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The map label for this gene is ilvD

Identifier: 15673205

GI number: 15673205

Start: 1245771

End: 1247483

Strand: Direct

Name: ilvD

Synonym: L0077

Alternate gene names: 15673205

Gene position: 1245771-1247483 (Clockwise)

Preceding gene: 30024028

Following gene: 15673206

Centisome position: 52.66

GC content: 40.4

Gene sequence:

>1713_bases
ATGGAATTCAAATATAACGGAAAAGTTGAATCAGTGGAACTCAATAAATATTCTAAGACATTGACTCAAGATCCAACACA
ACCAGCGACTCAAGCGATGTACTACGGCATTGGTTTTAAAGATGAGGATTTCAAAAAAGCTCAGGTCGGAATCGTCAGCA
TGGATTGGGACGGAAATCCATGTAATATGCACTTGGGAACACTTGGGAGTAAAATCAAAAGTTCTGTCAACCAAACTGAC
GGATTGATTGGACTTCAATTTCATACTATTGGAGTTTCTGATGGAATTGCTAACGGAAAGCTTGGCATGAGATATTCTTT
GGTCAGTCGTGAAGTGATTGCTGACAGCATCGAAACCAACGCTGGCGCAGAATATTATGATGCCATCGTTGCCATTCCCG
GTTGTGATAAAAATATGCCAGGGTCAATTATCGGAATGGCTCGCTTAAATCGTCCGTCAATTATGGTCTATGGTGGAACG
ATTGAACATGGCGAATATAAAGGTGAAAAATTAAATATTGTTTCGGCCTTTGAATCTCTGGGACAAAAAATCACTGGAAA
TATTTCTGATGAAGATTATCATGGCGTTATTTGCAATGCCATTCCAGGGCAAGGTGCTTGCGGAGGAATGTACACTGCTA
ATACCCTGGCTGCCGCTATTGAAACTTTAGGAATGAGTTTACCTTATTCCTCTTCCAATCCAGCAGTCAGTCAAGAAAAA
CAAGAAGAGTGTGATGAAATTGGTTTAGCCATCAAAAATTTATTAGAAAAAGATATTAAACCAAGTGATATCATGACCAA
AGAAGCTTTTGAAAATGCCATAACAATTGTCATGGTCCTTGGAGGCTCAACCAATGCTGTGCTTCATATCATTGCAATGG
CAAATGCCATAGGTGTAGAAATTACGCAAGATGATTTCCAACGTATTTCAGATATTACCCCTGTTCTTGGCGATTTCAAA
CCGAGCGGAAAATATATGATGGAAGATCTGCACAAAATTGGTGGCCTTCCTGCTGTTTTGAAATACCTACTTAAAGAAGG
AAAACTTCACGGTGATTGTTTGACCGTCACAGGTAAAACTTTGGCTGAAAATGTTGAAACAGCATTAGATTTGGACTTTG
ACAGCCAAGATATTATGCGACCACTAAAAAATCCAATTAAAGCTACTGGACATTTACAAATCTTGTACGGTAATCTTGCC
CAAGGGGGTTCTGTTGCAAAAATTTCTGGTAAAGAAGGCGAATTTTTCAAAGGAACTGCTCGTGTTTTTGACGGAGAACA
ACACTTTATCGATGGCATTGAGTCTGGCCGATTGCATGCCGGTGATGTTGCGGTCATTAGAAATATTGGCCCAGTCGGAG
GTCCGGGAATGCCAGAGATGTTAAAACCAACCTCAGCATTAATTGGAGCAGGACTTGGAAAATCTTGTGCCCTAATTACT
GACGGAAGATTTTCTGGCGGCACACACGGCTTTGTTGTGGGTCATATCGTCCCTGAAGCAGTTGAAGGTGGGTTGATTGG
TTTAGTTGAAGATGATGATATTATCGAAATTGATGCGGTGAATAATAGTATTAGTTTAAAAGTTTCTGATGAAGAAATTG
CTAAACGACGTGCCAATTATCAAAAACCAACCCCTAAAGCAACGCGTGGTGTCCTTGCAAAATTTGCCAAACTTACACGC
CCCGCTAGTGAAGGTTGCGTTACAGATTTATAG

Upstream 100 bases:

>100_bases
TATAGACTATATAGAATATTCTGACAAATTATTGTATTTTCATTTTTTTAGTGATAAAATAGCTCTATGTAAATTTACGG
GGAGGTCAAAAAGATAACAT

Downstream 100 bases:

>100_bases
AAAGGTTTGAATGAAAAAAATATAGTTAGAAAAACCTACTTCCGGTTCCCAACTTGTTCTCCAAACCTTAAAAGAACTTG
GAGTAGAAATTATTTTTGGT

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MEFKYNGKVESVELNKYSKTLTQDPTQPATQAMYYGIGFKDEDFKKAQVGIVSMDWDGNPCNMHLGTLGSKIKSSVNQTD
GLIGLQFHTIGVSDGIANGKLGMRYSLVSREVIADSIETNAGAEYYDAIVAIPGCDKNMPGSIIGMARLNRPSIMVYGGT
IEHGEYKGEKLNIVSAFESLGQKITGNISDEDYHGVICNAIPGQGACGGMYTANTLAAAIETLGMSLPYSSSNPAVSQEK
QEECDEIGLAIKNLLEKDIKPSDIMTKEAFENAITIVMVLGGSTNAVLHIIAMANAIGVEITQDDFQRISDITPVLGDFK
PSGKYMMEDLHKIGGLPAVLKYLLKEGKLHGDCLTVTGKTLAENVETALDLDFDSQDIMRPLKNPIKATGHLQILYGNLA
QGGSVAKISGKEGEFFKGTARVFDGEQHFIDGIESGRLHAGDVAVIRNIGPVGGPGMPEMLKPTSALIGAGLGKSCALIT
DGRFSGGTHGFVVGHIVPEAVEGGLIGLVEDDDIIEIDAVNNSISLKVSDEEIAKRRANYQKPTPKATRGVLAKFAKLTR
PASEGCVTDL

Sequences:

>Translated_570_residues
MEFKYNGKVESVELNKYSKTLTQDPTQPATQAMYYGIGFKDEDFKKAQVGIVSMDWDGNPCNMHLGTLGSKIKSSVNQTD
GLIGLQFHTIGVSDGIANGKLGMRYSLVSREVIADSIETNAGAEYYDAIVAIPGCDKNMPGSIIGMARLNRPSIMVYGGT
IEHGEYKGEKLNIVSAFESLGQKITGNISDEDYHGVICNAIPGQGACGGMYTANTLAAAIETLGMSLPYSSSNPAVSQEK
QEECDEIGLAIKNLLEKDIKPSDIMTKEAFENAITIVMVLGGSTNAVLHIIAMANAIGVEITQDDFQRISDITPVLGDFK
PSGKYMMEDLHKIGGLPAVLKYLLKEGKLHGDCLTVTGKTLAENVETALDLDFDSQDIMRPLKNPIKATGHLQILYGNLA
QGGSVAKISGKEGEFFKGTARVFDGEQHFIDGIESGRLHAGDVAVIRNIGPVGGPGMPEMLKPTSALIGAGLGKSCALIT
DGRFSGGTHGFVVGHIVPEAVEGGLIGLVEDDDIIEIDAVNNSISLKVSDEEIAKRRANYQKPTPKATRGVLAKFAKLTR
PASEGCVTDL
>Mature_570_residues
MEFKYNGKVESVELNKYSKTLTQDPTQPATQAMYYGIGFKDEDFKKAQVGIVSMDWDGNPCNMHLGTLGSKIKSSVNQTD
GLIGLQFHTIGVSDGIANGKLGMRYSLVSREVIADSIETNAGAEYYDAIVAIPGCDKNMPGSIIGMARLNRPSIMVYGGT
IEHGEYKGEKLNIVSAFESLGQKITGNISDEDYHGVICNAIPGQGACGGMYTANTLAAAIETLGMSLPYSSSNPAVSQEK
QEECDEIGLAIKNLLEKDIKPSDIMTKEAFENAITIVMVLGGSTNAVLHIIAMANAIGVEITQDDFQRISDITPVLGDFK
PSGKYMMEDLHKIGGLPAVLKYLLKEGKLHGDCLTVTGKTLAENVETALDLDFDSQDIMRPLKNPIKATGHLQILYGNLA
QGGSVAKISGKEGEFFKGTARVFDGEQHFIDGIESGRLHAGDVAVIRNIGPVGGPGMPEMLKPTSALIGAGLGKSCALIT
DGRFSGGTHGFVVGHIVPEAVEGGLIGLVEDDDIIEIDAVNNSISLKVSDEEIAKRRANYQKPTPKATRGVLAKFAKLTR
PASEGCVTDL

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=614, Percent_Identity=36.9706840390879, Blast_Score=341, Evalue=8e-95,
Organism=Escherichia coli, GI2367371, Length=460, Percent_Identity=33.4782608695652, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1788157, Length=472, Percent_Identity=30.9322033898305, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1786464, Length=493, Percent_Identity=31.237322515213, Blast_Score=172, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6322476, Length=565, Percent_Identity=58.4070796460177, Blast_Score=650, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_LACLA (Q02139)

Other databases:

- EMBL:   U92974
- EMBL:   AE005176
- PIR:   G86777
- PIR:   S35137
- RefSeq:   NP_267379.1
- ProteinModelPortal:   Q02139
- SMR:   Q02139
- GeneID:   1114872
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0077
- NMPDR:   fig|272623.1.peg.1258
- HOGENOM:   HBG671001
- OMA:   IAMANAI
- ProtClustDB:   PRK00911
- BioCyc:   LLAC272623:L0077-MONOMER
- BRENDA:   4.2.1.9
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 60738; Mature: 60738

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKYNGKVESVELNKYSKTLTQDPTQPATQAMYYGIGFKDEDFKKAQVGIVSMDWDGNP
CCEEECCEEEEEECCHHHHHHCCCCCCHHHHHEEEECCCCCCCCCEEEEEEEEECCCCCC
CNMHLGTLGSKIKSSVNQTDGLIGLQFHTIGVSDGIANGKLGMRYSLVSREVIADSIETN
CEEEHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHCCC
AGAEYYDAIVAIPGCDKNMPGSIIGMARLNRPSIMVYGGTIEHGEYKGEKLNIVSAFESL
CCCCEEEEEEECCCCCCCCCCCEEEEEECCCCEEEEECCEEECCCCCCCEEHHHHHHHHH
GQKITGNISDEDYHGVICNAIPGQGACGGMYTANTLAAAIETLGMSLPYSSSNPAVSQEK
CHHEECCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
QEECDEIGLAIKNLLEKDIKPSDIMTKEAFENAITIVMVLGGSTNAVLHIIAMANAIGVE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEEEECCCCHHHEEHHHHHHHHCEE
ITQDDFQRISDITPVLGDFKPSGKYMMEDLHKIGGLPAVLKYLLKEGKLHGDCLTVTGKT
ECHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEECCHH
LAENVETALDLDFDSQDIMRPLKNPIKATGHLQILYGNLAQGGSVAKISGKEGEFFKGTA
HHHHHHHHEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCCCEEECHH
RVFDGEQHFIDGIESGRLHAGDVAVIRNIGPVGGPGMPEMLKPTSALIGAGLGKSCALIT
HEECCHHHHHCCCCCCCEECCCEEEEECCCCCCCCCCHHHHCHHHHHHHCCCCCCEEEEE
DGRFSGGTHGFVVGHIVPEAVEGGLIGLVEDDDIIEIDAVNNSISLKVSDEEIAKRRANY
CCCCCCCCCCEEEEEECHHHHCCCEEEEECCCCEEEEEECCCEEEEEECHHHHHHHHCCC
QKPTPKATRGVLAKFAKLTRPASEGCVTDL
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MEFKYNGKVESVELNKYSKTLTQDPTQPATQAMYYGIGFKDEDFKKAQVGIVSMDWDGNP
CCEEECCEEEEEECCHHHHHHCCCCCCHHHHHEEEECCCCCCCCCEEEEEEEEECCCCCC
CNMHLGTLGSKIKSSVNQTDGLIGLQFHTIGVSDGIANGKLGMRYSLVSREVIADSIETN
CEEEHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHCCC
AGAEYYDAIVAIPGCDKNMPGSIIGMARLNRPSIMVYGGTIEHGEYKGEKLNIVSAFESL
CCCCEEEEEEECCCCCCCCCCCEEEEEECCCCEEEEECCEEECCCCCCCEEHHHHHHHHH
GQKITGNISDEDYHGVICNAIPGQGACGGMYTANTLAAAIETLGMSLPYSSSNPAVSQEK
CHHEECCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
QEECDEIGLAIKNLLEKDIKPSDIMTKEAFENAITIVMVLGGSTNAVLHIIAMANAIGVE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEEEECCCCHHHEEHHHHHHHHCEE
ITQDDFQRISDITPVLGDFKPSGKYMMEDLHKIGGLPAVLKYLLKEGKLHGDCLTVTGKT
ECHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEECCHH
LAENVETALDLDFDSQDIMRPLKNPIKATGHLQILYGNLAQGGSVAKISGKEGEFFKGTA
HHHHHHHHEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCCCEEECHH
RVFDGEQHFIDGIESGRLHAGDVAVIRNIGPVGGPGMPEMLKPTSALIGAGLGKSCALIT
HEECCHHHHHCCCCCCCEECCCEEEEECCCCCCCCCCHHHHCHHHHHHHCCCCCCEEEEE
DGRFSGGTHGFVVGHIVPEAVEGGLIGLVEDDDIIEIDAVNNSISLKVSDEEIAKRRANY
CCCCCCCCCCEEEEEECHHHHCCCEEEEECCCCEEEEEECCCEEEEEECHHHHHHHHCCC
QKPTPKATRGVLAKFAKLTRPASEGCVTDL
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400210; 11337471