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The map label for this gene is prsB

Identifier: 15673839

GI number: 15673839

Start: 1926020

End: 1926985

Strand: Direct

Name: prsB

Synonym: L109335

Alternate gene names: 15673839

Gene position: 1926020-1926985 (Clockwise)

Preceding gene: 15673827

Following gene: 15673841

Centisome position: 81.42

GC content: 39.44

Gene sequence:

>966_bases
GTGGTTTACTCTGATTCACACTTAAAGCTTTTTGCCTTAAACTCAAACCCTGGTCTTGCTGAGAAAATTAGCCAGTTTAC
AGGAGTTCCTCTAGGAAAACTTTCTTCAAAACAGTTTTCCGACGGTGAAATTATGATTAACATGGAAGAAAGTGTCCGTA
GTCAAGACATCTTTATTGTTCAATCAACAAGCTGCCCTGTTAACGATCACCTTTGGGAATTACTAATCATGATTGACGCA
TGTAAACGTGCTTCTGCTCGTACAATTAATGTTGTTATCCCATACTTTGGATATGCTCGTCAAGACCGTACTGCGACTTC
TCGTGAACCAATCACAGCTAAATTAGTTGCTGATATGATTGTTAAAGCTGGAGCTGACCGCTTATTAACCCTCGATATTC
ACGCTGTCCAAGTACAAGGTTTCTTTGATATCCCTGTAGACAACTTGTTTACTGTCCCACTTTTTGCTGACTACTACCGT
AAGAGTGGATTATTTGGTGATGACGTGATTGTTGTTGCACCAAAAAATTCTGGGATTAAACGTGCACGCTCACTTGCTGA
ATACTTAGAGTCAGCAATTGCCATTGTTGACTATGAAGATGATGACAAAAACCGTGAAAACGGATATGTTATCGGTAATG
TTTCAGGTAAAAAAGTTATTCTTATCGATGATATTTTGAACACCGGGGTCACTTTTGCCAACGCTGCAAATGTTGTACGC
GAAGCAGGAGCAAGTGAAATTTATGCAGTTGCCAGCCACGGATTATTTACTAGCGGAGCCGCTGATGTTCTTGAAAAAGT
AGATATCAAAGAAATTTTGGTCACAGATTCTGTTGTGACAAACCATCGCAAACCTTTAAATGTAAAATATTTGAGTGCAG
CTGAATACTTAGCCAGTGCTATTCTTCGTATCCACGAAGGACGTCCAGTTTCTCCACTTTTTGAACATGAAAAACCTCAA
GATTAA

Upstream 100 bases:

>100_bases
ATTAATTCATAAAGATATTTACAGATATAGACTATGAAAATCACTATTATTTTGCTAAAATAGAACTATATTATTTCATA
AAAGAAAATGGAGAGAAATC

Downstream 100 bases:

>100_bases
AGTAATTCTTATAGAGACACTTTAAGCAATTGAAAAATACCAACACTAAGTTGGTATTTTTATTTTTGATATATTAATTT
ATAAAATAAAAGTCATCTCA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK 2; Phosphoribosyl pyrophosphate synthase 2; P-Rib-PP synthase 2; PRPP synthase 2

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MVYSDSHLKLFALNSNPGLAEKISQFTGVPLGKLSSKQFSDGEIMINMEESVRSQDIFIVQSTSCPVNDHLWELLIMIDA
CKRASARTINVVIPYFGYARQDRTATSREPITAKLVADMIVKAGADRLLTLDIHAVQVQGFFDIPVDNLFTVPLFADYYR
KSGLFGDDVIVVAPKNSGIKRARSLAEYLESAIAIVDYEDDDKNRENGYVIGNVSGKKVILIDDILNTGVTFANAANVVR
EAGASEIYAVASHGLFTSGAADVLEKVDIKEILVTDSVVTNHRKPLNVKYLSAAEYLASAILRIHEGRPVSPLFEHEKPQ
D

Sequences:

>Translated_321_residues
MVYSDSHLKLFALNSNPGLAEKISQFTGVPLGKLSSKQFSDGEIMINMEESVRSQDIFIVQSTSCPVNDHLWELLIMIDA
CKRASARTINVVIPYFGYARQDRTATSREPITAKLVADMIVKAGADRLLTLDIHAVQVQGFFDIPVDNLFTVPLFADYYR
KSGLFGDDVIVVAPKNSGIKRARSLAEYLESAIAIVDYEDDDKNRENGYVIGNVSGKKVILIDDILNTGVTFANAANVVR
EAGASEIYAVASHGLFTSGAADVLEKVDIKEILVTDSVVTNHRKPLNVKYLSAAEYLASAILRIHEGRPVSPLFEHEKPQ
D
>Mature_321_residues
MVYSDSHLKLFALNSNPGLAEKISQFTGVPLGKLSSKQFSDGEIMINMEESVRSQDIFIVQSTSCPVNDHLWELLIMIDA
CKRASARTINVVIPYFGYARQDRTATSREPITAKLVADMIVKAGADRLLTLDIHAVQVQGFFDIPVDNLFTVPLFADYYR
KSGLFGDDVIVVAPKNSGIKRARSLAEYLESAIAIVDYEDDDKNRENGYVIGNVSGKKVILIDDILNTGVTFANAANVVR
EAGASEIYAVASHGLFTSGAADVLEKVDIKEILVTDSVVTNHRKPLNVKYLSAAEYLASAILRIHEGRPVSPLFEHEKPQ
D

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family

Homologues:

Organism=Homo sapiens, GI28557709, Length=313, Percent_Identity=40.2555910543131, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI4506127, Length=313, Percent_Identity=39.6166134185303, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI4506129, Length=310, Percent_Identity=39.0322580645161, Blast_Score=232, Evalue=3e-61,
Organism=Homo sapiens, GI84875539, Length=313, Percent_Identity=38.6581469648562, Blast_Score=228, Evalue=6e-60,
Organism=Homo sapiens, GI4506133, Length=347, Percent_Identity=34.8703170028818, Blast_Score=184, Evalue=9e-47,
Organism=Homo sapiens, GI194018537, Length=345, Percent_Identity=33.9130434782609, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI310128524, Length=140, Percent_Identity=34.2857142857143, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI310115209, Length=140, Percent_Identity=34.2857142857143, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI310118259, Length=140, Percent_Identity=34.2857142857143, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI310119946, Length=140, Percent_Identity=34.2857142857143, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1787458, Length=314, Percent_Identity=44.2675159235669, Blast_Score=258, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI25149168, Length=313, Percent_Identity=40.5750798722045, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17554702, Length=313, Percent_Identity=40.5750798722045, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI71989924, Length=313, Percent_Identity=40.5750798722045, Blast_Score=234, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI17554704, Length=310, Percent_Identity=40.6451612903226, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17570245, Length=341, Percent_Identity=32.5513196480938, Blast_Score=181, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6319403, Length=312, Percent_Identity=41.025641025641, Blast_Score=238, Evalue=9e-64,
Organism=Saccharomyces cerevisiae, GI6320946, Length=316, Percent_Identity=41.4556962025316, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321776, Length=313, Percent_Identity=38.9776357827476, Blast_Score=211, Evalue=9e-56,
Organism=Saccharomyces cerevisiae, GI6322667, Length=188, Percent_Identity=38.8297872340425, Blast_Score=152, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6324511, Length=98, Percent_Identity=41.8367346938776, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355239, Length=313, Percent_Identity=40.2555910543131, Blast_Score=235, Evalue=3e-62,
Organism=Drosophila melanogaster, GI45551540, Length=336, Percent_Identity=37.5, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24651458, Length=365, Percent_Identity=31.2328767123288, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24651456, Length=365, Percent_Identity=31.2328767123288, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI281362873, Length=360, Percent_Identity=31.3888888888889, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24651454, Length=360, Percent_Identity=31.3888888888889, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24651462, Length=202, Percent_Identity=36.6336633663366, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24651464, Length=202, Percent_Identity=36.6336633663366, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI45552010, Length=200, Percent_Identity=37, Blast_Score=130, Evalue=8e-31,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KPRS2_LACLA (Q9CEI4)

Other databases:

- EMBL:   AE005176
- PIR:   A86857
- RefSeq:   NP_268014.1
- ProteinModelPortal:   Q9CEI4
- SMR:   Q9CEI4
- GeneID:   1115522
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L109335
- NMPDR:   fig|272623.1.peg.1903
- HOGENOM:   HBG519284
- OMA:   YARQDRT
- ProtClustDB:   PRK02458
- BioCyc:   LLAC272623:L109335-MONOMER
- BRENDA:   2.7.6.1
- GO:   GO:0005737
- HAMAP:   MF_00583_B
- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01251

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.7.6.1

Molecular weight: Translated: 35279; Mature: 35279

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00114 PRPP_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYSDSHLKLFALNSNPGLAEKISQFTGVPLGKLSSKQFSDGEIMINMEESVRSQDIFIV
CCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHCCCCEEEEE
QSTSCPVNDHLWELLIMIDACKRASARTINVVIPYFGYARQDRTATSREPITAKLVADMI
ECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHH
VKAGADRLLTLDIHAVQVQGFFDIPVDNLFTVPLFADYYRKSGLFGDDVIVVAPKNSGIK
HHCCCCEEEEEEEEEEEEEEEEECCCCCEEEHHHHHHHHHHCCCCCCCEEEEECCCCCHH
RARSLAEYLESAIAIVDYEDDDKNRENGYVIGNVSGKKVILIDDILNTGVTFANAANVVR
HHHHHHHHHHHHEEEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHCCCCHHHHHHHHH
EAGASEIYAVASHGLFTSGAADVLEKVDIKEILVTDSVVTNHRKPLNVKYLSAAEYLASA
HCCCCCEEEECCCCCCCCCHHHHHHHCCHHHHHEECHHHHCCCCCCCEEEHHHHHHHHHH
ILRIHEGRPVSPLFEHEKPQD
HHHHCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MVYSDSHLKLFALNSNPGLAEKISQFTGVPLGKLSSKQFSDGEIMINMEESVRSQDIFIV
CCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHCCCCEEEEE
QSTSCPVNDHLWELLIMIDACKRASARTINVVIPYFGYARQDRTATSREPITAKLVADMI
ECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHH
VKAGADRLLTLDIHAVQVQGFFDIPVDNLFTVPLFADYYRKSGLFGDDVIVVAPKNSGIK
HHCCCCEEEEEEEEEEEEEEEEECCCCCEEEHHHHHHHHHHCCCCCCCEEEEECCCCCHH
RARSLAEYLESAIAIVDYEDDDKNRENGYVIGNVSGKKVILIDDILNTGVTFANAANVVR
HHHHHHHHHHHHEEEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHCCCCHHHHHHHHH
EAGASEIYAVASHGLFTSGAADVLEKVDIKEILVTDSVVTNHRKPLNVKYLSAAEYLASA
HCCCCCEEEECCCCCCCCCHHHHHHHCCHHHHHEECHHHHCCCCCCCEEEHHHHHHHHHH
ILRIHEGRPVSPLFEHEKPQD
HHHHCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471