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The map label for this gene is nth

Identifier: 15673053

GI number: 15673053

Start: 1083852

End: 1084508

Strand: Direct

Name: nth

Synonym: L0253

Alternate gene names: 15673053

Gene position: 1083852-1084508 (Clockwise)

Preceding gene: 15673052

Following gene: 15673054

Centisome position: 45.82

GC content: 38.36

Gene sequence:

>657_bases
ATGTTAAGTAAAAAAAGATATCTTGAAGCTTTAGAAATTATTGAAGACATGTTCCCGCAAGCTCACGGAGAATTAGAGTG
GGAAACTCCTTTTCAACTATTGATTGCGACAATTTTATCTGCCCAAGCAACTGACAAAGGAGTTAATAAGGCGACGCCGG
CTCTTTTTGCCACTTTTCCAGATGCACAAACAATGTCACAGGCTAAAGTGGAAGAAATTGAAAAGCTGATTCGGACAATT
GGTCTTTACAAGACTAAAGCTAAAAATATTTTACGGACATCACAGATGTTAGTGACCGACTTTGGGGACCTTTTGCCTGA
TTTGCCAAAGGATAAAAAGGTTTTACAAACTTTACCGGGGGTGGGTCGAAAAACAGCAAATGTTGTTTTAGCAGAAGCCT
ACGGAATTCCGGGGATTGCGGTTGATACACATGTTGAACGAGTTTCTAAACGTTTGGATATCGTTCCCCAAAAAGCAACA
GTTTTAGAAGTTGAAGAAAAGTTGATGAAGCTGATTCCGCAAGAAAAATGGGTACAAGCACATCACCATCTCATTTTCTT
TGGGCGTTATCATTGTACAGCCAAAAAGCCGAAATGTGCAGACTGTCCTGTTTTAGATTATTGTAAATTTGGAAAGAAAT
ATTTAAAAAATGAATGA

Upstream 100 bases:

>100_bases
AATGGAACGCGAAGAAGCTAAGAGAAAGAAACCACAAGAAGATTTTTATATTCCTTTGGATGGACCATGGAATAGTTAAT
AGAGATAATGGACGTGCACG

Downstream 100 bases:

>100_bases
AATTAAACTTTCAAAACGTTTGAAAGCAGTAGCTAATTATGTTGATAATGGAGCAAGACTTGCTGATATTGGCTCAGACC
ATGCTTATTTACCAACTTAT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MLSKKRYLEALEIIEDMFPQAHGELEWETPFQLLIATILSAQATDKGVNKATPALFATFPDAQTMSQAKVEEIEKLIRTI
GLYKTKAKNILRTSQMLVTDFGDLLPDLPKDKKVLQTLPGVGRKTANVVLAEAYGIPGIAVDTHVERVSKRLDIVPQKAT
VLEVEEKLMKLIPQEKWVQAHHHLIFFGRYHCTAKKPKCADCPVLDYCKFGKKYLKNE

Sequences:

>Translated_218_residues
MLSKKRYLEALEIIEDMFPQAHGELEWETPFQLLIATILSAQATDKGVNKATPALFATFPDAQTMSQAKVEEIEKLIRTI
GLYKTKAKNILRTSQMLVTDFGDLLPDLPKDKKVLQTLPGVGRKTANVVLAEAYGIPGIAVDTHVERVSKRLDIVPQKAT
VLEVEEKLMKLIPQEKWVQAHHHLIFFGRYHCTAKKPKCADCPVLDYCKFGKKYLKNE
>Mature_218_residues
MLSKKRYLEALEIIEDMFPQAHGELEWETPFQLLIATILSAQATDKGVNKATPALFATFPDAQTMSQAKVEEIEKLIRTI
GLYKTKAKNILRTSQMLVTDFGDLLPDLPKDKKVLQTLPGVGRKTANVVLAEAYGIPGIAVDTHVERVSKRLDIVPQKAT
VLEVEEKLMKLIPQEKWVQAHHHLIFFGRYHCTAKKPKCADCPVLDYCKFGKKYLKNE

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=175, Percent_Identity=34.2857142857143, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI1787920, Length=212, Percent_Identity=45.7547169811321, Blast_Score=187, Evalue=3e-49,
Organism=Escherichia coli, GI1789331, Length=194, Percent_Identity=28.8659793814433, Blast_Score=74, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17554540, Length=181, Percent_Identity=35.3591160220994, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324530, Length=191, Percent_Identity=33.5078534031414, Blast_Score=89, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319304, Length=171, Percent_Identity=30.4093567251462, Blast_Score=65, Evalue=9e-12,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=36.1111111111111, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24631; Mature: 24631

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKKRYLEALEIIEDMFPQAHGELEWETPFQLLIATILSAQATDKGVNKATPALFATFP
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHEEECC
DAQTMSQAKVEEIEKLIRTIGLYKTKAKNILRTSQMLVTDFGDLLPDLPKDKKVLQTLPG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
VGRKTANVVLAEAYGIPGIAVDTHVERVSKRLDIVPQKATVLEVEEKLMKLIPQEKWVQA
CCCHHHHHHEEHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHH
HHHLIFFGRYHCTAKKPKCADCPVLDYCKFGKKYLKNE
HHHEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLSKKRYLEALEIIEDMFPQAHGELEWETPFQLLIATILSAQATDKGVNKATPALFATFP
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHEEECC
DAQTMSQAKVEEIEKLIRTIGLYKTKAKNILRTSQMLVTDFGDLLPDLPKDKKVLQTLPG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
VGRKTANVVLAEAYGIPGIAVDTHVERVSKRLDIVPQKATVLEVEEKLMKLIPQEKWVQA
CCCHHHHHHEEHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHH
HHHLIFFGRYHCTAKKPKCADCPVLDYCKFGKKYLKNE
HHHEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]