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The map label for this gene is thrB

Identifier: 15673142

GI number: 15673142

Start: 1172344

End: 1173234

Strand: Direct

Name: thrB

Synonym: L0091

Alternate gene names: 15673142

Gene position: 1172344-1173234 (Clockwise)

Preceding gene: 15673141

Following gene: 15673143

Centisome position: 49.56

GC content: 35.35

Gene sequence:

>891_bases
ATGAAAATTATTGTACCAGCGACATCAGCAAATTTAGGCGCAGGTTTTGATTCAATTGGTATTGCGGTCAACTTGTATTT
GACAGTGGAAGTACTTGAAGAAAGTAATGATTGGAAAATTGACCATGATTTAGGAAATAATATTCCTACTGACGAAAAAA
ATTTATTACTGACCACGCTGTCAGCAGTTTTGAAAGCTAAAAATAGTAGTCTGTCAGCAAAATATCATTTGAAAATGACT
TCGGAAGTTCCTTTAGCAAGAGGTTTGGGTTCATCAAGTTCAGTCATTATTGCTGGGATTGAATTAGCTAATCAACTAGC
AAAGCTAAATTTGACAACAGAAGAAAAACTTGAACTTGCTTGTGAAATTGAAGGGCACCCTGATAATGTTGCGCCAGCAC
TTTTAGGAAATTTAGTTATAGCAAGTACTGTTGCTGACAAAACGAATTATGTTAGCTCAGACTTTCCTTCATGTAAACTT
CTAGCTTTTGTTCCAGACTATGAGTTAAAAACTGTTGAAAGTCGAAAAGTTTTACCTAAGGAATTGGCTTACAAAGAGGC
CGTTGCTGCCAGCTCTATTGCAAATGTACTGACAGCCAGTCTTTTGACAAAAAATTTAAAAGTTGCTGGGCAAATGATAG
AATCTGACCATTTTCATGAAAATTATCGCGCTTCTTTAGTTCCTGAACTGAAAATTTTGCGAGAAATTGGCCATGAATTT
GGTGCTTATGGAACTTATTTATCTGGGGCGGGACCAACAGTAATGCTACTTTTACCCGATGATAAGCTGAATTTACTGAC
AGAAAAAATTAATGAGCAAAATTTATCTGGTCAACTTTATTCCTTGGAAGTTGACAGTAATGGATTACAAGTAAAAGAAT
CTGTATTATAA

Upstream 100 bases:

>100_bases
CAGATTAACCGCGTTCAACTTTTGGCCATTCAAGATAAATTAAAAGAAGAAGTAGATTTTAAATTACTTAATTATTTTAA
AGTTTTGGGGGACTAGGAAA

Downstream 100 bases:

>100_bases
ATACAGATTTTTTTCTTGATTATTTTATATATAAAATGATATAATCATTAAAGCAAAAAAGAATGTAAAGTAGTTCACTA
ACTTTCATTTTATTTGTCAG

Product: homoserine kinase

Products: NA

Alternate protein names: HK; HSK

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKIIVPATSANLGAGFDSIGIAVNLYLTVEVLEESNDWKIDHDLGNNIPTDEKNLLLTTLSAVLKAKNSSLSAKYHLKMT
SEVPLARGLGSSSSVIIAGIELANQLAKLNLTTEEKLELACEIEGHPDNVAPALLGNLVIASTVADKTNYVSSDFPSCKL
LAFVPDYELKTVESRKVLPKELAYKEAVAASSIANVLTASLLTKNLKVAGQMIESDHFHENYRASLVPELKILREIGHEF
GAYGTYLSGAGPTVMLLLPDDKLNLLTEKINEQNLSGQLYSLEVDSNGLQVKESVL

Sequences:

>Translated_296_residues
MKIIVPATSANLGAGFDSIGIAVNLYLTVEVLEESNDWKIDHDLGNNIPTDEKNLLLTTLSAVLKAKNSSLSAKYHLKMT
SEVPLARGLGSSSSVIIAGIELANQLAKLNLTTEEKLELACEIEGHPDNVAPALLGNLVIASTVADKTNYVSSDFPSCKL
LAFVPDYELKTVESRKVLPKELAYKEAVAASSIANVLTASLLTKNLKVAGQMIESDHFHENYRASLVPELKILREIGHEF
GAYGTYLSGAGPTVMLLLPDDKLNLLTEKINEQNLSGQLYSLEVDSNGLQVKESVL
>Mature_296_residues
MKIIVPATSANLGAGFDSIGIAVNLYLTVEVLEESNDWKIDHDLGNNIPTDEKNLLLTTLSAVLKAKNSSLSAKYHLKMT
SEVPLARGLGSSSSVIIAGIELANQLAKLNLTTEEKLELACEIEGHPDNVAPALLGNLVIASTVADKTNYVSSDFPSCKL
LAFVPDYELKTVESRKVLPKELAYKEAVAASSIANVLTASLLTKNLKVAGQMIESDHFHENYRASLVPELKILREIGHEF
GAYGTYLSGAGPTVMLLLPDDKLNLLTEKINEQNLSGQLYSLEVDSNGLQVKESVL

Specific function: Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate

COG id: COG0083

COG function: function code E; Homoserine kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. Homoserine kinase subfamily

Homologues:

Organism=Escherichia coli, GI1786184, Length=317, Percent_Identity=24.9211356466877, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321814, Length=329, Percent_Identity=28.8753799392097, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KHSE_LACLA (Q9CGD7)

Other databases:

- EMBL:   AE005176
- PIR:   H86769
- RefSeq:   NP_267316.1
- ProteinModelPortal:   Q9CGD7
- GeneID:   1114802
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0091
- NMPDR:   fig|272623.1.peg.1194
- HOGENOM:   HBG646290
- OMA:   NNALMST
- ProtClustDB:   PRK01212
- BioCyc:   LLAC272623:L0091-MONOMER
- BRENDA:   2.7.1.39
- GO:   GO:0005737
- HAMAP:   MF_00384
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR006203
- InterPro:   IPR000870
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF000676
- PRINTS:   PR00958
- TIGRFAMs:   TIGR00191

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.39

Molecular weight: Translated: 31919; Mature: 31919

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00627 GHMP_KINASES_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIVPATSANLGAGFDSIGIAVNLYLTVEVLEESNDWKIDHDLGNNIPTDEKNLLLTTL
CEEEEECCCCCCCCCCCCCCEEEEEEEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHH
SAVLKAKNSSLSAKYHLKMTSEVPLARGLGSSSSVIIAGIELANQLAKLNLTTEEKLELA
HHHHHHCCCCCCEEEEEEECCCCCHHHCCCCCCCEEEEEHHHHHHHHHCCCCCHHCEEEE
CEIEGHPDNVAPALLGNLVIASTVADKTNYVSSDFPSCKLLAFVPDYELKTVESRKVLPK
EEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEECCHHCCCHH
ELAYKEAVAASSIANVLTASLLTKNLKVAGQMIESDHFHENYRASLVPELKILREIGHEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHH
GAYGTYLSGAGPTVMLLLPDDKLNLLTEKINEQNLSGQLYSLEVDSNGLQVKESVL
CCCCCEECCCCCEEEEEECCCHHHHHHHHHCCCCCCCEEEEEEECCCCCEEHHCCC
>Mature Secondary Structure
MKIIVPATSANLGAGFDSIGIAVNLYLTVEVLEESNDWKIDHDLGNNIPTDEKNLLLTTL
CEEEEECCCCCCCCCCCCCCEEEEEEEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHH
SAVLKAKNSSLSAKYHLKMTSEVPLARGLGSSSSVIIAGIELANQLAKLNLTTEEKLELA
HHHHHHCCCCCCEEEEEEECCCCCHHHCCCCCCCEEEEEHHHHHHHHHCCCCCHHCEEEE
CEIEGHPDNVAPALLGNLVIASTVADKTNYVSSDFPSCKLLAFVPDYELKTVESRKVLPK
EEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEECCHHCCCHH
ELAYKEAVAASSIANVLTASLLTKNLKVAGQMIESDHFHENYRASLVPELKILREIGHEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHH
GAYGTYLSGAGPTVMLLLPDDKLNLLTEKINEQNLSGQLYSLEVDSNGLQVKESVL
CCCCCEECCCCCEEEEEECCCHHHHHHHHHCCCCCCCEEEEEEECCCCCEEHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471