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The map label for this gene is dnaN

Identifier: 15671984

GI number: 15671984

Start: 1882

End: 3024

Strand: Direct

Name: dnaN

Synonym: L0275

Alternate gene names: 15671984

Gene position: 1882-3024 (Clockwise)

Preceding gene: 15671983

Following gene: 15671985

Centisome position: 0.08

GC content: 31.93

Gene sequence:

>1143_bases
ATGATTAAATTTTCAATTAATAAAAATGCGTTTCAAAATGCTTTGAGAATTACCAAACAAGCAATTGGTTCTAAAGTAAC
AATTCCTGCTTTAACAAAGTTAAAAATTGAAGTTGAAGAAAATGGAATTACTTTAATTGGTTCAAATGGTCAAATTTCAA
TCAAGAATTTTTTACCTGTTGATAATAAAGATGCAAGTATGCTTATTTCAGGAACGGGTTCAGTTTTATTAGAAGCTGCT
TTCTTTGAAAATGTGGTAAGTCAATTACCAGAAGTCACTCTTGAATTTACTGAAAAAGAACAAAAGCAAGTTTTGTTAAC
TTCTGGTAAGTCAGAAATTACTTTAAAAGGCCTGGATTCAGAAATCTATCCGCATTTACAAGAAATTTCCGAAGGCTCTT
CATTGAAAATGAAAGTTAAAGTACTTAAAGAAATTTTTACAGAAACAGTTTTTGCTGTAAGTACTCAAGAAAATCGTCCA
ATTTTTACAGGTGTTCATTTAGAAACTTTATCAACTGGAGAATTAAAAGCAGTCGCAACTGACTCACATCGGATGAGTCA
ACGTTTATTGCCTTTAGAAGATTCAGAATTAAAATTTGATGTTATTTTGCCAAGTAAATCAATCAATAGTTTTAAAAATG
TTTTCACAAATGATGAAGAAGAGATTGAAATTTTCATTAGTGGAAGCCAAATGCTTTTTAGAAATGAAACAATTAGTTAT
TATAGTCGTTTGATTGAAGGATCTTATCCTGATACTAATCGTTTAATTCCAAATGAAGCTGATTATACTTTAGATTTAGT
TTTTGATGCTGCGCAATTGCGTCATACAATGGATCGTGCGCGTTTGTTGACAGTAATGACAACTAATGGAACTGTAAAAT
TAACGGTTTCTGGTGATTCTGTAGTAACCACAGCCAACTCTCCTGAGGTAGGTTCTGTCCATGAAGAATTGACGGCCTTA
TCAAAAGAAGGAAATGACTTAGCAATCAGCTTTAATCCTGAATATTTGATTGATGCTTTGAAAGTGATTAAAGCACCAGA
AGTTCGTATTCGCTTTATTTCTAATGTTCGTCCGTTCACTTTACAGCCACGTAATGAAGAATCTGGATTTGTTCAATTGA
TTACACCAGTACGTACAAATTAG

Upstream 100 bases:

>100_bases
AAAAGCTTATACTTCTAAGTGGATTTGTCTTATACAGATTATCCACAGGACCTACTACTATTACTATTATATTTATAAAA
AGAAATTATAAAGGAATCTC

Downstream 100 bases:

>100_bases
TTTTACTGACAGCTCTTTTGTCAGCTTAGATTTGCTGACGAAAATGTCTGTCAGTAAATTTAGATTTTGTTATAATGGAC
TTAACGAAAGTTAGGTCTAT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MIKFSINKNAFQNALRITKQAIGSKVTIPALTKLKIEVEENGITLIGSNGQISIKNFLPVDNKDASMLISGTGSVLLEAA
FFENVVSQLPEVTLEFTEKEQKQVLLTSGKSEITLKGLDSEIYPHLQEISEGSSLKMKVKVLKEIFTETVFAVSTQENRP
IFTGVHLETLSTGELKAVATDSHRMSQRLLPLEDSELKFDVILPSKSINSFKNVFTNDEEEIEIFISGSQMLFRNETISY
YSRLIEGSYPDTNRLIPNEADYTLDLVFDAAQLRHTMDRARLLTVMTTNGTVKLTVSGDSVVTTANSPEVGSVHEELTAL
SKEGNDLAISFNPEYLIDALKVIKAPEVRIRFISNVRPFTLQPRNEESGFVQLITPVRTN

Sequences:

>Translated_380_residues
MIKFSINKNAFQNALRITKQAIGSKVTIPALTKLKIEVEENGITLIGSNGQISIKNFLPVDNKDASMLISGTGSVLLEAA
FFENVVSQLPEVTLEFTEKEQKQVLLTSGKSEITLKGLDSEIYPHLQEISEGSSLKMKVKVLKEIFTETVFAVSTQENRP
IFTGVHLETLSTGELKAVATDSHRMSQRLLPLEDSELKFDVILPSKSINSFKNVFTNDEEEIEIFISGSQMLFRNETISY
YSRLIEGSYPDTNRLIPNEADYTLDLVFDAAQLRHTMDRARLLTVMTTNGTVKLTVSGDSVVTTANSPEVGSVHEELTAL
SKEGNDLAISFNPEYLIDALKVIKAPEVRIRFISNVRPFTLQPRNEESGFVQLITPVRTN
>Mature_380_residues
MIKFSINKNAFQNALRITKQAIGSKVTIPALTKLKIEVEENGITLIGSNGQISIKNFLPVDNKDASMLISGTGSVLLEAA
FFENVVSQLPEVTLEFTEKEQKQVLLTSGKSEITLKGLDSEIYPHLQEISEGSSLKMKVKVLKEIFTETVFAVSTQENRP
IFTGVHLETLSTGELKAVATDSHRMSQRLLPLEDSELKFDVILPSKSINSFKNVFTNDEEEIEIFISGSQMLFRNETISY
YSRLIEGSYPDTNRLIPNEADYTLDLVFDAAQLRHTMDRARLLTVMTTNGTVKLTVSGDSVVTTANSPEVGSVHEELTAL
SKEGNDLAISFNPEYLIDALKVIKAPEVRIRFISNVRPFTLQPRNEESGFVQLITPVRTN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=377, Percent_Identity=23.8726790450928, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DPO3B_LACLA (Q9CJJ1)

Other databases:

- EMBL:   AE005176
- PIR:   B86625
- RefSeq:   NP_266158.1
- ProteinModelPortal:   Q9CJJ1
- SMR:   Q9CJJ1
- GeneID:   1113600
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0275
- NMPDR:   fig|272623.1.peg.2
- HOGENOM:   HBG586514
- OMA:   DYNRVIP
- ProtClustDB:   PRK05643
- BioCyc:   LLAC272623:L0275-MONOMER
- BRENDA:   2.7.7.7
- GO:   GO:0005737
- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634
- Gene3D:   G3DSA:3.10.150.10
- SMART:   SM00480
- TIGRFAMs:   TIGR00663

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3

EC number: =2.7.7.7

Molecular weight: Translated: 42265; Mature: 42265

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKFSINKNAFQNALRITKQAIGSKVTIPALTKLKIEVEENGITLIGSNGQISIKNFLPV
CEEEECCCHHHHHHHHHHHHHHCCEEEECEEEEEEEEEECCCEEEEECCCEEEEEEECCC
DNKDASMLISGTGSVLLEAAFFENVVSQLPEVTLEFTEKEQKQVLLTSGKSEITLKGLDS
CCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCEEEEECCCC
EIYPHLQEISEGSSLKMKVKVLKEIFTETVFAVSTQENRPIFTGVHLETLSTGELKAVAT
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHEEEEECCCCCCEEEEEEEEEECCCCEEEEEC
DSHRMSQRLLPLEDSELKFDVILPSKSINSFKNVFTNDEEEIEIFISGSQMLFRNETISY
CCHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCEEEEEEECCEEEEECCHHHH
YSRLIEGSYPDTNRLIPNEADYTLDLVFDAAQLRHTMDRARLLTVMTTNGTVKLTVSGDS
HHHHHCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHEEEEEEEECCCEEEEEECCCE
VVTTANSPEVGSVHEELTALSKEGNDLAISFNPEYLIDALKVIKAPEVRIRFISNVRPFT
EEEECCCCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCEE
LQPRNEESGFVQLITPVRTN
ECCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MIKFSINKNAFQNALRITKQAIGSKVTIPALTKLKIEVEENGITLIGSNGQISIKNFLPV
CEEEECCCHHHHHHHHHHHHHHCCEEEECEEEEEEEEEECCCEEEEECCCEEEEEEECCC
DNKDASMLISGTGSVLLEAAFFENVVSQLPEVTLEFTEKEQKQVLLTSGKSEITLKGLDS
CCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCEEEEECCCC
EIYPHLQEISEGSSLKMKVKVLKEIFTETVFAVSTQENRPIFTGVHLETLSTGELKAVAT
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHEEEEECCCCCCEEEEEEEEEECCCCEEEEEC
DSHRMSQRLLPLEDSELKFDVILPSKSINSFKNVFTNDEEEIEIFISGSQMLFRNETISY
CCHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCEEEEEEECCEEEEECCHHHH
YSRLIEGSYPDTNRLIPNEADYTLDLVFDAAQLRHTMDRARLLTVMTTNGTVKLTVSGDS
HHHHHCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHEEEEEEEECCCEEEEEECCCE
VVTTANSPEVGSVHEELTALSKEGNDLAISFNPEYLIDALKVIKAPEVRIRFISNVRPFT
EEEECCCCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCEE
LQPRNEESGFVQLITPVRTN
ECCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471