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The map label for this gene is tsf

Identifier: 15834675

GI number: 15834675

Start: 56580

End: 57428

Strand: Reverse

Name: tsf

Synonym: TC0050

Alternate gene names: 15834675

Gene position: 57428-56580 (Counterclockwise)

Preceding gene: 15834676

Following gene: 15834674

Centisome position: 5.35

GC content: 41.81

Gene sequence:

>849_bases
ATGAGCGACTTCTCCATGGAAACATTAAAGAGTTTGAGACAGCGAACAGGTGTAGGCCTGACAAAATGTAAAGAAGCCCT
TGAACATGCAAAGGGCAACTTAGAAGATGCTGTTGTTTACTTACGAAAACTTGGACTTGCCTCTGCTGGTAAGAAAGAGC
ACCGAGAAACGAAAGAAGGTGTCATTGCTGCGTCGGTTGATGAGCATGGGGCAGCTATCGTCGAAGTGAATGTTGAGACG
GACTTTGTTGCTAACAATAGCGTTTTCCGAACGTTTGTTACAGGTTTATTATCCGACATCCTCAACAACAAACTGAGTGA
TGTGGATGCTTTAGCTCAAGTAACGTCCTCTCAAGAGCCTTCTTTATCTGTAGAAGAGCTCAAAGCTGTAACGATGCAAA
CAGTCGGAGAGAATATTCGTATAAGCCGCGCGCTATATACACCTGTTAACTCTAATCAGAGTGTAGGAATCTATTCTCAT
GGAAATGGGAAAGCCGTTGCCCTGGTCTTTCTTTCCGGATCTGACAAACAAGAAGCGTTAGCAAAAGATATTGCTATGCA
TATTGTTGCAAGTCAGCCACAGTTCTTAAGTAAAGAAAGCGTTCCTCAAGAAGTTTTAGAGAGAGAAAGAGAAGTATTTT
CTTCCCAACTATCAGGAAAACCCCAGGAAGTAATTGAGAAAATTACTACAGGGAAATTTAAGGCCTTTTTCCAAGAGACT
TGTTTATTAGAACAAGCGTTCATTAAGGATCCTGACGTTACTATTCAGGAATTGGTTGATAGAGCTGCAAAAGCTAGTGG
AGAGCCGCTAAAAGTTGAGCACTTTGTCTTTTGGAAGATAGGCGCATAA

Upstream 100 bases:

>100_bases
TTGTTGAAGGGCTTCCTCTTCCTAACGAAGCTCAGAATGATGCTAATTCTAAAGAAGGTTTCTTAGTTTGGACAGATACT
GATAATAGCGAGGCATTGAG

Downstream 100 bases:

>100_bases
AAACCAAGTGGCGAGATGATGAAAAAACGAGTGAAACGAGTTTTATTCAAGATCTCTGGAGAGGCTCTTTCTGACGCTAG
TTCTAGCGACAAAATCAGTG

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSDFSMETLKSLRQRTGVGLTKCKEALEHAKGNLEDAVVYLRKLGLASAGKKEHRETKEGVIAASVDEHGAAIVEVNVET
DFVANNSVFRTFVTGLLSDILNNKLSDVDALAQVTSSQEPSLSVEELKAVTMQTVGENIRISRALYTPVNSNQSVGIYSH
GNGKAVALVFLSGSDKQEALAKDIAMHIVASQPQFLSKESVPQEVLEREREVFSSQLSGKPQEVIEKITTGKFKAFFQET
CLLEQAFIKDPDVTIQELVDRAAKASGEPLKVEHFVFWKIGA

Sequences:

>Translated_282_residues
MSDFSMETLKSLRQRTGVGLTKCKEALEHAKGNLEDAVVYLRKLGLASAGKKEHRETKEGVIAASVDEHGAAIVEVNVET
DFVANNSVFRTFVTGLLSDILNNKLSDVDALAQVTSSQEPSLSVEELKAVTMQTVGENIRISRALYTPVNSNQSVGIYSH
GNGKAVALVFLSGSDKQEALAKDIAMHIVASQPQFLSKESVPQEVLEREREVFSSQLSGKPQEVIEKITTGKFKAFFQET
CLLEQAFIKDPDVTIQELVDRAAKASGEPLKVEHFVFWKIGA
>Mature_281_residues
SDFSMETLKSLRQRTGVGLTKCKEALEHAKGNLEDAVVYLRKLGLASAGKKEHRETKEGVIAASVDEHGAAIVEVNVETD
FVANNSVFRTFVTGLLSDILNNKLSDVDALAQVTSSQEPSLSVEELKAVTMQTVGENIRISRALYTPVNSNQSVGIYSHG
NGKAVALVFLSGSDKQEALAKDIAMHIVASQPQFLSKESVPQEVLEREREVFSSQLSGKPQEVIEKITTGKFKAFFQETC
LLEQAFIKDPDVTIQELVDRAAKASGEPLKVEHFVFWKIGA

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI171846268, Length=220, Percent_Identity=30, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI291084500, Length=241, Percent_Identity=29.8755186721992, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI291084502, Length=93, Percent_Identity=40.8602150537634, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI291084498, Length=93, Percent_Identity=40.8602150537634, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1786366, Length=266, Percent_Identity=37.218045112782, Blast_Score=150, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17561440, Length=288, Percent_Identity=27.0833333333333, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921466, Length=265, Percent_Identity=27.1698113207547, Blast_Score=74, Evalue=9e-14,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_CHLMU (P71146)

Other databases:

- EMBL:   U60196
- EMBL:   AE002160
- PIR:   A81747
- RefSeq:   NP_296434.1
- ProteinModelPortal:   P71146
- SMR:   P71146
- GeneID:   1245578
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0050
- TIGR:   TC_0050
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- ProtClustDB:   PRK09377
- BioCyc:   CMUR243161:TC_0050-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 30824; Mature: 30693

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFSMETLKSLRQRTGVGLTKCKEALEHAKGNLEDAVVYLRKLGLASAGKKEHRETKEG
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHCC
VIAASVDEHGAAIVEVNVETDFVANNSVFRTFVTGLLSDILNNKLSDVDALAQVTSSQEP
EEEEECCCCCCEEEEEECCCCEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
SLSVEELKAVTMQTVGENIRISRALYTPVNSNQSVGIYSHGNGKAVALVFLSGSDKQEAL
CCCHHHHHHHHHHHHCCCEEEEHEEECCCCCCCCEEEEECCCCCEEEEEEEECCCHHHHH
AKDIAMHIVASQPQFLSKESVPQEVLEREREVFSSQLSGKPQEVIEKITTGKFKAFFQET
HHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
CLLEQAFIKDPDVTIQELVDRAAKASGEPLKVEHFVFWKIGA
HHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEECC
>Mature Secondary Structure 
SDFSMETLKSLRQRTGVGLTKCKEALEHAKGNLEDAVVYLRKLGLASAGKKEHRETKEG
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHCC
VIAASVDEHGAAIVEVNVETDFVANNSVFRTFVTGLLSDILNNKLSDVDALAQVTSSQEP
EEEEECCCCCCEEEEEECCCCEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
SLSVEELKAVTMQTVGENIRISRALYTPVNSNQSVGIYSHGNGKAVALVFLSGSDKQEAL
CCCHHHHHHHHHHHHCCCEEEEHEEECCCCCCCCEEEEECCCCCEEEEEEEECCCHHHHH
AKDIAMHIVASQPQFLSKESVPQEVLEREREVFSSQLSGKPQEVIEKITTGKFKAFFQET
HHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
CLLEQAFIKDPDVTIQELVDRAAKASGEPLKVEHFVFWKIGA
HHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9244380; 10684935