BacMap

Definition	Lactococcus lactis subsp. lactis Il1403, complete genome.
Accession	NC_002662
Length	2,365,589

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The map label for this gene is ymgK

Identifier: 15673225

GI number: 15673225

Start: 1267992

End: 1268834

Strand: Direct

Name: ymgK

Synonym: L67726

Alternate gene names: 15673225

Gene position: 1267992-1268834 (Clockwise)

Preceding gene: 15673218

Following gene: 15673233

Centisome position: 53.6

GC content: 35.35

Gene sequence:

>843_bases
ATGATTGAACAAGAAAAATATACGCTTAATGATGGACATTTGATTCCTAAAATAGGGTTAGGAACATTTCAGATTCGTGG
ATATGAAGGGGTGGAGCAAATTTTAACGGCTATACAAAGCGGTTATCGTTTACTTGATACCTCAACTAATTATGATAGCG
AAGGAGCAGTTGGAGAGGCAATTCGTCGTTCTGGCATCCCTCGATCAAATTTTTTTGTGACAACTAAGCTACCTGGTAAA
TATCATCATTTTGAAGATGCTTTGAAGATGATTGAAGAGTCTTTATTACGAACAGGATTGAGCTATTTTGATCTTTACTT
GATCCACTGGCCACTACCAAAGCGAGATAATTATGTTGAAGCTTGGAAAGCACTAATTGCAGCAAAAAAAAGGGGTCTTG
TAAGATCAATTGGGGTATCTAATTTTGAGGAAGACCATCTCGACAAAATAATTCAAGAAACTGGTGTTATACCAGCAATT
AATCAAAATGAGATTCACCCTTACTGGCCTCAAGAAAAGCTATTAAAAATTAATAAAAAGTATGGCATTTTAACTGAGGC
ATGGAGCCCTTTAGGCCGAGGTAGTAAAGAGCTTGAGGAAAGCCTGATTCAAAACATAGCTCAAAAATATGGTAAGGATA
CTGGGCAAATCATTCTAAGATGGCAAGTTGAAAGAGGTATTATACCTATTGTTAAAGCAACTTCTCCAGAACACCAAAGA
AGAAATATAGATATTTTTGATTTTTCACTTTCTTCTGAAGAGATTAATAAAATCACAGCCTTAAAACAAGAAAATGGCAG
GGTAGATAATCAAGATCCTAATGAGTATGAAGAATTTGTGTAA

Upstream 100 bases:

>100_bases
GATTAGATATTCGATTAACTTTGAAAACCTGCAAAAGTTTAGTATTATTGACATAATATTCATAAACTTAAACAATCAGA
AAGGAAAATAGAACAAGAAA

Downstream 100 bases:

>100_bases
TTGTATTACTCTGCTCTTGAATAATTAATTTCAATTGAATATATGAAGAATTGTTTCAAAAAAAACCACTAAAATTTTCA
AGTGGTTTTTTAGCTTCTAT

Product: oxidoreductase

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MIEQEKYTLNDGHLIPKIGLGTFQIRGYEGVEQILTAIQSGYRLLDTSTNYDSEGAVGEAIRRSGIPRSNFFVTTKLPGK
YHHFEDALKMIEESLLRTGLSYFDLYLIHWPLPKRDNYVEAWKALIAAKKRGLVRSIGVSNFEEDHLDKIIQETGVIPAI
NQNEIHPYWPQEKLLKINKKYGILTEAWSPLGRGSKELEESLIQNIAQKYGKDTGQIILRWQVERGIIPIVKATSPEHQR
RNIDIFDFSLSSEEINKITALKQENGRVDNQDPNEYEEFV

Sequences:

>Translated_280_residues
MIEQEKYTLNDGHLIPKIGLGTFQIRGYEGVEQILTAIQSGYRLLDTSTNYDSEGAVGEAIRRSGIPRSNFFVTTKLPGK
YHHFEDALKMIEESLLRTGLSYFDLYLIHWPLPKRDNYVEAWKALIAAKKRGLVRSIGVSNFEEDHLDKIIQETGVIPAI
NQNEIHPYWPQEKLLKINKKYGILTEAWSPLGRGSKELEESLIQNIAQKYGKDTGQIILRWQVERGIIPIVKATSPEHQR
RNIDIFDFSLSSEEINKITALKQENGRVDNQDPNEYEEFV
>Mature_280_residues
MIEQEKYTLNDGHLIPKIGLGTFQIRGYEGVEQILTAIQSGYRLLDTSTNYDSEGAVGEAIRRSGIPRSNFFVTTKLPGK
YHHFEDALKMIEESLLRTGLSYFDLYLIHWPLPKRDNYVEAWKALIAAKKRGLVRSIGVSNFEEDHLDKIIQETGVIPAI
NQNEIHPYWPQEKLLKINKKYGILTEAWSPLGRGSKELEESLIQNIAQKYGKDTGQIILRWQVERGIIPIVKATSPEHQR
RNIDIFDFSLSSEEINKITALKQENGRVDNQDPNEYEEFV

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). 25DKGR-B has higher catalytic efficiency than 25DKGR-A [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=287, Percent_Identity=35.5400696864112, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI5174391, Length=287, Percent_Identity=35.5400696864112, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI5453543, Length=292, Percent_Identity=33.5616438356164, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI310109920, Length=292, Percent_Identity=33.5616438356164, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI45446745, Length=292, Percent_Identity=33.2191780821918, Blast_Score=158, Evalue=5e-39,
Organism=Homo sapiens, GI4503285, Length=292, Percent_Identity=33.2191780821918, Blast_Score=158, Evalue=5e-39,
Organism=Homo sapiens, GI310109922, Length=274, Percent_Identity=34.6715328467153, Blast_Score=157, Evalue=7e-39,
Organism=Homo sapiens, GI223468663, Length=289, Percent_Identity=33.5640138408304, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI5174695, Length=294, Percent_Identity=34.3537414965986, Blast_Score=154, Evalue=8e-38,
Organism=Homo sapiens, GI24497583, Length=290, Percent_Identity=32.0689655172414, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI291291012, Length=281, Percent_Identity=31.6725978647687, Blast_Score=147, Evalue=8e-36,
Organism=Homo sapiens, GI24497585, Length=296, Percent_Identity=31.7567567567568, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI93277124, Length=296, Percent_Identity=29.3918918918919, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI4502049, Length=293, Percent_Identity=33.1058020477816, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI300116273, Length=276, Percent_Identity=32.6086956521739, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI300116271, Length=274, Percent_Identity=31.3868613138686, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI310109924, Length=148, Percent_Identity=35.1351351351351, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI310109926, Length=123, Percent_Identity=36.5853658536585, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI207028673, Length=123, Percent_Identity=35.7723577235772, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI87082198, Length=269, Percent_Identity=40.5204460966543, Blast_Score=221, Evalue=4e-59,
Organism=Escherichia coli, GI1786400, Length=249, Percent_Identity=36.144578313253, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1788081, Length=266, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17537077, Length=293, Percent_Identity=36.1774744027304, Blast_Score=165, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17564128, Length=291, Percent_Identity=33.3333333333333, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17537075, Length=287, Percent_Identity=36.2369337979094, Blast_Score=161, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17566692, Length=290, Percent_Identity=36.2068965517241, Blast_Score=157, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17552492, Length=280, Percent_Identity=33.5714285714286, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71998625, Length=260, Percent_Identity=31.9230769230769, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17561298, Length=268, Percent_Identity=32.089552238806, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17537079, Length=291, Percent_Identity=29.553264604811, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17561300, Length=273, Percent_Identity=34.4322344322344, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17550248, Length=287, Percent_Identity=31.3588850174216, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17538386, Length=281, Percent_Identity=30.2491103202847, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17562292, Length=274, Percent_Identity=29.9270072992701, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6324694, Length=303, Percent_Identity=36.3036303630363, Blast_Score=184, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6320576, Length=287, Percent_Identity=33.4494773519164, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322556, Length=258, Percent_Identity=32.5581395348837, Blast_Score=141, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6321896, Length=313, Percent_Identity=30.6709265175719, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6320079, Length=287, Percent_Identity=30.6620209059233, Blast_Score=135, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6319625, Length=290, Percent_Identity=32.4137931034483, Blast_Score=129, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24657054, Length=275, Percent_Identity=37.8181818181818, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24663317, Length=277, Percent_Identity=35.3790613718412, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24644950, Length=302, Percent_Identity=31.7880794701987, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI21356425, Length=275, Percent_Identity=34.1818181818182, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI20129731, Length=272, Percent_Identity=32.3529411764706, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI221378297, Length=322, Percent_Identity=29.5031055900621, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI45553081, Length=276, Percent_Identity=32.2463768115942, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24662789, Length=300, Percent_Identity=29.6666666666667, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24662785, Length=294, Percent_Identity=35.0340136054422, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24662781, Length=291, Percent_Identity=34.3642611683849, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI281366140, Length=244, Percent_Identity=32.7868852459016, Blast_Score=128, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 32157; Mature: 32157

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEQEKYTLNDGHLIPKIGLGTFQIRGYEGVEQILTAIQSGYRLLDTSTNYDSEGAVGEA
CCCCCCEECCCCCEEEEECCCEEEECCHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHH
IRRSGIPRSNFFVTTKLPGKYHHFEDALKMIEESLLRTGLSYFDLYLIHWPLPKRDNYVE
HHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCHHHHEEEEEECCCCCCCCHHH
AWKALIAAKKRGLVRSIGVSNFEEDHLDKIIQETGVIPAINQNEIHPYWPQEKLLKINKK
HHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
YGILTEAWSPLGRGSKELEESLIQNIAQKYGKDTGQIILRWQVERGIIPIVKATSPEHQR
CCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEECCCCHHHH
RNIDIFDFSLSSEEINKITALKQENGRVDNQDPNEYEEFV
CCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCC
>Mature Secondary Structure
MIEQEKYTLNDGHLIPKIGLGTFQIRGYEGVEQILTAIQSGYRLLDTSTNYDSEGAVGEA
CCCCCCEECCCCCEEEEECCCEEEECCHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHH
IRRSGIPRSNFFVTTKLPGKYHHFEDALKMIEESLLRTGLSYFDLYLIHWPLPKRDNYVE
HHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCHHHHEEEEEECCCCCCCCHHH
AWKALIAAKKRGLVRSIGVSNFEEDHLDKIIQETGVIPAINQNEIHPYWPQEKLLKINKK
HHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
YGILTEAWSPLGRGSKELEESLIQNIAQKYGKDTGQIILRWQVERGIIPIVKATSPEHQR
CCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEECCCCHHHH
RNIDIFDFSLSSEEINKITALKQENGRVDNQDPNEYEEFV
CCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA