Click here to switch to the map view.

The map label for this gene is tpiA

Identifier: 15673116

GI number: 15673116

Start: 1148515

End: 1149273

Strand: Direct

Name: tpiA

Synonym: L0006

Alternate gene names: 15673116

Gene position: 1148515-1149273 (Clockwise)

Preceding gene: 15673115

Following gene: 15673117

Centisome position: 48.55

GC content: 41.11

Gene sequence:

>759_bases
ATGTCACGTAAACCAATTATCGCTGGTAACTGGAAAATGAACAAAACTTTGTCAGAAGCGCAAGCTTTTGTTGAAGCTGT
AAAAAATAACTTGCCTTCATCTGATAATGTTGAATCAGTTATCGGTGCACCTGCACTTTTCCTCGCTCCTATGGCTTACC
TTCGTCAAGGTTCAGAATTGAAACTTGCTGCTGAAAATAGCTACTTTGAAAATGCTGGAGCGTTCACTGGTGAAAATAGT
CCAGCTGCTATCGTTGATTTAGGTGTTGAATACATCATCATCGGTCACAGCGAACGTCGTGAATATTTCCACGAAACTGA
CGAAGACATCAACAAAAAAGCAAAAGCAATCTTCGCTGCTGGAGCAACTCCAATCCTTTGTTGTGGTGAAACTTTGGAAA
CTTTTGAAGCTGGAAAAACAGCTGAATGGGTTTCAGGTCAAATCGAAGCTGGTCTTGCTGGCTTGAGTGCTGAACAAGTT
TCAAACTTGGTTATTGCTTATGAACCAATCTGGGCAATCGGAACTGGTAAAACTGCAACAAACGAAATCGCTGATGAAAC
TTGTGGTGTTGTTCGTTCAACAGTTGAAAAACTTTACGGAAAAGAAGTTTCAGAAGCTGTACGTATCCAATACGGTGGTT
CAGTTAAACCTGAAACAATCGAAGGATTGATGGCTAAAGAAAACATCGACGGTGCCCTCGTTGGTGGAGCTTCACTTGAA
GCTGATAGCTTCCTTGCATTGCTTGAAATGTACAAATAA

Upstream 100 bases:

>100_bases
TTTCTGCAATTTTTTTCACAATATGATAAAATAGACCTTGTAAAAGATGGACTGAAGTCCAAAATTTAAGAGGCTTAAAC
GCCCTATAAGGAGAATTAGA

Downstream 100 bases:

>100_bases
TTTTATAATTATGGGTTGAATGAAAGTACTGATTCTGTCAGTACTTTTTTCGTACATTTTTTAGTTTTTGTTATAGGATT
GAAGTTTATAGTTCTGCTAG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSRKPIIAGNWKMNKTLSEAQAFVEAVKNNLPSSDNVESVIGAPALFLAPMAYLRQGSELKLAAENSYFENAGAFTGENS
PAAIVDLGVEYIIIGHSERREYFHETDEDINKKAKAIFAAGATPILCCGETLETFEAGKTAEWVSGQIEAGLAGLSAEQV
SNLVIAYEPIWAIGTGKTATNEIADETCGVVRSTVEKLYGKEVSEAVRIQYGGSVKPETIEGLMAKENIDGALVGGASLE
ADSFLALLEMYK

Sequences:

>Translated_252_residues
MSRKPIIAGNWKMNKTLSEAQAFVEAVKNNLPSSDNVESVIGAPALFLAPMAYLRQGSELKLAAENSYFENAGAFTGENS
PAAIVDLGVEYIIIGHSERREYFHETDEDINKKAKAIFAAGATPILCCGETLETFEAGKTAEWVSGQIEAGLAGLSAEQV
SNLVIAYEPIWAIGTGKTATNEIADETCGVVRSTVEKLYGKEVSEAVRIQYGGSVKPETIEGLMAKENIDGALVGGASLE
ADSFLALLEMYK
>Mature_251_residues
SRKPIIAGNWKMNKTLSEAQAFVEAVKNNLPSSDNVESVIGAPALFLAPMAYLRQGSELKLAAENSYFENAGAFTGENSP
AAIVDLGVEYIIIGHSERREYFHETDEDINKKAKAIFAAGATPILCCGETLETFEAGKTAEWVSGQIEAGLAGLSAEQVS
NLVIAYEPIWAIGTGKTATNEIADETCGVVRSTVEKLYGKEVSEAVRIQYGGSVKPETIEGLMAKENIDGALVGGASLEA
DSFLALLEMYK

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=244, Percent_Identity=39.344262295082, Blast_Score=150, Evalue=8e-37,
Organism=Homo sapiens, GI226529917, Length=244, Percent_Identity=39.344262295082, Blast_Score=150, Evalue=9e-37,
Organism=Escherichia coli, GI1790353, Length=247, Percent_Identity=42.5101214574899, Blast_Score=187, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI17536593, Length=243, Percent_Identity=43.6213991769547, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6320255, Length=240, Percent_Identity=40.8333333333333, Blast_Score=171, Evalue=1e-43,
Organism=Drosophila melanogaster, GI28572008, Length=245, Percent_Identity=46.1224489795918, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI28572006, Length=245, Percent_Identity=46.1224489795918, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI28572004, Length=247, Percent_Identity=45.748987854251, Blast_Score=181, Evalue=4e-46,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_LACLA (P50918)

Other databases:

- EMBL:   U07640
- EMBL:   AE005176
- PIR:   F86766
- RefSeq:   NP_267290.1
- ProteinModelPortal:   P50918
- SMR:   P50918
- GeneID:   1114774
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0006
- NMPDR:   fig|272623.1.peg.1166
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   LLAC272623:L0006-MONOMER
- BioCyc:   MetaCyc:MONOMER-13055
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26906; Mature: 26774

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 96-96 ACT_SITE 168-168 BINDING 10-10 BINDING 12-12

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKPIIAGNWKMNKTLSEAQAFVEAVKNNLPSSDNVESVIGAPALFLAPMAYLRQGSEL
CCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCE
KLAAENSYFENAGAFTGENSPAAIVDLGVEYIIIGHSERREYFHETDEDINKKAKAIFAA
EEEECCCCHHCCCCCCCCCCCEEEEECCEEEEEECCHHHHHHHHCCHHHHHHHHHHHHCC
GATPILCCGETLETFEAGKTAEWVSGQIEAGLAGLSAEQVSNLVIAYEPIWAIGTGKTAT
CCCCEEECCCHHHHHHCCCCHHHHCCHHHHCCCCCCHHHHCCEEEEECCEEEEECCCCCH
NEIADETCGVVRSTVEKLYGKEVSEAVRIQYGGSVKPETIEGLMAKENIDGALVGGASLE
HHHHHHHHHHHHHHHHHHHCHHHHHHEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCC
ADSFLALLEMYK
HHHHHHHHHHCC
>Mature Secondary Structure 
SRKPIIAGNWKMNKTLSEAQAFVEAVKNNLPSSDNVESVIGAPALFLAPMAYLRQGSEL
CCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCE
KLAAENSYFENAGAFTGENSPAAIVDLGVEYIIIGHSERREYFHETDEDINKKAKAIFAA
EEEECCCCHHCCCCCCCCCCCEEEEECCEEEEEECCHHHHHHHHCCHHHHHHHHHHHHCC
GATPILCCGETLETFEAGKTAEWVSGQIEAGLAGLSAEQVSNLVIAYEPIWAIGTGKTAT
CCCCEEECCCHHHHHHCCCCHHHHCCHHHHCCCCCCHHHHCCEEEEECCEEEEECCCCCH
NEIADETCGVVRSTVEKLYGKEVSEAVRIQYGGSVKPETIEGLMAKENIDGALVGGASLE
HHHHHHHHHHHHHHHHHHHCHHHHHHEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCC
ADSFLALLEMYK
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7534588; 11337471