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The map label for this gene is nifS

Identifier: 15673841

GI number: 15673841

Start: 1927778

End: 1928905

Strand: Direct

Name: nifS

Synonym: L111126

Alternate gene names: 15673841

Gene position: 1927778-1928905 (Clockwise)

Preceding gene: 15673839

Following gene: 15673842

Centisome position: 81.49

GC content: 36.88

Gene sequence:

>1128_bases
ATTATATATTTAGATAACGCTGCTACAACACCTCTACTTCCTGATGTTATCACCGCAATCTCAGAAAGTTTATCCGCTAA
TTTTGGAAACCCCTCTAGCATTCACAGTCTCGGACGTGCTTCAAGTCAGATTGTTCGTAAAGCCCGTGAAACTGTGGCAA
ATGCTCTAGAAGTTCCAAGTCGCAATATCATTTTTACTTCTGGTGCTAGCGAAGCAAATAACCAAGCCTTAATTGGTTAT
GCACTGGCTAATAAAGATAAAGGAAATCATATCATCACAACAGCTATTGAACATCCTTCTGTTCTAAATACTGTTCAATA
TCTTCATGACCGTTATGGATTTGATGTTAGCTTTGTAAAACCAAAAGCGGACGGAACATTCCCAGCTTCACTTATAGAAG
AAAACTTGCGCCCTGATACAATTTTAGTCAGCATGATGTGGGCTAATAATGAAACAGGCCAACTTCTTCCAGTAAAGGAA
GTCGGAGAGCTCTTGAAGCAACATCAAGCCGTTTTTCATGTTGATGCTACTCAAGTAATGGGAAAAATTTCAGTGCATCC
TCTGGATGTTTCGGCTGATTTCCTCTCAGCATCAGCGCACAAATTTCATGGACCAAAAGGGGTCGGCTTCTTGTACTATA
ACGAAAAGCTACATTTTGATGCTTTGATTCATGGAGGAGAACAAGAAGAAAAAAGACGGGCTGGTACAGAAAATCTCCAT
TCTCTTGTTGGTTTAGCTAAAGCCTTAGAACTTGCCCTTGAAAGTATTGAATCAAATTATCAAGCAGTTGAAAAACTTAA
TGAAACTTTACTAACTAAACTAGAAGAACAAAAGATTTCATTTTATAAAAATAATTTTGGTCCAAGTATGCCTCATGTTC
TAAATCTTGCTTTTCCGAATGAAAATCATGATTTGCTCTTGACCAAACTTGATTTAGCTGGTTTCGCAATCTCAACTGGT
TCTGCTTGTACAGCCGGTACAATCGAACCTTCCCATGTTCTTGAAGCTGTTTACGGGAAAAATTCTGAAAAACTAAAAGA
AAACATACGTATTTCTTTTTCAGAAATGAATACAATTGATGAAGTTAATCTCTTCGTTGAAAAACTTAGTTCTATTTTAA
AAAAATAA

Upstream 100 bases:

>100_bases
TAAAATAGAGCTTACAGATAAGGGGACTTTTAATCCTGATGGAATTAAATCATTTCCTTATAAAATATCCAGCAATTACT
ATGAAAGGTAGGAAAATCAA

Downstream 100 bases:

>100_bases
ACAATTTATTTAGGAGACTCACAATGGCATTTATCACCGAAAAACATTTGGAAGGTAGCAAATATGTTTATCAACTTGCG
CCTACTGTTAAAAAATTCAC

Product: pyridoxal-phosphate dependent aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MIYLDNAATTPLLPDVITAISESLSANFGNPSSIHSLGRASSQIVRKARETVANALEVPSRNIIFTSGASEANNQALIGY
ALANKDKGNHIITTAIEHPSVLNTVQYLHDRYGFDVSFVKPKADGTFPASLIEENLRPDTILVSMMWANNETGQLLPVKE
VGELLKQHQAVFHVDATQVMGKISVHPLDVSADFLSASAHKFHGPKGVGFLYYNEKLHFDALIHGGEQEEKRRAGTENLH
SLVGLAKALELALESIESNYQAVEKLNETLLTKLEEQKISFYKNNFGPSMPHVLNLAFPNENHDLLLTKLDLAGFAISTG
SACTAGTIEPSHVLEAVYGKNSEKLKENIRISFSEMNTIDEVNLFVEKLSSILKK

Sequences:

>Translated_375_residues
MIYLDNAATTPLLPDVITAISESLSANFGNPSSIHSLGRASSQIVRKARETVANALEVPSRNIIFTSGASEANNQALIGY
ALANKDKGNHIITTAIEHPSVLNTVQYLHDRYGFDVSFVKPKADGTFPASLIEENLRPDTILVSMMWANNETGQLLPVKE
VGELLKQHQAVFHVDATQVMGKISVHPLDVSADFLSASAHKFHGPKGVGFLYYNEKLHFDALIHGGEQEEKRRAGTENLH
SLVGLAKALELALESIESNYQAVEKLNETLLTKLEEQKISFYKNNFGPSMPHVLNLAFPNENHDLLLTKLDLAGFAISTG
SACTAGTIEPSHVLEAVYGKNSEKLKENIRISFSEMNTIDEVNLFVEKLSSILKK
>Mature_375_residues
MIYLDNAATTPLLPDVITAISESLSANFGNPSSIHSLGRASSQIVRKARETVANALEVPSRNIIFTSGASEANNQALIGY
ALANKDKGNHIITTAIEHPSVLNTVQYLHDRYGFDVSFVKPKADGTFPASLIEENLRPDTILVSMMWANNETGQLLPVKE
VGELLKQHQAVFHVDATQVMGKISVHPLDVSADFLSASAHKFHGPKGVGFLYYNEKLHFDALIHGGEQEEKRRAGTENLH
SLVGLAKALELALESIESNYQAVEKLNETLLTKLEEQKISFYKNNFGPSMPHVLNLAFPNENHDLLLTKLDLAGFAISTG
SACTAGTIEPSHVLEAVYGKNSEKLKENIRISFSEMNTIDEVNLFVEKLSSILKK

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=379, Percent_Identity=36.1477572559367, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI156713448, Length=413, Percent_Identity=31.9612590799031, Blast_Score=169, Evalue=3e-42,
Organism=Escherichia coli, GI48994898, Length=372, Percent_Identity=35.752688172043, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI1787970, Length=401, Percent_Identity=25.9351620947631, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789175, Length=401, Percent_Identity=26.1845386533666, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25143064, Length=367, Percent_Identity=36.2397820163488, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17533177, Length=321, Percent_Identity=33.3333333333333, Blast_Score=134, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6319831, Length=377, Percent_Identity=34.4827586206897, Blast_Score=215, Evalue=1e-56,
Organism=Drosophila melanogaster, GI20129463, Length=375, Percent_Identity=37.0666666666667, Blast_Score=221, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41088; Mature: 41088

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDNAATTPLLPDVITAISESLSANFGNPSSIHSLGRASSQIVRKARETVANALEVPS
CEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RNIIFTSGASEANNQALIGYALANKDKGNHIITTAIEHPSVLNTVQYLHDRYGFDVSFVK
CCEEEECCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEC
PKADGTFPASLIEENLRPDTILVSMMWANNETGQLLPVKEVGELLKQHQAVFHVDATQVM
CCCCCCCCHHHHHCCCCCCEEEEEEEECCCCCCCEEEHHHHHHHHHHHHHEEEEEHHHHH
GKISVHPLDVSADFLSASAHKFHGPKGVGFLYYNEKLHFDALIHGGEQEEKRRAGTENLH
HHCEEEEECCCHHHHHCCHHHCCCCCCEEEEEECCEEEEEHEEECCCHHHHHHCCHHHHH
SLVGLAKALELALESIESNYQAVEKLNETLLTKLEEQKISFYKNNFGPSMPHVLNLAFPN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
ENHDLLLTKLDLAGFAISTGSACTAGTIEPSHVLEAVYGKNSEKLKENIRISFSEMNTID
CCCCEEEEEEEECCEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHEEEHHHHCCHH
EVNLFVEKLSSILKK
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MIYLDNAATTPLLPDVITAISESLSANFGNPSSIHSLGRASSQIVRKARETVANALEVPS
CEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RNIIFTSGASEANNQALIGYALANKDKGNHIITTAIEHPSVLNTVQYLHDRYGFDVSFVK
CCEEEECCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEC
PKADGTFPASLIEENLRPDTILVSMMWANNETGQLLPVKEVGELLKQHQAVFHVDATQVM
CCCCCCCCHHHHHCCCCCCEEEEEEEECCCCCCCEEEHHHHHHHHHHHHHEEEEEHHHHH
GKISVHPLDVSADFLSASAHKFHGPKGVGFLYYNEKLHFDALIHGGEQEEKRRAGTENLH
HHCEEEEECCCHHHHHCCHHHCCCCCCEEEEEECCEEEEEHEEECCCHHHHHHCCHHHHH
SLVGLAKALELALESIESNYQAVEKLNETLLTKLEEQKISFYKNNFGPSMPHVLNLAFPN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
ENHDLLLTKLDLAGFAISTGSACTAGTIEPSHVLEAVYGKNSEKLKENIRISFSEMNTID
CCCCEEEEEEEECCEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHEEEHHHHCCHH
EVNLFVEKLSSILKK
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]