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The map label for this gene is qor

Identifier: 15672699

GI number: 15672699

Start: 723093

End: 724079

Strand: Reverse

Name: qor

Synonym: L123116

Alternate gene names: 15672699

Gene position: 724079-723093 (Counterclockwise)

Preceding gene: 15672704

Following gene: 15672697

Centisome position: 30.61

GC content: 35.87

Gene sequence:

>987_bases
ATGAAAGCAATTGGAACTATTGATTCTAGCAAAAATTTTATTGACTTTGAAATAGAAAAGCCAATCTTAAGACCCCATGA
TTTATTGATAAAAGTCGAGGCAATCTCAATCAATCCAGTTGATACAAAGGTTAGAAAAGGGATTAAAGGAAATTTAGCAG
AACCAAAAATTCTAGGTTGGGATGGTTTAGGAACTGTCGTCGAACTTGGGAGTGAAACTAAGCTTTTTAAGGTTGGGGAT
AAAGTGTTTTGGGCCGGTGATGTTACTCGTTCAGGTTCAAATGCTGAATTCCAAGCCGTTGATGAACGAATTGTTGGTTT
TGCTCCCAAAAATTTGACCAAAGAAAAAGCAGTCGCTATGCCTCTAACAAGTTTGACTGCCTATGAACTTCTCTTTGAAA
AACTAGAAGTGACTGATAAATCTAAAGGAAAATCACTCCTCATTATTAATGGTGCAGGTGGTGTTGGCTCAGTAGCAATC
CAAATGGCCAAAAATGCTGGATTGACAGTGATTGCTACGGCATCAAATCCCCAAGCCATTGAATGGGTCAAAAATTTTGG
CGCCGATTATACCGTCAATCATCATGAAAAATTGGTTCCTCAAGTTCATGAACTTGGCTTTAAGTTTGTAGATTATATTT
TAATTTTAAATGCCGTTGTTGAACATATGCCAGCAGTAAATGAGTTAATTGCGCCACAAGGTTCGATTGCAAATATTGTT
GAACCAGGGCAAGCTATTAATTTAGATCAACTAGCTCATAAATCTGCACGTTTTAACTTTGAATGGATGTTTACAAAAGT
CGTCTCAAATACATCTGATTTACAAAGTCAACATGATATTTTAAATAAAGTTTCTGAATGGCTAGATAATGACGTTCTAA
AAAGTACAATGACTCAGAATCTCGGACCAATTACCGCTGAGAATGTGAAAAAAGCTCATGAACTCATTGAAGAAAATCAT
ACAATAGGGAAAATTATCCTATCTTAA

Upstream 100 bases:

>100_bases
AAATTTTTAATGACATGATGTAAATATTTAATATTAAATTCAAGTGATTGACTTAGGAGTCAGACTGGTATAAACTTTTA
CTTATAAGAGAGGTAGAGAG

Downstream 100 bases:

>100_bases
CGCTTAAAAAAATCCTCTTATTTGAGGATTTTTATATTTCATCAAAACGAACAATTAGATAAACGGCCCCTATCATGAAA
GTAGAGCCCATCATTGTCCA

Product: quinone oxidoreductase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKAIGTIDSSKNFIDFEIEKPILRPHDLLIKVEAISINPVDTKVRKGIKGNLAEPKILGWDGLGTVVELGSETKLFKVGD
KVFWAGDVTRSGSNAEFQAVDERIVGFAPKNLTKEKAVAMPLTSLTAYELLFEKLEVTDKSKGKSLLIINGAGGVGSVAI
QMAKNAGLTVIATASNPQAIEWVKNFGADYTVNHHEKLVPQVHELGFKFVDYILILNAVVEHMPAVNELIAPQGSIANIV
EPGQAINLDQLAHKSARFNFEWMFTKVVSNTSDLQSQHDILNKVSEWLDNDVLKSTMTQNLGPITAENVKKAHELIEENH
TIGKIILS

Sequences:

>Translated_328_residues
MKAIGTIDSSKNFIDFEIEKPILRPHDLLIKVEAISINPVDTKVRKGIKGNLAEPKILGWDGLGTVVELGSETKLFKVGD
KVFWAGDVTRSGSNAEFQAVDERIVGFAPKNLTKEKAVAMPLTSLTAYELLFEKLEVTDKSKGKSLLIINGAGGVGSVAI
QMAKNAGLTVIATASNPQAIEWVKNFGADYTVNHHEKLVPQVHELGFKFVDYILILNAVVEHMPAVNELIAPQGSIANIV
EPGQAINLDQLAHKSARFNFEWMFTKVVSNTSDLQSQHDILNKVSEWLDNDVLKSTMTQNLGPITAENVKKAHELIEENH
TIGKIILS
>Mature_328_residues
MKAIGTIDSSKNFIDFEIEKPILRPHDLLIKVEAISINPVDTKVRKGIKGNLAEPKILGWDGLGTVVELGSETKLFKVGD
KVFWAGDVTRSGSNAEFQAVDERIVGFAPKNLTKEKAVAMPLTSLTAYELLFEKLEVTDKSKGKSLLIINGAGGVGSVAI
QMAKNAGLTVIATASNPQAIEWVKNFGADYTVNHHEKLVPQVHELGFKFVDYILILNAVVEHMPAVNELIAPQGSIANIV
EPGQAINLDQLAHKSARFNFEWMFTKVVSNTSDLQSQHDILNKVSEWLDNDVLKSTMTQNLGPITAENVKKAHELIEENH
TIGKIILS

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=350, Percent_Identity=26.2857142857143, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI194239676, Length=181, Percent_Identity=32.5966850828729, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI194239674, Length=181, Percent_Identity=32.5966850828729, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI13236495, Length=181, Percent_Identity=32.5966850828729, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI22538446, Length=189, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI22538444, Length=189, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI28557745, Length=200, Percent_Identity=29, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI18379349, Length=190, Percent_Identity=28.9473684210526, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1790485, Length=184, Percent_Identity=32.0652173913043, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17507255, Length=243, Percent_Identity=31.6872427983539, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71987554, Length=229, Percent_Identity=28.82096069869, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319520, Length=204, Percent_Identity=31.3725490196078, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR014182
- InterPro:   IPR011032
- InterPro:   IPR016040
- InterPro:   IPR002364 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 35912; Mature: 35912

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01162 QOR_ZETA_CRYSTAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIGTIDSSKNFIDFEIEKPILRPHDLLIKVEAISINPVDTKVRKGIKGNLAEPKILGW
CCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEEEECCCHHHHHCCCCCCCCCCEEECC
DGLGTVVELGSETKLFKVGDKVFWAGDVTRSGSNAEFQAVDERIVGFAPKNLTKEKAVAM
CCCCHHHHCCCCCEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHC
PLTSLTAYELLFEKLEVTDKSKGKSLLIINGAGGVGSVAIQMAKNAGLTVIATASNPQAI
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCHHH
EWVKNFGADYTVNHHEKLVPQVHELGFKFVDYILILNAVVEHMPAVNELIAPQGSIANIV
HHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHC
EPGQAINLDQLAHKSARFNFEWMFTKVVSNTSDLQSQHDILNKVSEWLDNDVLKSTMTQN
CCCCCCCHHHHHHHHHCCCCEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LGPITAENVKKAHELIEENHTIGKIILS
CCCCCHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MKAIGTIDSSKNFIDFEIEKPILRPHDLLIKVEAISINPVDTKVRKGIKGNLAEPKILGW
CCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEEEECCCHHHHHCCCCCCCCCCEEECC
DGLGTVVELGSETKLFKVGDKVFWAGDVTRSGSNAEFQAVDERIVGFAPKNLTKEKAVAM
CCCCHHHHCCCCCEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHC
PLTSLTAYELLFEKLEVTDKSKGKSLLIINGAGGVGSVAIQMAKNAGLTVIATASNPQAI
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCHHH
EWVKNFGADYTVNHHEKLVPQVHELGFKFVDYILILNAVVEHMPAVNELIAPQGSIANIV
HHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHC
EPGQAINLDQLAHKSARFNFEWMFTKVVSNTSDLQSQHDILNKVSEWLDNDVLKSTMTQN
CCCCCCCHHHHHHHHHCCCCEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LGPITAENVKKAHELIEENHTIGKIILS
CCCCCHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.26 {1,4-naphthoquinone}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]