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The map label for this gene is pheS

Identifier: 15836524

GI number: 15836524

Start: 1137420

End: 1138439

Strand: Direct

Name: pheS

Synonym: CPj0993

Alternate gene names: 15836524

Gene position: 1137420-1138439 (Clockwise)

Preceding gene: 15836523

Following gene: 15836525

Centisome position: 92.73

GC content: 39.61

Gene sequence:

>1020_bases
ATGGAAATGAAAGAAGAGATTGAAGCTGTAAAGCAGCAATTTCACTCTGAGTTAGATCAGGTGAACTCTTCTCAGGCACT
TGCAGATCTTAAGGTCCGCTACTTAGGAAAAAAAGGAATTTTTCGGAGTTTCTCAGAAAAATTAAAGCAATGTACAGATA
AGGCAAAACTAGGTTCCCTTATCAACGACTTTAAAACTTATGTAGAGGATCTTTTACAAGAAAAAAGCCTTGTGCTTCTT
GCTTCAGAACAAGCCGAAGCCTTTTCTAAAGAAAAAATAGATAGCTCTCTCCCTGGAGATTCTCAACCTTCTGGGGGAAG
ACACATTTTAAAAAGTATCCTCGATGATGTTGTCGATATTTTTGTTCACCTAGGATTTTGTGTTCGAGAAGCCCCTAACA
TCGAAAGTGAAGCCAATAATTTTACCTTACTCAACTTCACTGAAGATCATCCTGCTAGACAGATGCATGATACTTTCTAT
CTAAATGCTACAACAGTACTGCGAACACACACATCAAACGTACAGGCTCGTGAACTAAAGAAACAACAACCTCCAATCAA
AGTTGTTGCCCCCGGCCTATGTTTTCGTAACGAAGATATTTCTGCACGTTCTCATGTGCTCTTCCATCAAGTGGAAGCTT
TTTATGTAGATCACAATGTAACGTTTTCGGACTTGACTGCAATCCTCAGTGCATTCTACCATTCCTTCTTCCAAAGGAAA
ACAGAGTTACGTTTTAGACATAGCTACTTTCCTTTTGTTGAGCCAGGTATAGAAGTAGATGTCTCTTGTGAATGTTGTGG
CAAAGGATGTGCCCTTTGCAAACATACAGGTTGGCTGGAAGTTGCGGGAGCGGGAATGATTCATCCCCAAGTACTACGTA
ACGGAAATGTTGATCCTGAAATCTATTCTGGGTATGCTGTTGGCATGGGAATCGAAAGATTGGCAATGTTAAAATATGGC
GTCTCCGATATCCGACTTTTTAGTGAAAACGATTTAAGGTTTTTACAACAATTCTCTTAA

Upstream 100 bases:

>100_bases
TGCTTTCAGAAATAGCTATCCACAATCCTGAAGGTTTTGCAGAAATTGCAAACCAGGCTAAAAAAGCTTTGGAAGCCACA
GTTTAGGGATTAGAATTTCT

Downstream 100 bases:

>100_bases
GGAAGAATGGCAGAGCGGTTTAATGCACCTGTCTTGAAAACAGGAGACCTGAAAGGGTCCGGGGGTTCGAATCCCTCTTC
TTCCGTTTCCTTTTTATTCA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MEMKEEIEAVKQQFHSELDQVNSSQALADLKVRYLGKKGIFRSFSEKLKQCTDKAKLGSLINDFKTYVEDLLQEKSLVLL
ASEQAEAFSKEKIDSSLPGDSQPSGGRHILKSILDDVVDIFVHLGFCVREAPNIESEANNFTLLNFTEDHPARQMHDTFY
LNATTVLRTHTSNVQARELKKQQPPIKVVAPGLCFRNEDISARSHVLFHQVEAFYVDHNVTFSDLTAILSAFYHSFFQRK
TELRFRHSYFPFVEPGIEVDVSCECCGKGCALCKHTGWLEVAGAGMIHPQVLRNGNVDPEIYSGYAVGMGIERLAMLKYG
VSDIRLFSENDLRFLQQFS

Sequences:

>Translated_339_residues
MEMKEEIEAVKQQFHSELDQVNSSQALADLKVRYLGKKGIFRSFSEKLKQCTDKAKLGSLINDFKTYVEDLLQEKSLVLL
ASEQAEAFSKEKIDSSLPGDSQPSGGRHILKSILDDVVDIFVHLGFCVREAPNIESEANNFTLLNFTEDHPARQMHDTFY
LNATTVLRTHTSNVQARELKKQQPPIKVVAPGLCFRNEDISARSHVLFHQVEAFYVDHNVTFSDLTAILSAFYHSFFQRK
TELRFRHSYFPFVEPGIEVDVSCECCGKGCALCKHTGWLEVAGAGMIHPQVLRNGNVDPEIYSGYAVGMGIERLAMLKYG
VSDIRLFSENDLRFLQQFS
>Mature_339_residues
MEMKEEIEAVKQQFHSELDQVNSSQALADLKVRYLGKKGIFRSFSEKLKQCTDKAKLGSLINDFKTYVEDLLQEKSLVLL
ASEQAEAFSKEKIDSSLPGDSQPSGGRHILKSILDDVVDIFVHLGFCVREAPNIESEANNFTLLNFTEDHPARQMHDTFY
LNATTVLRTHTSNVQARELKKQQPPIKVVAPGLCFRNEDISARSHVLFHQVEAFYVDHNVTFSDLTAILSAFYHSFFQRK
TELRFRHSYFPFVEPGIEVDVSCECCGKGCALCKHTGWLEVAGAGMIHPQVLRNGNVDPEIYSGYAVGMGIERLAMLKYG
VSDIRLFSENDLRFLQQFS

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=270, Percent_Identity=30.3703703703704, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI5729820, Length=240, Percent_Identity=30, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI1788007, Length=325, Percent_Identity=43.3846153846154, Blast_Score=278, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI17508957, Length=270, Percent_Identity=28.5185185185185, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI32563657, Length=270, Percent_Identity=28.5185185185185, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32566635, Length=249, Percent_Identity=28.1124497991968, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6321087, Length=374, Percent_Identity=26.7379679144385, Blast_Score=109, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6325304, Length=101, Percent_Identity=33.6633663366337, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI18858079, Length=379, Percent_Identity=25.3298153034301, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI17137424, Length=248, Percent_Identity=27.0161290322581, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_CHLPN (Q9Z6R6)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B72011
- PIR:   F86614
- RefSeq:   NP_225187.1
- RefSeq:   NP_301048.1
- RefSeq:   NP_445400.1
- RefSeq:   NP_877303.1
- ProteinModelPortal:   Q9Z6R6
- SMR:   Q9Z6R6
- GeneID:   1467710
- GeneID:   895511
- GeneID:   919761
- GeneID:   963308
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0862
- KEGG:   cpn:CPn0993
- KEGG:   cpt:CpB1031
- TIGR:   CP_0862
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   CPNE115711:CP_0862-MONOMER
- BioCyc:   CPNE115713:CPN0993-MONOMER
- BioCyc:   CPNE138677:CPJ0993-MONOMER
- BioCyc:   CPNE182082:CPB1031-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38367; Mature: 38367

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMKEEIEAVKQQFHSELDQVNSSQALADLKVRYLGKKGIFRSFSEKLKQCTDKAKLGSL
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
INDFKTYVEDLLQEKSLVLLASEQAEAFSKEKIDSSLPGDSQPSGGRHILKSILDDVVDI
HHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
FVHLGFCVREAPNIESEANNFTLLNFTEDHPARQMHDTFYLNATTVLRTHTSNVQARELK
HHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHCEEEEEHEEEEEECCCCCHHHHHH
KQQPPIKVVAPGLCFRNEDISARSHVLFHQVEAFYVDHNVTFSDLTAILSAFYHSFFQRK
HCCCCEEEEECCCEECCCCCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHH
TELRFRHSYFPFVEPGIEVDVSCECCGKGCALCKHTGWLEVAGAGMIHPQVLRNGNVDPE
HHHHHHHCCCCCCCCCCCEEEEHHHCCCCCHHHHCCCCEEECCCCCCCHHHHHCCCCCCH
IYSGYAVGMGIERLAMLKYGVSDIRLFSENDLRFLQQFS
HHCCEEHHHHHHHHHHHHHCHHHEEEECCCHHHHHHHCC
>Mature Secondary Structure
MEMKEEIEAVKQQFHSELDQVNSSQALADLKVRYLGKKGIFRSFSEKLKQCTDKAKLGSL
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
INDFKTYVEDLLQEKSLVLLASEQAEAFSKEKIDSSLPGDSQPSGGRHILKSILDDVVDI
HHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
FVHLGFCVREAPNIESEANNFTLLNFTEDHPARQMHDTFYLNATTVLRTHTSNVQARELK
HHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHCEEEEEHEEEEEECCCCCHHHHHH
KQQPPIKVVAPGLCFRNEDISARSHVLFHQVEAFYVDHNVTFSDLTAILSAFYHSFFQRK
HCCCCEEEEECCCEECCCCCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHH
TELRFRHSYFPFVEPGIEVDVSCECCGKGCALCKHTGWLEVAGAGMIHPQVLRNGNVDPE
HHHHHHHCCCCCCCCCCCEEEEHHHCCCCCHHHHCCCCEEECCCCCCCHHHHHCCCCCCH
IYSGYAVGMGIERLAMLKYGVSDIRLFSENDLRFLQQFS
HHCCEEHHHHHHHHHHHHHCHHHEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362