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The map label for this gene is ybaF

Identifier: 15672086

GI number: 15672086

Start: 105274

End: 106026

Strand: Direct

Name: ybaF

Synonym: L105256

Alternate gene names: 15672086

Gene position: 105274-106026 (Clockwise)

Preceding gene: 15672085

Following gene: 15672087

Centisome position: 4.45

GC content: 36.79

Gene sequence:

>753_bases
ATGGCCAACCAATATTTTGTATTTAAAAAATTACCGGAACTGGGCGAAGAATTTATCATTGAAAATCAAGCAGCAGCTCA
TCATATTTTCACTGTGATGCGGGCTAAAAGTGGTGACCAAATCCGTCTTGTTTTTACTGATGGTGAAATTGGGCTGTCAG
AAGTCATTTCGCCAAGTGACCAATCAGTGAAGCTGATAAAATTACTGACAGAGTCCTCAGAATTGCCTGTTCAAATTACA
GTAGCTGTAGGATTTCCTAAAGGAGACAAGCTCGATTTTATTGTTGAAAAATCAACAGAATTAGGAGCTACAGCCATTTG
GTCAGCACCTTTTAAAGCTTCTGTCAGTAAATGGGACAAGAAAAAACTGACAAAAAAACAAGAAAAATTGGAAAAAGTTG
CTCTAGGAGCAGCCGAACAATCACGCAGACAAATGATTCCAGAACTTCAACTTTTTGAGCAATTTACTGATTTACTTGAT
AAATTTTCTGATTTTGACCAAGTTCTTATCGCTTATGAAGAATCAGCAAAAATGGGTGAGAAAACAATGTTCAAACAAGC
ACTGACAGAAATGTCACTCAACCAAAAAGTACTTATCATTTTTGGACCAGAAGGCGGAATTAGTCCAGACGAAATCACAA
AATTTTCCGATTTAGGTGCAAAAATGATTGGCTTAGGGCCGCGAATTTTAAGAGCAGAAACGGCTCCATTATATGCCCTC
TCAAGTATTTCAGCTTATTTTGAATTATTATAA

Upstream 100 bases:

>100_bases
AAAGGCTTAAATAAAGTCGTTTATCTTTCAGTCAATGGAGAAGCACAATAAAGAAAAGATTAGATTCAAAACTTTATTCT
AAGAAATGATTGGAAAAAAT

Downstream 100 bases:

>100_bases
ATAAAGAAAAATTTTTAGGAAATCGATTATGAAAAAACGTGCACAACGTAACAAAAAACGAATTCGCTGGGCTTCGGTCC
TCACAGTTTTCGTTCTTTTA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MANQYFVFKKLPELGEEFIIENQAAAHHIFTVMRAKSGDQIRLVFTDGEIGLSEVISPSDQSVKLIKLLTESSELPVQIT
VAVGFPKGDKLDFIVEKSTELGATAIWSAPFKASVSKWDKKKLTKKQEKLEKVALGAAEQSRRQMIPELQLFEQFTDLLD
KFSDFDQVLIAYEESAKMGEKTMFKQALTEMSLNQKVLIIFGPEGGISPDEITKFSDLGAKMIGLGPRILRAETAPLYAL
SSISAYFELL

Sequences:

>Translated_250_residues
MANQYFVFKKLPELGEEFIIENQAAAHHIFTVMRAKSGDQIRLVFTDGEIGLSEVISPSDQSVKLIKLLTESSELPVQIT
VAVGFPKGDKLDFIVEKSTELGATAIWSAPFKASVSKWDKKKLTKKQEKLEKVALGAAEQSRRQMIPELQLFEQFTDLLD
KFSDFDQVLIAYEESAKMGEKTMFKQALTEMSLNQKVLIIFGPEGGISPDEITKFSDLGAKMIGLGPRILRAETAPLYAL
SSISAYFELL
>Mature_249_residues
ANQYFVFKKLPELGEEFIIENQAAAHHIFTVMRAKSGDQIRLVFTDGEIGLSEVISPSDQSVKLIKLLTESSELPVQITV
AVGFPKGDKLDFIVEKSTELGATAIWSAPFKASVSKWDKKKLTKKQEKLEKVALGAAEQSRRQMIPELQLFEQFTDLLDK
FSDFDQVLIAYEESAKMGEKTMFKQALTEMSLNQKVLIIFGPEGGISPDEITKFSDLGAKMIGLGPRILRAETAPLYALS
SISAYFELL

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=244, Percent_Identity=27.4590163934426, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27845; Mature: 27713

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANQYFVFKKLPELGEEFIIENQAAAHHIFTVMRAKSGDQIRLVFTDGEIGLSEVISPSD
CCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHCCCCC
QSVKLIKLLTESSELPVQITVAVGFPKGDKLDFIVEKSTELGATAIWSAPFKASVSKWDK
CHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHH
KKLTKKQEKLEKVALGAAEQSRRQMIPELQLFEQFTDLLDKFSDFDQVLIAYEESAKMGE
HHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KTMFKQALTEMSLNQKVLIIFGPEGGISPDEITKFSDLGAKMIGLGPRILRAETAPLYAL
HHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCHHHHCCCCHHEECCCCCHHHH
SSISAYFELL
HHHHHHHHCC
>Mature Secondary Structure 
ANQYFVFKKLPELGEEFIIENQAAAHHIFTVMRAKSGDQIRLVFTDGEIGLSEVISPSD
CCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHCCCCC
QSVKLIKLLTESSELPVQITVAVGFPKGDKLDFIVEKSTELGATAIWSAPFKASVSKWDK
CHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHH
KKLTKKQEKLEKVALGAAEQSRRQMIPELQLFEQFTDLLDKFSDFDQVLIAYEESAKMGE
HHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KTMFKQALTEMSLNQKVLIIFGPEGGISPDEITKFSDLGAKMIGLGPRILRAETAPLYAL
HHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCHHHHCCCCHHEECCCCCHHHH
SSISAYFELL
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]