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The map label for this gene is yhdB

Identifier: 15672709

GI number: 15672709

Start: 731781

End: 732884

Strand: Reverse

Name: yhdB

Synonym: L131805

Alternate gene names: 15672709

Gene position: 732884-731781 (Counterclockwise)

Preceding gene: 30024008

Following gene: 15672708

Centisome position: 30.98

GC content: 36.68

Gene sequence:

>1104_bases
ATGATAATTGAAAAAATTACAATGTTTCATGTTCAACTCCCTATGAAATTTAATTTTAAGACCGCCAAAGGTTCCTTAAA
TCTCCGAGATACAATTATTATAAAAGTTGAAAGTCCTGACGGACTTTCGGGATTTGGTGAAGTTGTTGCTTTCACGACTC
CTTTTTATACTTCAGAAACTTTTGCTGATTCTTGGAAAATCTTGGAAGAAACTTACCTGCCAGAAATTTTGCAAAAAGAA
TTCTCACACCCTTTTGAAATACATGAATTTTTTAGAAATCCCTTACCAATGGCACTGGCCGGACTAGAAAATGCACTTCT
AGATTTGTATTTTAAAGAAAGAAATAAAAATTTGATTGCTGGTCTTTTCCAAGAAAAATTAGCTGATAAAATCCCACGTG
GTGCGGTGCTTGGACAAATGTCTGACGAGCAAACCATTAAGGAAATTGACCAACTTATTAACAGTGGCGTCAAAAGAATT
AAATTAAAAATTAGTCCACAAATTGGGACTGACTTGATTAAAAAGCTGGTAAAAAGATATCCACAAATTACATTTGCTCT
TGATGCCAATCAAAGTTTTCAACTGACAGACTGGCCAGTCATCAAAGAGCTAGACGAACTTGGTCTGGCTTGTATCGAAG
AACCTTTTGATATCAAAGATTTATCTGAATTAAAATTACTGACAGAAAACTTCTTAACACCAATTTGCTTTGACGAATCC
GTTCAAGATTTAGAAAGTCTAAAAATACTGACAGAACTTCCTTTTCAAACGATGCTCAATGTAAAAATAGGTCGTTTAGG
TGGACTTTATCAAACGCAAAAAGCTATCGAATTTTGCAGACAGCATCAGATTGGCTTTTGGATTGGCAGTATGGTTGAGT
CTGGTATCTCAAAAATACTCCATGTCCAACTCGCCGCGCTTTCTGGAAATGCTATGGCCGGTGATTTATCCGATTCTAAG
CATTATTTTGACCTAGACCTCGTTCAACCAGAAATTGCATTCCCAAATGGCTGGATGACCGTCCCGACTGGCATTGGCAT
CGGTCTGTCAGTTAATGAAGTCGCGCTAGATCACTATACTGTAAATAAGGAGATTTTCCAATGA

Upstream 100 bases:

>100_bases
CTGAAAAACTGGCAAAATACAAACGACCACAATCCATCACTTTCATGGATGAATTGCCCAAAAATGCTTCTGGAAAAATC
TTGAGAAAAGAGCTTAAAGG

Downstream 100 bases:

>100_bases
ACATGATTGACCAACTCAATATCACAGATTTTCAAGTTTTTACTGACGAGAATTCTGACAAGTCTGTCAGTAAAATTTAT
AAGTTCTCAAGTAAAATGAT

Product: racemase

Products: NA

Alternate protein names: OSB synthase; OSBS; 4-(2'-carboxyphenyl)-4-oxybutyric acid synthase; o-succinylbenzoic acid synthase [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MIIEKITMFHVQLPMKFNFKTAKGSLNLRDTIIIKVESPDGLSGFGEVVAFTTPFYTSETFADSWKILEETYLPEILQKE
FSHPFEIHEFFRNPLPMALAGLENALLDLYFKERNKNLIAGLFQEKLADKIPRGAVLGQMSDEQTIKEIDQLINSGVKRI
KLKISPQIGTDLIKKLVKRYPQITFALDANQSFQLTDWPVIKELDELGLACIEEPFDIKDLSELKLLTENFLTPICFDES
VQDLESLKILTELPFQTMLNVKIGRLGGLYQTQKAIEFCRQHQIGFWIGSMVESGISKILHVQLAALSGNAMAGDLSDSK
HYFDLDLVQPEIAFPNGWMTVPTGIGIGLSVNEVALDHYTVNKEIFQ

Sequences:

>Translated_367_residues
MIIEKITMFHVQLPMKFNFKTAKGSLNLRDTIIIKVESPDGLSGFGEVVAFTTPFYTSETFADSWKILEETYLPEILQKE
FSHPFEIHEFFRNPLPMALAGLENALLDLYFKERNKNLIAGLFQEKLADKIPRGAVLGQMSDEQTIKEIDQLINSGVKRI
KLKISPQIGTDLIKKLVKRYPQITFALDANQSFQLTDWPVIKELDELGLACIEEPFDIKDLSELKLLTENFLTPICFDES
VQDLESLKILTELPFQTMLNVKIGRLGGLYQTQKAIEFCRQHQIGFWIGSMVESGISKILHVQLAALSGNAMAGDLSDSK
HYFDLDLVQPEIAFPNGWMTVPTGIGIGLSVNEVALDHYTVNKEIFQ
>Mature_367_residues
MIIEKITMFHVQLPMKFNFKTAKGSLNLRDTIIIKVESPDGLSGFGEVVAFTTPFYTSETFADSWKILEETYLPEILQKE
FSHPFEIHEFFRNPLPMALAGLENALLDLYFKERNKNLIAGLFQEKLADKIPRGAVLGQMSDEQTIKEIDQLINSGVKRI
KLKISPQIGTDLIKKLVKRYPQITFALDANQSFQLTDWPVIKELDELGLACIEEPFDIKDLSELKLLTENFLTPICFDES
VQDLESLKILTELPFQTMLNVKIGRLGGLYQTQKAIEFCRQHQIGFWIGSMVESGISKILHVQLAALSGNAMAGDLSDSK
HYFDLDLVQPEIAFPNGWMTVPTGIGIGLSVNEVALDHYTVNKEIFQ

Specific function: Converts SHCHC to OSB [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=338, Percent_Identity=23.6686390532544, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354
- InterPro:   IPR010197 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.113 [H]

Molecular weight: Translated: 41492; Mature: 41492

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIEKITMFHVQLPMKFNFKTAKGSLNLRDTIIIKVESPDGLSGFGEVVAFTTPFYTSET
CCEEEEEEEEEEECEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCH
FADSWKILEETYLPEILQKEFSHPFEIHEFFRNPLPMALAGLENALLDLYFKERNKNLIA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
GLFQEKLADKIPRGAVLGQMSDEQTIKEIDQLINSGVKRIKLKISPQIGTDLIKKLVKRY
HHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHC
PQITFALDANQSFQLTDWPVIKELDELGLACIEEPFDIKDLSELKLLTENFLTPICFDES
CCEEEEEECCCCEECCCCHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHCCCCEECCHH
VQDLESLKILTELPFQTMLNVKIGRLGGLYQTQKAIEFCRQHQIGFWIGSMVESGISKIL
HHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
HVQLAALSGNAMAGDLSDSKHYFDLDLVQPEIAFPNGWMTVPTGIGIGLSVNEVALDHYT
HHHHEEECCCCEECCCCCCCEEEEEEEECCCEECCCCCEECCCCCCCCCCHHHEEEEEEE
VNKEIFQ
CCHHHCC
>Mature Secondary Structure
MIIEKITMFHVQLPMKFNFKTAKGSLNLRDTIIIKVESPDGLSGFGEVVAFTTPFYTSET
CCEEEEEEEEEEECEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCH
FADSWKILEETYLPEILQKEFSHPFEIHEFFRNPLPMALAGLENALLDLYFKERNKNLIA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
GLFQEKLADKIPRGAVLGQMSDEQTIKEIDQLINSGVKRIKLKISPQIGTDLIKKLVKRY
HHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHC
PQITFALDANQSFQLTDWPVIKELDELGLACIEEPFDIKDLSELKLLTENFLTPICFDES
CCEEEEEECCCCEECCCCHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHCCCCEECCHH
VQDLESLKILTELPFQTMLNVKIGRLGGLYQTQKAIEFCRQHQIGFWIGSMVESGISKIL
HHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
HVQLAALSGNAMAGDLSDSKHYFDLDLVQPEIAFPNGWMTVPTGIGIGLSVNEVALDHYT
HHHHEEECCCCEECCCCCCCEEEEEEEECCCEECCCCCEECCCCCCCCCCHHHEEEEEEE
VNKEIFQ
CCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10194342 [H]