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The map label for this gene is grpE

Identifier: 15672935

GI number: 15672935

Start: 977851

End: 978390

Strand: Direct

Name: grpE

Synonym: L0273

Alternate gene names: 15672935

Gene position: 977851-978390 (Clockwise)

Preceding gene: 15672934

Following gene: 15672936

Centisome position: 41.34

GC content: 38.33

Gene sequence:

>540_bases
ATGTCCGAAGAAACTAAAGAAGAAATCAAAAATGAAAAAGTTGATGAAGAAGTGACTGAGGAGTTGACAGAGGAAGCACT
TGAGGATATTGTTGAAGAAGAAATCAACGAACTTGATGAAGCACAAAAGTTAGCGACTGAGTGGGAAAATAAATTCCTTC
GCGTTTCTGCAGAAATGCAAAACGTACAACGTCGTGGAAATGAAGAACGTTTGCAACTTATCAAATATCGTTCACAAGAT
TTAGCAAAAAAAATCTTATCTTCACTAGATAATCTTGAACGTGCACTTGCTGTTGAAGGATTAACGGATGATGTTAAAAA
AGGCCTTGAAATGGTCCAAGAAAGCTTGATTTCAGCGCTTAAAGAAGAAGGCGTTGAAGAAGTTTCTTATGAAAGTTTCG
ACCATAATATTCACATGGCTGTTCAAACTGTCCCAGCTGATGATGAACATCCAGCTGACAGCATCGTGCAAGTTTTCCAA
AAAGGTTACCAACTTCACGAACGTTTACTTCGTCCAGCAATGGTTGTCGTAGCACAGTAA

Upstream 100 bases:

>100_bases
AAGTTTTAACTATGAAGCTGAGTGACTATTATCGTTATCTTGATGGTAACCATTATGAAATTAGTAAATGATAAAAAAGT
TATTTAAAAAAGGAGGGCCT

Downstream 100 bases:

>100_bases
ATTAAAAAATAAATTGACCGCAAAGTCTTTAAACTATTCATAAGTAATAAGAAATAAAGGAGATTTAATAATATGTCTAA
AATTATCGGTATTGACCTTG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MSEETKEEIKNEKVDEEVTEELTEEALEDIVEEEINELDEAQKLATEWENKFLRVSAEMQNVQRRGNEERLQLIKYRSQD
LAKKILSSLDNLERALAVEGLTDDVKKGLEMVQESLISALKEEGVEEVSYESFDHNIHMAVQTVPADDEHPADSIVQVFQ
KGYQLHERLLRPAMVVVAQ

Sequences:

>Translated_179_residues
MSEETKEEIKNEKVDEEVTEELTEEALEDIVEEEINELDEAQKLATEWENKFLRVSAEMQNVQRRGNEERLQLIKYRSQD
LAKKILSSLDNLERALAVEGLTDDVKKGLEMVQESLISALKEEGVEEVSYESFDHNIHMAVQTVPADDEHPADSIVQVFQ
KGYQLHERLLRPAMVVVAQ
>Mature_178_residues
SEETKEEIKNEKVDEEVTEELTEEALEDIVEEEINELDEAQKLATEWENKFLRVSAEMQNVQRRGNEERLQLIKYRSQDL
AKKILSSLDNLERALAVEGLTDDVKKGLEMVQESLISALKEEGVEEVSYESFDHNIHMAVQTVPADDEHPADSIVQVFQK
GYQLHERLLRPAMVVVAQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Escherichia coli, GI1788967, Length=149, Percent_Identity=32.2147651006711, Blast_Score=72, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324806, Length=181, Percent_Identity=28.7292817679558, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_LACLA (Q9CGY9)

Other databases:

- EMBL:   AE005176
- PIR:   A86744
- RefSeq:   NP_267109.1
- ProteinModelPortal:   Q9CGY9
- SMR:   Q9CGY9
- GeneID:   1114584
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0273
- NMPDR:   fig|272623.1.peg.976
- HOGENOM:   HBG732630
- OMA:   NVRRRME
- ProtClustDB:   PRK14156
- BioCyc:   LLAC272623:L0273-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 20581; Mature: 20450

Theoretical pI: Translated: 4.14; Mature: 4.14

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEETKEEIKNEKVDEEVTEELTEEALEDIVEEEINELDEAQKLATEWENKFLRVSAEMQ
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NVQRRGNEERLQLIKYRSQDLAKKILSSLDNLERALAVEGLTDDVKKGLEMVQESLISAL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KEEGVEEVSYESFDHNIHMAVQTVPADDEHPADSIVQVFQKGYQLHERLLRPAMVVVAQ
HHCCHHHHHHHHHCCHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEETKEEIKNEKVDEEVTEELTEEALEDIVEEEINELDEAQKLATEWENKFLRVSAEMQ
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NVQRRGNEERLQLIKYRSQDLAKKILSSLDNLERALAVEGLTDDVKKGLEMVQESLISAL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KEEGVEEVSYESFDHNIHMAVQTVPADDEHPADSIVQVFQKGYQLHERLLRPAMVVVAQ
HHCCHHHHHHHHHCCHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471