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The map label for this gene is pacA

Identifier: 15673117

GI number: 15673117

Start: 1149436

End: 1150407

Strand: Direct

Name: pacA

Synonym: L149164

Alternate gene names: NA

Gene position: 1149436-1150407 (Clockwise)

Preceding gene: 15673116

Following gene: 15673118

Centisome position: 48.59

GC content: 36.73

Gene sequence:

>972_bases
ATGTGTACCAGTTTTCAACTAAAGTCAAGTGACGGTGGATTGGTTTTTGCTAGAACAATGGATTGGCATACTTTTAATGC
CGCTGCCCTTGTTCTACCAAGAAATTATGAATGGACAAGCGTCTATAACAATCAAAAAATGATTAATCCATATGCCATCC
TTGGCGTCGGAAATAGCGAAATGAGATTACATGCTGATATTTCAGATGGTGTAAATGAATATGGCTTGGCGGCACAAAAA
CTAACTTTTTCAAATCAGTCAGATTATGCTAAAGAAGCAGAGAAGGGAAAAGTTCAACTTGCTGCTTTTGAATTTTTGCT
TTGGTTGCTGGGGAATTGCCGTTCAATTGAGGAAGTTCGTCAGAATATTGATAATGTACAGTTGATGACTGATGAAAATG
CAATCTACAAATTTGGACGTAATGATTTACATTTTTCTGCGACAGATCCAAGCGGGCAAATGATTAATATTGAACCGCAT
GGTGGACGATTGGTCATTTCAGATAATCCAATTGGTGTGGTTACAAATGCTCCTAAATTTGAGAATGAAGTCGAGAAATT
AAGGACTTATATGGATATCAATTTACTGACAGAAAGTGAAAATACTTCTGTCAGTAAAAATGAGGATAGTAATTCATCAT
TAGAAAATCTGTCAGTAAAAAATGATAATCCTAACCAAATTTCGACAGGAAGTTTCAATGGGAAACCAGTTTTTCCTGGA
GGATTCACACCAACAGCTCGATTTATTCGAGCAGCTATTTACAAAGAAAGAGCGGTGTATCCTAAAGATGAACAGCAAAA
TATTGTTGAGGCATGGCATATTTTAAATGGTGTTACAGTTCCAAAGTCTGAAGGCCGCTCAGGGACTTATACTGTTTATC
GCAGTGCTGTGGAAGTGAACAGTCGCAGACTTTACTTACAAACGTATGATGATTTAAGTATACAAGTCTATCGTTTCCCA
GATGAATTATAA

Upstream 100 bases:

>100_bases
AGTTTTTGTTATAGGATTGAAGTTTATAGTTCTGCTAGTATCTTTATCAGTAAAACTTTTGATAAAATAAAGAGATGACA
AATTAATTAAGGGGAGAAAA

Downstream 100 bases:

>100_bases
TCTTAAGATTTTGTTATAATGAAAACAAAATTATTTTAGGAGAATTTAATGCATCTTATCGTTATTTTAGGCGCACTTAT
TTCAATATCTTTTACAACTA

Product: penicillin acylase

Products: NA

Alternate protein names: Penicillin V amidase; PVA [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MCTSFQLKSSDGGLVFARTMDWHTFNAAALVLPRNYEWTSVYNNQKMINPYAILGVGNSEMRLHADISDGVNEYGLAAQK
LTFSNQSDYAKEAEKGKVQLAAFEFLLWLLGNCRSIEEVRQNIDNVQLMTDENAIYKFGRNDLHFSATDPSGQMINIEPH
GGRLVISDNPIGVVTNAPKFENEVEKLRTYMDINLLTESENTSVSKNEDSNSSLENLSVKNDNPNQISTGSFNGKPVFPG
GFTPTARFIRAAIYKERAVYPKDEQQNIVEAWHILNGVTVPKSEGRSGTYTVYRSAVEVNSRRLYLQTYDDLSIQVYRFP
DEL

Sequences:

>Translated_323_residues
MCTSFQLKSSDGGLVFARTMDWHTFNAAALVLPRNYEWTSVYNNQKMINPYAILGVGNSEMRLHADISDGVNEYGLAAQK
LTFSNQSDYAKEAEKGKVQLAAFEFLLWLLGNCRSIEEVRQNIDNVQLMTDENAIYKFGRNDLHFSATDPSGQMINIEPH
GGRLVISDNPIGVVTNAPKFENEVEKLRTYMDINLLTESENTSVSKNEDSNSSLENLSVKNDNPNQISTGSFNGKPVFPG
GFTPTARFIRAAIYKERAVYPKDEQQNIVEAWHILNGVTVPKSEGRSGTYTVYRSAVEVNSRRLYLQTYDDLSIQVYRFP
DEL
>Mature_323_residues
MCTSFQLKSSDGGLVFARTMDWHTFNAAALVLPRNYEWTSVYNNQKMINPYAILGVGNSEMRLHADISDGVNEYGLAAQK
LTFSNQSDYAKEAEKGKVQLAAFEFLLWLLGNCRSIEEVRQNIDNVQLMTDENAIYKFGRNDLHFSATDPSGQMINIEPH
GGRLVISDNPIGVVTNAPKFENEVEKLRTYMDINLLTESENTSVSKNEDSNSSLENLSVKNDNPNQISTGSFNGKPVFPG
GFTPTARFIRAAIYKERAVYPKDEQQNIVEAWHILNGVTVPKSEGRSGTYTVYRSAVEVNSRRLYLQTYDDLSIQVYRFP
DEL

Specific function: The enzyme catalyzes the conversion of penicillin to 6- aminopenicillanate The precursor, furthermore, acts as a self- processing peptidase that cleaves off the propeptide. All peptidase activity is lost on conversion to the mature peptidase [H]

COG id: COG3049

COG function: function code M; Penicillin V acylase and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C59 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003199 [H]

Pfam domain/function: PF02275 CBAH [H]

EC number: =3.5.1.11 [H]

Molecular weight: Translated: 36367; Mature: 36367

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTSFQLKSSDGGLVFARTMDWHTFNAAALVLPRNYEWTSVYNNQKMINPYAILGVGNSE
CCCCEEEECCCCCEEEEEEECCEEECEEEEEEECCCEEEEEECCCEEECCEEEEEECCCC
MRLHADISDGVNEYGLAAQKLTFSNQSDYAKEAEKGKVQLAAFEFLLWLLGNCRSIEEVR
EEEEECHHCCCHHHCCEEEEEEECCCCHHHHHHHCCCEEHHHHHHHHHHHCCCCCHHHHH
QNIDNVQLMTDENAIYKFGRNDLHFSATDPSGQMINIEPHGGRLVISDNPIGVVTNAPKF
HCCCCEEEEECCCCEEECCCCCEEEEECCCCCCEEEECCCCCEEEECCCCEEEEECCCCH
ENEVEKLRTYMDINLLTESENTSVSKNEDSNSSLENLSVKNDNPNQISTGSFNGKPVFPG
HHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCHHEEEECCCCCCEEECCCCCCCCCCCC
GFTPTARFIRAAIYKERAVYPKDEQQNIVEAWHILNGVTVPKSEGRSGTYTVYRSAVEVN
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEC
SRRLYLQTYDDLSIQVYRFPDEL
CCEEEEEECCCCEEEEEECCCCC
>Mature Secondary Structure
MCTSFQLKSSDGGLVFARTMDWHTFNAAALVLPRNYEWTSVYNNQKMINPYAILGVGNSE
CCCCEEEECCCCCEEEEEEECCEEECEEEEEEECCCEEEEEECCCEEECCEEEEEECCCC
MRLHADISDGVNEYGLAAQKLTFSNQSDYAKEAEKGKVQLAAFEFLLWLLGNCRSIEEVR
EEEEECHHCCCHHHCCEEEEEEECCCCHHHHHHHCCCEEHHHHHHHHHHHCCCCCHHHHH
QNIDNVQLMTDENAIYKFGRNDLHFSATDPSGQMINIEPHGGRLVISDNPIGVVTNAPKF
HCCCCEEEEECCCCEEECCCCCEEEEECCCCCCEEEECCCCCEEEECCCCEEEEECCCCH
ENEVEKLRTYMDINLLTESENTSVSKNEDSNSSLENLSVKNDNPNQISTGSFNGKPVFPG
HHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCHHEEEECCCCCCEEECCCCCCCCCCCC
GFTPTARFIRAAIYKERAVYPKDEQQNIVEAWHILNGVTVPKSEGRSGTYTVYRSAVEVN
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEC
SRRLYLQTYDDLSIQVYRFPDEL
CCEEEEEECCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3026906 [H]