAC
TTGGTTTGGAATTGAAGTTGACCCTGAGAAAAATGTCTTTGGCGTCGAGGGAGAAATTTCAACACCTCATTCACGCGTAA
AAGTTTTTGTGATTCCAACTGACGAAGAGCTTGTAATTGCTCGTGATGTTGAAGCACTTAAAAAATAA

Upstream 100 bases:

>100_bases
TCTTGAATTAAGCTCGTGAATCATGACCGAAAAACACAAAAGGAATTGTAATTGTGTTATAATTTAACCGTATTTCAAAT
TCAAGGAAGGTTTATTAAAT

Downstream 100 bases:

>100_bases
TTAATTAAAAGATTTTGTTCTAAAACAAAATCTTTTTTATTTTTAAAATGACATAAAATGATAAGTTCTGGGAATCCTTG
AGGGAGGAGAAATGAAATCA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase 1

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTKTLAVNAGSSSMKWQMYEMPEEKVLAKGLIERIGLKDSIVTVKFGEQKEERVFDIPNHTEAVEVLLEDLKRLKIVEEF
TEITGVGHRVVAGGEIFQKSTVVTPEVLQQVKDLSALAPLHNPANAAGIEAFLNLLPDATSVVVFDTAFHTTMPEKAFRY
PLPKKYYTDYAVRKYGAHGTSHMYVSQEAAKLLGKPIEETKIITAHIGNGASLTAVKGGKSIDTSMGFTPLAGVMMGTRT
GEMDPSVFPYLIENDSELKDAQGVVDMMNKESGLYGVSGISSDMRDIIAAKDTDTDAKLAFEMYVDRIQKFIGQYLAVLN
GADALVFTAGIGENSVPVREAILSGLTWFGIEVDPEKNVFGVEGEISTPHSRVKVFVIPTDEELVIARDVEALKK

Sequences:

>Translated_395_residues
MTKTLAVNAGSSSMKWQMYEMPEEKVLAKGLIERIGLKDSIVTVKFGEQKEERVFDIPNHTEAVEVLLEDLKRLKIVEEF
TEITGVGHRVVAGGEIFQKSTVVTPEVLQQVKDLSALAPLHNPANAAGIEAFLNLLPDATSVVVFDTAFHTTMPEKAFRY
PLPKKYYTDYAVRKYGAHGTSHMYVSQEAAKLLGKPIEETKIITAHIGNGASLTAVKGGKSIDTSMGFTPLAGVMMGTRT
GEMDPSVFPYLIENDSELKDAQGVVDMMNKESGLYGVSGISSDMRDIIAAKDTDTDAKLAFEMYVDRIQKFIGQYLAVLN
GADALVFTAGIGENSVPVREAILSGLTWFGIEVDPEKNVFGVEGEISTPHSRVKVFVIPTDEELVIARDVEALKK
>Mature_394_residues
TKTLAVNAGSSSMKWQMYEMPEEKVLAKGLIERIGLKDSIVTVKFGEQKEERVFDIPNHTEAVEVLLEDLKRLKIVEEFT
EITGVGHRVVAGGEIFQKSTVVTPEVLQQVKDLSALAPLHNPANAAGIEAFLNLLPDATSVVVFDTAFHTTMPEKAFRYP
LPKKYYTDYAVRKYGAHGTSHMYVSQEAAKLLGKPIEETKIITAHIGNGASLTAVKGGKSIDTSMGFTPLAGVMMGTRTG
EMDPSVFPYLIENDSELKDAQGVVDMMNKESGLYGVSGISSDMRDIIAAKDTDTDAKLAFEMYVDRIQKFIGQYLAVLNG
ADALVFTAGIGENSVPVREAILSGLTWFGIEVDPEKNVFGVEGEISTPHSRVKVFVIPTDEELVIARDVEALKK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=396, Percent_Identity=43.4343434343434, Blast_Score=317, Evalue=7e-88,
Organism=Escherichia coli, GI145693184, Length=398, Percent_Identity=40.4522613065327, Blast_Score=287, Evalue=8e-79,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA1_LACLA (Q9CE35)

Other databases:

- EMBL:   AE005176
- PIR:   E86876
- RefSeq:   NP_268170.1
- ProteinModelPortal:   Q9CE35
- SMR:   Q9CE35
- GeneID:   1115689
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L73818
- NMPDR:   fig|272623.1.peg.2067
- HOGENOM:   HBG285863
- OMA:   SSDFRDV
- ProtClustDB:   CLSK698067
- BioCyc:   LLAC272623:L73818-MONOMER
- BRENDA:   2.7.2.1
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 43138; Mature: 43007

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTLAVNAGSSSMKWQMYEMPEEKVLAKGLIERIGLKDSIVTVKFGEQKEERVFDIPNH
CCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEECCCCH
TEAVEVLLEDLKRLKIVEEFTEITGVGHRVVAGGEIFQKSTVVTPEVLQQVKDLSALAPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHCCCCCCCHHHHHHHHHHHHHCCC
HNPANAAGIEAFLNLLPDATSVVVFDTAFHTTMPEKAFRYPLPKKYYTDYAVRKYGAHGT
CCCCCHHHHHHHHHHCCCCCEEEEEECHHHCCCCHHHHCCCCCHHHHHHHHHHHHCCCCC
SHMYVSQEAAKLLGKPIEETKIITAHIGNGASLTAVKGGKSIDTSMGFTPLAGVMMGTRT
CEEEECHHHHHHHCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCC
GEMDPSVFPYLIENDSELKDAQGVVDMMNKESGLYGVSGISSDMRDIIAAKDTDTDAKLA
CCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHCCCCCCHHHHH
FEMYVDRIQKFIGQYLAVLNGADALVFTAGIGENSVPVREAILSGLTWFGIEVDPEKNVF
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCCEE
GVEGEISTPHSRVKVFVIPTDEELVIARDVEALKK
ECCCCCCCCCCCEEEEEEECCCCEEEEECHHHHCC
>Mature Secondary Structure 
TKTLAVNAGSSSMKWQMYEMPEEKVLAKGLIERIGLKDSIVTVKFGEQKEERVFDIPNH
CCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEECCCCH
TEAVEVLLEDLKRLKIVEEFTEITGVGHRVVAGGEIFQKSTVVTPEVLQQVKDLSALAPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHCCCCCCCHHHHHHHHHHHHHCCC
HNPANAAGIEAFLNLLPDATSVVVFDTAFHTTMPEKAFRYPLPKKYYTDYAVRKYGAHGT
CCCCCHHHHHHHHHHCCCCCEEEEEECHHHCCCCHHHHCCCCCHHHHHHHHHHHHCCCCC
SHMYVSQEAAKLLGKPIEETKIITAHIGNGASLTAVKGGKSIDTSMGFTPLAGVMMGTRT
CEEEECHHHHHHHCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCC
GEMDPSVFPYLIENDSELKDAQGVVDMMNKESGLYGVSGISSDMRDIIAAKDTDTDAKLA
CCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHCCCCCCHHHHH
FEMYVDRIQKFIGQYLAVLNGADALVFTAGIGENSVPVREAILSGLTWFGIEVDPEKNVF
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCCEE
GVEGEISTPHSRVKVFVIPTDEELVIARDVEALKK
ECCCCCCCCCCCEEEEEEECCCCEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471