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The map label for this gene is glyA

Identifier: 15835331

GI number: 15835331

Start: 853609

End: 855102

Strand: Reverse

Name: glyA

Synonym: TC0716

Alternate gene names: 15835331

Gene position: 855102-853609 (Counterclockwise)

Preceding gene: 15835334

Following gene: 15835330

Centisome position: 79.7

GC content: 41.37

Gene sequence:

>1494_bases
ATGACTTCTTTATTAGATAAATATTTGAGAAATATTTCCGGGAAAGGACAGCAAAGTTTAGCATCCGTAGCTTATTTAGC
AGCATTAGATCATCTATTACACTCTTTCCCATCCATAGGACAAAGCATAGTACAAGAATTGAAAAGTCAGCGATCTCGTT
TGAAAATGATTGCTTCAGAGAACTTTTCTTCTCTATCTGTTCAGCTTGCTATGGGTAATTTACTCACAGATAAGTATTGT
GAGGGAAGTCCATTCAAGCGTTTTTATTCTTGTTGTGAGAATGTGGACGCTATTGAGTGGGAGTGCGTGGAGACGGCAAA
GGAATTATTTGGTGCAGAAAGTGCTTGTGTTCAGCCACATTCTGGAGCTGATGCAAACTTACTGGCTATTATGTCGATTA
TTACGCAGAAGATCCAAAGCCCAGCTGTTCAGAGACTGGGATACAAGACAATCAACGATCTCCCGGAACAAGAGTATGAG
GCTTTAAAGGCCGAAATGTCTCAATACAAATGCCTAGGCCCGTCTTTAAATTCTGGCGGACATTTAACGCATGGGACTGT
GCGTATAAATGTTATGTCTAAATTGATGCATTGTCTTCCTTATGAGGTCAACTTAGATACCGAGTTATTTGATTATGACG
TGATAGCGAAAATAGCGAAAGAACATAGGCCTACTGTTCTTATTGCGGGCTACTCTTCTTATTCTAGACGATTAAACTTT
GCTACACTGAAGCAAATTGCGGAAGATTGCGGTGCAGTTTTATGGGTGGATATGGCTCATTTCGCAGGATTAGTTGCTGG
AGGTGTTTTTATCGGAGAAGAAAATCCTATTCCTTATGCAGATATCGTTACAACAACTACTCATAAGACTCTGCGAGGGC
CAAGAGGGGGGCTTGTTTTAGCTAAAAAAGAATATTCTGATACTCTAAACAAAGCTTGTCCGTTAATGATGGGAGGTCCG
CTTCCTCATGTAATTGCTGCCAAAGCAGTGGCTTTGAAAGAGGCTATGACAATAAATTTCAGGAAATATGCGCACCAGGT
TGTCGAAAATGCTCGAACTTTAGCTGAAATTTTTCAGCGTAATGGTTTGAGATTATTAACTGGAGGAACCGATAATCATA
TGCTGATTATCGACCTGACTTCTCTTGGAGTTCCTGGACGTATCGCGGAGGATATGTTAACCTCTGTAGGTATTGCGGTG
AATCGGAATTCTATTCCATCGGATGCTTCTGGACAGTGGAAAACTTCGGGTATTCGGTTAGGTACACCTGCTCTAACAAC
ATTAGGTATGGGTAGTGCTGAAATGGAGGAAGTTGCGAATATTATCGCGAAAGTATTGCGAAATATTACTGTGAGACGCA
ATGCTGAGGGCAGTTCTAGTAAAAGTGAGGGAGTGCTATCGGAGGAGATCGCTCAAGAAGCGAGACAACGAGTAGCTGAT
TTATTAGGAAGATTCCCTCTTTACCCTGAAATCGATCTGGAAACGCTAGTTTAG

Upstream 100 bases:

>100_bases
GTTCGTCCTAGTTGAAATGAGACTCCTGAGAAGGATTGTAGCAAATAAGAAAAGGGTTCCTCAGTAAGATTTTGGGGATT
CAATCAGTGGAGGTGGAAAT

Downstream 100 bases:

>100_bases
TGGGAGACTCTATGCCTGAAGGGGAAATGATGCATAAGTTACAAGATATCATAGATAGAAAGTTGTTAGATTCTCGGCGT
ATTTTTTTCTCTGAGCCTGT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MTSLLDKYLRNISGKGQQSLASVAYLAALDHLLHSFPSIGQSIVQELKSQRSRLKMIASENFSSLSVQLAMGNLLTDKYC
EGSPFKRFYSCCENVDAIEWECVETAKELFGAESACVQPHSGADANLLAIMSIITQKIQSPAVQRLGYKTINDLPEQEYE
ALKAEMSQYKCLGPSLNSGGHLTHGTVRINVMSKLMHCLPYEVNLDTELFDYDVIAKIAKEHRPTVLIAGYSSYSRRLNF
ATLKQIAEDCGAVLWVDMAHFAGLVAGGVFIGEENPIPYADIVTTTTHKTLRGPRGGLVLAKKEYSDTLNKACPLMMGGP
LPHVIAAKAVALKEAMTINFRKYAHQVVENARTLAEIFQRNGLRLLTGGTDNHMLIIDLTSLGVPGRIAEDMLTSVGIAV
NRNSIPSDASGQWKTSGIRLGTPALTTLGMGSAEMEEVANIIAKVLRNITVRRNAEGSSSKSEGVLSEEIAQEARQRVAD
LLGRFPLYPEIDLETLV

Sequences:

>Translated_497_residues
MTSLLDKYLRNISGKGQQSLASVAYLAALDHLLHSFPSIGQSIVQELKSQRSRLKMIASENFSSLSVQLAMGNLLTDKYC
EGSPFKRFYSCCENVDAIEWECVETAKELFGAESACVQPHSGADANLLAIMSIITQKIQSPAVQRLGYKTINDLPEQEYE
ALKAEMSQYKCLGPSLNSGGHLTHGTVRINVMSKLMHCLPYEVNLDTELFDYDVIAKIAKEHRPTVLIAGYSSYSRRLNF
ATLKQIAEDCGAVLWVDMAHFAGLVAGGVFIGEENPIPYADIVTTTTHKTLRGPRGGLVLAKKEYSDTLNKACPLMMGGP
LPHVIAAKAVALKEAMTINFRKYAHQVVENARTLAEIFQRNGLRLLTGGTDNHMLIIDLTSLGVPGRIAEDMLTSVGIAV
NRNSIPSDASGQWKTSGIRLGTPALTTLGMGSAEMEEVANIIAKVLRNITVRRNAEGSSSKSEGVLSEEIAQEARQRVAD
LLGRFPLYPEIDLETLV
>Mature_496_residues
TSLLDKYLRNISGKGQQSLASVAYLAALDHLLHSFPSIGQSIVQELKSQRSRLKMIASENFSSLSVQLAMGNLLTDKYCE
GSPFKRFYSCCENVDAIEWECVETAKELFGAESACVQPHSGADANLLAIMSIITQKIQSPAVQRLGYKTINDLPEQEYEA
LKAEMSQYKCLGPSLNSGGHLTHGTVRINVMSKLMHCLPYEVNLDTELFDYDVIAKIAKEHRPTVLIAGYSSYSRRLNFA
TLKQIAEDCGAVLWVDMAHFAGLVAGGVFIGEENPIPYADIVTTTTHKTLRGPRGGLVLAKKEYSDTLNKACPLMMGGPL
PHVIAAKAVALKEAMTINFRKYAHQVVENARTLAEIFQRNGLRLLTGGTDNHMLIIDLTSLGVPGRIAEDMLTSVGIAVN
RNSIPSDASGQWKTSGIRLGTPALTTLGMGSAEMEEVANIIAKVLRNITVRRNAEGSSSKSEGVLSEEIAQEARQRVADL
LGRFPLYPEIDLETLV

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=493, Percent_Identity=36.7139959432049, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI261862350, Length=493, Percent_Identity=36.7139959432049, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI261862348, Length=493, Percent_Identity=36.7139959432049, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI19923315, Length=493, Percent_Identity=36.7139959432049, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI22547186, Length=476, Percent_Identity=36.1344537815126, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI261862346, Length=493, Percent_Identity=36.105476673428, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI22547189, Length=460, Percent_Identity=35.4347826086957, Blast_Score=239, Evalue=3e-63,
Organism=Escherichia coli, GI1788902, Length=452, Percent_Identity=41.3716814159292, Blast_Score=315, Evalue=5e-87,
Organism=Caenorhabditis elegans, GI25144729, Length=479, Percent_Identity=36.7432150313152, Blast_Score=290, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI25144732, Length=479, Percent_Identity=36.7432150313152, Blast_Score=289, Evalue=3e-78,
Organism=Saccharomyces cerevisiae, GI6319739, Length=435, Percent_Identity=37.2413793103448, Blast_Score=276, Evalue=8e-75,
Organism=Saccharomyces cerevisiae, GI6323087, Length=440, Percent_Identity=37.0454545454545, Blast_Score=270, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24640005, Length=520, Percent_Identity=36.7307692307692, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI221329721, Length=491, Percent_Identity=37.4745417515275, Blast_Score=289, Evalue=3e-78,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_CHLMU (Q9PJW0)

Other databases:

- EMBL:   AE002160
- PIR:   C81672
- RefSeq:   NP_297090.1
- ProteinModelPortal:   Q9PJW0
- GeneID:   1246079
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0716
- TIGR:   TC_0716
- HOGENOM:   HBG301263
- OMA:   LNGNRMF
- ProtClustDB:   PRK13580
- BioCyc:   CMUR243161:TC_0716-MONOMER
- BRENDA:   2.1.2.1
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 54233; Mature: 54102

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLLDKYLRNISGKGQQSLASVAYLAALDHLLHSFPSIGQSIVQELKSQRSRLKMIASE
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NFSSLSVQLAMGNLLTDKYCEGSPFKRFYSCCENVDAIEWECVETAKELFGAESACVQPH
CCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHCCCCC
SGADANLLAIMSIITQKIQSPAVQRLGYKTINDLPEQEYEALKAEMSQYKCLGPSLNSGG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHEECCCCCCCCC
HLTHGTVRINVMSKLMHCLPYEVNLDTELFDYDVIAKIAKEHRPTVLIAGYSSYSRRLNF
CEEECEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHHHHCHH
ATLKQIAEDCGAVLWVDMAHFAGLVAGGVFIGEENPIPYADIVTTTTHKTLRGPRGGLVL
HHHHHHHHHCCHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHEECCCHHHHCCCCCCEEE
AKKEYSDTLNKACPLMMGGPLPHVIAAKAVALKEAMTINFRKYAHQVVENARTLAEIFQR
EHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NGLRLLTGGTDNHMLIIDLTSLGVPGRIAEDMLTSVGIAVNRNSIPSDASGQWKTSGIRL
CCCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHCCEEECCCCCCCCCCCEECCCEEE
GTPALTTLGMGSAEMEEVANIIAKVLRNITVRRNAEGSSSKSEGVLSEEIAQEARQRVAD
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LLGRFPLYPEIDLETLV
HHHCCCCCCCCCHHHCC
>Mature Secondary Structure 
TSLLDKYLRNISGKGQQSLASVAYLAALDHLLHSFPSIGQSIVQELKSQRSRLKMIASE
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NFSSLSVQLAMGNLLTDKYCEGSPFKRFYSCCENVDAIEWECVETAKELFGAESACVQPH
CCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHCCCCC
SGADANLLAIMSIITQKIQSPAVQRLGYKTINDLPEQEYEALKAEMSQYKCLGPSLNSGG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHEECCCCCCCCC
HLTHGTVRINVMSKLMHCLPYEVNLDTELFDYDVIAKIAKEHRPTVLIAGYSSYSRRLNF
CEEECEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHHHHCHH
ATLKQIAEDCGAVLWVDMAHFAGLVAGGVFIGEENPIPYADIVTTTTHKTLRGPRGGLVL
HHHHHHHHHCCHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHEECCCHHHHCCCCCCEEE
AKKEYSDTLNKACPLMMGGPLPHVIAAKAVALKEAMTINFRKYAHQVVENARTLAEIFQR
EHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NGLRLLTGGTDNHMLIIDLTSLGVPGRIAEDMLTSVGIAVNRNSIPSDASGQWKTSGIRL
CCCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHCCEEECCCCCCCCCCCEECCCEEE
GTPALTTLGMGSAEMEEVANIIAKVLRNITVRRNAEGSSSKSEGVLSEEIAQEARQRVAD
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LLGRFPLYPEIDLETLV
HHHCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935