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The map label for this gene is yhgA

Identifier: 15672735

GI number: 15672735

Start: 759951

End: 760679

Strand: Direct

Name: yhgA

Synonym: L159954

Alternate gene names: 15672735

Gene position: 759951-760679 (Clockwise)

Preceding gene: 15672734

Following gene: 15672736

Centisome position: 32.13

GC content: 36.35

Gene sequence:

>729_bases
ATGACTGAAACAAATGAAACGATTGATTTAATGATGAATCACACTTCGGTCAGAAATTTTACAGAAGAAGCAATTGAGCC
TAAAGATTTGGAAAAAATCTTAAAAGCTGCTCAAATGGCATCAACATGGAAAAATTTCCAATCATATTCTGTGATTGTTG
TAGAATCACAAGCTCAAAAAGATGCGATTTATGAATTTCAACCACAAAAATCAATCAAAAATTGTGCAGCATATTTAGTT
TTTGTTGGCGATTTAAATCGTGCTCAAAAGGCAGTCGAATTGGATCAAGGCAATTTCCAACCGCAAGGAATTGAAAGCTT
ATTGATTACATCAGTTGATGCCGCAACAGCTGGACAAAATGCGCTTCTTGCAGCAGAATCTTTAGGCTACAACGGGGTGA
TGGTTGGCTTAATTCGAGACCAATCCTCAGAAATTTCTAAAGTCTTGAACTTGCCAGATTATACTTATCCTATTTTTGGC
ATTGCACTCGGTAAGGCCGCACGTTTGAACAAAGTAAAACCACGTCTTCCTTTGGAAGCAACAGCTTTTAAAGAAAAATA
TGTTGAGCAAACGTCTGAAACCATTGAAAAATATGACCAAGTTCAAGAAGAATATGCTGGAAATAGACGCTTAAACAAAT
GGTCTGAACGGATTGTTGACCAGTGGGGCCAACCAGAAATTTCAGCTTCAACAGAAAATTTAAAGGCGAAAAAATTACTG
TTTAATTAA

Upstream 100 bases:

>100_bases
GAAACTAAGCGAGCTGATGAAATTTGGCCAGCAAAACGTGTTATGGAACGCGTGAAATATTTGGCAGAAGAAATGCGATT
AGAGGGGACGAATAGACGTC

Downstream 100 bases:

>100_bases
AGAAAATCTTAGAACCGAAACTTCCAGCCACCTATCAAGTAGCCACAAGTCAAGATTTTATTGATGGAGAAATTTATCAA
GCAGAATTTATAGATAAAAC

Product: oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MTETNETIDLMMNHTSVRNFTEEAIEPKDLEKILKAAQMASTWKNFQSYSVIVVESQAQKDAIYEFQPQKSIKNCAAYLV
FVGDLNRAQKAVELDQGNFQPQGIESLLITSVDAATAGQNALLAAESLGYNGVMVGLIRDQSSEISKVLNLPDYTYPIFG
IALGKAARLNKVKPRLPLEATAFKEKYVEQTSETIEKYDQVQEEYAGNRRLNKWSERIVDQWGQPEISASTENLKAKKLL
FN

Sequences:

>Translated_242_residues
MTETNETIDLMMNHTSVRNFTEEAIEPKDLEKILKAAQMASTWKNFQSYSVIVVESQAQKDAIYEFQPQKSIKNCAAYLV
FVGDLNRAQKAVELDQGNFQPQGIESLLITSVDAATAGQNALLAAESLGYNGVMVGLIRDQSSEISKVLNLPDYTYPIFG
IALGKAARLNKVKPRLPLEATAFKEKYVEQTSETIEKYDQVQEEYAGNRRLNKWSERIVDQWGQPEISASTENLKAKKLL
FN
>Mature_241_residues
TETNETIDLMMNHTSVRNFTEEAIEPKDLEKILKAAQMASTWKNFQSYSVIVVESQAQKDAIYEFQPQKSIKNCAAYLVF
VGDLNRAQKAVELDQGNFQPQGIESLLITSVDAATAGQNALLAAESLGYNGVMVGLIRDQSSEISKVLNLPDYTYPIFGI
ALGKAARLNKVKPRLPLEATAFKEKYVEQTSETIEKYDQVQEEYAGNRRLNKWSERIVDQWGQPEISASTENLKAKKLLF
N

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=217, Percent_Identity=33.1797235023041, Blast_Score=114, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 27177; Mature: 27046

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETNETIDLMMNHTSVRNFTEEAIEPKDLEKILKAAQMASTWKNFQSYSVIVVESQAQK
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
DAIYEFQPQKSIKNCAAYLVFVGDLNRAQKAVELDQGNFQPQGIESLLITSVDAATAGQN
CCHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHCCCC
ALLAAESLGYNGVMVGLIRDQSSEISKVLNLPDYTYPIFGIALGKAARLNKVKPRLPLEA
CEEEHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHCCHHHHCCCCCCCCCHH
TAFKEKYVEQTSETIEKYDQVQEEYAGNRRLNKWSERIVDQWGQPEISASTENLKAKKLL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHC
FN
CC
>Mature Secondary Structure 
TETNETIDLMMNHTSVRNFTEEAIEPKDLEKILKAAQMASTWKNFQSYSVIVVESQAQK
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
DAIYEFQPQKSIKNCAAYLVFVGDLNRAQKAVELDQGNFQPQGIESLLITSVDAATAGQN
CCHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHCCCC
ALLAAESLGYNGVMVGLIRDQSSEISKVLNLPDYTYPIFGIALGKAARLNKVKPRLPLEA
CEEEHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHCCHHHHCCCCCCCCCHH
TAFKEKYVEQTSETIEKYDQVQEEYAGNRRLNKWSERIVDQWGQPEISASTENLKAKKLL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHC
FN
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA