Click here to switch to the map view.

The map label for this gene is birA2

Identifier: 15673885

GI number: 15673885

Start: 1972948

End: 1973700

Strand: Direct

Name: birA2

Synonym: L0192

Alternate gene names: 15673885

Gene position: 1972948-1973700 (Clockwise)

Preceding gene: 15673866

Following gene: 15673912

Centisome position: 83.4

GC content: 34.4

Gene sequence:

>753_bases
ATGAACGACAAACTTGAAAAATCTATTATTTTACAAAAAAATCCTTGGCTGACCGATGTAAAAATTGTCGCTGAATCTGA
ATCAACTCAAAAAGATGCTAAATTAGACGGACAAGAAAATTGTCTTTATTTAACTGAAAGACAAAATAAAACTTATGGTC
GTTTTGGCCGTCAGTATTTTGCTGCGCCGTCTGGCGGAATTTATATGAGTTTGTCACTCAAGCCCAAAGTTGAACTAGAA
AAACTTCCAGAGTTCACTCTGCTGACAGGAGCTGCCATTGTGTCAGCAATCGAAAAATTAACTGACAAAAAGCCTCAAAT
TAAATGGGTTAACGATATTTATCTAGACGATAAAAAATTTGTTGGTATTCTTGCCGAAACATCACTTAATCCAACTCAAG
AAGCTCAAGTTATCATTGGTGTCGGAATTAATTTTTCTATTTCCCAATTTCCAACTGAAATCGAAGAAAGAGCCACTTCT
TTATTTTATAATGAACAAGCAAACATCAGTCGTTCGGAATTAATTGCCAAAATCTGGTCTGAATTTCATCGTTTATCAGA
GGGTTCATTTTTTGAAATTTATAAATCTCACTCATTTATTCTCGGCAAACAAGTTGAATTTGTTCAATCTGGACAAACTT
TTTCCGGAAGAGCCACAGATTTAACCCCTAAAGGTGAGCTTATCGTAAATTTAGATAATGGTCAGCAAAAAATACTTTCC
AGTGGCGAAATCTCACTAAAAAAATGGACTTAA

Upstream 100 bases:

>100_bases
CTATAAAAAATAGATAACTTTGAACTTGCGCAAGTACAGAAACGTAATTTCCTAATTTTTCCTCTCGCTTGCAGTCGACT
TCACATGGTATAATAAAAAA

Downstream 100 bases:

>100_bases
TCAGTCCATTTTTTCAGTTATTATTATTATATTATATAGGAGGCCAGTTTTTGGTCATGGTGGACCAGAATTATTTTACT
GACAGAACTTTCTGTCAGTA

Product: bifunctional protein BirA

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MNDKLEKSIILQKNPWLTDVKIVAESESTQKDAKLDGQENCLYLTERQNKTYGRFGRQYFAAPSGGIYMSLSLKPKVELE
KLPEFTLLTGAAIVSAIEKLTDKKPQIKWVNDIYLDDKKFVGILAETSLNPTQEAQVIIGVGINFSISQFPTEIEERATS
LFYNEQANISRSELIAKIWSEFHRLSEGSFFEIYKSHSFILGKQVEFVQSGQTFSGRATDLTPKGELIVNLDNGQQKILS
SGEISLKKWT

Sequences:

>Translated_250_residues
MNDKLEKSIILQKNPWLTDVKIVAESESTQKDAKLDGQENCLYLTERQNKTYGRFGRQYFAAPSGGIYMSLSLKPKVELE
KLPEFTLLTGAAIVSAIEKLTDKKPQIKWVNDIYLDDKKFVGILAETSLNPTQEAQVIIGVGINFSISQFPTEIEERATS
LFYNEQANISRSELIAKIWSEFHRLSEGSFFEIYKSHSFILGKQVEFVQSGQTFSGRATDLTPKGELIVNLDNGQQKILS
SGEISLKKWT
>Mature_250_residues
MNDKLEKSIILQKNPWLTDVKIVAESESTQKDAKLDGQENCLYLTERQNKTYGRFGRQYFAAPSGGIYMSLSLKPKVELE
KLPEFTLLTGAAIVSAIEKLTDKKPQIKWVNDIYLDDKKFVGILAETSLNPTQEAQVIIGVGINFSISQFPTEIEERATS
LFYNEQANISRSELIAKIWSEFHRLSEGSFFEIYKSHSFILGKQVEFVQSGQTFSGRATDLTPKGELIVNLDNGQQKILS
SGEISLKKWT

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=215, Percent_Identity=25.1162790697674, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 28210; Mature: 28210

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKLEKSIILQKNPWLTDVKIVAESESTQKDAKLDGQENCLYLTERQNKTYGRFGRQYF
CCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCHHHHCCEEE
AAPSGGIYMSLSLKPKVELEKLPEFTLLTGAAIVSAIEKLTDKKPQIKWVNDIYLDDKKF
CCCCCCEEEEEECCCCCCHHHCCCEEHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCE
VGILAETSLNPTQEAQVIIGVGINFSISQFPTEIEERATSLFYNEQANISRSELIAKIWS
EEEEEECCCCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EFHRLSEGSFFEIYKSHSFILGKQVEFVQSGQTFSGRATDLTPKGELIVNLDNGQQKILS
HHHHCCCCCEEEEECCCCEEECCEEHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHHHHC
SGEISLKKWT
CCCEEEEECC
>Mature Secondary Structure
MNDKLEKSIILQKNPWLTDVKIVAESESTQKDAKLDGQENCLYLTERQNKTYGRFGRQYF
CCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCHHHHCCEEE
AAPSGGIYMSLSLKPKVELEKLPEFTLLTGAAIVSAIEKLTDKKPQIKWVNDIYLDDKKF
CCCCCCEEEEEECCCCCCHHHCCCEEHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCE
VGILAETSLNPTQEAQVIIGVGINFSISQFPTEIEERATSLFYNEQANISRSELIAKIWS
EEEEEECCCCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EFHRLSEGSFFEIYKSHSFILGKQVEFVQSGQTFSGRATDLTPKGELIVNLDNGQQKILS
HHHHCCCCCEEEEECCCCEEECCEEHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHHHHC
SGEISLKKWT
CCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]