Click here to switch to the map view.

The map label for this gene is apu

Identifier: 15672683

GI number: 15672683

Start: 702393

End: 704195

Strand: Direct

Name: apu

Synonym: L102412

Alternate gene names: 15672683

Gene position: 702393-704195 (Clockwise)

Preceding gene: 15672682

Following gene: 15672684

Centisome position: 29.69

GC content: 32.5

Gene sequence:

>1803_bases
ATGTATTATTATAATCCGTGGAATTTAGATTATAAACAACCTTTTGGGGCCATCAAGGTTGGAAACTCAATGAGCTTGAG
ATTTGCAACTGACCAACCTTCTGTACTTGTGACATGTGTAATTCGACGTGATTTTGGAAAACGTTATGAATTTTCTATGA
CCAAAGATTCAGGCGGAGATTTTAGACTTACCATTCCTTTTGATGAAGAGCCAGGACTTTATTTTTATCATTTTGAAATA
GTCGAGTCTTCGGATTTGGGAGAAACTAGGCGATTTTATGGTTGTTCTGGCATAGGAGGAGAGGGGCTTCTATATACTGA
TGAAAATGATGTTAAACCTTATCAGCTGACTGTATTTGAAAAAGAAGATCAAGCTCCCAGCTGGTATCGTGAAGCAGTTT
TCTATCAAATTTTCCCAGATCGTTTCTATAATGGAAATGAAAATGGTCAAATCAATCACCCAAAACCCAATTCATTTATC
TATGGAAGAAAAACGGATAATCCTTTCTATGTTAAAGAAGAAAATGGTGATATTGCACGTTGGGATTTTTTCGGTGGAAA
TTTAAGAGGAATTATTAAAAAAATTCCCTACTTAAAAGAACTGGGAATCAATGCGATTTATCTTAATCCAATTTTTTCTG
GTACAAGTAACCATCGCTATGATACGAATGATTACCTAAAAATTGATTCAATGCTAGGTAGGCAGGAAGATTTTGAAGAA
TTGATTCAGTTGCTTCATCAAGAAAAAATGCATTTGATTTTGGATGGTGTTTTTTCTCATGTAGGAAAAAACTCGCTTTA
TTTCAATATTAATGGTGATTATGGCGATGATGAGGGAGCAGCAAAAAATCCTGATTCACCTTATTTTGATTGGTTTAAAT
TTGAAAATTATCCTTTTGAGTATAAATCTTGGTGGGGAATTAAAGATTTACCTGAAATAGACAAAGATAATGATTCTTTT
CGCAATTTTATTTATGGTGAGAAAAATTCTGTTTTAGCTAAATGGAATGCCTTGGGTATTGATGGTTGGCGTCTGGATGT
TGCTGATGAACTCCCTGATTCATTTATTAAAGGAATTCGAGAAAATCTTGACTCTTATTCAGATAAGATATTGATTGGTG
AAATTTGGGAAGATGCTTCCAACAAAATTTCTTATGGAAAAAGAAGAAATTATATTCTTGGTGGAAGTTTGCAAGCTGCT
ATGAATTATCCGTTTCGTGATTTAATTATTAATTTATTAAATGGTCATCGTTCTAGTCAAGATGTCGCTCATCAATTGAT
GACTTTGCAAGAGAATTATCCTAAAGATATTTTTTATAATAATTTAAACAATCTAGGGACTCATGATACTGAACGAATTT
TGACAATGGTTGGAGAAAAGAATTTGTCAGTAGCGTTAGCAATGCTTTTTGTATTACCAGGAATTCATTGTATTTACTAT
GGAGATGAAGCAGGATTGTCAGGTGGCAAAGACCCTGAGAATCGTAAATATTTTCCTTGGGATAACATTTCACCCAATGT
TTACAATGTTTATAAAAGTTGGACGCAAAAACGTCTGTCAGAAAATAGCTTGAAATATGGAGAGTTTTCAACTTTTTTTA
CTGACAGGTTGTTAGGAATATTGCGATATACAGATTCAGAAGTTTTTGTTGAACTCATTAATCCGAGTGAAGATGAGCTT
AATATAGAAGTTCAAGAAATTACTTTTTTACAAAATCTGAAATTCTTACCAGAATTAAAAAAATTACTGACAGAAAAAGT
GATTAGTGGAAAAACAAATTTAGAATTAAAGATGAAAATTTAA

Upstream 100 bases:

>100_bases
AAAAAGAATGAAGAAATTTACTGACAGAAATTTTTGTTACTGACAGAGTAAATTTTAGGAGCCTTTAGCCTTAAGTGTTA
ATAAAGGAAATGAGAATTAG

Downstream 100 bases:

>100_bases
AACTTGCTGATGCAAGTTTTTTTACTGACAGATTTATTTGTCAGTAAAAAATGATAAAAACTATATTTTTTAGCTAAATC
TTATTTTAGAAAAGAAGAAA

Product: amylopullulanase

Products: NA

Alternate protein names: Alpha-amylase/pullulanase; Pullulanase type II; Alpha-amylase; 1,4-alpha-D-glucan glucanohydrolase; Pullulanase; 1,4-alpha-D-glucan glucanohydrolase; Alpha-dextrin endo-1,6-alpha-glucosidase [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MYYYNPWNLDYKQPFGAIKVGNSMSLRFATDQPSVLVTCVIRRDFGKRYEFSMTKDSGGDFRLTIPFDEEPGLYFYHFEI
VESSDLGETRRFYGCSGIGGEGLLYTDENDVKPYQLTVFEKEDQAPSWYREAVFYQIFPDRFYNGNENGQINHPKPNSFI
YGRKTDNPFYVKEENGDIARWDFFGGNLRGIIKKIPYLKELGINAIYLNPIFSGTSNHRYDTNDYLKIDSMLGRQEDFEE
LIQLLHQEKMHLILDGVFSHVGKNSLYFNINGDYGDDEGAAKNPDSPYFDWFKFENYPFEYKSWWGIKDLPEIDKDNDSF
RNFIYGEKNSVLAKWNALGIDGWRLDVADELPDSFIKGIRENLDSYSDKILIGEIWEDASNKISYGKRRNYILGGSLQAA
MNYPFRDLIINLLNGHRSSQDVAHQLMTLQENYPKDIFYNNLNNLGTHDTERILTMVGEKNLSVALAMLFVLPGIHCIYY
GDEAGLSGGKDPENRKYFPWDNISPNVYNVYKSWTQKRLSENSLKYGEFSTFFTDRLLGILRYTDSEVFVELINPSEDEL
NIEVQEITFLQNLKFLPELKKLLTEKVISGKTNLELKMKI

Sequences:

>Translated_600_residues
MYYYNPWNLDYKQPFGAIKVGNSMSLRFATDQPSVLVTCVIRRDFGKRYEFSMTKDSGGDFRLTIPFDEEPGLYFYHFEI
VESSDLGETRRFYGCSGIGGEGLLYTDENDVKPYQLTVFEKEDQAPSWYREAVFYQIFPDRFYNGNENGQINHPKPNSFI
YGRKTDNPFYVKEENGDIARWDFFGGNLRGIIKKIPYLKELGINAIYLNPIFSGTSNHRYDTNDYLKIDSMLGRQEDFEE
LIQLLHQEKMHLILDGVFSHVGKNSLYFNINGDYGDDEGAAKNPDSPYFDWFKFENYPFEYKSWWGIKDLPEIDKDNDSF
RNFIYGEKNSVLAKWNALGIDGWRLDVADELPDSFIKGIRENLDSYSDKILIGEIWEDASNKISYGKRRNYILGGSLQAA
MNYPFRDLIINLLNGHRSSQDVAHQLMTLQENYPKDIFYNNLNNLGTHDTERILTMVGEKNLSVALAMLFVLPGIHCIYY
GDEAGLSGGKDPENRKYFPWDNISPNVYNVYKSWTQKRLSENSLKYGEFSTFFTDRLLGILRYTDSEVFVELINPSEDEL
NIEVQEITFLQNLKFLPELKKLLTEKVISGKTNLELKMKI
>Mature_600_residues
MYYYNPWNLDYKQPFGAIKVGNSMSLRFATDQPSVLVTCVIRRDFGKRYEFSMTKDSGGDFRLTIPFDEEPGLYFYHFEI
VESSDLGETRRFYGCSGIGGEGLLYTDENDVKPYQLTVFEKEDQAPSWYREAVFYQIFPDRFYNGNENGQINHPKPNSFI
YGRKTDNPFYVKEENGDIARWDFFGGNLRGIIKKIPYLKELGINAIYLNPIFSGTSNHRYDTNDYLKIDSMLGRQEDFEE
LIQLLHQEKMHLILDGVFSHVGKNSLYFNINGDYGDDEGAAKNPDSPYFDWFKFENYPFEYKSWWGIKDLPEIDKDNDSF
RNFIYGEKNSVLAKWNALGIDGWRLDVADELPDSFIKGIRENLDSYSDKILIGEIWEDASNKISYGKRRNYILGGSLQAA
MNYPFRDLIINLLNGHRSSQDVAHQLMTLQENYPKDIFYNNLNNLGTHDTERILTMVGEKNLSVALAMLFVLPGIHCIYY
GDEAGLSGGKDPENRKYFPWDNISPNVYNVYKSWTQKRLSENSLKYGEFSTFFTDRLLGILRYTDSEVFVELINPSEDEL
NIEVQEITFLQNLKFLPELKKLLTEKVISGKTNLELKMKI

Specific function: May Play A Role In Regulating The Intracellular Level Of Maltotriose. Cleaves Glucose From The Reducing End Of Maltotriose And Longer Maltodextrins With A Chain Length Of Up To 7 Glucose Units. [C]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted, cell wall. Note=It C-terminus may serve as an S-layer anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 SLH (S-layer homology) domains [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=425, Percent_Identity=20.9411764705882, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1786604, Length=529, Percent_Identity=30.0567107750473, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1789995, Length=530, Percent_Identity=23.3962264150943, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790687, Length=418, Percent_Identity=23.2057416267943, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321726, Length=250, Percent_Identity=25.6, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322241, Length=250, Percent_Identity=24.8, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322021, Length=250, Percent_Identity=24.8, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324416, Length=250, Percent_Identity=24.8, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321731, Length=274, Percent_Identity=24.8175182481752, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6319776, Length=274, Percent_Identity=24.8175182481752, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6322245, Length=264, Percent_Identity=24.2424242424242, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24586593, Length=588, Percent_Identity=20.9183673469388, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24586587, Length=420, Percent_Identity=22.6190476190476, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24586591, Length=525, Percent_Identity=22.4761904761905, Blast_Score=82, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24586597, Length=418, Percent_Identity=22.7272727272727, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI221330053, Length=427, Percent_Identity=21.7798594847775, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013784
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR002044
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR001119 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N; PF00395 SLH [H]

EC number: =3.2.1.1; =3.2.1.41 [H]

Molecular weight: Translated: 69740; Mature: 69740

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYYNPWNLDYKQPFGAIKVGNSMSLRFATDQPSVLVTCVIRRDFGKRYEFSMTKDSGGD
CCEECCCCCCCCCCCCEEEECCCEEEEEECCCCCEEEEEEEECCCCCCEEEEEECCCCCC
FRLTIPFDEEPGLYFYHFEIVESSDLGETRRFYGCSGIGGEGLLYTDENDVKPYQLTVFE
EEEEEECCCCCCEEEEEEEEEECCCCCCHHHHCCCCCCCCCEEEEECCCCCCCEEEEEEE
KEDQAPSWYREAVFYQIFPDRFYNGNENGQINHPKPNSFIYGRKTDNPFYVKEENGDIAR
CCCCCCHHHHHHHEEEECCHHHCCCCCCCEECCCCCCCEEEEECCCCCEEEEECCCCEEE
WDFFGGNLRGIIKKIPYLKELGINAIYLNPIFSGTSNHRYDTNDYLKIDSMLGRQEDFEE
EEECCCCHHHHHHHCCHHHHCCCCEEEECCEECCCCCCCCCCCCCEEEHHHHCCHHHHHH
LIQLLHQEKMHLILDGVFSHVGKNSLYFNINGDYGDDEGAAKNPDSPYFDWFKFENYPFE
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCC
YKSWWGIKDLPEIDKDNDSFRNFIYGEKNSVLAKWNALGIDGWRLDVADELPDSFIKGIR
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEEEEEECCCCEEECCHHHCHHHHHHHHH
ENLDSYSDKILIGEIWEDASNKISYGKRRNYILGGSLQAAMNYPFRDLIINLLNGHRSSQ
HHHHHHCCEEEEEEHHCCCCCCCCCCCCCCEEECCCHHHHHCCCHHHHHHHHHCCCCCHH
DVAHQLMTLQENYPKDIFYNNLNNLGTHDTERILTMVGEKNLSVALAMLFVLPGIHCIYY
HHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEE
GDEAGLSGGKDPENRKYFPWDNISPNVYNVYKSWTQKRLSENSLKYGEFSTFFTDRLLGI
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LRYTDSEVFVELINPSEDELNIEVQEITFLQNLKFLPELKKLLTEKVISGKTNLELKMKI
HHCCCCEEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
>Mature Secondary Structure
MYYYNPWNLDYKQPFGAIKVGNSMSLRFATDQPSVLVTCVIRRDFGKRYEFSMTKDSGGD
CCEECCCCCCCCCCCCEEEECCCEEEEEECCCCCEEEEEEEECCCCCCEEEEEECCCCCC
FRLTIPFDEEPGLYFYHFEIVESSDLGETRRFYGCSGIGGEGLLYTDENDVKPYQLTVFE
EEEEEECCCCCCEEEEEEEEEECCCCCCHHHHCCCCCCCCCEEEEECCCCCCCEEEEEEE
KEDQAPSWYREAVFYQIFPDRFYNGNENGQINHPKPNSFIYGRKTDNPFYVKEENGDIAR
CCCCCCHHHHHHHEEEECCHHHCCCCCCCEECCCCCCCEEEEECCCCCEEEEECCCCEEE
WDFFGGNLRGIIKKIPYLKELGINAIYLNPIFSGTSNHRYDTNDYLKIDSMLGRQEDFEE
EEECCCCHHHHHHHCCHHHHCCCCEEEECCEECCCCCCCCCCCCCEEEHHHHCCHHHHHH
LIQLLHQEKMHLILDGVFSHVGKNSLYFNINGDYGDDEGAAKNPDSPYFDWFKFENYPFE
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCC
YKSWWGIKDLPEIDKDNDSFRNFIYGEKNSVLAKWNALGIDGWRLDVADELPDSFIKGIR
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEEEEEECCCCEEECCHHHCHHHHHHHHH
ENLDSYSDKILIGEIWEDASNKISYGKRRNYILGGSLQAAMNYPFRDLIINLLNGHRSSQ
HHHHHHCCEEEEEEHHCCCCCCCCCCCCCCEEECCCHHHHHCCCHHHHHHHHHCCCCCHH
DVAHQLMTLQENYPKDIFYNNLNNLGTHDTERILTMVGEKNLSVALAMLFVLPGIHCIYY
HHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEE
GDEAGLSGGKDPENRKYFPWDNISPNVYNVYKSWTQKRLSENSLKYGEFSTFFTDRLLGI
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LRYTDSEVFVELINPSEDELNIEVQEITFLQNLKFLPELKKLLTEKVISGKTNLELKMKI
HHCCCCEEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8195085 [H]