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Identifier: 15835089

GI number: 15835089

Start: 571046

End: 572608

Strand: Direct

Name: Not Available

Synonym: TC0471

Alternate gene names: 15835089

Gene position: 571046-572608 (Clockwise)

Preceding gene: 15835088

Following gene: 15835090

Centisome position: 53.22

GC content: 40.05

Gene sequence:

>1563_bases
ATGCGCAAGGTATCAGTGGGAATCTGCTTGCTTATCGCATTAGCAACAGCAATTACCGGCTGCTCCAAATCCTCCTCAAA
CAAATCAAATCACTCCTCCTCTAATCAAAGCGTATCTGTGAGCATGAAGGACGATCCTCGCACGTTTGACCCTCGAGAGG
TTAGGCTTCTTTCGGATATTAATTTGATTCACCATCTTTATGAGGGACTGGTTCAAGAGACTCCTTCCGGAGAGGTTTTC
CCTGCATTAGCAGAAAGTTTCTTCCTATCTGAAGATAAGAAAACCTACACTTTCCACCTAAAAAAAGCACTCTGGAGTAA
TGGAGACCTGATTACTGCTCATGATTTTGTACGTTCTTGGAACGATGTTTTGCAAAATCGCATTGCAAGTATCTACTCTT
TCGCTTTTTTACCCATTGATTTAAGTAAAGATTGTGGATTCTTCGCTAAGGACAATCATACACTTGTTATCAATTTGCAT
ACCCCTACTCCACACTTTCTGAAATTACTTACCCTTCCAGTATTTTATCCTGTGCACCCCGAACATCAAATTCGGAATGA
AGCAAAAGCTCTTCCAATATCTACAGGGGCTTTTTGTTTAAAAGAGAAAAAAGATCGGCGGTGGCTTAAATTAGAAAAAA
ATCCCTATTATTATAACAAAGAGCAAGTTGCGATCCAGGAAATCCACATACATGTGATTCCTGATCAGCAGACCGCTTCT
GCTTTGTTCAAACAAGGAAAATTGGATTGGCAAGGACTTCCTTGGGGACACAGCATTCCCCAAGAAGCTTTAGCAACAGC
AAATAAACGTCGTACTCCTCAATCATTTGATATATCTGGCACCTCGTGGCTCACGTTCAACACATCAAAAGTACCCTTCA
GCCATCCCAAGTTGCGACAAGCTTTAAGCCTTGTTCTAAATAAGGAAGCCTTAGCCTCTCCAACCTTTGTTAAGCCCGCT
AAACACCTACTTCCTACACACCTGCACACTTATCCAGAGCAACCTACTTACAAACAACAAGAAGCCGTCATTTTAGCCAA
AACTCTGTTACAAGAAGCATTAACAGATCTTAATATGACTATAAAAGATCTTGAAAAATGCCCTTTGATTTTTTCAGCGA
CATCTAGCGTCAACTCACAAATGGCCCAAATGATGCGCGATCAATGGCGAAGAATTTTAGGAATAACATTCCCTATTTGC
GGGAAAGAGTATGCTTTATTACAAAATGATTTGACCACCAACACTTTTTTTATGTCCATAAACGGTTGGTTTGCAGACTT
TTCCGATCCTTTAGCCTTCTTGTCTGTTTTTTCTTCTAAGGGAATCAAACCTTATGCCTTACAAGACCCTCTGTTTGACC
AGCTTATTCTCTCTATAGAAACGGAAAAAGATCCTCGAAAGCGTATTTCTCTGATTTCCGAAGCTTCTCTATACCTAGAA
AAACAAAATATCATAGAACCGCTCTATCACGATGTTTTTCACTATGCCGCAAATAATAAACTTTCTTTTGTTAGATTACA
TCCCTCAGGTCTTGTCGATATGCGGTATGCAAAAAACTCTTGA

Upstream 100 bases:

>100_bases
TCCCTTCTTCGCAGTTGTGCTCTGATAATGCTGCAATGATAGCTGGATTAGGAGAGAGACTGTTTTGCAATCAGACATAT
GTTTCTAAGGAGGTTATTCC

Downstream 100 bases:

>100_bases
AGGTATTTTACTCCGTGCGCAGAAAAATCCCTCCCTTTAAAATAACGCGGAAGAAAGCACTCTGTATTTTAAAGTAACTC
TGTGATCAAACATAACGTGC

Product: peptide ABC transporter, periplasmic peptide-binding protein, putative

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MRKVSVGICLLIALATAITGCSKSSSNKSNHSSSNQSVSVSMKDDPRTFDPREVRLLSDINLIHHLYEGLVQETPSGEVF
PALAESFFLSEDKKTYTFHLKKALWSNGDLITAHDFVRSWNDVLQNRIASIYSFAFLPIDLSKDCGFFAKDNHTLVINLH
TPTPHFLKLLTLPVFYPVHPEHQIRNEAKALPISTGAFCLKEKKDRRWLKLEKNPYYYNKEQVAIQEIHIHVIPDQQTAS
ALFKQGKLDWQGLPWGHSIPQEALATANKRRTPQSFDISGTSWLTFNTSKVPFSHPKLRQALSLVLNKEALASPTFVKPA
KHLLPTHLHTYPEQPTYKQQEAVILAKTLLQEALTDLNMTIKDLEKCPLIFSATSSVNSQMAQMMRDQWRRILGITFPIC
GKEYALLQNDLTTNTFFMSINGWFADFSDPLAFLSVFSSKGIKPYALQDPLFDQLILSIETEKDPRKRISLISEASLYLE
KQNIIEPLYHDVFHYAANNKLSFVRLHPSGLVDMRYAKNS

Sequences:

>Translated_520_residues
MRKVSVGICLLIALATAITGCSKSSSNKSNHSSSNQSVSVSMKDDPRTFDPREVRLLSDINLIHHLYEGLVQETPSGEVF
PALAESFFLSEDKKTYTFHLKKALWSNGDLITAHDFVRSWNDVLQNRIASIYSFAFLPIDLSKDCGFFAKDNHTLVINLH
TPTPHFLKLLTLPVFYPVHPEHQIRNEAKALPISTGAFCLKEKKDRRWLKLEKNPYYYNKEQVAIQEIHIHVIPDQQTAS
ALFKQGKLDWQGLPWGHSIPQEALATANKRRTPQSFDISGTSWLTFNTSKVPFSHPKLRQALSLVLNKEALASPTFVKPA
KHLLPTHLHTYPEQPTYKQQEAVILAKTLLQEALTDLNMTIKDLEKCPLIFSATSSVNSQMAQMMRDQWRRILGITFPIC
GKEYALLQNDLTTNTFFMSINGWFADFSDPLAFLSVFSSKGIKPYALQDPLFDQLILSIETEKDPRKRISLISEASLYLE
KQNIIEPLYHDVFHYAANNKLSFVRLHPSGLVDMRYAKNS
>Mature_520_residues
MRKVSVGICLLIALATAITGCSKSSSNKSNHSSSNQSVSVSMKDDPRTFDPREVRLLSDINLIHHLYEGLVQETPSGEVF
PALAESFFLSEDKKTYTFHLKKALWSNGDLITAHDFVRSWNDVLQNRIASIYSFAFLPIDLSKDCGFFAKDNHTLVINLH
TPTPHFLKLLTLPVFYPVHPEHQIRNEAKALPISTGAFCLKEKKDRRWLKLEKNPYYYNKEQVAIQEIHIHVIPDQQTAS
ALFKQGKLDWQGLPWGHSIPQEALATANKRRTPQSFDISGTSWLTFNTSKVPFSHPKLRQALSLVLNKEALASPTFVKPA
KHLLPTHLHTYPEQPTYKQQEAVILAKTLLQEALTDLNMTIKDLEKCPLIFSATSSVNSQMAQMMRDQWRRILGITFPIC
GKEYALLQNDLTTNTFFMSINGWFADFSDPLAFLSVFSSKGIKPYALQDPLFDQLILSIETEKDPRKRISLISEASLYLE
KQNIIEPLYHDVFHYAANNKLSFVRLHPSGLVDMRYAKNS

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=481, Percent_Identity=27.4428274428274, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI87081878, Length=513, Percent_Identity=25.9259259259259, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789397, Length=474, Percent_Identity=24.2616033755274, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1789887, Length=458, Percent_Identity=27.5109170305677, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787762, Length=418, Percent_Identity=24.4019138755981, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59117; Mature: 59117

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00126 PDEASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVSVGICLLIALATAITGCSKSSSNKSNHSSSNQSVSVSMKDDPRTFDPREVRLLSDI
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
NLIHHLYEGLVQETPSGEVFPALAESFFLSEDKKTYTFHLKKALWSNGDLITAHDFVRSW
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCEEEHHHHHHHH
NDVLQNRIASIYSFAFLPIDLSKDCGFFAKDNHTLVINLHTPTPHFLKLLTLPVFYPVHP
HHHHHHHHHHHHHHHEEEEECCCCCCCEECCCCEEEEEEECCCHHHHHHHHHHHEECCCC
EHQIRNEAKALPISTGAFCLKEKKDRRWLKLEKNPYYYNKEQVAIQEIHIHVIPDQQTAS
HHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEECCCCEECCCCEEEEEEEEEEECCCHHHH
ALFKQGKLDWQGLPWGHSIPQEALATANKRRTPQSFDISGTSWLTFNTSKVPFSHPKLRQ
HHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHH
ALSLVLNKEALASPTFVKPAKHLLPTHLHTYPEQPTYKQQEAVILAKTLLQEALTDLNMT
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
IKDLEKCPLIFSATSSVNSQMAQMMRDQWRRILGITFPICGKEYALLQNDLTTNTFFMSI
HHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEEEE
NGWFADFSDPLAFLSVFSSKGIKPYALQDPLFDQLILSIETEKDPRKRISLISEASLYLE
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHH
KQNIIEPLYHDVFHYAANNKLSFVRLHPSGLVDMRYAKNS
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCC
>Mature Secondary Structure
MRKVSVGICLLIALATAITGCSKSSSNKSNHSSSNQSVSVSMKDDPRTFDPREVRLLSDI
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
NLIHHLYEGLVQETPSGEVFPALAESFFLSEDKKTYTFHLKKALWSNGDLITAHDFVRSW
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCEEEHHHHHHHH
NDVLQNRIASIYSFAFLPIDLSKDCGFFAKDNHTLVINLHTPTPHFLKLLTLPVFYPVHP
HHHHHHHHHHHHHHHEEEEECCCCCCCEECCCCEEEEEEECCCHHHHHHHHHHHEECCCC
EHQIRNEAKALPISTGAFCLKEKKDRRWLKLEKNPYYYNKEQVAIQEIHIHVIPDQQTAS
HHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEECCCCEECCCCEEEEEEEEEEECCCHHHH
ALFKQGKLDWQGLPWGHSIPQEALATANKRRTPQSFDISGTSWLTFNTSKVPFSHPKLRQ
HHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHH
ALSLVLNKEALASPTFVKPAKHLLPTHLHTYPEQPTYKQQEAVILAKTLLQEALTDLNMT
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
IKDLEKCPLIFSATSSVNSQMAQMMRDQWRRILGITFPICGKEYALLQNDLTTNTFFMSI
HHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEEEE
NGWFADFSDPLAFLSVFSSKGIKPYALQDPLFDQLILSIETEKDPRKRISLISEASLYLE
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHH
KQNIIEPLYHDVFHYAANNKLSFVRLHPSGLVDMRYAKNS
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]