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The map label for this gene is rlrA

Identifier: 15673218

GI number: 15673218

Start: 1263405

End: 1264316

Strand: Direct

Name: rlrA

Synonym: L0214

Alternate gene names: 15673218

Gene position: 1263405-1264316 (Clockwise)

Preceding gene: 15673217

Following gene: 15673225

Centisome position: 53.41

GC content: 28.73

Gene sequence:

>912_bases
ATGAAATTATCAACTCTACGATATTTTGTTGAGGTAGCAACTGAGAACAGCTTCACAAAAGCTTCACAAAATTTATTTAT
CTCTCAACCAACTTTAAGCAGAAGGATTCAAGAATTAGAGTCAGAGTTAGGGGTAACACTCTTTAACCGCAATAACACAA
ATAATATAATCCTTTCCCCAGAAGGTAAACAATTACTGGTGAAAGTTAGAGATGTTTTAGATCAAATTGAATCTATATCA
GGGATGTTTGATCCTGAAGGGCCTAACAAGAACAATAAGGCATCAATTAAAATTGGATGTTTAACCAATTTTGACTTTAG
TAAATTGTACGATTTAATAGAGCAGTTTAAAACACAATACCCAAACGTAAAATTTATAATTGAACAAGGTTCTCCCATGG
AGCTAAATGATGGATTATTGAACAGGACGTATGATTTGGTACTTAATTTAGATAATTATATTTCTAATAATGATGTGCTC
AAAACTTCTTTATATAAAAATAATAATTTACAAGTAGCCATTCAAAATAATCACAGATTAGCATCAAAACAAGAAATACA
ATTTTCTGATTTAACTGATGAAACACTTATTCTCATTGAAAGAAATAAATCCCCTCTGGTATTTGATTATGTAATCAATC
AATGTTTGAAACATGGTTTTCATGCAAAAGCTAATTATTATGTTAAGAATTTAGATGAGGGACTTTCTAAAACATCACTT
GGAGAAGGGATTTCATTTCTTTACTCAGGCATGGATAATGGTTATTTATCAAAAAAATATCGTGTAAAATTTTTAAATTT
GAACGAAGAGAAAAGTTCACAAAATTTGGTTTATGCTTTTCAAGAAAATAATAATCCTATGTTGGATGAATTTTTAAACT
TCGTTCATCAAAATAGATCTGATTTATATTAA

Upstream 100 bases:

>100_bases
ACTTCTTATCTTCAACTTCCTCTTCTAAACTAATTATTCTGCCTTTTACCATATGACTTTAGGGATCATTTATGATATTC
TAGTAATGATCGGAGGCCAG

Downstream 100 bases:

>100_bases
AAACTCATCTTGCTATTCATTGCAAGATGAGTTTTTGACTGACCATAATTATTTTATATGTTATTAATACCAATCCGCTT
TGTACAAGCTTTTTTTATAT

Product: LysR family transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKLSTLRYFVEVATENSFTKASQNLFISQPTLSRRIQELESELGVTLFNRNNTNNIILSPEGKQLLVKVRDVLDQIESIS
GMFDPEGPNKNNKASIKIGCLTNFDFSKLYDLIEQFKTQYPNVKFIIEQGSPMELNDGLLNRTYDLVLNLDNYISNNDVL
KTSLYKNNNLQVAIQNNHRLASKQEIQFSDLTDETLILIERNKSPLVFDYVINQCLKHGFHAKANYYVKNLDEGLSKTSL
GEGISFLYSGMDNGYLSKKYRVKFLNLNEEKSSQNLVYAFQENNNPMLDEFLNFVHQNRSDLY

Sequences:

>Translated_303_residues
MKLSTLRYFVEVATENSFTKASQNLFISQPTLSRRIQELESELGVTLFNRNNTNNIILSPEGKQLLVKVRDVLDQIESIS
GMFDPEGPNKNNKASIKIGCLTNFDFSKLYDLIEQFKTQYPNVKFIIEQGSPMELNDGLLNRTYDLVLNLDNYISNNDVL
KTSLYKNNNLQVAIQNNHRLASKQEIQFSDLTDETLILIERNKSPLVFDYVINQCLKHGFHAKANYYVKNLDEGLSKTSL
GEGISFLYSGMDNGYLSKKYRVKFLNLNEEKSSQNLVYAFQENNNPMLDEFLNFVHQNRSDLY
>Mature_303_residues
MKLSTLRYFVEVATENSFTKASQNLFISQPTLSRRIQELESELGVTLFNRNNTNNIILSPEGKQLLVKVRDVLDQIESIS
GMFDPEGPNKNNKASIKIGCLTNFDFSKLYDLIEQFKTQYPNVKFIIEQGSPMELNDGLLNRTYDLVLNLDNYISNNDVL
KTSLYKNNNLQVAIQNNHRLASKQEIQFSDLTDETLILIERNKSPLVFDYVINQCLKHGFHAKANYYVKNLDEGLSKTSL
GEGISFLYSGMDNGYLSKKYRVKFLNLNEEKSSQNLVYAFQENNNPMLDEFLNFVHQNRSDLY

Specific function: Positive regulator of glutamate biosynthesis (gltAB genes). Negatively regulates its own expression [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693105, Length=247, Percent_Identity=28.7449392712551, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1787530, Length=198, Percent_Identity=26.7676767676768, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787879, Length=294, Percent_Identity=23.8095238095238, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI2367136, Length=255, Percent_Identity=27.4509803921569, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790399, Length=249, Percent_Identity=23.2931726907631, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI157672245, Length=220, Percent_Identity=25.4545454545455, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789173, Length=126, Percent_Identity=26.984126984127, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790208, Length=148, Percent_Identity=27.027027027027, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786713, Length=181, Percent_Identity=27.0718232044199, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34907; Mature: 34907

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSTLRYFVEVATENSFTKASQNLFISQPTLSRRIQELESELGVTLFNRNNTNNIILSP
CCCHHHHHHHHHHCCCCHHHHCCCEEEECCHHHHHHHHHHHHCCEEEEECCCCCEEEECC
EGKQLLVKVRDVLDQIESISGMFDPEGPNKNNKASIKIGCLTNFDFSKLYDLIEQFKTQY
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHC
PNVKFIIEQGSPMELNDGLLNRTYDLVLNLDNYISNNDVLKTSLYKNNNLQVAIQNNHRL
CCEEEEEECCCCCCCCCCHHHCEEEEEEEHHHHCCCCCEEEEEEEECCCEEEEECCCCCC
ASKQEIQFSDLTDETLILIERNKSPLVFDYVINQCLKHGFHAKANYYVKNLDEGLSKTSL
CCCCCCEECCCCCCEEEEEECCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
GEGISFLYSGMDNGYLSKKYRVKFLNLNEEKSSQNLVYAFQENNNPMLDEFLNFVHQNRS
HHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCC
DLY
CCC
>Mature Secondary Structure
MKLSTLRYFVEVATENSFTKASQNLFISQPTLSRRIQELESELGVTLFNRNNTNNIILSP
CCCHHHHHHHHHHCCCCHHHHCCCEEEECCHHHHHHHHHHHHCCEEEEECCCCCEEEECC
EGKQLLVKVRDVLDQIESISGMFDPEGPNKNNKASIKIGCLTNFDFSKLYDLIEQFKTQY
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHC
PNVKFIIEQGSPMELNDGLLNRTYDLVLNLDNYISNNDVLKTSLYKNNNLQVAIQNNHRL
CCEEEEEECCCCCCCCCCHHHCEEEEEEEHHHHCCCCCEEEEEEEECCCEEEEECCCCCC
ASKQEIQFSDLTDETLILIERNKSPLVFDYVINQCLKHGFHAKANYYVKNLDEGLSKTSL
CCCCCCEECCCCCCEEEEEECCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
GEGISFLYSGMDNGYLSKKYRVKFLNLNEEKSSQNLVYAFQENNNPMLDEFLNFVHQNRS
HHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCC
DLY
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2548995; 9384377 [H]