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The map label for this gene is pckA

Identifier: 15834708

GI number: 15834708

Start: 99211

End: 101010

Strand: Direct

Name: pckA

Synonym: TC0083

Alternate gene names: 15834708

Gene position: 99211-101010 (Clockwise)

Preceding gene: 15834707

Following gene: 15834709

Centisome position: 9.25

GC content: 45.11

Gene sequence:

>1800_bases
ATGACCGGCGATTGGATGTCTAAGATAACCCATTCAGGATTAAAATCCTGGATAGAGGAGGTGATAGCTTTAGTGTCGCC
TAAGGACGTGAGGTTATGCGATGGTTCGGAAGCCGAATACCAACAACTTTGCCAGCAGATGCAAGAAGCTGGGGTAATGA
CCCTATTGAATCCTGAGTTACATCCTAACTGTTTCTTAGTTCGCTCTTCTCCTGATGATGTTGCTCGTGTTGAACAATTT
ACTTTTATTTGTACTAAGACAAAGGAAGAAGCTGGTCCTACGAATAACTGGCGAGATCCTCAAGAGATGCGTGCGGAGCT
ACATGAACTGTTTCAAGGGTGCATGCAAGGGCGTACTCTCTATATCGTTCCATTTTGTATGGGACCTTTACATTCTCCTT
TTTCTTTAGTTGGGATAGAAATAACAGATTCTCCTTATGTTGTGTGTTCTATGAAGATCATGACACGGATGGGGGCTTCT
GTTTTAGAGATGTTGGGATCATCCGGGACCTTTTATAAGTGTTTGCATAGCGTTGGAAAACCCCTAGCTCCCGGAGAAAA
AGATGTAGCATGGCCTTGTAATCCTGGTCACATGCGCATCGTCCATTTTCAAGATGATAGTAGCGTGATGTCTTTTGGTA
GCGGATACGGGGGTAATGCGCTACTTGGTAAGAAATGCGTAGCTTTGCGCTTGGCCTCCTATTTGGGACATAAGCAAGGT
TGGTTAGCTGAGCACATGCTGGTTATTGGAGTGACTAACCCTGAGGGAAGAAAGAAATATTTTGCAGCGGCTTTCCCGAG
TGCTTGTGGGAAGACGAATCTTGCTATGCTGATGCCAAAACTTCCGGGATGGAAGGTAGAGTGTATTGGAGATGATATAG
CATGGATTCGTCCTGGGGATGATGGAAGATTATATGCAGTGAATCCAGAGTTTGGTTTTTTTGGGGTAGCTATAGGCACT
TCAGAGAAAACAAATCCCAATGCATTGGCTACGTGCCACTCTGATTCGATATTTACGAATGTTGCTTTAACATCGGATGG
AGACGTGTGGTGGGAAGGAAAGACTGCTACACCTCCGCAAGGGATGATTGACTGGAAAGGAAGGAACTGGACTCCTGGGG
GAGAACCTGCTGCTCATCCTAATGCGCGTTTTACAGCTCCTTTGGATCACTGTCCTTCTTTAGATCCGCAGTGGGATAGT
CCTCAAGGGGTTCCGCTAGAGGCTATTATTTTTGGAGGGAGACGCACAGAAACGATTCCTTTGGTTTATGAATCATTAAG
TTGGGAGCATGGGGTTATGATGGGGGCAGGGATGTCCTCAACGACTACTGCTGCGATTGCTGGGGAATTGGGAAAACTGC
GGCATGATCCTTTTGCCATGCTTCCGTTTTGTGGATACAATATGGCGGCTTATTTTGAGCATTGGTTGTCTTTTGCTGGG
AAAGGGTTACAGTTGCCTAGGATTTTCAGTGTGAATTGGTTCCGTAAGGATGAGAATGGCCAATTTATTTGGCCGGGATT
TTCTGAAAATCTTCGTGTATTAGAGTGGATTTTCCGTCGCACGGATGGAGAGGACTCTATTGCGCGCCGTACACCAATAG
GATATCTTCCTACTGAAGAGGGACTTAATACGACAGGGCTTAATTTATCTCGAGATGCTTTGCAGTCTTTGCTTGCGGTG
GACACGCAAGGCTGGCGAGCAGAGGTAAATAACATTCGGGAGTATTGTTCTATTTTTGGATCGGATATGCCACGGCAAAT
TCTTGAAGAATTATCTAGAATAGAAAATGAATTGAAATAA

Upstream 100 bases:

>100_bases
CTGTTATTACTGCTCCGCATCCGCTTCTAGCGGTTTGCTTAGGCACAGGGAAAGCTTTGGAACATCTAGATCAATTGAAG
AAACGTAAAGAGAGTTTGGT

Downstream 100 bases:

>100_bases
TTAAAACGAAACGAGTTTTAATTATTTCAATTTAGAAATTATTGTTTTTCTGTTTTAAAAAACAATTTTTATTTTTAAAA
TTAGAAAAATAATAGTTTCT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MTGDWMSKITHSGLKSWIEEVIALVSPKDVRLCDGSEAEYQQLCQQMQEAGVMTLLNPELHPNCFLVRSSPDDVARVEQF
TFICTKTKEEAGPTNNWRDPQEMRAELHELFQGCMQGRTLYIVPFCMGPLHSPFSLVGIEITDSPYVVCSMKIMTRMGAS
VLEMLGSSGTFYKCLHSVGKPLAPGEKDVAWPCNPGHMRIVHFQDDSSVMSFGSGYGGNALLGKKCVALRLASYLGHKQG
WLAEHMLVIGVTNPEGRKKYFAAAFPSACGKTNLAMLMPKLPGWKVECIGDDIAWIRPGDDGRLYAVNPEFGFFGVAIGT
SEKTNPNALATCHSDSIFTNVALTSDGDVWWEGKTATPPQGMIDWKGRNWTPGGEPAAHPNARFTAPLDHCPSLDPQWDS
PQGVPLEAIIFGGRRTETIPLVYESLSWEHGVMMGAGMSSTTTAAIAGELGKLRHDPFAMLPFCGYNMAAYFEHWLSFAG
KGLQLPRIFSVNWFRKDENGQFIWPGFSENLRVLEWIFRRTDGEDSIARRTPIGYLPTEEGLNTTGLNLSRDALQSLLAV
DTQGWRAEVNNIREYCSIFGSDMPRQILEELSRIENELK

Sequences:

>Translated_599_residues
MTGDWMSKITHSGLKSWIEEVIALVSPKDVRLCDGSEAEYQQLCQQMQEAGVMTLLNPELHPNCFLVRSSPDDVARVEQF
TFICTKTKEEAGPTNNWRDPQEMRAELHELFQGCMQGRTLYIVPFCMGPLHSPFSLVGIEITDSPYVVCSMKIMTRMGAS
VLEMLGSSGTFYKCLHSVGKPLAPGEKDVAWPCNPGHMRIVHFQDDSSVMSFGSGYGGNALLGKKCVALRLASYLGHKQG
WLAEHMLVIGVTNPEGRKKYFAAAFPSACGKTNLAMLMPKLPGWKVECIGDDIAWIRPGDDGRLYAVNPEFGFFGVAIGT
SEKTNPNALATCHSDSIFTNVALTSDGDVWWEGKTATPPQGMIDWKGRNWTPGGEPAAHPNARFTAPLDHCPSLDPQWDS
PQGVPLEAIIFGGRRTETIPLVYESLSWEHGVMMGAGMSSTTTAAIAGELGKLRHDPFAMLPFCGYNMAAYFEHWLSFAG
KGLQLPRIFSVNWFRKDENGQFIWPGFSENLRVLEWIFRRTDGEDSIARRTPIGYLPTEEGLNTTGLNLSRDALQSLLAV
DTQGWRAEVNNIREYCSIFGSDMPRQILEELSRIENELK
>Mature_598_residues
TGDWMSKITHSGLKSWIEEVIALVSPKDVRLCDGSEAEYQQLCQQMQEAGVMTLLNPELHPNCFLVRSSPDDVARVEQFT
FICTKTKEEAGPTNNWRDPQEMRAELHELFQGCMQGRTLYIVPFCMGPLHSPFSLVGIEITDSPYVVCSMKIMTRMGASV
LEMLGSSGTFYKCLHSVGKPLAPGEKDVAWPCNPGHMRIVHFQDDSSVMSFGSGYGGNALLGKKCVALRLASYLGHKQGW
LAEHMLVIGVTNPEGRKKYFAAAFPSACGKTNLAMLMPKLPGWKVECIGDDIAWIRPGDDGRLYAVNPEFGFFGVAIGTS
EKTNPNALATCHSDSIFTNVALTSDGDVWWEGKTATPPQGMIDWKGRNWTPGGEPAAHPNARFTAPLDHCPSLDPQWDSP
QGVPLEAIIFGGRRTETIPLVYESLSWEHGVMMGAGMSSTTTAAIAGELGKLRHDPFAMLPFCGYNMAAYFEHWLSFAGK
GLQLPRIFSVNWFRKDENGQFIWPGFSENLRVLEWIFRRTDGEDSIARRTPIGYLPTEEGLNTTGLNLSRDALQSLLAVD
TQGWRAEVNNIREYCSIFGSDMPRQILEELSRIENELK

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family

Homologues:

Organism=Homo sapiens, GI66346721, Length=602, Percent_Identity=52.9900332225914, Blast_Score=634, Evalue=0.0,
Organism=Homo sapiens, GI187281517, Length=601, Percent_Identity=51.414309484193, Blast_Score=614, Evalue=1e-176,
Organism=Homo sapiens, GI66346723, Length=384, Percent_Identity=52.8645833333333, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17555492, Length=610, Percent_Identity=47.8688524590164, Blast_Score=578, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI71989645, Length=604, Percent_Identity=47.682119205298, Blast_Score=576, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI71989658, Length=602, Percent_Identity=47.8405315614618, Blast_Score=575, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI71989653, Length=591, Percent_Identity=48.2233502538071, Blast_Score=571, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI32564411, Length=539, Percent_Identity=50.278293135436, Blast_Score=546, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI32564407, Length=519, Percent_Identity=50.8670520231214, Blast_Score=533, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI71989649, Length=482, Percent_Identity=50.6224066390041, Blast_Score=486, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI32564409, Length=365, Percent_Identity=50.4109589041096, Blast_Score=371, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17540956, Length=630, Percent_Identity=23.3333333333333, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24655085, Length=602, Percent_Identity=50.3322259136213, Blast_Score=603, Evalue=1e-173,
Organism=Drosophila melanogaster, GI24655082, Length=603, Percent_Identity=49.7512437810945, Blast_Score=592, Evalue=1e-169,
Organism=Drosophila melanogaster, GI161077226, Length=556, Percent_Identity=51.2589928057554, Blast_Score=566, Evalue=1e-161,
Organism=Drosophila melanogaster, GI24655088, Length=502, Percent_Identity=51.792828685259, Blast_Score=529, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCKG_CHLMU (Q9PLL6)

Other databases:

- EMBL:   AE002160
- RefSeq:   NP_296467.1
- ProteinModelPortal:   Q9PLL6
- SMR:   Q9PLL6
- GeneID:   1245613
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0083
- TIGR:   TC_0083
- HOGENOM:   HBG484437
- OMA:   CGYHMGD
- ProtClustDB:   PRK04210
- BioCyc:   CMUR243161:TC_0083-MONOMER
- BRENDA:   4.1.1.32
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00452
- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PANTHER:   PTHR11561
- PIRSF:   PIRSF001348

Pfam domain/function: PF00821 PEPCK; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.32

Molecular weight: Translated: 66439; Mature: 66307

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00505 PEPCK_GTP; PS00012 PHOSPHOPANTETHEINE

Important sites: ACT_SITE 269-269 BINDING 76-76 BINDING 218-218 BINDING 225-225 BINDING 267-267 BINDING 383-383 BINDING 414-414

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGDWMSKITHSGLKSWIEEVIALVSPKDVRLCDGSEAEYQQLCQQMQEAGVMTLLNPEL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCEEEEECCCC
HPNCFLVRSSPDDVARVEQFTFICTKTKEEAGPTNNWRDPQEMRAELHELFQGCMQGRTL
CCCEEEEECCCHHHHHHHHEEEEEECCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEE
YIVPFCMGPLHSPFSLVGIEITDSPYVVCSMKIMTRMGASVLEMLGSSGTFYKCLHSVGK
EEEEEECCCCCCCCEEEEEEECCCCEEEEHHHHHHHCCHHHHHHHCCCCHHHHHHHHHCC
PLAPGEKDVAWPCNPGHMRIVHFQDDSSVMSFGSGYGGNALLGKKCVALRLASYLGHKQG
CCCCCCCCCCCCCCCCCEEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
WLAEHMLVIGVTNPEGRKKYFAAAFPSACGKTNLAMLMPKLPGWKVECIGDDIAWIRPGD
CCCCCEEEEEECCCCCCHHEEEECCCHHCCCCCCEEEECCCCCCEEEEECCCEEEEECCC
DGRLYAVNPEFGFFGVAIGTSEKTNPNALATCHSDSIFTNVALTSDGDVWWEGKTATPPQ
CCEEEEECCCCCEEEEEECCCCCCCCCEEEEECCCCEEEEEEEECCCCEEECCCCCCCCC
GMIDWKGRNWTPGGEPAAHPNARFTAPLDHCPSLDPQWDSPQGVPLEAIIFGGRRTETIP
CCEEECCCCCCCCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCCCEEEEEECCCCCCEEH
LVYESLSWEHGVMMGAGMSSTTTAAIAGELGKLRHDPFAMLPFCGYNMAAYFEHWLSFAG
HHEECCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHCC
KGLQLPRIFSVNWFRKDENGQFIWPGFSENLRVLEWIFRRTDGEDSIARRTPIGYLPTEE
CCCCCCEEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCC
GLNTTGLNLSRDALQSLLAVDTQGWRAEVNNIREYCSIFGSDMPRQILEELSRIENELK
CCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGDWMSKITHSGLKSWIEEVIALVSPKDVRLCDGSEAEYQQLCQQMQEAGVMTLLNPEL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCEEEEECCCC
HPNCFLVRSSPDDVARVEQFTFICTKTKEEAGPTNNWRDPQEMRAELHELFQGCMQGRTL
CCCEEEEECCCHHHHHHHHEEEEEECCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEE
YIVPFCMGPLHSPFSLVGIEITDSPYVVCSMKIMTRMGASVLEMLGSSGTFYKCLHSVGK
EEEEEECCCCCCCCEEEEEEECCCCEEEEHHHHHHHCCHHHHHHHCCCCHHHHHHHHHCC
PLAPGEKDVAWPCNPGHMRIVHFQDDSSVMSFGSGYGGNALLGKKCVALRLASYLGHKQG
CCCCCCCCCCCCCCCCCEEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
WLAEHMLVIGVTNPEGRKKYFAAAFPSACGKTNLAMLMPKLPGWKVECIGDDIAWIRPGD
CCCCCEEEEEECCCCCCHHEEEECCCHHCCCCCCEEEECCCCCCEEEEECCCEEEEECCC
DGRLYAVNPEFGFFGVAIGTSEKTNPNALATCHSDSIFTNVALTSDGDVWWEGKTATPPQ
CCEEEEECCCCCEEEEEECCCCCCCCCEEEEECCCCEEEEEEEECCCCEEECCCCCCCCC
GMIDWKGRNWTPGGEPAAHPNARFTAPLDHCPSLDPQWDSPQGVPLEAIIFGGRRTETIP
CCEEECCCCCCCCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCCCEEEEEECCCCCCEEH
LVYESLSWEHGVMMGAGMSSTTTAAIAGELGKLRHDPFAMLPFCGYNMAAYFEHWLSFAG
HHEECCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHCC
KGLQLPRIFSVNWFRKDENGQFIWPGFSENLRVLEWIFRRTDGEDSIARRTPIGYLPTEE
CCCCCCEEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCC
GLNTTGLNLSRDALQSLLAVDTQGWRAEVNNIREYCSIFGSDMPRQILEELSRIENELK
CCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935