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The map label for this gene is mesJ

Identifier: 15672001

GI number: 15672001

Start: 23878

End: 25149

Strand: Direct

Name: mesJ

Synonym: L1002

Alternate gene names: 15672001

Gene position: 23878-25149 (Clockwise)

Preceding gene: 15672000

Following gene: 15672002

Centisome position: 1.01

GC content: 32.0

Gene sequence:

>1272_bases
ATGACACCAGCTCAACGTAAATTTTTAAAAATAGTCAAAGATGAACACTATTTTGATAAAGATTCAAAAATATTATTGGC
TTTATCAGGTGGTAAAGACTCAATGACTCTTTTTAACTGGCTCTATGATTTAAAAGAAGTTCTCGGTATTGAACTGGGAC
TGGCTCACATTAATCATGGCTTACGAGAAGAGTCAAAGTTTGAAGAAATTGCTCTTCGAGAAATGGCAACAAAACTTAAA
GTACCAATTTATGTTGACAAGTTTACAGGAGAGTTTACAGAAAAAAATGCACGTGATTTCCGTTATCAGTTTTTTGAAAA
GCTAATGATAGGAGAAAATTACAATATTTTGCTGACAGCTCATCATCAAGGAGACCTTGTTGAAACGGTATTAATGCGTC
AAATTACAGGACGTCCTTTGAGAAGTCTTCAGGGCATTGCTGACCGTCAACCTTTTGCAGGTGGGCAATTAATCAGACCA
TTATTGAAATTTACAAAAGAAGAACTTGATGCACAAACCTATTATGAAGATTCAACAAATCAAGGACTAGATTATTTTAG
AAATAGAATACGTAATCAACTAATTCCAGAATTAAAAAAAGAAAATCCTCAATTTTCACAATCAATCAGTGATTTGAGCT
CGGAAATTAAAAAGGCGTTGGCAGTAATTAATCAAAAAATTTCGGAACTTGAAATTGTTGATGAAAAAATATCATCTAAA
AAATTCATCTCACAAACAAAAGAATTACAACATTTTATCTTACAAGCATTTTTTGCTCAATATCCAGAAATAGAAGTAAG
TAAGAAAAAATTTGCAGAATTACTTCATATTATCAATCGTCCTCAACAATATTTCGCAAAATTGAATAAGGAATTTTATT
TTGTGAAGACTAAAGATTTCTTTTATCTTGAAAAAATTCAACTTGAAAGGGAGAATTCAGTAGAGATTGTAAGTGAAAAT
CCGCAAGATGAATCATTTATGGAAGTTTATTTGCCTCTTGAAGGGGAAATTGAAATTCGTAAACGCCAGCCTGGGGATCA
AATTCTAATCAATGGTCATCATAAAAAATTACGCAAGTTTTTTATTGATAACAAAGTTCCATTAAAGGCCCGAGAGAATC
CACTTATCTTCGTTGATAAAAAACTCTATGCCATTGTCGGTCTTGCGTGTTCTGATTTGAGTAAAATGCTCAAAAATGAT
AAAATTAGAAGAATATTATGGGTCAAACCCAGTATAGGAGAGGAAATCAACGATGCTCGAAAAAAATCTTGA

Upstream 100 bases:

>100_bases
TTGTATTTGCAAGTAAACCATATCTTTTGGTAATTGAAACTGATGGGGCAACAGATGATGAAATAAAAGCAATTTCTCAA
GATGTTTATGGAGTTATGAA

Downstream 100 bases:

>100_bases
TAAGGCCATTGAAAAAGTATTAGTTTCAGAAGAAGAAATTATAGAAAAATCCAAAGAACTTGGAGAAATATTAACAAAAG
AATACGAAGGAAAAAATCCA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MTPAQRKFLKIVKDEHYFDKDSKILLALSGGKDSMTLFNWLYDLKEVLGIELGLAHINHGLREESKFEEIALREMATKLK
VPIYVDKFTGEFTEKNARDFRYQFFEKLMIGENYNILLTAHHQGDLVETVLMRQITGRPLRSLQGIADRQPFAGGQLIRP
LLKFTKEELDAQTYYEDSTNQGLDYFRNRIRNQLIPELKKENPQFSQSISDLSSEIKKALAVINQKISELEIVDEKISSK
KFISQTKELQHFILQAFFAQYPEIEVSKKKFAELLHIINRPQQYFAKLNKEFYFVKTKDFFYLEKIQLERENSVEIVSEN
PQDESFMEVYLPLEGEIEIRKRQPGDQILINGHHKKLRKFFIDNKVPLKARENPLIFVDKKLYAIVGLACSDLSKMLKND
KIRRILWVKPSIGEEINDARKKS

Sequences:

>Translated_423_residues
MTPAQRKFLKIVKDEHYFDKDSKILLALSGGKDSMTLFNWLYDLKEVLGIELGLAHINHGLREESKFEEIALREMATKLK
VPIYVDKFTGEFTEKNARDFRYQFFEKLMIGENYNILLTAHHQGDLVETVLMRQITGRPLRSLQGIADRQPFAGGQLIRP
LLKFTKEELDAQTYYEDSTNQGLDYFRNRIRNQLIPELKKENPQFSQSISDLSSEIKKALAVINQKISELEIVDEKISSK
KFISQTKELQHFILQAFFAQYPEIEVSKKKFAELLHIINRPQQYFAKLNKEFYFVKTKDFFYLEKIQLERENSVEIVSEN
PQDESFMEVYLPLEGEIEIRKRQPGDQILINGHHKKLRKFFIDNKVPLKARENPLIFVDKKLYAIVGLACSDLSKMLKND
KIRRILWVKPSIGEEINDARKKS
>Mature_422_residues
TPAQRKFLKIVKDEHYFDKDSKILLALSGGKDSMTLFNWLYDLKEVLGIELGLAHINHGLREESKFEEIALREMATKLKV
PIYVDKFTGEFTEKNARDFRYQFFEKLMIGENYNILLTAHHQGDLVETVLMRQITGRPLRSLQGIADRQPFAGGQLIRPL
LKFTKEELDAQTYYEDSTNQGLDYFRNRIRNQLIPELKKENPQFSQSISDLSSEIKKALAVINQKISELEIVDEKISSKK
FISQTKELQHFILQAFFAQYPEIEVSKKKFAELLHIINRPQQYFAKLNKEFYFVKTKDFFYLEKIQLERENSVEIVSENP
QDESFMEVYLPLEGEIEIRKRQPGDQILINGHHKKLRKFFIDNKVPLKARENPLIFVDKKLYAIVGLACSDLSKMLKNDK
IRRILWVKPSIGEEINDARKKS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=200, Percent_Identity=33, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_LACLA (Q9CJH4)

Other databases:

- EMBL:   AE005176
- PIR:   C86627
- RefSeq:   NP_266175.1
- ProteinModelPortal:   Q9CJH4
- SMR:   Q9CJH4
- GeneID:   1113618
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L1002
- NMPDR:   fig|272623.1.peg.20
- HOGENOM:   HBG697356
- OMA:   GHHKKLR
- ProtClustDB:   CLSK876678
- BioCyc:   LLAC272623:L1002-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 49575; Mature: 49444

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAQRKFLKIVKDEHYFDKDSKILLALSGGKDSMTLFNWLYDLKEVLGIELGLAHINHG
CCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
LREESKFEEIALREMATKLKVPIYVDKFTGEFTEKNARDFRYQFFEKLMIGENYNILLTA
CCHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHCCHHHHHHHHHHHHHHCCCCEEEEEE
HHQGDLVETVLMRQITGRPLRSLQGIADRQPFAGGQLIRPLLKFTKEELDAQTYYEDSTN
CCCCHHHHHHHHHHHCCCHHHHHHCHHCCCCCCCHHHHHHHHHHHHHHHCHHHHCCCCCC
QGLDYFRNRIRNQLIPELKKENPQFSQSISDLSSEIKKALAVINQKISELEIVDEKISSK
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFISQTKELQHFILQAFFAQYPEIEVSKKKFAELLHIINRPQQYFAKLNKEFYFVKTKDF
HHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHCCCHHHHHHHCCCEEEEEECCE
FYLEKIQLERENSVEIVSENPQDESFMEVYLPLEGEIEIRKRQPGDQILINGHHKKLRKF
EEEHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEECCCCCEEEECCHHHHHHHH
FIDNKVPLKARENPLIFVDKKLYAIVGLACSDLSKMLKNDKIRRILWVKPSIGEEINDAR
HCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHH
KKS
CCC
>Mature Secondary Structure 
TPAQRKFLKIVKDEHYFDKDSKILLALSGGKDSMTLFNWLYDLKEVLGIELGLAHINHG
CCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
LREESKFEEIALREMATKLKVPIYVDKFTGEFTEKNARDFRYQFFEKLMIGENYNILLTA
CCHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHCCHHHHHHHHHHHHHHCCCCEEEEEE
HHQGDLVETVLMRQITGRPLRSLQGIADRQPFAGGQLIRPLLKFTKEELDAQTYYEDSTN
CCCCHHHHHHHHHHHCCCHHHHHHCHHCCCCCCCHHHHHHHHHHHHHHHCHHHHCCCCCC
QGLDYFRNRIRNQLIPELKKENPQFSQSISDLSSEIKKALAVINQKISELEIVDEKISSK
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFISQTKELQHFILQAFFAQYPEIEVSKKKFAELLHIINRPQQYFAKLNKEFYFVKTKDF
HHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHCCCHHHHHHHCCCEEEEEECCE
FYLEKIQLERENSVEIVSENPQDESFMEVYLPLEGEIEIRKRQPGDQILINGHHKKLRKF
EEEHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEECCCCCEEEECCHHHHHHHH
FIDNKVPLKARENPLIFVDKKLYAIVGLACSDLSKMLKNDKIRRILWVKPSIGEEINDAR
HCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHH
KKS
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471