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The map label for this gene is ilvB

Identifier: 15673206

GI number: 15673206

Start: 1247599

End: 1249221

Strand: Direct

Name: ilvB

Synonym: L0078

Alternate gene names: 15673206

Gene position: 1247599-1249221 (Clockwise)

Preceding gene: 15673205

Following gene: 15673207

Centisome position: 52.74

GC content: 38.2

Gene sequence:

>1623_bases
ATGCTCCCCTTGTATGATGCGATTCATAATTTTGAAGGAATTCAACATATTTTAGCCCGTCATGAGCAAGGAGCAACGCA
TGAAGCCGAAGGTTACGCTAAATCGTCTGGTAAAGTTGGTGTCGTCGTTGTTACGTCTGGACCGGGAGCGACTAATGCAG
TAACCGGAATTGCTGACGCTTATCTTGATTCAGTCCCATTGTTAGTTTTCACAGGTCAAGTTGGCCCTCTGTCAATTGGT
AAAGATGCTTTTCAAGAAGCAGATACTGTTGGAATTACAGCCCCAATTACAAAATATAATTATCAAATTAGGGAAACTGC
AGATATTCCAAGAATTGTTACAGAAGCCTATTATTTGGCAAGGACAGGACGTCCTGGACCAGTAGAAATTGATTTACCAA
AAGATGTTTCCACCCTTGAAGTCACTGAAATTAATGACCCAAGCTTGAATCTTCCTCATTATCACGAAAGTGAAAAAGCG
ACTGATGAACAATTGCAAGAATTACTGACAGAACTTTCTGTCAGTAAAAAACCAGTCATTATTGCTGGCGGAGGAATTAA
TTATTCTGGCTCAGTTGATATTTTCAGAGCATTTGTCGAAAAATATCAAATTCCAGTTGTTTCTACATTGCTTGGCTTAG
GAACATTACCAATCAGCCACGAATTGCAACTAGGAATGGCAGGAATGCACGGTTCATACGCTGCAAATATGGCTTTAGTT
GAAGCTGACTATATTATTAATTTGGGATCACGTTTTGACGATAGAGTTGTATCCAATCCTGCAAAAGTTGCTAAAAATGC
TGTCGTTGCTCATATTGATATTGACGCTGCTGAACTTGGCAAAATTGTAAAAACCGATATTCCAATCCTTTCTGATTTGA
AAGCGGCTTTAAGCAGACTTTTGCAATTAAATAAGGTCAGGACTGACTTTAATGATTGGATTAAAACTGTCACTAAAAAT
AAAGAGAAAGCACCATTTACTTATGAGCCCCAAAACCATGATATCCGTCCACAGGAAACAATTAAATTAATTGGAGAATA
CACTCAAGGAGATGCAATCATTGTGACCGATGTTGGGCAACATCAAATGTGGGTGGCGCAATATTATCCTTATAAAAATG
CAAGGCAACTTATTACTTCTGGGGGAATGGGAACGATGGGCTTTGGCATTCCTGCAGCAATCGGTGCAAAGCTGGCACAG
CCAAATAAAAATGTCATTGTTTTTGTTGGCGATGGTGGCTTTCAAATGACTAATCAAGAATTAGCATTACTTAATGGCTA
CGGTATTGCAATCAAAGTTGTTCTGATTAATAATCATTCATTGGGAATGGTACGTCAATGGCAAGAATCATTCTATGAAG
AGCGACGTTCACAATCGGTTTTTGATGTTGAACCCAATTTTCAATTGTTAGCCGAAGCTTATGGCATCAAACATGTTAAG
TTAGATAATCCAAAAACTTTGGCTGATGATTTAAAAATTATTACAGAAGATGAGCCAATGCTTATTGAAGTTCTAATTTC
AAAATCTGAACATGTTTTACCAATGATACCAGCTGGGTTACACAGTGACGAAATGATTGGACTTCATTTTACTGATGAGA
ATGAGGAGGTAGATAATGCGTAG

Upstream 100 bases:

>100_bases
AAAAATATAGTTAGAAAAACCTACTTCCGGTTCCCAACTTGTTCTCCAAACCTTAAAAGAACTTGGAGTAGAAATTATTT
TTGGTTATCCTGGTGGGGCC

Downstream 100 bases:

>100_bases
AATGATTATCGCAAAACTTCATAATGTAACAGGAATTATGAATCGATTTACCGCCGTTCTCAATCGAAGGCAAGTGAACA
TTCTCTCAATTACCGCTGGA

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MLPLYDAIHNFEGIQHILARHEQGATHEAEGYAKSSGKVGVVVVTSGPGATNAVTGIADAYLDSVPLLVFTGQVGPLSIG
KDAFQEADTVGITAPITKYNYQIRETADIPRIVTEAYYLARTGRPGPVEIDLPKDVSTLEVTEINDPSLNLPHYHESEKA
TDEQLQELLTELSVSKKPVIIAGGGINYSGSVDIFRAFVEKYQIPVVSTLLGLGTLPISHELQLGMAGMHGSYAANMALV
EADYIINLGSRFDDRVVSNPAKVAKNAVVAHIDIDAAELGKIVKTDIPILSDLKAALSRLLQLNKVRTDFNDWIKTVTKN
KEKAPFTYEPQNHDIRPQETIKLIGEYTQGDAIIVTDVGQHQMWVAQYYPYKNARQLITSGGMGTMGFGIPAAIGAKLAQ
PNKNVIVFVGDGGFQMTNQELALLNGYGIAIKVVLINNHSLGMVRQWQESFYEERRSQSVFDVEPNFQLLAEAYGIKHVK
LDNPKTLADDLKIITEDEPMLIEVLISKSEHVLPMIPAGLHSDEMIGLHFTDENEEVDNA

Sequences:

>Translated_540_residues
MLPLYDAIHNFEGIQHILARHEQGATHEAEGYAKSSGKVGVVVVTSGPGATNAVTGIADAYLDSVPLLVFTGQVGPLSIG
KDAFQEADTVGITAPITKYNYQIRETADIPRIVTEAYYLARTGRPGPVEIDLPKDVSTLEVTEINDPSLNLPHYHESEKA
TDEQLQELLTELSVSKKPVIIAGGGINYSGSVDIFRAFVEKYQIPVVSTLLGLGTLPISHELQLGMAGMHGSYAANMALV
EADYIINLGSRFDDRVVSNPAKVAKNAVVAHIDIDAAELGKIVKTDIPILSDLKAALSRLLQLNKVRTDFNDWIKTVTKN
KEKAPFTYEPQNHDIRPQETIKLIGEYTQGDAIIVTDVGQHQMWVAQYYPYKNARQLITSGGMGTMGFGIPAAIGAKLAQ
PNKNVIVFVGDGGFQMTNQELALLNGYGIAIKVVLINNHSLGMVRQWQESFYEERRSQSVFDVEPNFQLLAEAYGIKHVK
LDNPKTLADDLKIITEDEPMLIEVLISKSEHVLPMIPAGLHSDEMIGLHFTDENEEVDNA
>Mature_540_residues
MLPLYDAIHNFEGIQHILARHEQGATHEAEGYAKSSGKVGVVVVTSGPGATNAVTGIADAYLDSVPLLVFTGQVGPLSIG
KDAFQEADTVGITAPITKYNYQIRETADIPRIVTEAYYLARTGRPGPVEIDLPKDVSTLEVTEINDPSLNLPHYHESEKA
TDEQLQELLTELSVSKKPVIIAGGGINYSGSVDIFRAFVEKYQIPVVSTLLGLGTLPISHELQLGMAGMHGSYAANMALV
EADYIINLGSRFDDRVVSNPAKVAKNAVVAHIDIDAAELGKIVKTDIPILSDLKAALSRLLQLNKVRTDFNDWIKTVTKN
KEKAPFTYEPQNHDIRPQETIKLIGEYTQGDAIIVTDVGQHQMWVAQYYPYKNARQLITSGGMGTMGFGIPAAIGAKLAQ
PNKNVIVFVGDGGFQMTNQELALLNGYGIAIKVVLINNHSLGMVRQWQESFYEERRSQSVFDVEPNFQLLAEAYGIKHVK
LDNPKTLADDLKIITEDEPMLIEVLISKSEHVLPMIPAGLHSDEMIGLHFTDENEEVDNA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family

Homologues:

Organism=Homo sapiens, GI93004078, Length=529, Percent_Identity=26.6540642722117, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI21361361, Length=461, Percent_Identity=25.8134490238612, Blast_Score=134, Evalue=3e-31,
Organism=Escherichia coli, GI87081685, Length=537, Percent_Identity=43.0167597765363, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI1790104, Length=529, Percent_Identity=42.7221172022684, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1786717, Length=487, Percent_Identity=33.6755646817248, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1787096, Length=518, Percent_Identity=25.6756756756757, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI1788716, Length=444, Percent_Identity=23.4234234234234, Blast_Score=108, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17531299, Length=460, Percent_Identity=28.0434782608696, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17531301, Length=460, Percent_Identity=28.0434782608696, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17542570, Length=552, Percent_Identity=24.6376811594203, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6323755, Length=561, Percent_Identity=39.7504456327986, Blast_Score=398, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6320123, Length=548, Percent_Identity=21.8978102189781, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320816, Length=458, Percent_Identity=25.1091703056769, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=467, Percent_Identity=25.0535331905782, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323073, Length=466, Percent_Identity=25.9656652360515, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6323163, Length=467, Percent_Identity=24.4111349036403, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922626, Length=549, Percent_Identity=25.5009107468124, Blast_Score=149, Evalue=6e-36,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): ILVB_LACLA (Q02137)

Other databases:

- EMBL:   U92974
- EMBL:   AE005176
- PIR:   H86777
- PIR:   S35138
- RefSeq:   NP_267380.1
- ProteinModelPortal:   Q02137
- SMR:   Q02137
- GeneID:   1114873
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0078
- NMPDR:   fig|272623.1.peg.1259
- HOGENOM:   HBG323037
- ProtClustDB:   PRK07282
- BioCyc:   LLAC272623:L0078-MONOMER
- BRENDA:   2.2.1.6
- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00118

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N

EC number: =2.2.1.6

Molecular weight: Translated: 59094; Mature: 59094

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00187 TPP_ENZYMES

Important sites: BINDING 57-57 BINDING 159-159

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPLYDAIHNFEGIQHILARHEQGATHEAEGYAKSSGKVGVVVVTSGPGATNAVTGIADA
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHH
YLDSVPLLVFTGQVGPLSIGKDAFQEADTVGITAPITKYNYQIRETADIPRIVTEAYYLA
HHCCCCEEEEECCCCCCCCCHHHHHCCCCCEEEECCEECCEEEECCCCCHHHHHHHHHHH
RTGRPGPVEIDLPKDVSTLEVTEINDPSLNLPHYHESEKATDEQLQELLTELSVSKKPVI
CCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEE
IAGGGINYSGSVDIFRAFVEKYQIPVVSTLLGLGTLPISHELQLGMAGMHGSYAANMALV
EECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEECCCCCCCCHHHCEEEE
EADYIINLGSRFDDRVVSNPAKVAKNAVVAHIDIDAAELGKIVKTDIPILSDLKAALSRL
EECEEEECCCCCCHHHHCCHHHHHCCEEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHH
LQLNKVRTDFNDWIKTVTKNKEKAPFTYEPQNHDIRPQETIKLIGEYTQGDAIIVTDVGQ
HHHHHHHCCHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHCCCCCCEEEEEECCC
HQMWVAQYYPYKNARQLITSGGMGTMGFGIPAAIGAKLAQPNKNVIVFVGDGGFQMTNQE
CCEEEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCEECCCC
LALLNGYGIAIKVVLINNHSLGMVRQWQESFYEERRSQSVFDVEPNFQLLAEAYGIKHVK
EEEEECCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHCEEEEE
LDNPKTLADDLKIITEDEPMLIEVLISKSEHVLPMIPAGLHSDEMIGLHFTDENEEVDNA
ECCCCHHHCCCEEEECCCCEEEEEEECCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MLPLYDAIHNFEGIQHILARHEQGATHEAEGYAKSSGKVGVVVVTSGPGATNAVTGIADA
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHH
YLDSVPLLVFTGQVGPLSIGKDAFQEADTVGITAPITKYNYQIRETADIPRIVTEAYYLA
HHCCCCEEEEECCCCCCCCCHHHHHCCCCCEEEECCEECCEEEECCCCCHHHHHHHHHHH
RTGRPGPVEIDLPKDVSTLEVTEINDPSLNLPHYHESEKATDEQLQELLTELSVSKKPVI
CCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEE
IAGGGINYSGSVDIFRAFVEKYQIPVVSTLLGLGTLPISHELQLGMAGMHGSYAANMALV
EECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEECCCCCCCCHHHCEEEE
EADYIINLGSRFDDRVVSNPAKVAKNAVVAHIDIDAAELGKIVKTDIPILSDLKAALSRL
EECEEEECCCCCCHHHHCCHHHHHCCEEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHH
LQLNKVRTDFNDWIKTVTKNKEKAPFTYEPQNHDIRPQETIKLIGEYTQGDAIIVTDVGQ
HHHHHHHCCHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHCCCCCCEEEEEECCC
HQMWVAQYYPYKNARQLITSGGMGTMGFGIPAAIGAKLAQPNKNVIVFVGDGGFQMTNQE
CCEEEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCEECCCC
LALLNGYGIAIKVVLINNHSLGMVRQWQESFYEERRSQSVFDVEPNFQLLAEAYGIKHVK
EEEEECCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHCEEEEE
LDNPKTLADDLKIITEDEPMLIEVLISKSEHVLPMIPAGLHSDEMIGLHFTDENEEVDNA
ECCCCHHHCCCEEEECCCCEEEEEEECCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400210; 11337471