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The map label for this gene is ackA2

Identifier: 15673994

GI number: 15673994

Start: 2088579

End: 2089766

Strand: Reverse

Name: ackA2

Synonym: L0224

Alternate gene names: 15673994

Gene position: 2089766-2088579 (Counterclockwise)

Preceding gene: 15673995

Following gene: 15673993

Centisome position: 88.34

GC content: 38.13

Gene sequence:

>1188_bases
ATGGAAAAAACGCTCGCTGTCAATGCAGGCTCCTCGTCATTAAAATGGCAACTTTACGAAATGCCGGAAGAAACCGTGAT
TGCTAAAGGAATTTTTGAACGTATTGGTTTGTCTGGCTCAATCTCAACAACTAAATTTAACAATGAAGAACACTTTAGAA
AACAAGAAATTGTTGACCATCGTCAAGCTGTTTTGTTATTAATGGATGAATTAATTCACTTTAAATTAATTCATGAATTC
CGTGAAATCACAGGAATTGGCCATCGTGTTGTTGCAGGTGGGGAATTTTTCAAAACTTCTACGGTTATTAGTCCTGAGGT
TTTGGCTGAGATTAAAAATCTTTCAACTTTGGCTCCTTTGCATAATCCTGCTAATGTTTTAGGGATTGAAGCTTTTCAAC
GTTTATTACCAGATGCACTTGCAGTTGCTGTTTTCGACACTGCTTTTCACAGTACTTTGCCAGAAAAGGCTTATCGTTAT
CCTATTCCAACAAAATATTATGAAGATTATTCAATCAGAAAATATGGAGCACATGGAACGTCACACATGTATGTCGCCCA
AGAAGCGGAAAAAGTTTTAGGAAAACCATTGGAAGATTTGAAGCTAATTACGGCTCATATCGGAAATGGAGCTTCAATTA
CAGCAATTGAAGCTGGAAAATCTGTTGATACTTCAATGGGATTTACACCACTTGCTGGTGTAATGATGGGAACACGTGCA
GGTGAAATGGACGCCTCAGTCATTCCTTATATGTTAGAGAGCGACCCAAGCCTTAGAAATGCTCAAGATGTGATTGACAT
TCTCAATAAAGACTCTGGAGTTCTTGGGGTGTCAGAACTCTCAAGTGATATGCGTGATCTTTCAGAAGCTGTGGCTAAAG
GAAATCCTAAAGCGATTCTTGCTTATGAAATGTATGTTGACCGTCTCAAGAAATTTATTGCCCAATATTTTGGAGTATTA
AATGGGGCTGATGCTCTTATTTTCACGGCTGGTGTTGGTGAAAATGATACAGCTGTTCGGACTGATGTAGTTAATGGACT
TTCTTGGTTTGGCATGGAAATTGATGAAAGTAAAAATGTTCGTGGAGCATTTGGTATCATTTCTAAACCAGAGTCTAAAG
TGAAAGTACTTGTTGTGCCAACAAACGAAGAATTGGTTATTGCTCGTGATGTTGAAGCTGCTAAATAA

Upstream 100 bases:

>100_bases
AATGATAAGTTCTGGGAATCCTTGAGGGAGGAGAAATGAAATCAGACATAAAATATGATATAATTTTAAGAATAAAACGC
ATACATTAAGGAGAGTAAGT

Downstream 100 bases:

>100_bases
TTAGATAAAATTTACTGACAGAAAATTGTCAGTAAATTTTTTTACTTATGAAAAAGCTGTCAGTAAAGTTTTGATAAATG
GTTTGTCTTCTATAAAAGAA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase 2

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MEKTLAVNAGSSSLKWQLYEMPEETVIAKGIFERIGLSGSISTTKFNNEEHFRKQEIVDHRQAVLLLMDELIHFKLIHEF
REITGIGHRVVAGGEFFKTSTVISPEVLAEIKNLSTLAPLHNPANVLGIEAFQRLLPDALAVAVFDTAFHSTLPEKAYRY
PIPTKYYEDYSIRKYGAHGTSHMYVAQEAEKVLGKPLEDLKLITAHIGNGASITAIEAGKSVDTSMGFTPLAGVMMGTRA
GEMDASVIPYMLESDPSLRNAQDVIDILNKDSGVLGVSELSSDMRDLSEAVAKGNPKAILAYEMYVDRLKKFIAQYFGVL
NGADALIFTAGVGENDTAVRTDVVNGLSWFGMEIDESKNVRGAFGIISKPESKVKVLVVPTNEELVIARDVEAAK

Sequences:

>Translated_395_residues
MEKTLAVNAGSSSLKWQLYEMPEETVIAKGIFERIGLSGSISTTKFNNEEHFRKQEIVDHRQAVLLLMDELIHFKLIHEF
REITGIGHRVVAGGEFFKTSTVISPEVLAEIKNLSTLAPLHNPANVLGIEAFQRLLPDALAVAVFDTAFHSTLPEKAYRY
PIPTKYYEDYSIRKYGAHGTSHMYVAQEAEKVLGKPLEDLKLITAHIGNGASITAIEAGKSVDTSMGFTPLAGVMMGTRA
GEMDASVIPYMLESDPSLRNAQDVIDILNKDSGVLGVSELSSDMRDLSEAVAKGNPKAILAYEMYVDRLKKFIAQYFGVL
NGADALIFTAGVGENDTAVRTDVVNGLSWFGMEIDESKNVRGAFGIISKPESKVKVLVVPTNEELVIARDVEAAK
>Mature_395_residues
MEKTLAVNAGSSSLKWQLYEMPEETVIAKGIFERIGLSGSISTTKFNNEEHFRKQEIVDHRQAVLLLMDELIHFKLIHEF
REITGIGHRVVAGGEFFKTSTVISPEVLAEIKNLSTLAPLHNPANVLGIEAFQRLLPDALAVAVFDTAFHSTLPEKAYRY
PIPTKYYEDYSIRKYGAHGTSHMYVAQEAEKVLGKPLEDLKLITAHIGNGASITAIEAGKSVDTSMGFTPLAGVMMGTRA
GEMDASVIPYMLESDPSLRNAQDVIDILNKDSGVLGVSELSSDMRDLSEAVAKGNPKAILAYEMYVDRLKKFIAQYFGVL
NGADALIFTAGVGENDTAVRTDVVNGLSWFGMEIDESKNVRGAFGIISKPESKVKVLVVPTNEELVIARDVEAAK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=399, Percent_Identity=41.1027568922306, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI145693184, Length=394, Percent_Identity=41.3705583756345, Blast_Score=280, Evalue=2e-76,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA2_LACLA (Q9CE36)

Other databases:

- EMBL:   AE005176
- PIR:   D86876
- RefSeq:   NP_268169.1
- ProteinModelPortal:   Q9CE36
- SMR:   Q9CE36
- GeneID:   1115688
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0224
- NMPDR:   fig|272623.1.peg.2066
- HOGENOM:   HBG285863
- OMA:   RERIMEQ
- ProtClustDB:   PRK00180
- BioCyc:   LLAC272623:L0224-MONOMER
- BRENDA:   2.7.2.1
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 43225; Mature: 43225

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKTLAVNAGSSSLKWQLYEMPEETVIAKGIFERIGLSGSISTTKFNNEEHFRKQEIVDH
CCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHH
RQAVLLLMDELIHFKLIHEFREITGIGHRVVAGGEFFKTSTVISPEVLAEIKNLSTLAPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEECCCCCCHHHHHHHHHHHHHCCC
HNPANVLGIEAFQRLLPDALAVAVFDTAFHSTLPEKAYRYPIPTKYYEDYSIRKYGAHGT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCEEEECCCCC
SHMYVAQEAEKVLGKPLEDLKLITAHIGNGASITAIEAGKSVDTSMGFTPLAGVMMGTRA
CEEEHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCC
GEMDASVIPYMLESDPSLRNAQDVIDILNKDSGVLGVSELSSDMRDLSEAVAKGNPKAIL
CCCCCHHCCHHCCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEE
AYEMYVDRLKKFIAQYFGVLNGADALIFTAGVGENDTAVRTDVVNGLSWFGMEIDESKNV
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCHHHCCCEECCCCCC
RGAFGIISKPESKVKVLVVPTNEELVIARDVEAAK
CEEECCCCCCCCCEEEEEEECCCCEEEEECCCCCC
>Mature Secondary Structure
MEKTLAVNAGSSSLKWQLYEMPEETVIAKGIFERIGLSGSISTTKFNNEEHFRKQEIVDH
CCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHH
RQAVLLLMDELIHFKLIHEFREITGIGHRVVAGGEFFKTSTVISPEVLAEIKNLSTLAPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEECCCCCCHHHHHHHHHHHHHCCC
HNPANVLGIEAFQRLLPDALAVAVFDTAFHSTLPEKAYRYPIPTKYYEDYSIRKYGAHGT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCEEEECCCCC
SHMYVAQEAEKVLGKPLEDLKLITAHIGNGASITAIEAGKSVDTSMGFTPLAGVMMGTRA
CEEEHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCC
GEMDASVIPYMLESDPSLRNAQDVIDILNKDSGVLGVSELSSDMRDLSEAVAKGNPKAIL
CCCCCHHCCHHCCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEE
AYEMYVDRLKKFIAQYFGVLNGADALIFTAGVGENDTAVRTDVVNGLSWFGMEIDESKNV
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCHHHCCCEECCCCCC
RGAFGIISKPESKVKVLVVPTNEELVIARDVEAAK
CEEECCCCCCCCCEEEEEEECCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471