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The map label for this gene is ubiE

Identifier: 161353804

GI number: 161353804

Start: 595791

End: 596504

Strand: Direct

Name: ubiE

Synonym: CPj0515

Alternate gene names: 161353804

Gene position: 595791-596504 (Clockwise)

Preceding gene: 15836045

Following gene: 15836053

Centisome position: 48.57

GC content: 44.68

Gene sequence:

>714_bases
TTGAAAAATTCCGGGAATATTATGGAACCCTCTACCAACAAGCCCGACTGTAAAAAGATCTTCGATTCCATAGCGAGTAA
GTATGATCGCACAAATACAATACTCTCTTTAGGAATGCACCATTTCTGGAATCGCTCTTTGATCCAGATCCTAGGGTCGG
GATACTCTCTCCTGGATCTCTGCGCAGGAACAGGAAAAGTCGCGAAGCGTTATATTGCCGCACACCCTCAAGCATCAGTA
ACTCTCGTCGACTTTTCCTCAGCAATGCTCGACATTGCAAAACAACACCTTCCCCAGGGCTCTTGCTCTTTTATTCATAG
CGATATTAATCAACTGCCCTTGGAGAATCATTCTTATCCCCTAGCAGCGATGGCCTATGGCCTCAGGAACCTCTCGGATC
CACATAAAGCCCTACAAGAAATCTCCCGAGTGCTTATGCCTTCTGGAAAACTGGGCATTCTAGAGCTCACACCTCCAAAA
AAAACACACCCTACCTATAGTGCCCATAAGCTCTATTTGCGTGCTGTCGTCCCCTGGATTGGAAAGTCTGTTTCTAAAGA
TCCCGACGCCTATAGCTATCTCAGCAAAAGTATCCAGCAACTTCCAAAGGACCACGATCTTGAAGACCTATTCTCTAAAT
CAGGATTTTATATTGCGAAAAAGAAAAAATTGTTCCTAGGAGCGGCTACGATTTGGCTACTAGAGAAACAATAA

Upstream 100 bases:

>100_bases
CTTAAAGAAGCTCATCAACATACAGGTCTGCCCCCTTCTCTTCTTCAAGAATACTATGCCCTATGCCAGTACCGTCTAGG
AGAAGAACACTACGAAAGCT

Downstream 100 bases:

>100_bases
AACATGGAGGATCTCCAAGCTTGGTCGCAAGGAGCTCCCTAAGATTCGTGGAGTGCTCTACCCCACGCTAAACTAAAATG
GTAAACCTACCTCTGGGAAG

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MKNSGNIMEPSTNKPDCKKIFDSIASKYDRTNTILSLGMHHFWNRSLIQILGSGYSLLDLCAGTGKVAKRYIAAHPQASV
TLVDFSSAMLDIAKQHLPQGSCSFIHSDINQLPLENHSYPLAAMAYGLRNLSDPHKALQEISRVLMPSGKLGILELTPPK
KTHPTYSAHKLYLRAVVPWIGKSVSKDPDAYSYLSKSIQQLPKDHDLEDLFSKSGFYIAKKKKLFLGAATIWLLEKQ

Sequences:

>Translated_237_residues
MKNSGNIMEPSTNKPDCKKIFDSIASKYDRTNTILSLGMHHFWNRSLIQILGSGYSLLDLCAGTGKVAKRYIAAHPQASV
TLVDFSSAMLDIAKQHLPQGSCSFIHSDINQLPLENHSYPLAAMAYGLRNLSDPHKALQEISRVLMPSGKLGILELTPPK
KTHPTYSAHKLYLRAVVPWIGKSVSKDPDAYSYLSKSIQQLPKDHDLEDLFSKSGFYIAKKKKLFLGAATIWLLEKQ
>Mature_237_residues
MKNSGNIMEPSTNKPDCKKIFDSIASKYDRTNTILSLGMHHFWNRSLIQILGSGYSLLDLCAGTGKVAKRYIAAHPQASV
TLVDFSSAMLDIAKQHLPQGSCSFIHSDINQLPLENHSYPLAAMAYGLRNLSDPHKALQEISRVLMPSGKLGILELTPPK
KTHPTYSAHKLYLRAVVPWIGKSVSKDPDAYSYLSKSIQQLPKDHDLEDLFSKSGFYIAKKKKLFLGAATIWLLEKQ

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=254, Percent_Identity=28.3464566929134, Blast_Score=108, Evalue=4e-24,
Organism=Escherichia coli, GI48994983, Length=204, Percent_Identity=32.843137254902, Blast_Score=125, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17557039, Length=221, Percent_Identity=30.316742081448, Blast_Score=127, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6323526, Length=243, Percent_Identity=26.3374485596708, Blast_Score=93, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24641702, Length=245, Percent_Identity=27.3469387755102, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.-

Molecular weight: Translated: 26383; Mature: 26383

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS01183 UBIE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSGNIMEPSTNKPDCKKIFDSIASKYDRTNTILSLGMHHFWNRSLIQILGSGYSLLDL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
CAGTGKVAKRYIAAHPQASVTLVDFSSAMLDIAKQHLPQGSCSFIHSDINQLPLENHSYP
HCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCH
LAAMAYGLRNLSDPHKALQEISRVLMPSGKLGILELTPPKKTHPTYSAHKLYLRAVVPWI
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
GKSVSKDPDAYSYLSKSIQQLPKDHDLEDLFSKSGFYIAKKKKLFLGAATIWLLEKQ
CCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEECCHHEEEHEEEEEEECC
>Mature Secondary Structure
MKNSGNIMEPSTNKPDCKKIFDSIASKYDRTNTILSLGMHHFWNRSLIQILGSGYSLLDL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
CAGTGKVAKRYIAAHPQASVTLVDFSSAMLDIAKQHLPQGSCSFIHSDINQLPLENHSYP
HCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCH
LAAMAYGLRNLSDPHKALQEISRVLMPSGKLGILELTPPKKTHPTYSAHKLYLRAVVPWI
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
GKSVSKDPDAYSYLSKSIQQLPKDHDLEDLFSKSGFYIAKKKKLFLGAATIWLLEKQ
CCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEEECCHHEEEHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]