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The map label for this gene is potB

Identifier: 15673146

GI number: 15673146

Start: 1176964

End: 1177752

Strand: Direct

Name: potB

Synonym: L176724

Alternate gene names: 15673146

Gene position: 1176964-1177752 (Clockwise)

Preceding gene: 15673145

Following gene: 15673147

Centisome position: 49.75

GC content: 36.88

Gene sequence:

>789_bases
GTGACCAAAAGAATTTATTCTGTTCCCTATTTTCTCTGGATTCTTTTCTTTGTTATCGTCCCTTTGGCCATGCTTTTATG
GCAATCATTTTTTGATTTAAATGGTCACTTTACCCTTAACAATTATATCCTCTATTTTTCTAGCGGAACTTACCTCTTAA
TGACATGGAACTCTATTCTCTACGCACTGATTATTACTGTAGCGACCCTTGTCATCTCCTACCCCGTAGCTTATGTTTTG
ACCAAACTAAAAATGAAACAATTTTGGCTCATGCTTATTATTTTACCTACTTGGGTTAACTTGTTACTCAAAGCTTATGC
CTTTATTGGAATTTTTGGTAAATATGGTTCAGTTAATAACTTTTTACAGTTTTTGGGAATTGGACGGACACAGATTCTAT
TTACTGACCTAAGTTTTATTTTGGTCGCATCATATATTTCCATTCCCTTTATGGTTTTACCAATTTTTAATGCTTTAAAT
GATTTAGATGATAATTTGGTAAATGCGAGTGCCGATTTAGGCGCCTCACGTTTCCAAACTTTTACTCAAGTGATTTGGCC
ACTTTCATTAAACGGTGTTAGAGCTGGAGTGCAGGCTGTCTTTATTCCTAGTTTGTCACTCTTCATGTTGACGCGTTTAA
TCGGTGGGAATAAAGTCATCACACTCGGTACGGCCGTTGAAGAACACTTCCTAACGACACAAAACTGGGGAATGGGTTCA
ACCATTGCTGTTGTTTTAGTTGTAGCCATGTTGCTTGTCATGAGAATGACGAAAGAAAGGAGAGATTGA

Upstream 100 bases:

>100_bases
ACACGATGGAATTGACAGAACTTGCTGAAACAGTGAATGAAATTTTACAAAAGCAAGAGAATGAACCTGAGTCAGAAAAT
AAAGAAAGTGGGGCCAATAA

Downstream 100 bases:

>100_bases
TTTGCGAAATAAAAATTCACTTGGTAAAGTTTACCTCACCTTTGTCCTCATCCTTTTATATGCACCTATTTTTTACCTTA
TTTTTTACTCATTCAACTCC

Product: spermidine/putrescine ABC transporter permease protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MTKRIYSVPYFLWILFFVIVPLAMLLWQSFFDLNGHFTLNNYILYFSSGTYLLMTWNSILYALIITVATLVISYPVAYVL
TKLKMKQFWLMLIILPTWVNLLLKAYAFIGIFGKYGSVNNFLQFLGIGRTQILFTDLSFILVASYISIPFMVLPIFNALN
DLDDNLVNASADLGASRFQTFTQVIWPLSLNGVRAGVQAVFIPSLSLFMLTRLIGGNKVITLGTAVEEHFLTTQNWGMGS
TIAVVLVVAMLLVMRMTKERRD

Sequences:

>Translated_262_residues
MTKRIYSVPYFLWILFFVIVPLAMLLWQSFFDLNGHFTLNNYILYFSSGTYLLMTWNSILYALIITVATLVISYPVAYVL
TKLKMKQFWLMLIILPTWVNLLLKAYAFIGIFGKYGSVNNFLQFLGIGRTQILFTDLSFILVASYISIPFMVLPIFNALN
DLDDNLVNASADLGASRFQTFTQVIWPLSLNGVRAGVQAVFIPSLSLFMLTRLIGGNKVITLGTAVEEHFLTTQNWGMGS
TIAVVLVVAMLLVMRMTKERRD
>Mature_261_residues
TKRIYSVPYFLWILFFVIVPLAMLLWQSFFDLNGHFTLNNYILYFSSGTYLLMTWNSILYALIITVATLVISYPVAYVLT
KLKMKQFWLMLIILPTWVNLLLKAYAFIGIFGKYGSVNNFLQFLGIGRTQILFTDLSFILVASYISIPFMVLPIFNALND
LDDNLVNASADLGASRFQTFTQVIWPLSLNGVRAGVQAVFIPSLSLFMLTRLIGGNKVITLGTAVEEHFLTTQNWGMGST
IAVVLVVAMLLVMRMTKERRD

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=248, Percent_Identity=35.0806451612903, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1787080, Length=237, Percent_Identity=34.5991561181435, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1787713, Length=258, Percent_Identity=29.4573643410853, Blast_Score=100, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29735; Mature: 29604

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRIYSVPYFLWILFFVIVPLAMLLWQSFFDLNGHFTLNNYILYFSSGTYLLMTWNSIL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCEEEEEHHHHH
YALIITVATLVISYPVAYVLTKLKMKQFWLMLIILPTWVNLLLKAYAFIGIFGKYGSVNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FLQFLGIGRTQILFTDLSFILVASYISIPFMVLPIFNALNDLDDNLVNASADLGASRFQT
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHH
FTQVIWPLSLNGVRAGVQAVFIPSLSLFMLTRLIGGNKVITLGTAVEEHFLTTQNWGMGS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCCCCCCCH
TIAVVLVVAMLLVMRMTKERRD
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKRIYSVPYFLWILFFVIVPLAMLLWQSFFDLNGHFTLNNYILYFSSGTYLLMTWNSIL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCEEEEEHHHHH
YALIITVATLVISYPVAYVLTKLKMKQFWLMLIILPTWVNLLLKAYAFIGIFGKYGSVNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
FLQFLGIGRTQILFTDLSFILVASYISIPFMVLPIFNALNDLDDNLVNASADLGASRFQT
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHH
FTQVIWPLSLNGVRAGVQAVFIPSLSLFMLTRLIGGNKVITLGTAVEEHFLTTQNWGMGS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCCCCCCCH
TIAVVLVVAMLLVMRMTKERRD
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]