Click here to switch to the map view.

The map label for this gene is hmcM

Identifier: 15673554

GI number: 15673554

Start: 1613444

End: 1614598

Strand: Direct

Name: hmcM

Synonym: L13187

Alternate gene names: 15673554

Gene position: 1613444-1614598 (Clockwise)

Preceding gene: 15673535

Following gene: 15673557

Centisome position: 68.2

GC content: 36.19

Gene sequence:

>1155_bases
ATGAAAGTCGGTATAGATAAACTTGGATTTTTTGTCCCAAATACTTTTGTTGATATGAGGGATTTAGCAAATGCTCGTAA
TGTTGACCCTGCAAAATTCCAAATTGGTATTGGTCAAGATGAAATGGCAGTCAATCCTGCTACTCAAGATATTATTACTT
TTGCTGCTAACGCGGCAGCAAGTATTTTGACTGAGGAAGATAAAAAAGCCATTGACATGATCATCGTTGGTACTGAATCA
AGTCTTGATGAATCAAAGGCCTCAGCCGTTGTTGTTCACGATTTGCTTGGTATTCAACCTTTTGCTCGAAGCATTGAAAT
GAAAGAGGCTTGTTATGCAACTACTGCTGGCCTTACTCTTGCTCGTGACCACGTTCTTTTGAATCCTGATACTAAAGTTT
TAGTTATTGCTTCTGATATTGCCAAATATGGGCTAAATACTGGTGGAGAACCTACTCAGGGCGCTGGAAGTGTTGCAATG
CTTATTACGGCCGATCCTAAAATTTTGGCCTTAAATAATGATAACGTTGCTTTAACTCAAGACATCTATGATTTTTGGCG
TCCTTTTGGTCAGGCTTATCCTTCTGTTGATGGTAAGTTTTCAAACGAAACATATATCGATGCTTTTGCTAAAATCTGGA
AAGAGTATAGCCATAGAACAAATTTAAAGTTTGAAGACTTTGCAGCTATCGCCTTTCATACCCCTTATACTAAAATGGGT
AAAAAAGCTTTATTGCCAATGCTTGAATCTGAAAAACCAGCCAATGCTGAAGAATTAATGGAACAATTTGAACATGGTAT
CGTTTATAATCGTCGTGTGGGTAATCTGTATACCGGTTCTCTCTATTTGAGTTTGATTTCACTTTTGGAAAACTCTGATA
AATTGAGTGCTGGTCAGCGTATTGGACTTTTTAGTTATGGTTCTGGTACTGTTGCTGAGTTTTTCTCTGGTGAGCTTGTT
GAAGGTTATGAAAATCATTTACTTAAAATTGAACATCAATCAATGTTGGATGCAAGAACCCGTCTATCAATTCCTGAATA
CGAAAACATGTTTAATCAATATTTAGATTTGAATCATAATATTTCTTTCAATGATGAGACAGATTACTCCGTTTCAGAAG
TTGTTGATAATCACCGTAAGTATAAAAAAAGATAA

Upstream 100 bases:

>100_bases
CAAAGAATTTAACGTATTTTTATGATAAAATTAAAGCATTCCAAAGACATTGAAAGGAGTTCCTTTCTTTTATAATAATA
AGTGAGGAAATTTTTAAAAT

Downstream 100 bases:

>100_bases
AAAAATACTGACAGAATTTCTGTCAGTAAATAAAAAGTCCTGTCAGGACTTTTTTTTATTTTATTTGCTCAACAATGATA
CAGCTGCTTCATCAGCGATA

Product: hydroxymethylglutaryl-CoA synthase

Products: (S)-3-hydroxy-3-methylglutaryl-CoA; CoA

Alternate protein names: HMG synthase [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MKVGIDKLGFFVPNTFVDMRDLANARNVDPAKFQIGIGQDEMAVNPATQDIITFAANAAASILTEEDKKAIDMIIVGTES
SLDESKASAVVVHDLLGIQPFARSIEMKEACYATTAGLTLARDHVLLNPDTKVLVIASDIAKYGLNTGGEPTQGAGSVAM
LITADPKILALNNDNVALTQDIYDFWRPFGQAYPSVDGKFSNETYIDAFAKIWKEYSHRTNLKFEDFAAIAFHTPYTKMG
KKALLPMLESEKPANAEELMEQFEHGIVYNRRVGNLYTGSLYLSLISLLENSDKLSAGQRIGLFSYGSGTVAEFFSGELV
EGYENHLLKIEHQSMLDARTRLSIPEYENMFNQYLDLNHNISFNDETDYSVSEVVDNHRKYKKR

Sequences:

>Translated_384_residues
MKVGIDKLGFFVPNTFVDMRDLANARNVDPAKFQIGIGQDEMAVNPATQDIITFAANAAASILTEEDKKAIDMIIVGTES
SLDESKASAVVVHDLLGIQPFARSIEMKEACYATTAGLTLARDHVLLNPDTKVLVIASDIAKYGLNTGGEPTQGAGSVAM
LITADPKILALNNDNVALTQDIYDFWRPFGQAYPSVDGKFSNETYIDAFAKIWKEYSHRTNLKFEDFAAIAFHTPYTKMG
KKALLPMLESEKPANAEELMEQFEHGIVYNRRVGNLYTGSLYLSLISLLENSDKLSAGQRIGLFSYGSGTVAEFFSGELV
EGYENHLLKIEHQSMLDARTRLSIPEYENMFNQYLDLNHNISFNDETDYSVSEVVDNHRKYKKR
>Mature_384_residues
MKVGIDKLGFFVPNTFVDMRDLANARNVDPAKFQIGIGQDEMAVNPATQDIITFAANAAASILTEEDKKAIDMIIVGTES
SLDESKASAVVVHDLLGIQPFARSIEMKEACYATTAGLTLARDHVLLNPDTKVLVIASDIAKYGLNTGGEPTQGAGSVAM
LITADPKILALNNDNVALTQDIYDFWRPFGQAYPSVDGKFSNETYIDAFAKIWKEYSHRTNLKFEDFAAIAFHTPYTKMG
KKALLPMLESEKPANAEELMEQFEHGIVYNRRVGNLYTGSLYLSLISLLENSDKLSAGQRIGLFSYGSGTVAEFFSGELV
EGYENHLLKIEHQSMLDARTRLSIPEYENMFNQYLDLNHNISFNDETDYSVSEVVDNHRKYKKR

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the aldol condensation between the acetyl group attached to the acyl-carrier-protein AcpK (Ac- AcpK) and

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA synthase family [H]

Homologues:

Organism=Homo sapiens, GI5031751, Length=454, Percent_Identity=26.6519823788546, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI148298764, Length=402, Percent_Identity=28.1094527363184, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI148298677, Length=402, Percent_Identity=28.1094527363184, Blast_Score=129, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17560126, Length=392, Percent_Identity=26.530612244898, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323509, Length=402, Percent_Identity=27.363184079602, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24654139, Length=421, Percent_Identity=26.603325415677, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24654137, Length=421, Percent_Identity=26.603325415677, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24654133, Length=421, Percent_Identity=26.603325415677, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24654135, Length=421, Percent_Identity=26.603325415677, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI17933694, Length=421, Percent_Identity=26.603325415677, Blast_Score=132, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013746
- InterPro:   IPR013528
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08540 HMG_CoA_synt_C; PF01154 HMG_CoA_synt_N [H]

EC number: 2.3.3.10

Molecular weight: Translated: 42715; Mature: 42715

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGIDKLGFFVPNTFVDMRDLANARNVDPAKFQIGIGQDEMAVNPATQDIITFAANAAA
CCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
SILTEEDKKAIDMIIVGTESSLDESKASAVVVHDLLGIQPFARSIEMKEACYATTAGLTL
HHHHHCCCCEEEEEEECCCCCCCHHHHHEEHHHHHHCCCHHHHHHHHHHHHHHHHCCCEE
ARDHVLLNPDTKVLVIASDIAKYGLNTGGEPTQGAGSVAMLITADPKILALNNDNVALTQ
ECCEEEECCCCEEEEEECHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCEEEHH
DIYDFWRPFGQAYPSVDGKFSNETYIDAFAKIWKEYSHRTNLKFEDFAAIAFHTPYTKMG
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCHHHHH
KKALLPMLESEKPANAEELMEQFEHGIVYNRRVGNLYTGSLYLSLISLLENSDKLSAGQR
HHHHHHHHCCCCCCCHHHHHHHHHCCCEEEECCCCEEHHHHHHHHHHHHCCCCCCCCCCE
IGLFSYGSGTVAEFFSGELVEGYENHLLKIEHQSMLDARTRLSIPEYENMFNQYLDLNHN
EEEEECCCCHHHHHHCCHHHHCHHHCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
ISFNDETDYSVSEVVDNHRKYKKR
CCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVGIDKLGFFVPNTFVDMRDLANARNVDPAKFQIGIGQDEMAVNPATQDIITFAANAAA
CCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
SILTEEDKKAIDMIIVGTESSLDESKASAVVVHDLLGIQPFARSIEMKEACYATTAGLTL
HHHHHCCCCEEEEEEECCCCCCCHHHHHEEHHHHHHCCCHHHHHHHHHHHHHHHHCCCEE
ARDHVLLNPDTKVLVIASDIAKYGLNTGGEPTQGAGSVAMLITADPKILALNNDNVALTQ
ECCEEEECCCCEEEEEECHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCEEEHH
DIYDFWRPFGQAYPSVDGKFSNETYIDAFAKIWKEYSHRTNLKFEDFAAIAFHTPYTKMG
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCHHHHH
KKALLPMLESEKPANAEELMEQFEHGIVYNRRVGNLYTGSLYLSLISLLENSDKLSAGQR
HHHHHHHHCCCCCCCHHHHHHHHHCCCEEEECCCCEEHHHHHHHHHHHHCCCCCCCCCCE
IGLFSYGSGTVAEFFSGELVEGYENHLLKIEHQSMLDARTRLSIPEYENMFNQYLDLNHN
EEEEECCCCHHHHHHCCHHHHCHHHCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
ISFNDETDYSVSEVVDNHRKYKKR
CCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; H2O; acetoacetyl-CoA

Specific reaction: acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]