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The map label for this gene is pckA

Identifier: 15836384

GI number: 15836384

Start: 965717

End: 967519

Strand: Direct

Name: pckA

Synonym: CPj0851

Alternate gene names: 15836384

Gene position: 965717-967519 (Clockwise)

Preceding gene: 15836383

Following gene: 15836385

Centisome position: 78.73

GC content: 43.76

Gene sequence:

>1803_bases
ATGGTATGGAGCACCAACATCAAGCATGAAGGTCTGAAATCTTGGATTGATGAGGTTGCTAAATTAACGACTCCGAAGGA
CATACGTCTTTGTGATGGTTCGGATACCGAGTATGATGAGCTCTGCACTCTTATGGAGAGTACAGGGACGATGATCCGTT
TGAATCCTGAGTTTCATCCCAATTGCTTTTTGGTCCGTTCGTCTGCTGATGATGTCGCTCGGGTAGAGCAATTTACTTTC
ATTTGCACTTCGACGGAAGCAGAGGCAGGCCCTACGAATAATTGGAGAGATCCTCAAGAGATGCGTAGGGAGTTGCATCA
ACTTTTTCGTGGATGTATGCAGGGGCGCACTCTGTATATAGTTCCTTTCTGTATGGGTCCTTTAGACTCTCCATTTTCTA
TTGTAGGTGTTGAGTTAACAGATTCTCCTTATGTCGTTTGTTCTATGAAGATCATGACTCGTATGGGTGATGATGTTTTA
CGTTCTTTAGGGACTTCGGGGAAGTTTTTAAAGTGTTTACATAGTGTGGGTAAGCCTTTATCTCCAGGAGAGGCCGATGT
TTCTTGGCCTTGCAATCCGAAATCAATGCGGATTGTACATTTTCAAGATGATAGTAGTGTGATGTCTTTTGGAAGTGGTT
ATGGAGGCAATGCTTTACTGGGTAAGAAGTGTGTGGCTCTTCGCTTAGCTTCTTACATGGCAAAATCTCAGGGCTGGCTT
GCTGAGCACATGTTGATTATTGGAATTACCAATCCTGAAGGGAAGAAAAAATACTTTTCTGCTTCGTTCCCGAGTGCCTG
TGGTAAGACAAATTTAGCTATGTTGATGCCTAAACTTCCAGGTTGGAAGATTGAGTGTATTGGGGATGATATTGCTTGGA
TCCGTCCGGGTCGTGACGGCAGGTTGTATGCTGTAAATCCAGAATATGGATTTTTTGGTGTTGCTCCTGGGACTTCTGAG
CGTACAAATCCCAATGCTTTGGCAACTTGCAGGTCGAATTCTATTTTTACGAATGTTGCCTTGACTGCCGATGGGGATGT
TTGGTGGGAGGGATTAACGGAGCAACCACCAGAGCCTTTAACAGATTGGCTTGGAAAGCCTTGGAAGCCTGGAGGGAGTC
CTGCTGCCCATCCGAATTCTCGATTTACAGCTCCTTTACGTCAGTGTCCTTCTTTAGATCCTGAGTGGAATAGTCCTCAG
GGTGTTCCCTTAGATGCTATTATTTTTGGCGGACGTCGTTCCGAAACCATTCCTTTAGTTTACGAAGCTTTAAGTTGGGA
ACACGGAGTGACTATAGGAGCGGGGATGTCATCGACGACAACTGCAGCTATTGTTGGTCAGTTAGGTAAACTCCGCCACG
ATCCTTTTGCCATGCTTCCTTTCTGTGGGTATAACATGGCATATTACTTCCAGCATTGGCTTTCCTTTGCTGAGAATAGA
AGTCTTAAGCTTCCTAAGATTTTTGGAGTCAATTGGTTCCGTAAGAATAATCAAGGGGAGTTTCTCTGGCCTGGTTTTAG
TGAAAACTTACGTGTTCTAGAATGGATATTCCAAAGGACTGATGGTCTGGAAGATATTGCAGAGCGCACGCCTATTGGTT
ATCTTCCTAACATTCAAAAATTCAACCTCAATGGCTTGAATCTTGACCTACAAACTGTACAAGAACTGTTCTCTGTAGAT
GCTGAGGGTTGGCTTGCTGAAGTTGAGAACATTGGGGAGTATTTGAAGATCTTTGGTTCGGATTGTCCGCAGCAGATTAC
TGATGAGTTGTTGCGAATTAAATCAGAATTAAAAGAAAAATAA

Upstream 100 bases:

>100_bases
TTACAGCACCGCATCCTTTGCTGGCAGTTTGTTTAGGAACCGGGAAAGCTTTGGAACATTTAGATCAGTTTAAGAAGCGT
AAAGGGAATTTGGTATAGTC

Downstream 100 bases:

>100_bases
AAATCAAAGTCATTAGTTATTTATAAGTTAGTTTTAATAACAATTATTTCTTATTTTTTATAAATTATTTTTTAAATAAA
ATTAAGAATAATTGATTATT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MVWSTNIKHEGLKSWIDEVAKLTTPKDIRLCDGSDTEYDELCTLMESTGTMIRLNPEFHPNCFLVRSSADDVARVEQFTF
ICTSTEAEAGPTNNWRDPQEMRRELHQLFRGCMQGRTLYIVPFCMGPLDSPFSIVGVELTDSPYVVCSMKIMTRMGDDVL
RSLGTSGKFLKCLHSVGKPLSPGEADVSWPCNPKSMRIVHFQDDSSVMSFGSGYGGNALLGKKCVALRLASYMAKSQGWL
AEHMLIIGITNPEGKKKYFSASFPSACGKTNLAMLMPKLPGWKIECIGDDIAWIRPGRDGRLYAVNPEYGFFGVAPGTSE
RTNPNALATCRSNSIFTNVALTADGDVWWEGLTEQPPEPLTDWLGKPWKPGGSPAAHPNSRFTAPLRQCPSLDPEWNSPQ
GVPLDAIIFGGRRSETIPLVYEALSWEHGVTIGAGMSSTTTAAIVGQLGKLRHDPFAMLPFCGYNMAYYFQHWLSFAENR
SLKLPKIFGVNWFRKNNQGEFLWPGFSENLRVLEWIFQRTDGLEDIAERTPIGYLPNIQKFNLNGLNLDLQTVQELFSVD
AEGWLAEVENIGEYLKIFGSDCPQQITDELLRIKSELKEK

Sequences:

>Translated_600_residues
MVWSTNIKHEGLKSWIDEVAKLTTPKDIRLCDGSDTEYDELCTLMESTGTMIRLNPEFHPNCFLVRSSADDVARVEQFTF
ICTSTEAEAGPTNNWRDPQEMRRELHQLFRGCMQGRTLYIVPFCMGPLDSPFSIVGVELTDSPYVVCSMKIMTRMGDDVL
RSLGTSGKFLKCLHSVGKPLSPGEADVSWPCNPKSMRIVHFQDDSSVMSFGSGYGGNALLGKKCVALRLASYMAKSQGWL
AEHMLIIGITNPEGKKKYFSASFPSACGKTNLAMLMPKLPGWKIECIGDDIAWIRPGRDGRLYAVNPEYGFFGVAPGTSE
RTNPNALATCRSNSIFTNVALTADGDVWWEGLTEQPPEPLTDWLGKPWKPGGSPAAHPNSRFTAPLRQCPSLDPEWNSPQ
GVPLDAIIFGGRRSETIPLVYEALSWEHGVTIGAGMSSTTTAAIVGQLGKLRHDPFAMLPFCGYNMAYYFQHWLSFAENR
SLKLPKIFGVNWFRKNNQGEFLWPGFSENLRVLEWIFQRTDGLEDIAERTPIGYLPNIQKFNLNGLNLDLQTVQELFSVD
AEGWLAEVENIGEYLKIFGSDCPQQITDELLRIKSELKEK
>Mature_600_residues
MVWSTNIKHEGLKSWIDEVAKLTTPKDIRLCDGSDTEYDELCTLMESTGTMIRLNPEFHPNCFLVRSSADDVARVEQFTF
ICTSTEAEAGPTNNWRDPQEMRRELHQLFRGCMQGRTLYIVPFCMGPLDSPFSIVGVELTDSPYVVCSMKIMTRMGDDVL
RSLGTSGKFLKCLHSVGKPLSPGEADVSWPCNPKSMRIVHFQDDSSVMSFGSGYGGNALLGKKCVALRLASYMAKSQGWL
AEHMLIIGITNPEGKKKYFSASFPSACGKTNLAMLMPKLPGWKIECIGDDIAWIRPGRDGRLYAVNPEYGFFGVAPGTSE
RTNPNALATCRSNSIFTNVALTADGDVWWEGLTEQPPEPLTDWLGKPWKPGGSPAAHPNSRFTAPLRQCPSLDPEWNSPQ
GVPLDAIIFGGRRSETIPLVYEALSWEHGVTIGAGMSSTTTAAIVGQLGKLRHDPFAMLPFCGYNMAYYFQHWLSFAENR
SLKLPKIFGVNWFRKNNQGEFLWPGFSENLRVLEWIFQRTDGLEDIAERTPIGYLPNIQKFNLNGLNLDLQTVQELFSVD
AEGWLAEVENIGEYLKIFGSDCPQQITDELLRIKSELKEK

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family

Homologues:

Organism=Homo sapiens, GI66346721, Length=604, Percent_Identity=53.476821192053, Blast_Score=648, Evalue=0.0,
Organism=Homo sapiens, GI187281517, Length=603, Percent_Identity=52.4046434494196, Blast_Score=629, Evalue=1e-180,
Organism=Homo sapiens, GI66346723, Length=374, Percent_Identity=56.1497326203209, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71989645, Length=597, Percent_Identity=49.2462311557789, Blast_Score=580, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI17555492, Length=612, Percent_Identity=47.7124183006536, Blast_Score=580, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI71989658, Length=597, Percent_Identity=49.2462311557789, Blast_Score=578, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI71989653, Length=584, Percent_Identity=49.6575342465753, Blast_Score=572, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI32564411, Length=537, Percent_Identity=49.5344506517691, Blast_Score=548, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI32564407, Length=539, Percent_Identity=49.721706864564, Blast_Score=545, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI71989649, Length=501, Percent_Identity=49.500998003992, Blast_Score=496, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI32564409, Length=367, Percent_Identity=51.4986376021798, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17540956, Length=614, Percent_Identity=25.2442996742671, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24655085, Length=600, Percent_Identity=51, Blast_Score=602, Evalue=1e-172,
Organism=Drosophila melanogaster, GI24655082, Length=604, Percent_Identity=50.8278145695364, Blast_Score=601, Evalue=1e-172,
Organism=Drosophila melanogaster, GI161077226, Length=570, Percent_Identity=52.4561403508772, Blast_Score=580, Evalue=1e-165,
Organism=Drosophila melanogaster, GI24655088, Length=501, Percent_Identity=54.0918163672655, Blast_Score=544, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCKG_CHLPN (Q9Z755)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   A86597
- PIR:   E72027
- RefSeq:   NP_225046.1
- RefSeq:   NP_300908.1
- RefSeq:   NP_445555.1
- RefSeq:   NP_877152.1
- ProteinModelPortal:   Q9Z755
- SMR:   Q9Z755
- PHCI-2DPAGE:   Q9Z755
- GeneID:   1467559
- GeneID:   895150
- GeneID:   919614
- GeneID:   963494
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP1018
- KEGG:   cpn:CPn0851
- KEGG:   cpt:CpB0880
- TIGR:   CP_1018
- HOGENOM:   HBG484437
- OMA:   CGYHMGD
- ProtClustDB:   PRK04210
- BioCyc:   CPNE115711:CP_1018-MONOMER
- BioCyc:   CPNE115713:CPN0851-MONOMER
- BioCyc:   CPNE138677:CPJ0851-MONOMER
- BioCyc:   CPNE182082:CPB0880-MONOMER
- BRENDA:   4.1.1.32
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00452
- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PANTHER:   PTHR11561
- PIRSF:   PIRSF001348

Pfam domain/function: PF00821 PEPCK; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.32

Molecular weight: Translated: 66992; Mature: 66992

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00505 PEPCK_GTP; PS00012 PHOSPHOPANTETHEINE

Important sites: ACT_SITE 267-267 BINDING 74-74 BINDING 216-216 BINDING 223-223 BINDING 265-265 BINDING 381-381 BINDING 412-412

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVWSTNIKHEGLKSWIDEVAKLTTPKDIRLCDGSDTEYDELCTLMESTGTMIRLNPEFHP
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCC
NCFLVRSSADDVARVEQFTFICTSTEAEAGPTNNWRDPQEMRRELHQLFRGCMQGRTLYI
CEEEEECCCHHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEE
VPFCMGPLDSPFSIVGVELTDSPYVVCSMKIMTRMGDDVLRSLGTSGKFLKCLHSVGKPL
EEEECCCCCCCCEEEEEEECCCCEEEEHHHHHHHCCHHHHHHHCCCCHHHHHHHHHCCCC
SPGEADVSWPCNPKSMRIVHFQDDSSVMSFGSGYGGNALLGKKCVALRLASYMAKSQGWL
CCCCCCCCCCCCCCCEEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
AEHMLIIGITNPEGKKKYFSASFPSACGKTNLAMLMPKLPGWKIECIGDDIAWIRPGRDG
EEEEEEEEECCCCCCCCEECCCCCCCCCCCCCEEEECCCCCCEEEEECCCEEEECCCCCC
RLYAVNPEYGFFGVAPGTSERTNPNALATCRSNSIFTNVALTADGDVWWEGLTEQPPEPL
EEEEECCCCCEEECCCCCCCCCCCCEEEEECCCCEEEEEEEEECCCHHCCCCCCCCCCHH
TDWLGKPWKPGGSPAAHPNSRFTAPLRQCPSLDPEWNSPQGVPLDAIIFGGRRSETIPLV
HHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHH
YEALSWEHGVTIGAGMSSTTTAAIVGQLGKLRHDPFAMLPFCGYNMAYYFQHWLSFAENR
HHHHCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCC
SLKLPKIFGVNWFRKNNQGEFLWPGFSENLRVLEWIFQRTDGLEDIAERTPIGYLPNIQK
CCCCCEEECCEEEEECCCCCEECCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCE
FNLNGLNLDLQTVQELFSVDAEGWLAEVENIGEYLKIFGSDCPQQITDELLRIKSELKEK
ECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVWSTNIKHEGLKSWIDEVAKLTTPKDIRLCDGSDTEYDELCTLMESTGTMIRLNPEFHP
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCC
NCFLVRSSADDVARVEQFTFICTSTEAEAGPTNNWRDPQEMRRELHQLFRGCMQGRTLYI
CEEEEECCCHHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEE
VPFCMGPLDSPFSIVGVELTDSPYVVCSMKIMTRMGDDVLRSLGTSGKFLKCLHSVGKPL
EEEECCCCCCCCEEEEEEECCCCEEEEHHHHHHHCCHHHHHHHCCCCHHHHHHHHHCCCC
SPGEADVSWPCNPKSMRIVHFQDDSSVMSFGSGYGGNALLGKKCVALRLASYMAKSQGWL
CCCCCCCCCCCCCCCEEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
AEHMLIIGITNPEGKKKYFSASFPSACGKTNLAMLMPKLPGWKIECIGDDIAWIRPGRDG
EEEEEEEEECCCCCCCCEECCCCCCCCCCCCCEEEECCCCCCEEEEECCCEEEECCCCCC
RLYAVNPEYGFFGVAPGTSERTNPNALATCRSNSIFTNVALTADGDVWWEGLTEQPPEPL
EEEEECCCCCEEECCCCCCCCCCCCEEEEECCCCEEEEEEEEECCCHHCCCCCCCCCCHH
TDWLGKPWKPGGSPAAHPNSRFTAPLRQCPSLDPEWNSPQGVPLDAIIFGGRRSETIPLV
HHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHH
YEALSWEHGVTIGAGMSSTTTAAIVGQLGKLRHDPFAMLPFCGYNMAYYFQHWLSFAENR
HHHHCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCC
SLKLPKIFGVNWFRKNNQGEFLWPGFSENLRVLEWIFQRTDGLEDIAERTPIGYLPNIQK
CCCCCEEECCEEEEECCCCCEECCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCE
FNLNGLNLDLQTVQELFSVDAEGWLAEVENIGEYLKIFGSDCPQQITDELLRIKSELKEK
ECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362