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The map label for this gene is ribB

Identifier: 15672976

GI number: 15672976

Start: 1023116

End: 1023766

Strand: Direct

Name: ribB

Synonym: L0164

Alternate gene names: 15672976

Gene position: 1023116-1023766 (Clockwise)

Preceding gene: 15672975

Following gene: 15672977

Centisome position: 43.25

GC content: 35.02

Gene sequence:

>651_bases
ATGTTTACAGGAATAATTGAAGAATTAGGGGAAGTGAGTCGCCTGCAAAAAAGAGAAAAATCCTTCAAAATTAGTATCCG
TGCTTCCTTGATTTTAGAGGATATGAAACTTGGAGATTCTATTTCAACAAATGGTTTATGCCTGACCGTTTCTGAATTTA
ATCAAAAAGAATTTACGGCTGATATTATGCCTGAAAGTCTAAATCGCTCAAATCTTGGGACCTTAAATGTGGGTGCCAAA
GTTAACTTAGAACGAGCGATGCCATTAAATGGCCGATTTGGTGGGCATATTGTGTCAGGGCATATTGATGGAGTTGGCCA
GATTTTAGAAGTAAAAAAAGAAGAAAATGCCATCTGGTTTGAAATCAAAACGAGCTTAAATTTAATGAGATACACCGTCG
AAAAAGGCTCGATTACGATTGATGGGATTAGTCTTACAGTTGCTCAGGTTAAGTCGAAATCTTTTAGTGTTTCCATTATT
CCACATACGCTCAAAGAAACGATAATGGCAGATAAAAAAGTGGGTGATACTGTTAATTTAGAAAATGATATTTTGGGGAA
ATATATAGAAAAATTATGGGGCGATAGTAGTTCTTCGATGAAAGAATCTTCAGTAACTAAAGAAAAGCTATTTAACAACG
GATTTTTATAG

Upstream 100 bases:

>100_bases
AAGGACATGGAATCGAGCAACCCAATCAAGCCTATCTCCTAGAAGGGGCTCAAATAACCTATCTAGGAGAAGATATTTTA
TTAGAAAGTGAGCTTCTTTA

Downstream 100 bases:

>100_bases
GAGATAAAATGTTCCAATATAATAAAGTAGAAGAAGCACTTGCTGCACTTAAAGCAGGGGAAATGATTATCGTTAGTGAC
GATGAAAATAGAGAAAATGA

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MFTGIIEELGEVSRLQKREKSFKISIRASLILEDMKLGDSISTNGLCLTVSEFNQKEFTADIMPESLNRSNLGTLNVGAK
VNLERAMPLNGRFGGHIVSGHIDGVGQILEVKKEENAIWFEIKTSLNLMRYTVEKGSITIDGISLTVAQVKSKSFSVSII
PHTLKETIMADKKVGDTVNLENDILGKYIEKLWGDSSSSMKESSVTKEKLFNNGFL

Sequences:

>Translated_216_residues
MFTGIIEELGEVSRLQKREKSFKISIRASLILEDMKLGDSISTNGLCLTVSEFNQKEFTADIMPESLNRSNLGTLNVGAK
VNLERAMPLNGRFGGHIVSGHIDGVGQILEVKKEENAIWFEIKTSLNLMRYTVEKGSITIDGISLTVAQVKSKSFSVSII
PHTLKETIMADKKVGDTVNLENDILGKYIEKLWGDSSSSMKESSVTKEKLFNNGFL
>Mature_216_residues
MFTGIIEELGEVSRLQKREKSFKISIRASLILEDMKLGDSISTNGLCLTVSEFNQKEFTADIMPESLNRSNLGTLNVGAK
VNLERAMPLNGRFGGHIVSGHIDGVGQILEVKKEENAIWFEIKTSLNLMRYTVEKGSITIDGISLTVAQVKSKSFSVSII
PHTLKETIMADKKVGDTVNLENDILGKYIEKLWGDSSSSMKESSVTKEKLFNNGFL

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=187, Percent_Identity=39.572192513369, Blast_Score=133, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6319733, Length=203, Percent_Identity=44.3349753694581, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 23890; Mature: 23890

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIIEELGEVSRLQKREKSFKISIRASLILEDMKLGDSISTNGLCLTVSEFNQKEFTA
CCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEEHHHHCCCCCCCCCEEEEEECCCCHHHHH
DIMPESLNRSNLGTLNVGAKVNLERAMPLNGRFGGHIVSGHIDGVGQILEVKKEENAIWF
HCCCCCCCCCCCCEEECCCEEEHHHCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCEEEE
EIKTSLNLMRYTVEKGSITIDGISLTVAQVKSKSFSVSIIPHTLKETIMADKKVGDTVNL
EEECCCEEEEEEEECCCEEEECEEEEEEECCCCCEEEEECCHHHHHHHHHCCCCCCCCCC
ENDILGKYIEKLWGDSSSSMKESSVTKEKLFNNGFL
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFTGIIEELGEVSRLQKREKSFKISIRASLILEDMKLGDSISTNGLCLTVSEFNQKEFTA
CCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEEHHHHCCCCCCCCCEEEEEECCCCHHHHH
DIMPESLNRSNLGTLNVGAKVNLERAMPLNGRFGGHIVSGHIDGVGQILEVKKEENAIWF
HCCCCCCCCCCCCEEECCCEEEHHHCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCEEEE
EIKTSLNLMRYTVEKGSITIDGISLTVAQVKSKSFSVSIIPHTLKETIMADKKVGDTVNL
EEECCCEEEEEEEECCCEEEECEEEEEEECCCCCEEEEECCHHHHHHHHHCCCCCCCCCC
ENDILGKYIEKLWGDSSSSMKESSVTKEKLFNNGFL
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8522537 [H]