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The map label for this gene is gltX

Identifier: 15835345

GI number: 15835345

Start: 867209

End: 868729

Strand: Reverse

Name: gltX

Synonym: TC0730

Alternate gene names: 15835345

Gene position: 868729-867209 (Counterclockwise)

Preceding gene: 15835346

Following gene: 15835343

Centisome position: 80.97

GC content: 40.5

Gene sequence:

>1521_bases
ATGACTTTCCAAAATGTACGCGTTAGAGTGGCCCCTTCTCCTACAGGAGATCCTCATGTGGGGACTGCTTATATGGCTCT
GTTTAATGAGGTTTTTGCAAGAAAATTTAATGGGAAAATGATTTTGAGGATTGAAGATACGGATCAAACTCGAAGTCGTG
ATGATTATGAAAAAAATATTTTCTCGGCGCTCAAGTGGTGTGGAATTCGTTGGGATGAGGGGCCAGATATCGGAGGCCCC
TATGGACCTTATCGTCAATCTGAGCGCACAGAAATCTATAAAAAATATGCAGAAATCCTTTTGCAAACAGATTATGCGTA
TAAATGTTTTGCTACTCCTCAAGAGCTGCAAGAAATGCGAGCAGTGGCTAGTACCTTAGGGTACAGAGGAGGATATGATC
GTCGGTATCGTTACCTTTCCGCAGAAGAGGTTCGACAAAGAGAGGAGCAGGGGCAACCATATACTATTCGCTTGAAAGTG
CCTTTAACTGGGGAGAGTGTTTTTGAAGATCAGTGTAAAGGGCGTGTAGTTTTCCCTTGGGCGGATGTTGATGATCAAGT
TTTGGTAAAATCGGATGGATTCCCTACTTATCATTTTGCCAATGTCGTTGATGATCATTTGATGGGGATCACACATGTGT
TGCGTGGGGAAGAGTGGTTAAGTTCTACCCCTAAACATCTTCTTCTTTATGAAGCTTTTGGTTGGGAACCTCCTCAGTTT
TTCCATATGCCGCTTCTTCTAAACCCTGATGGAAGTAAGTTATCAAAAAGAAAGAATCCTACTTCTATCTTCTATTATCG
AGATGCTGGATATAAGAAAGAGGCGTTTATGAATTTCCTAACCCTAATGGGATATAGTATGGAAGGGGACGAGGAAATTT
ATTCCATGCAACGTCTGATAGAAGCTTTCGATCCTAAACGTATTGGTAGATCCGGAGCTGTTTTTGATATCCGTAAGCTT
GATTGGATGAATAAACATTATCTCAATCATGAGGGATCTCCGGAGAGTCTTTTAAAAGAGTTGAAGGATTGGTTGTGGAA
TGATGAGTTTTTATTGAAAATTCTTCCTCTCTGCCAGACTCGAATCACTACGTTAGCAGATTTCATCCGATTAACTAGTT
TCTTCTTCATGGCAATTCCAGAGTATTCTAAGGAAGATTTATTGCCATCTTCTCTTGGTCATCAGCAAGCCGCGGTAATG
CTTTATAGTTTAGTTAAATATTTGGAGAAGAAAGATTTATGGGAAAAAGATTTCTTCTATCAAGGATCTAAGTGGCTGGC
TGAGGCTTTCCAAGTTCATCACAAAAAGGCTGTAATTCCTTTGTTATATGTTACTATTACAGGGGAAAAACAAGGGTTGC
CTCTTTTTGATTCTATGGAATTATTAGGGAAGGCTCGGACAAGAGCTCGGCTCACTCATGCTCAAAATTTATTGGGAGGA
GTTCCAAAGAAACTGCAGCAACAGATCGATAAGGCATTGCAGGATCAGCCATTAGAAGAAATCCGTTTTTTGGATTTCTA
G

Upstream 100 bases:

>100_bases
GAACGGTCCGATTTTATGCCTGCAAGATAAGACAGGAACCCTGGAGCAGGGAGTTCGTTTCACAAAAATAGAAAAGTTTC
CTTTCCTGGGTTAGTAGCGA

Downstream 100 bases:

>100_bases
TTCTGGGAAAGTTCTTTAAGATTTAGAGAGCTTGGCTGAACTGTTGTGACAAAAGTCTTTTCAAATACAGCCTGAGCTAA
AGAGAAATTTATGGCTTGGG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MTFQNVRVRVAPSPTGDPHVGTAYMALFNEVFARKFNGKMILRIEDTDQTRSRDDYEKNIFSALKWCGIRWDEGPDIGGP
YGPYRQSERTEIYKKYAEILLQTDYAYKCFATPQELQEMRAVASTLGYRGGYDRRYRYLSAEEVRQREEQGQPYTIRLKV
PLTGESVFEDQCKGRVVFPWADVDDQVLVKSDGFPTYHFANVVDDHLMGITHVLRGEEWLSSTPKHLLLYEAFGWEPPQF
FHMPLLLNPDGSKLSKRKNPTSIFYYRDAGYKKEAFMNFLTLMGYSMEGDEEIYSMQRLIEAFDPKRIGRSGAVFDIRKL
DWMNKHYLNHEGSPESLLKELKDWLWNDEFLLKILPLCQTRITTLADFIRLTSFFFMAIPEYSKEDLLPSSLGHQQAAVM
LYSLVKYLEKKDLWEKDFFYQGSKWLAEAFQVHHKKAVIPLLYVTITGEKQGLPLFDSMELLGKARTRARLTHAQNLLGG
VPKKLQQQIDKALQDQPLEEIRFLDF

Sequences:

>Translated_506_residues
MTFQNVRVRVAPSPTGDPHVGTAYMALFNEVFARKFNGKMILRIEDTDQTRSRDDYEKNIFSALKWCGIRWDEGPDIGGP
YGPYRQSERTEIYKKYAEILLQTDYAYKCFATPQELQEMRAVASTLGYRGGYDRRYRYLSAEEVRQREEQGQPYTIRLKV
PLTGESVFEDQCKGRVVFPWADVDDQVLVKSDGFPTYHFANVVDDHLMGITHVLRGEEWLSSTPKHLLLYEAFGWEPPQF
FHMPLLLNPDGSKLSKRKNPTSIFYYRDAGYKKEAFMNFLTLMGYSMEGDEEIYSMQRLIEAFDPKRIGRSGAVFDIRKL
DWMNKHYLNHEGSPESLLKELKDWLWNDEFLLKILPLCQTRITTLADFIRLTSFFFMAIPEYSKEDLLPSSLGHQQAAVM
LYSLVKYLEKKDLWEKDFFYQGSKWLAEAFQVHHKKAVIPLLYVTITGEKQGLPLFDSMELLGKARTRARLTHAQNLLGG
VPKKLQQQIDKALQDQPLEEIRFLDF
>Mature_505_residues
TFQNVRVRVAPSPTGDPHVGTAYMALFNEVFARKFNGKMILRIEDTDQTRSRDDYEKNIFSALKWCGIRWDEGPDIGGPY
GPYRQSERTEIYKKYAEILLQTDYAYKCFATPQELQEMRAVASTLGYRGGYDRRYRYLSAEEVRQREEQGQPYTIRLKVP
LTGESVFEDQCKGRVVFPWADVDDQVLVKSDGFPTYHFANVVDDHLMGITHVLRGEEWLSSTPKHLLLYEAFGWEPPQFF
HMPLLLNPDGSKLSKRKNPTSIFYYRDAGYKKEAFMNFLTLMGYSMEGDEEIYSMQRLIEAFDPKRIGRSGAVFDIRKLD
WMNKHYLNHEGSPESLLKELKDWLWNDEFLLKILPLCQTRITTLADFIRLTSFFFMAIPEYSKEDLLPSSLGHQQAAVML
YSLVKYLEKKDLWEKDFFYQGSKWLAEAFQVHHKKAVIPLLYVTITGEKQGLPLFDSMELLGKARTRARLTHAQNLLGGV
PKKLQQQIDKALQDQPLEEIRFLDF

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=485, Percent_Identity=36.9072164948454, Blast_Score=291, Evalue=7e-79,
Organism=Homo sapiens, GI62241042, Length=258, Percent_Identity=27.1317829457364, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788743, Length=486, Percent_Identity=31.2757201646091, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI226510925, Length=298, Percent_Identity=29.5302013422819, Blast_Score=122, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71993394, Length=487, Percent_Identity=35.7289527720739, Blast_Score=275, Evalue=5e-74,
Organism=Saccharomyces cerevisiae, GI6324540, Length=501, Percent_Identity=36.9261477045908, Blast_Score=286, Evalue=7e-78,
Organism=Drosophila melanogaster, GI24665321, Length=490, Percent_Identity=38.5714285714286, Blast_Score=299, Evalue=2e-81,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_CHLMU (Q9PJU7)

Other databases:

- EMBL:   AE002160
- PIR:   F81671
- RefSeq:   NP_297104.1
- ProteinModelPortal:   Q9PJU7
- SMR:   Q9PJU7
- GeneID:   1246093
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0730
- TIGR:   TC_0730
- HOGENOM:   HBG628189
- OMA:   NEIFAKR
- PhylomeDB:   Q9PJU7
- ProtClustDB:   PRK01406
- BioCyc:   CMUR243161:TC_0730-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 58981; Mature: 58850

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 256-256

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFQNVRVRVAPSPTGDPHVGTAYMALFNEVFARKFNGKMILRIEDTDQTRSRDDYEKNI
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHH
FSALKWCGIRWDEGPDIGGPYGPYRQSERTEIYKKYAEILLQTDYAYKCFATPQELQEMR
HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHH
AVASTLGYRGGYDRRYRYLSAEEVRQREEQGQPYTIRLKVPLTGESVFEDQCKGRVVFPW
HHHHHHCCCCCCHHHHEECCHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCCEEEEEE
ADVDDQVLVKSDGFPTYHFANVVDDHLMGITHVLRGEEWLSSTPKHLLLYEAFGWEPPQF
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEHHCCCCCCCC
FHMPLLLNPDGSKLSKRKNPTSIFYYRDAGYKKEAFMNFLTLMGYSMEGDEEIYSMQRLI
EECCEEECCCCHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EAFDPKRIGRSGAVFDIRKLDWMNKHYLNHEGSPESLLKELKDWLWNDEFLLKILPLCQT
HHHCHHHCCCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
RITTLADFIRLTSFFFMAIPEYSKEDLLPSSLGHQQAAVMLYSLVKYLEKKDLWEKDFFY
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
QGSKWLAEAFQVHHKKAVIPLLYVTITGEKQGLPLFDSMELLGKARTRARLTHAQNLLGG
CCHHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
VPKKLQQQIDKALQDQPLEEIRFLDF
CCHHHHHHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure 
TFQNVRVRVAPSPTGDPHVGTAYMALFNEVFARKFNGKMILRIEDTDQTRSRDDYEKNI
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHH
FSALKWCGIRWDEGPDIGGPYGPYRQSERTEIYKKYAEILLQTDYAYKCFATPQELQEMR
HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHH
AVASTLGYRGGYDRRYRYLSAEEVRQREEQGQPYTIRLKVPLTGESVFEDQCKGRVVFPW
HHHHHHCCCCCCHHHHEECCHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCCEEEEEE
ADVDDQVLVKSDGFPTYHFANVVDDHLMGITHVLRGEEWLSSTPKHLLLYEAFGWEPPQF
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEHHCCCCCCCC
FHMPLLLNPDGSKLSKRKNPTSIFYYRDAGYKKEAFMNFLTLMGYSMEGDEEIYSMQRLI
EECCEEECCCCHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EAFDPKRIGRSGAVFDIRKLDWMNKHYLNHEGSPESLLKELKDWLWNDEFLLKILPLCQT
HHHCHHHCCCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
RITTLADFIRLTSFFFMAIPEYSKEDLLPSSLGHQQAAVMLYSLVKYLEKKDLWEKDFFY
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
QGSKWLAEAFQVHHKKAVIPLLYVTITGEKQGLPLFDSMELLGKARTRARLTHAQNLLGG
CCHHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
VPKKLQQQIDKALQDQPLEEIRFLDF
CCHHHHHHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935