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The map label for this gene is gshR

Identifier: 15672828

GI number: 15672828

Start: 863576

End: 864883

Strand: Direct

Name: gshR

Synonym: L0197

Alternate gene names: 15672828

Gene position: 863576-864883 (Clockwise)

Preceding gene: 15672827

Following gene: 15672829

Centisome position: 36.51

GC content: 35.78

Gene sequence:

>1308_bases
ATGTTTGATTATATTATTATTGGTGCCGGACCCGGTGGACTAGGATTAGCTTATCGTTTAAAGACTAAAGATAACAAAAT
CGGAATTATTGAGAATGATAAATGGGGAGGAACTTGTCCAAACTATGGTTGTGACCCAACAAAAATGATGATGGCAGTTG
TCGAAGCCAAAAGTCGTGTCGAACAGTTAAAAGGGCAGGGTATTTCTGGTGAATTAAACATTGATTGGAAAGGGCTGAAA
AGTCGTAAGCTAAATATTACTGACCCTTATGAAAAATCAACTTTTACTGGTTTGAAAAATGCTGGAATAGAAACAATATA
TGGCAGTGCCGCTTTTAATGACCAAGGAAAGCTTGAAGTTGAAGGGAAAACTTATCAAGCTAAAACTTACATTATCGCAA
CAGGAAGCCGTCCACGTCTTTTAGATATTGATGGAAAAGAATTTTTAAAAACAAGTAATGATTTTTTAGCGCTTGAAGAA
TTTCCCGCTCAAATCAGCTTTTTAGGGAGTGGGCCAATTTCATTAGAACTGGCTCAAATTGCTAAAGCGGCTGGAAGCGA
TGTTACTATTATTTCACGTAAAAAAGCGAGAGTTGCTCATTTTGATGAAGAAATGGGGCAAGAATTTATTAATTACCTTA
AAGCTCAAGGTATTAAATTTATCGAGGATATTTCTGTAGATAAAGTCGAAAAAGTTGCTGACGGATTTTTACTGACTGAC
GGGACTGATTTTGAACATAAAACAGATTTAGTGATTGCGGGAGTAGGCCGTCAGCCAAATAGTGATAAGCTAAATTTAGA
AAAAGTAGGTGTCGAAACGGATGCTAAAGGTATCAAGGTAAACGAATATTTACAAACAAGCAATTCTAAAATTTATGCGA
TGGGAGATGTACTTTCTAAAAATCAACCTCATTTGACGCCTGTTTCTTCTTTTGAAGGAGCTTATTTAGGTGAAAATTTA
GTTAAGGATGAACCGCAAAAAATTGCTTATCCAGCAATTCCTACAATCATTTTTGGAACGGCAAAACTGGCTGAGGTTGG
CCATTTGGCAGGTGAGGGGATTCATACCAAGACTTTGGATTTAAGTTCTTGGTATACTTATAAAAGAATTAATGACCCCT
TGGCAAAATTAAAAGTTGCACTCAATGAAAAGCGAGAAATCGTGGGGGCATCAACGGTAAGTTCAGTGGCTGATGAAGTG
ATTAATTTAATTAATATTTTAATTCAACAAAAAATGACTTTGGCGGATGTTGAAAAAATGATTTTCACTTATCCAACAGT
GGCTAGTGATTTAGAATATTTTTACTGA

Upstream 100 bases:

>100_bases
CAAAATAGTTTATTATTTTAGAGAAAAAAAGTAGAATTAAGCTAAAGTGTTGTATCATGAGTACATTTTAAAGATCTATT
AATAATTAGGAGGCTAAAGA

Downstream 100 bases:

>100_bases
CAGAAAATAAATTACTACCGACATTATAATAGTAGAAACAATAAGTTACGGCAGAATAAATTTTTTACTGACAGACTTTA
TTTTCAAGCTGTCAGTAAAT

Product: glutathione reductase

Products: NA

Alternate protein names: GR; GRase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MFDYIIIGAGPGGLGLAYRLKTKDNKIGIIENDKWGGTCPNYGCDPTKMMMAVVEAKSRVEQLKGQGISGELNIDWKGLK
SRKLNITDPYEKSTFTGLKNAGIETIYGSAAFNDQGKLEVEGKTYQAKTYIIATGSRPRLLDIDGKEFLKTSNDFLALEE
FPAQISFLGSGPISLELAQIAKAAGSDVTIISRKKARVAHFDEEMGQEFINYLKAQGIKFIEDISVDKVEKVADGFLLTD
GTDFEHKTDLVIAGVGRQPNSDKLNLEKVGVETDAKGIKVNEYLQTSNSKIYAMGDVLSKNQPHLTPVSSFEGAYLGENL
VKDEPQKIAYPAIPTIIFGTAKLAEVGHLAGEGIHTKTLDLSSWYTYKRINDPLAKLKVALNEKREIVGASTVSSVADEV
INLINILIQQKMTLADVEKMIFTYPTVASDLEYFY

Sequences:

>Translated_435_residues
MFDYIIIGAGPGGLGLAYRLKTKDNKIGIIENDKWGGTCPNYGCDPTKMMMAVVEAKSRVEQLKGQGISGELNIDWKGLK
SRKLNITDPYEKSTFTGLKNAGIETIYGSAAFNDQGKLEVEGKTYQAKTYIIATGSRPRLLDIDGKEFLKTSNDFLALEE
FPAQISFLGSGPISLELAQIAKAAGSDVTIISRKKARVAHFDEEMGQEFINYLKAQGIKFIEDISVDKVEKVADGFLLTD
GTDFEHKTDLVIAGVGRQPNSDKLNLEKVGVETDAKGIKVNEYLQTSNSKIYAMGDVLSKNQPHLTPVSSFEGAYLGENL
VKDEPQKIAYPAIPTIIFGTAKLAEVGHLAGEGIHTKTLDLSSWYTYKRINDPLAKLKVALNEKREIVGASTVSSVADEV
INLINILIQQKMTLADVEKMIFTYPTVASDLEYFY
>Mature_435_residues
MFDYIIIGAGPGGLGLAYRLKTKDNKIGIIENDKWGGTCPNYGCDPTKMMMAVVEAKSRVEQLKGQGISGELNIDWKGLK
SRKLNITDPYEKSTFTGLKNAGIETIYGSAAFNDQGKLEVEGKTYQAKTYIIATGSRPRLLDIDGKEFLKTSNDFLALEE
FPAQISFLGSGPISLELAQIAKAAGSDVTIISRKKARVAHFDEEMGQEFINYLKAQGIKFIEDISVDKVEKVADGFLLTD
GTDFEHKTDLVIAGVGRQPNSDKLNLEKVGVETDAKGIKVNEYLQTSNSKIYAMGDVLSKNQPHLTPVSSFEGAYLGENL
VKDEPQKIAYPAIPTIIFGTAKLAEVGHLAGEGIHTKTLDLSSWYTYKRINDPLAKLKVALNEKREIVGASTVSSVADEV
INLINILIQQKMTLADVEKMIFTYPTVASDLEYFY

Specific function: Maintains high levels of reduced glutathione in the cytosol [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI50301238, Length=457, Percent_Identity=30.1969365426696, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI291045266, Length=322, Percent_Identity=30.1242236024845, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI291045268, Length=314, Percent_Identity=29.9363057324841, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI22035672, Length=434, Percent_Identity=26.7281105990783, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI33519430, Length=440, Percent_Identity=28.4090909090909, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI33519428, Length=440, Percent_Identity=28.4090909090909, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI33519426, Length=440, Percent_Identity=28.4090909090909, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI148277065, Length=440, Percent_Identity=28.4090909090909, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI148277071, Length=440, Percent_Identity=28.4090909090909, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI91199540, Length=467, Percent_Identity=26.7665952890792, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1789915, Length=420, Percent_Identity=28.5714285714286, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87082354, Length=450, Percent_Identity=25.7777777777778, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1786307, Length=445, Percent_Identity=25.8426966292135, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=24.6153846153846, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789065, Length=244, Percent_Identity=28.2786885245902, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71983429, Length=446, Percent_Identity=29.8206278026906, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71983419, Length=446, Percent_Identity=29.8206278026906, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17557007, Length=468, Percent_Identity=26.9230769230769, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI32565766, Length=460, Percent_Identity=25.8695652173913, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71982272, Length=436, Percent_Identity=25.4587155963303, Blast_Score=105, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6325166, Length=464, Percent_Identity=25.8620689655172, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321091, Length=461, Percent_Identity=24.9457700650759, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325240, Length=487, Percent_Identity=21.5605749486653, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640549, Length=464, Percent_Identity=27.1551724137931, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24640553, Length=464, Percent_Identity=27.1551724137931, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24640551, Length=464, Percent_Identity=27.1551724137931, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI17737741, Length=469, Percent_Identity=26.226012793177, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21358499, Length=456, Percent_Identity=26.5350877192982, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: 650 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.7 [H]

Molecular weight: Translated: 47733; Mature: 47733

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDYIIIGAGPGGLGLAYRLKTKDNKIGIIENDKWGGTCPNYGCDPTKMMMAVVEAKSRV
CCEEEEEECCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EQLKGQGISGELNIDWKGLKSRKLNITDPYEKSTFTGLKNAGIETIYGSAAFNDQGKLEV
HHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHCCHHCCCCEEEECCEEECCCCEEEE
EGKTYQAKTYIIATGSRPRLLDIDGKEFLKTSNDFLALEEFPAQISFLGSGPISLELAQI
CCCEEEEEEEEEEECCCCEEEECCCHHHHHCCCCEEEEECCCCEEEEECCCCCEEEHHHH
AKAAGSDVTIISRKKARVAHFDEEMGQEFINYLKAQGIKFIEDISVDKVEKVADGFLLTD
HHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHCCEEEEC
GTDFEHKTDLVIAGVGRQPNSDKLNLEKVGVETDAKGIKVNEYLQTSNSKIYAMGDVLSK
CCCCCCCCCEEEEECCCCCCCCCCCCEEECCCCCCCCCEEHHHHHCCCCEEEEECHHHCC
NQPHLTPVSSFEGAYLGENLVKDEPQKIAYPAIPTIIFGTAKLAEVGHLAGEGIHTKTLD
CCCCCCCCCCCCCCHHCCHHHCCCCCCEECCCCCHHEECCHHHHHHHHHCCCCCEEEEEE
LSSWYTYKRINDPLAKLKVALNEKREIVGASTVSSVADEVINLINILIQQKMTLADVEKM
CCCCEEHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
IFTYPTVASDLEYFY
HHHCCCHHHHHHHCC
>Mature Secondary Structure
MFDYIIIGAGPGGLGLAYRLKTKDNKIGIIENDKWGGTCPNYGCDPTKMMMAVVEAKSRV
CCEEEEEECCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EQLKGQGISGELNIDWKGLKSRKLNITDPYEKSTFTGLKNAGIETIYGSAAFNDQGKLEV
HHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHCCHHCCCCEEEECCEEECCCCEEEE
EGKTYQAKTYIIATGSRPRLLDIDGKEFLKTSNDFLALEEFPAQISFLGSGPISLELAQI
CCCEEEEEEEEEEECCCCEEEECCCHHHHHCCCCEEEEECCCCEEEEECCCCCEEEHHHH
AKAAGSDVTIISRKKARVAHFDEEMGQEFINYLKAQGIKFIEDISVDKVEKVADGFLLTD
HHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHCCEEEEC
GTDFEHKTDLVIAGVGRQPNSDKLNLEKVGVETDAKGIKVNEYLQTSNSKIYAMGDVLSK
CCCCCCCCCEEEEECCCCCCCCCCCCEEECCCCCCCCCEEHHHHHCCCCEEEEECHHHCC
NQPHLTPVSSFEGAYLGENLVKDEPQKIAYPAIPTIIFGTAKLAEVGHLAGEGIHTKTLD
CCCCCCCCCCCCCCHHCCHHHCCCCCCEECCCCCHHEECCHHHHHHHHHCCCCCEEEEEE
LSSWYTYKRINDPLAKLKVALNEKREIVGASTVSSVADEVINLINILIQQKMTLADVEKM
CCCCEEHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
IFTYPTVASDLEYFY
HHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1849605; 10984043 [H]