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The map label for this gene is menE

Identifier: 30024008

GI number: 30024008

Start: 732881

End: 734236

Strand: Reverse

Name: menE

Synonym: L0172

Alternate gene names: 30024008

Gene position: 734236-732881 (Counterclockwise)

Preceding gene: 15672711

Following gene: 15672709

Centisome position: 31.04

GC content: 38.27

Gene sequence:

>1356_bases
ATGAAATGGTTAAAAAAACAGGCGGAACTTTATCCCCAAAAACAATTTTTAAATGACTCTACTTTTGCCCAAATCAATCA
GGAAGTCAATAAGATGGCTGAACATTTGGCCCCCCTTATTGACAATCAATCACGGGTTGCGCTCCTTTCAGAAAACTCTG
TTGAAATGGCAGTCGTTTTGTTTGCCTTGCTCGGTTTGTCAAAAGAGGTTCTTCTTTTGAATAGGCATCTGACCGAATAC
GAACTGGCTGACCAAATCAAGGAACTCAAAATTGACAAAGTCTTTACTTCGGATTTACTGACAGAAAAAGTTACTGACAG
CATTTCTTTTTCGGAAATTTGGACTAGTAACCCTTGCCCTGTAAGTCTGTCAGCAGATTTTCCTGATGAAAAAATTGCAG
TCATCATGAACACTTCCGCTACTACCGGAAAATTTAAATCTGTCCCTATTACTTGGGGCATGATTTCAAATCACGTCAAA
GCCTCAAAGGAAACGCTTGGCGCTTATGACAATGATAATTGGCTTGTCATCTTACCTATGTTTCATGTCTCTGGGCTTTC
CATTATTATGCGTACTTTATATAATGCAACATCAGCGACCGTTGTTGATAAATTTGATGAAAACCAACTTCTTGAAATGA
TCAATTCTGGGAAAATCAACATGGTTTCACTCGTCCCAACCCTCCTTACAAGAATTGCTGACAAATTGCATAGCAATAAC
TTACGTTTAATTCTACTTGGTGGTGAATTTATTCCCCAACCTTTAATCAAAAAATGCCAAGAATTAGGTCTTCCTATTTA
CAAAACTTATGGAATGACTGAAAGTTTTTCACAATCTGTCACTTTTAATATTTTAGATTTCCCTGATAAAACAAGCTCTG
TCGGGCGGCCTTTACCAGGTGTTGAAATTGAGATTCGTCAAGCTGACCTTGCTGGCGTTGGTGAAATTTGGTTAAAATCT
CCGATGTTGATGAAAGCTTACCTAGGCCAAAAGCCTTACGGCACAGCTTTTGAAACAGGCGATATTGGTTATCTTGATAC
GGACGGTTTTCTCTATCTGCTCAATCGCAGAAAAGATATCATTATTTCTGGCGGTGAAAATATTTACCCCAAAGAAATCG
AAGATTTAGTTTATTCTCTTCCTGAAATCAAGGAATGTGCACTTGTTGCTAAGCCAGATGCCAAGTGGGGACAAGTGCCA
ATTCTCTTCGTTTCAGGCAATATTTCCCAAGAAAAATTAGAAAACTTTCTCACTGAAAAACTGGCAAAATACAAACGACC
ACAATCCATCACTTTCATGGATGAATTGCCCAAAAATGCTTCTGGAAAAATCTTGAGAAAAGAGCTTAAAGGATGA

Upstream 100 bases:

>100_bases
TCTTTTTGACGCTTTTTGTGATTTAAAAGTTACTGACAGGATTTCTGTCAGTAAAAAATACACAGAAAGGAGAAAATCGA
GTTCGCTCACGTCTTAAAAT

Downstream 100 bases:

>100_bases
TAATTGAAAAAATTACAATGTTTCATGTTCAACTCCCTATGAAATTTAATTTTAAGACCGCCAAAGGTTCCTTAAATCTC
CGAGATACAATTATTATAAA

Product: O-succinylbenzoic acid-CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MKWLKKQAELYPQKQFLNDSTFAQINQEVNKMAEHLAPLIDNQSRVALLSENSVEMAVVLFALLGLSKEVLLLNRHLTEY
ELADQIKELKIDKVFTSDLLTEKVTDSISFSEIWTSNPCPVSLSADFPDEKIAVIMNTSATTGKFKSVPITWGMISNHVK
ASKETLGAYDNDNWLVILPMFHVSGLSIIMRTLYNATSATVVDKFDENQLLEMINSGKINMVSLVPTLLTRIADKLHSNN
LRLILLGGEFIPQPLIKKCQELGLPIYKTYGMTESFSQSVTFNILDFPDKTSSVGRPLPGVEIEIRQADLAGVGEIWLKS
PMLMKAYLGQKPYGTAFETGDIGYLDTDGFLYLLNRRKDIIISGGENIYPKEIEDLVYSLPEIKECALVAKPDAKWGQVP
ILFVSGNISQEKLENFLTEKLAKYKRPQSITFMDELPKNASGKILRKELKG

Sequences:

>Translated_451_residues
MKWLKKQAELYPQKQFLNDSTFAQINQEVNKMAEHLAPLIDNQSRVALLSENSVEMAVVLFALLGLSKEVLLLNRHLTEY
ELADQIKELKIDKVFTSDLLTEKVTDSISFSEIWTSNPCPVSLSADFPDEKIAVIMNTSATTGKFKSVPITWGMISNHVK
ASKETLGAYDNDNWLVILPMFHVSGLSIIMRTLYNATSATVVDKFDENQLLEMINSGKINMVSLVPTLLTRIADKLHSNN
LRLILLGGEFIPQPLIKKCQELGLPIYKTYGMTESFSQSVTFNILDFPDKTSSVGRPLPGVEIEIRQADLAGVGEIWLKS
PMLMKAYLGQKPYGTAFETGDIGYLDTDGFLYLLNRRKDIIISGGENIYPKEIEDLVYSLPEIKECALVAKPDAKWGQVP
ILFVSGNISQEKLENFLTEKLAKYKRPQSITFMDELPKNASGKILRKELKG
>Mature_451_residues
MKWLKKQAELYPQKQFLNDSTFAQINQEVNKMAEHLAPLIDNQSRVALLSENSVEMAVVLFALLGLSKEVLLLNRHLTEY
ELADQIKELKIDKVFTSDLLTEKVTDSISFSEIWTSNPCPVSLSADFPDEKIAVIMNTSATTGKFKSVPITWGMISNHVK
ASKETLGAYDNDNWLVILPMFHVSGLSIIMRTLYNATSATVVDKFDENQLLEMINSGKINMVSLVPTLLTRIADKLHSNN
LRLILLGGEFIPQPLIKKCQELGLPIYKTYGMTESFSQSVTFNILDFPDKTSSVGRPLPGVEIEIRQADLAGVGEIWLKS
PMLMKAYLGQKPYGTAFETGDIGYLDTDGFLYLLNRRKDIIISGGENIYPKEIEDLVYSLPEIKECALVAKPDAKWGQVP
ILFVSGNISQEKLENFLTEKLAKYKRPQSITFMDELPKNASGKILRKELKG

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA)

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily

Homologues:

Organism=Homo sapiens, GI187761345, Length=387, Percent_Identity=27.1317829457364, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI187761343, Length=387, Percent_Identity=27.1317829457364, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI156151445, Length=354, Percent_Identity=28.2485875706215, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI42544132, Length=279, Percent_Identity=28.3154121863799, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI38505220, Length=277, Percent_Identity=27.0758122743682, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI122937307, Length=237, Percent_Identity=30.379746835443, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI115511026, Length=126, Percent_Identity=35.7142857142857, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI157311624, Length=294, Percent_Identity=26.530612244898, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI157311622, Length=294, Percent_Identity=26.530612244898, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI58082049, Length=238, Percent_Identity=26.0504201680672, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI27477105, Length=179, Percent_Identity=31.2849162011173, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI13375727, Length=121, Percent_Identity=31.404958677686, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1788595, Length=337, Percent_Identity=30.5637982195846, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1788107, Length=514, Percent_Identity=25.875486381323, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI221142682, Length=488, Percent_Identity=25, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI145693145, Length=380, Percent_Identity=28.4210526315789, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786810, Length=491, Percent_Identity=24.6435845213849, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789201, Length=450, Percent_Identity=24.6666666666667, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786529, Length=371, Percent_Identity=25.3369272237197, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790505, Length=467, Percent_Identity=23.1263383297645, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=485, Percent_Identity=28.4536082474227, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17559526, Length=439, Percent_Identity=28.0182232346241, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI32563687, Length=443, Percent_Identity=29.1196388261851, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17557194, Length=490, Percent_Identity=25.1020408163265, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17531443, Length=362, Percent_Identity=27.6243093922652, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=494, Percent_Identity=24.2914979757085, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71985884, Length=346, Percent_Identity=28.0346820809249, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71996755, Length=297, Percent_Identity=29.2929292929293, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI25141359, Length=431, Percent_Identity=24.5939675174014, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=357, Percent_Identity=25.4901960784314, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=356, Percent_Identity=25.561797752809, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71994690, Length=355, Percent_Identity=25.0704225352113, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71994694, Length=355, Percent_Identity=25.0704225352113, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71994703, Length=355, Percent_Identity=25.0704225352113, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17556356, Length=334, Percent_Identity=27.5449101796407, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=360, Percent_Identity=30, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6319264, Length=289, Percent_Identity=25.9515570934256, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323182, Length=295, Percent_Identity=24.0677966101695, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355181, Length=461, Percent_Identity=28.1995661605206, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI20130357, Length=401, Percent_Identity=26.9326683291771, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24581924, Length=359, Percent_Identity=27.2980501392758, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI18859661, Length=387, Percent_Identity=25.0645994832041, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356947, Length=349, Percent_Identity=24.6418338108883, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24653035, Length=351, Percent_Identity=25.0712250712251, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI161076582, Length=355, Percent_Identity=25.0704225352113, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648260, Length=353, Percent_Identity=22.6628895184136, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19922652, Length=370, Percent_Identity=22.4324324324324, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648257, Length=359, Percent_Identity=23.1197771587744, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21355225, Length=367, Percent_Identity=26.9754768392371, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356441, Length=294, Percent_Identity=24.4897959183673, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24648255, Length=352, Percent_Identity=23.0113636363636, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648253, Length=352, Percent_Identity=23.0113636363636, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921316, Length=154, Percent_Identity=33.1168831168831, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281365686, Length=365, Percent_Identity=25.4794520547945, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21358303, Length=310, Percent_Identity=25.4838709677419, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MENE_LACLA (Q9CHK3)

Other databases:

- EMBL:   AE005176
- PIR:   H86715
- RefSeq:   NP_266884.2
- ProteinModelPortal:   Q9CHK3
- GeneID:   1114354
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0172
- NMPDR:   fig|272623.1.peg.747
- HOGENOM:   HBG547964
- OMA:   ENIAPSE
- ProtClustDB:   CLSK643928
- BioCyc:   LLAC272623:L0172-MONOMER
- BRENDA:   6.2.1.26
- HAMAP:   MF_00731
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015
- TIGRFAMs:   TIGR01923

Pfam domain/function: PF00501 AMP-binding

EC number: =6.2.1.26

Molecular weight: Translated: 50578; Mature: 50578

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWLKKQAELYPQKQFLNDSTFAQINQEVNKMAEHLAPLIDNQSRVALLSENSVEMAVVL
CCHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
FALLGLSKEVLLLNRHLTEYELADQIKELKIDKVFTSDLLTEKVTDSISFSEIWTSNPCP
HHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCC
VSLSADFPDEKIAVIMNTSATTGKFKSVPITWGMISNHVKASKETLGAYDNDNWLVILPM
EEEECCCCCCEEEEEEECCCCCCCCEECCEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
FHVSGLSIIMRTLYNATSATVVDKFDENQLLEMINSGKINMVSLVPTLLTRIADKLHSNN
HHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCC
LRLILLGGEFIPQPLIKKCQELGLPIYKTYGMTESFSQSVTFNILDFPDKTSSVGRPLPG
EEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCHHHCCCEEEEEEECCCCCCCCCCCCCC
VEIEIRQADLAGVGEIWLKSPMLMKAYLGQKPYGTAFETGDIGYLDTDGFLYLLNRRKDI
CEEEEEECCCCCHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCEEECCCEEEEEECCCEE
IISGGENIYPKEIEDLVYSLPEIKECALVAKPDAKWGQVPILFVSGNISQEKLENFLTEK
EEECCCCCCHHHHHHHHHCCCCHHHCEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHH
LAKYKRPQSITFMDELPKNASGKILRKELKG
HHHHCCCCCEEHHHHCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MKWLKKQAELYPQKQFLNDSTFAQINQEVNKMAEHLAPLIDNQSRVALLSENSVEMAVVL
CCHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
FALLGLSKEVLLLNRHLTEYELADQIKELKIDKVFTSDLLTEKVTDSISFSEIWTSNPCP
HHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCC
VSLSADFPDEKIAVIMNTSATTGKFKSVPITWGMISNHVKASKETLGAYDNDNWLVILPM
EEEECCCCCCEEEEEEECCCCCCCCEECCEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
FHVSGLSIIMRTLYNATSATVVDKFDENQLLEMINSGKINMVSLVPTLLTRIADKLHSNN
HHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCC
LRLILLGGEFIPQPLIKKCQELGLPIYKTYGMTESFSQSVTFNILDFPDKTSSVGRPLPG
EEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCHHHCCCEEEEEEECCCCCCCCCCCCCC
VEIEIRQADLAGVGEIWLKSPMLMKAYLGQKPYGTAFETGDIGYLDTDGFLYLLNRRKDI
CEEEEEECCCCCHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCEEECCCEEEEEECCCEE
IISGGENIYPKEIEDLVYSLPEIKECALVAKPDAKWGQVPILFVSGNISQEKLENFLTEK
EEECCCCCCHHHHHHHHHCCCCHHHCEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHH
LAKYKRPQSITFMDELPKNASGKILRKELKG
HHHHCCCCCEEHHHHCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471