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The map label for this gene is yuhD

Identifier: 15673975

GI number: 15673975

Start: 2072076

End: 2072897

Strand: Reverse

Name: yuhD

Synonym: L55507

Alternate gene names: 15673975

Gene position: 2072897-2072076 (Counterclockwise)

Preceding gene: 15673977

Following gene: 15673973

Centisome position: 87.63

GC content: 32.12

Gene sequence:

>822_bases
ATGTTAAAAAAAATTGAAAAAGCTTATTTACTTTTAGAAGAAAATGTGGAAATGTTGAGATGTCCAATTTGTCATGGAAA
ATTTCAATTGGCAGCCTATGCCTTAAAATGTGAAAATAATCATACTTATAATTTAAACAAGAAAGGTTATGTTAATTTTT
TACAAACAAAAGCTGACACAGAGCATTATACGCGTAAAATGTTTGAACCACGTAGACGCCTAATTCAAGCAGGGATGTAT
CAAAATTTACTGACAGAAATTCAAAAATCATTTGTCAGTGGAAATTTACTTGATGTTGGAACAGGTGAAGGTTCATTTTT
AGAACTCCTAGAGGGTGCGGGGGCGAAGTTTGCTTTTGATATTGCTAAAGATGGAATTGAAATGGCAACCGAATTAGATA
CGGAAAGTTTTCTGTCCTTAGCTGATTTAACTAATCTCCCTTTTGCTGACGAAAGTCTGTCAGTAATTTTGAATATTTTT
ACGCCTTCAAATTATGCAGAATTTCATCGAGTTCTGACAGAAAATGGCCGGGTCATTAAAATTATTCCTGACAGAAATTA
TTTACATGAGTTGCGAGAAGTTTATCAACTTCCAGTTGATTATGATAATCAAGCAGTTATTGAGCGTTTTAAAGAAGAAT
TTCCTAAGAATACTCAACAAACAATTGATTATACTTTTGAAATTCCAGAAAATTTGCGTCAAGATTTCTTATTGATGAGT
CCATTAGAATGGTCAGTTTCTGAGGAAAGAAAAAAATTTGCAAAGGAAAATCCGCCAAAAACAGCGAGAATTCATGTGCA
AATTTTAATTGGAATAAAATAA

Upstream 100 bases:

>100_bases
TACTAAAAATATTATAACATGATTATCTTGTTTAACTTAAAAAAATTACAAGAATTATTTTGAATAAAAAGAATGAAAGT
GATTATGCTAGAATAGAACT

Downstream 100 bases:

>100_bases
AAAACTGTCTATTAGGCAGTTTTTATTTCATCATAAATTTAATCGCTTTAGCAATTCCTTTTTTCACGGAAATATAAAGA
GCAAGCGAGAGAACAATAGA

Product: hypothetical protein

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MLKKIEKAYLLLEENVEMLRCPICHGKFQLAAYALKCENNHTYNLNKKGYVNFLQTKADTEHYTRKMFEPRRRLIQAGMY
QNLLTEIQKSFVSGNLLDVGTGEGSFLELLEGAGAKFAFDIAKDGIEMATELDTESFLSLADLTNLPFADESLSVILNIF
TPSNYAEFHRVLTENGRVIKIIPDRNYLHELREVYQLPVDYDNQAVIERFKEEFPKNTQQTIDYTFEIPENLRQDFLLMS
PLEWSVSEERKKFAKENPPKTARIHVQILIGIK

Sequences:

>Translated_273_residues
MLKKIEKAYLLLEENVEMLRCPICHGKFQLAAYALKCENNHTYNLNKKGYVNFLQTKADTEHYTRKMFEPRRRLIQAGMY
QNLLTEIQKSFVSGNLLDVGTGEGSFLELLEGAGAKFAFDIAKDGIEMATELDTESFLSLADLTNLPFADESLSVILNIF
TPSNYAEFHRVLTENGRVIKIIPDRNYLHELREVYQLPVDYDNQAVIERFKEEFPKNTQQTIDYTFEIPENLRQDFLLMS
PLEWSVSEERKKFAKENPPKTARIHVQILIGIK
>Mature_273_residues
MLKKIEKAYLLLEENVEMLRCPICHGKFQLAAYALKCENNHTYNLNKKGYVNFLQTKADTEHYTRKMFEPRRRLIQAGMY
QNLLTEIQKSFVSGNLLDVGTGEGSFLELLEGAGAKFAFDIAKDGIEMATELDTESFLSLADLTNLPFADESLSVILNIF
TPSNYAEFHRVLTENGRVIKIIPDRNYLHELREVYQLPVDYDNQAVIERFKEEFPKNTQQTIDYTFEIPENLRQDFLLMS
PLEWSVSEERKKFAKENPPKTARIHVQILIGIK

Specific function: Specifically Methylates The Guanosine In Position 745 Of 23s rRNA. [C]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=246, Percent_Identity=26.8292682926829, Blast_Score=91, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 31610; Mature: 31610

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKIEKAYLLLEENVEMLRCPICHGKFQLAAYALKCENNHTYNLNKKGYVNFLQTKADT
CCCHHHHHHHHHHCCHHHEECCCCCCCCEEEEEEEEECCCCEEECCCCCCHHHHHHCCCH
EHYTRKMFEPRRRLIQAGMYQNLLTEIQKSFVSGNLLDVGTGEGSFLELLEGAGAKFAFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCCCEEEHH
IAKDGIEMATELDTESFLSLADLTNLPFADESLSVILNIFTPSNYAEFHRVLTENGRVIK
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEE
IIPDRNYLHELREVYQLPVDYDNQAVIERFKEEFPKNTQQTIDYTFEIPENLRQDFLLMS
EECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHEEEEEECCHHHHHHEEEEC
PLEWSVSEERKKFAKENPPKTARIHVQILIGIK
CCCCCHHHHHHHHHHCCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MLKKIEKAYLLLEENVEMLRCPICHGKFQLAAYALKCENNHTYNLNKKGYVNFLQTKADT
CCCHHHHHHHHHHCCHHHEECCCCCCCCEEEEEEEEECCCCEEECCCCCCHHHHHHCCCH
EHYTRKMFEPRRRLIQAGMYQNLLTEIQKSFVSGNLLDVGTGEGSFLELLEGAGAKFAFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCCCEEEHH
IAKDGIEMATELDTESFLSLADLTNLPFADESLSVILNIFTPSNYAEFHRVLTENGRVIK
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEE
IIPDRNYLHELREVYQLPVDYDNQAVIERFKEEFPKNTQQTIDYTFEIPENLRQDFLLMS
EECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHEEEEEECCHHHHHHEEEEC
PLEWSVSEERKKFAKENPPKTARIHVQILIGIK
CCCCCHHHHHHHHHHCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377 [H]