BacMap

Definition	Lactococcus lactis subsp. lactis Il1403, complete genome.
Accession	NC_002662
Length	2,365,589

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The map label for this gene is butA

Identifier: 15672880

GI number: 15672880

Start: 918527

End: 919288

Strand: Direct

Name: butA

Synonym: L118271

Alternate gene names: 15672880

Gene position: 918527-919288 (Clockwise)

Preceding gene: 30024012

Following gene: 15672881

Centisome position: 38.83

GC content: 36.75

Gene sequence:

>762_bases
ATGTCTAAAATTGCAGCAGTTACTGGAGCAGGTCAAGGAATAGGTTTTGCAATCGCAAAACGTCTCTATAATGATGGTTT
TAAAGTAGCTATTATTGATTACAACGAAGAAACAGCTCAAAAAGCAGCAAAAGAACTTGGAGAAAACTCATTTGCGATTA
AAGCTGATGTTTCCGACCGTGAACAAGTTATTTCAGCTTTGAATGCTGTTGTCGACAAATTTGGTGATTTAAATGTTGTA
GTTAATAATGCCGGAATTGCCCCAACAACACAAATTGAAACAATTACACCTGAACAATTTCATCAGGTCTATAATATTAA
TGTTGGTGGAGTTTTGTGGGGGACACAAACATCAACAGCACTTTTTAGAAAATTAGGTCATGGAGGTAAAATTATCAACG
CAACCTCACAAGCTGGAGTTGTTGGAAATCCAAATCTAATGCTTTATTCTTCTTCAAAATTTGCTGTTCGTGGGATGACT
CAAATTGCTGCTCGTGATTTAGCAGAAGAAGGAATCACAGTTAATGCTTATGCACCAGGGATTGTAAAAACACCAATGAT
GTTTGATATTGCGCATCAAGTTGGTAAAAATGCTGGTAAAGATGATGAATGGGGAATGCAAACTTTCGCAAAAGATATTG
CCATGAAACGTTTATCTGAGCCTGAAGATGTGGCAAATGTTGTTTCATTCTTAGCTGGCCCTGATTCAAACTATATTACC
GGTCAAACAATTATTGTTGATGGAGGAATGCAATTCCATTAA

Upstream 100 bases:

>100_bases
GGATTCGATACGCTGATTCATCATAATGAAACAGCTGTTAAAATTTTAGTTTCACCAACTGGTAAAGGTCTATAAAATAA
ATAAATAAAGGAGAAATAAG

Downstream 100 bases:

>100_bases
TCTAAAAATGAACTGTCAAGTTTTTATCTTGACAGTTCATTTTATTTTATGCTATAATTGTCTTAAATAAATGAACGAGG
TAAGGTCTATTGAAAATCTA

Product: acetoin reductase

Products: NA

Alternate protein names: Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSKIAAVTGAGQGIGFAIAKRLYNDGFKVAIIDYNEETAQKAAKELGENSFAIKADVSDREQVISALNAVVDKFGDLNVV
VNNAGIAPTTQIETITPEQFHQVYNINVGGVLWGTQTSTALFRKLGHGGKIINATSQAGVVGNPNLMLYSSSKFAVRGMT
QIAARDLAEEGITVNAYAPGIVKTPMMFDIAHQVGKNAGKDDEWGMQTFAKDIAMKRLSEPEDVANVVSFLAGPDSNYIT
GQTIIVDGGMQFH

Sequences:

>Translated_253_residues
MSKIAAVTGAGQGIGFAIAKRLYNDGFKVAIIDYNEETAQKAAKELGENSFAIKADVSDREQVISALNAVVDKFGDLNVV
VNNAGIAPTTQIETITPEQFHQVYNINVGGVLWGTQTSTALFRKLGHGGKIINATSQAGVVGNPNLMLYSSSKFAVRGMT
QIAARDLAEEGITVNAYAPGIVKTPMMFDIAHQVGKNAGKDDEWGMQTFAKDIAMKRLSEPEDVANVVSFLAGPDSNYIT
GQTIIVDGGMQFH
>Mature_252_residues
SKIAAVTGAGQGIGFAIAKRLYNDGFKVAIIDYNEETAQKAAKELGENSFAIKADVSDREQVISALNAVVDKFGDLNVVV
NNAGIAPTTQIETITPEQFHQVYNINVGGVLWGTQTSTALFRKLGHGGKIINATSQAGVVGNPNLMLYSSSKFAVRGMTQ
IAARDLAEEGITVNAYAPGIVKTPMMFDIAHQVGKNAGKDDEWGMQTFAKDIAMKRLSEPEDVANVVSFLAGPDSNYITG
QTIIVDGGMQFH

Specific function: Catalyzes the irreversible reduction of 2,3-butanediol to (S)-acetoin in the presence of NADH [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI15277342, Length=260, Percent_Identity=35.7692307692308, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI32483357, Length=254, Percent_Identity=31.496062992126, Blast_Score=120, Evalue=8e-28,
Organism=Homo sapiens, GI40254992, Length=255, Percent_Identity=32.5490196078431, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI19923817, Length=264, Percent_Identity=30.3030303030303, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI5031737, Length=260, Percent_Identity=30.7692307692308, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI4758504, Length=262, Percent_Identity=30.1526717557252, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI31542939, Length=211, Percent_Identity=34.1232227488152, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI59889578, Length=234, Percent_Identity=29.9145299145299, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI126723750, Length=256, Percent_Identity=27.734375, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI10190704, Length=253, Percent_Identity=27.2727272727273, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI224922801, Length=169, Percent_Identity=35.5029585798817, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI142976729, Length=189, Percent_Identity=32.8042328042328, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI83715985, Length=262, Percent_Identity=28.2442748091603, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI7705925, Length=246, Percent_Identity=29.2682926829268, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI210032110, Length=190, Percent_Identity=32.1052631578947, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI4503817, Length=187, Percent_Identity=28.3422459893048, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI66933014, Length=229, Percent_Identity=29.6943231441048, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI109715829, Length=239, Percent_Identity=27.1966527196653, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI33667109, Length=196, Percent_Identity=30.6122448979592, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI22507405, Length=191, Percent_Identity=28.7958115183246, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4503301, Length=225, Percent_Identity=25.7777777777778, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI44680136, Length=187, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI44680133, Length=187, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI17738292, Length=187, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI40807363, Length=192, Percent_Identity=26.5625, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI19743808, Length=189, Percent_Identity=26.4550264550265, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI126723191, Length=182, Percent_Identity=28.021978021978, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI87082100, Length=253, Percent_Identity=34.3873517786561, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1789057, Length=255, Percent_Identity=31.3725490196078, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1787335, Length=248, Percent_Identity=33.4677419354839, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788459, Length=256, Percent_Identity=29.296875, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI2367175, Length=257, Percent_Identity=31.9066147859922, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1789208, Length=248, Percent_Identity=30.6451612903226, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789378, Length=251, Percent_Identity=31.8725099601594, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790717, Length=249, Percent_Identity=29.3172690763052, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI2367365, Length=262, Percent_Identity=28.6259541984733, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787905, Length=250, Percent_Identity=30, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786812, Length=245, Percent_Identity=30.2040816326531, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87082160, Length=250, Percent_Identity=27.2, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787820, Length=230, Percent_Identity=25.6521739130435, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787526, Length=254, Percent_Identity=25.5905511811024, Blast_Score=64, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=254, Percent_Identity=33.4645669291339, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17555706, Length=251, Percent_Identity=33.8645418326693, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25147288, Length=255, Percent_Identity=33.3333333333333, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17562906, Length=271, Percent_Identity=32.4723247232472, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17531453, Length=260, Percent_Identity=32.3076923076923, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17560676, Length=253, Percent_Identity=28.4584980237154, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17562908, Length=261, Percent_Identity=31.8007662835249, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17559104, Length=259, Percent_Identity=33.2046332046332, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17560332, Length=267, Percent_Identity=31.8352059925094, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17538486, Length=261, Percent_Identity=34.0996168582375, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI193204405, Length=271, Percent_Identity=33.210332103321, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17538182, Length=262, Percent_Identity=29.3893129770992, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI115534694, Length=246, Percent_Identity=32.1138211382114, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17560220, Length=259, Percent_Identity=33.2046332046332, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71994604, Length=255, Percent_Identity=28.2352941176471, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17551412, Length=266, Percent_Identity=32.7067669172932, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17560150, Length=263, Percent_Identity=28.8973384030418, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17562904, Length=259, Percent_Identity=30.5019305019305, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17538480, Length=271, Percent_Identity=30.9963099630996, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17567345, Length=191, Percent_Identity=31.9371727748691, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17562990, Length=265, Percent_Identity=29.4339622641509, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17544670, Length=266, Percent_Identity=26.6917293233083, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994600, Length=257, Percent_Identity=26.8482490272374, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI72000259, Length=261, Percent_Identity=28.735632183908, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17565030, Length=264, Percent_Identity=31.4393939393939, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17562910, Length=262, Percent_Identity=25.1908396946565, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17508651, Length=262, Percent_Identity=28.2442748091603, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17561272, Length=243, Percent_Identity=27.5720164609054, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17536025, Length=259, Percent_Identity=27.4131274131274, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17563726, Length=264, Percent_Identity=26.8939393939394, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17536651, Length=268, Percent_Identity=28.3582089552239, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32563809, Length=200, Percent_Identity=28.5, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17507613, Length=200, Percent_Identity=28.5, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=256, Percent_Identity=27.734375, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323882, Length=257, Percent_Identity=25.6809338521401, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24639444, Length=252, Percent_Identity=32.9365079365079, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI21355319, Length=252, Percent_Identity=34.5238095238095, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI28571526, Length=258, Percent_Identity=32.5581395348837, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17737361, Length=264, Percent_Identity=32.9545454545455, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24644337, Length=234, Percent_Identity=30.3418803418803, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21357041, Length=254, Percent_Identity=31.1023622047244, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24644339, Length=254, Percent_Identity=31.1023622047244, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI23397609, Length=252, Percent_Identity=31.7460317460317, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281364892, Length=172, Percent_Identity=33.1395348837209, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24640492, Length=190, Percent_Identity=31.0526315789474, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281364494, Length=244, Percent_Identity=29.0983606557377, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24643142, Length=247, Percent_Identity=27.5303643724696, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI116007236, Length=197, Percent_Identity=26.3959390862944, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571387, Length=200, Percent_Identity=28, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21356301, Length=187, Percent_Identity=28.8770053475936, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24663911, Length=200, Percent_Identity=28, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24641232, Length=203, Percent_Identity=28.0788177339901, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24641388, Length=202, Percent_Identity=30.1980198019802, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24648693, Length=198, Percent_Identity=29.2929292929293, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21355889, Length=198, Percent_Identity=29.2929292929293, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI116007238, Length=177, Percent_Identity=26.5536723163842, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355631, Length=165, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24640442, Length=202, Percent_Identity=31.1881188118812, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24649181, Length=189, Percent_Identity=26.4550264550265, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21357673, Length=186, Percent_Identity=27.9569892473118, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221510726, Length=197, Percent_Identity=30.9644670050761, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI116007234, Length=202, Percent_Identity=28.7128712871287, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014007
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.304 [H]

Molecular weight: Translated: 26972; Mature: 26841

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIAAVTGAGQGIGFAIAKRLYNDGFKVAIIDYNEETAQKAAKELGENSFAIKADVSDR
CCCEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEECCCHH
EQVISALNAVVDKFGDLNVVVNNAGIAPTTQIETITPEQFHQVYNINVGGVLWGTQTSTA
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCHHHHHHHEECCCCEEEECCCHHHH
LFRKLGHGGKIINATSQAGVVGNPNLMLYSSSKFAVRGMTQIAARDLAEEGITVNAYAPG
HHHHHCCCCEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCEEEEEECCC
IVKTPMMFDIAHQVGKNAGKDDEWGMQTFAKDIAMKRLSEPEDVANVVSFLAGPDSNYIT
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEC
GQTIIVDGGMQFH
CEEEEEECCCCCC
>Mature Secondary Structure 
SKIAAVTGAGQGIGFAIAKRLYNDGFKVAIIDYNEETAQKAAKELGENSFAIKADVSDR
CCEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEECCCHH
EQVISALNAVVDKFGDLNVVVNNAGIAPTTQIETITPEQFHQVYNINVGGVLWGTQTSTA
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCHHHHHHHEECCCCEEEECCCHHHH
LFRKLGHGGKIINATSQAGVVGNPNLMLYSSSKFAVRGMTQIAARDLAEEGITVNAYAPG
HHHHHCCCCEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCEEEEEECCC
IVKTPMMFDIAHQVGKNAGKDDEWGMQTFAKDIAMKRLSEPEDVANVVSFLAGPDSNYIT
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEC
GQTIIVDGGMQFH
CEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA