Click here to switch to the map view.

The map label for this gene is proB

Identifier: 15673591

GI number: 15673591

Start: 1651877

End: 1652689

Strand: Reverse

Name: proB

Synonym: L0117

Alternate gene names: 15673591

Gene position: 1652689-1651877 (Counterclockwise)

Preceding gene: 15673592

Following gene: 15673590

Centisome position: 69.86

GC content: 35.67

Gene sequence:

>813_bases
ATGATTATGACAAGAAAAAATATTTTGAGTGTTAAACGAATTGTAGTTAAGATTGGAACGAGTTCGTTGATTTTGCCAAA
TGGAAAAATAAATTTATCAAATATAGACGAGTTGGCTTTTGTTTTATCTGATTTGAATAATAAAGGTTATGAAGTGATTT
TGGTGACTTCTGGAGCGATTGGAGTTGGCTTGAATGTGCTTGGTATAGATAAACGTCCCAAAGGGATTGCTGACCAGCAG
GCTTTGGCTTCAATCGGTCAGGTTGAATTGATGAGTCTTTATACGCAAATGTTTCGTCGTTATTCGCAAAAAGTTTCTCA
GTTACTTTTGACACGTGATGTGACTGATTTTCCAACGAGTCGTGAGAATGCTGAAAATGCACTAAATGCGTTACTTGGGC
TTGGGATTATTCCAATTATTAATGAAAATGATGCTATTGCGGTTGATGAAATGGATCATCAAACAAAATTTGGTGATAAT
GATAAATTGGGAGCGATTGTTTCTAAATTAGTGAATGCTGATTTATTGATTATGCTGTCAGATATCGATGGACTTTTTGA
TAAAAATCCATCGATTTATGATGATGCGAAAATTTTTAATGAAATTCATGAAATTACTGATGAATTACGTCAAATGGCTG
GTGGTGCCGGTTCGCGTTTTGGAACTGGCGGGATGACTTCAAAATTGGCGGCTGCACAGATTTTATTTGAAAATGGTCAA
GAAATGGTTCTGACCAATGGCGAACGGATTCGAGAGATTAAAGAAATTATCGAAGGACGTGAGATTGGGACTTATTTTCA
TCAAAAATCTTGA

Upstream 100 bases:

>100_bases
AGCACTTTTTTTATAAAATTCTAGTTGTAGAATAAAATAATAAAAAGTAGAAATTTAATCATTTTACTCTCGTATTTGTT
GAAATATCTTGCTATAATAA

Downstream 100 bases:

>100_bases
GAAAGAAGGATAAAACGATGATTGAAGAACTTGGATTAAAGGTTAAAAAGGCGAGCAAAGAAGTAGCAAAGCTATCAACT
GCCGACAAAAATGTTTTTTT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MIMTRKNILSVKRIVVKIGTSSLILPNGKINLSNIDELAFVLSDLNNKGYEVILVTSGAIGVGLNVLGIDKRPKGIADQQ
ALASIGQVELMSLYTQMFRRYSQKVSQLLLTRDVTDFPTSRENAENALNALLGLGIIPIINENDAIAVDEMDHQTKFGDN
DKLGAIVSKLVNADLLIMLSDIDGLFDKNPSIYDDAKIFNEIHEITDELRQMAGGAGSRFGTGGMTSKLAAAQILFENGQ
EMVLTNGERIREIKEIIEGREIGTYFHQKS

Sequences:

>Translated_270_residues
MIMTRKNILSVKRIVVKIGTSSLILPNGKINLSNIDELAFVLSDLNNKGYEVILVTSGAIGVGLNVLGIDKRPKGIADQQ
ALASIGQVELMSLYTQMFRRYSQKVSQLLLTRDVTDFPTSRENAENALNALLGLGIIPIINENDAIAVDEMDHQTKFGDN
DKLGAIVSKLVNADLLIMLSDIDGLFDKNPSIYDDAKIFNEIHEITDELRQMAGGAGSRFGTGGMTSKLAAAQILFENGQ
EMVLTNGERIREIKEIIEGREIGTYFHQKS
>Mature_270_residues
MIMTRKNILSVKRIVVKIGTSSLILPNGKINLSNIDELAFVLSDLNNKGYEVILVTSGAIGVGLNVLGIDKRPKGIADQQ
ALASIGQVELMSLYTQMFRRYSQKVSQLLLTRDVTDFPTSRENAENALNALLGLGIIPIINENDAIAVDEMDHQTKFGDN
DKLGAIVSKLVNADLLIMLSDIDGLFDKNPSIYDDAKIFNEIHEITDELRQMAGGAGSRFGTGGMTSKLAAAQILFENGQ
EMVLTNGERIREIKEIIEGREIGTYFHQKS

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate 5-kinase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=294, Percent_Identity=32.9931972789116, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI21361368, Length=296, Percent_Identity=32.7702702702703, Blast_Score=125, Evalue=4e-29,
Organism=Escherichia coli, GI1786437, Length=261, Percent_Identity=39.463601532567, Blast_Score=175, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17569969, Length=279, Percent_Identity=33.6917562724014, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17569967, Length=279, Percent_Identity=33.6917562724014, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320506, Length=249, Percent_Identity=39.7590361445783, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6321822, Length=248, Percent_Identity=37.0967741935484, Blast_Score=169, Evalue=5e-43,
Organism=Drosophila melanogaster, GI21357643, Length=296, Percent_Identity=33.445945945946, Blast_Score=141, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_LACLA (Q9CF72)

Other databases:

- EMBL:   AE005176
- PIR:   A86826
- RefSeq:   NP_267765.1
- ProteinModelPortal:   Q9CF72
- SMR:   Q9CF72
- GeneID:   1115269
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0117
- NMPDR:   fig|272623.1.peg.1651
- HOGENOM:   HBG507643
- OMA:   LINTIDP
- ProtClustDB:   PRK12314
- BioCyc:   LLAC272623:L0117-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.2.11

Molecular weight: Translated: 29612; Mature: 29612

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMTRKNILSVKRIVVKIGTSSLILPNGKINLSNIDELAFVLSDLNNKGYEVILVTSGAI
CCCCHHHHHHHHHHHHHCCCCEEEECCCCEECCHHHHHHHHHHHCCCCCEEEEEEECCCH
GVGLNVLGIDKRPKGIADQQALASIGQVELMSLYTQMFRRYSQKVSQLLLTRDVTDFPTS
HHCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RENAENALNALLGLGIIPIINENDAIAVDEMDHQTKFGDNDKLGAIVSKLVNADLLIMLS
CCHHHHHHHHHHHCCCEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEE
DIDGLFDKNPSIYDDAKIFNEIHEITDELRQMAGGAGSRFGTGGMTSKLAAAQILFENGQ
CCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCC
EMVLTNGERIREIKEIIEGREIGTYFHQKS
EEEEECCHHHHHHHHHHCCCHHHHHHCCCC
>Mature Secondary Structure
MIMTRKNILSVKRIVVKIGTSSLILPNGKINLSNIDELAFVLSDLNNKGYEVILVTSGAI
CCCCHHHHHHHHHHHHHCCCCEEEECCCCEECCHHHHHHHHHHHCCCCCEEEEEEECCCH
GVGLNVLGIDKRPKGIADQQALASIGQVELMSLYTQMFRRYSQKVSQLLLTRDVTDFPTS
HHCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RENAENALNALLGLGIIPIINENDAIAVDEMDHQTKFGDNDKLGAIVSKLVNADLLIMLS
CCHHHHHHHHHHHCCCEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEE
DIDGLFDKNPSIYDDAKIFNEIHEITDELRQMAGGAGSRFGTGGMTSKLAAAQILFENGQ
CCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCC
EMVLTNGERIREIKEIIEGREIGTYFHQKS
EEEEECCHHHHHHHHHHCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471