Click here to switch to the map view.

The map label for this gene is brnQ

Identifier: 15836369

GI number: 15836369

Start: 945040

End: 946200

Strand: Reverse

Name: brnQ

Synonym: CPj0836

Alternate gene names: 15836369

Gene position: 946200-945040 (Counterclockwise)

Preceding gene: 15836370

Following gene: 15836368

Centisome position: 77.14

GC content: 40.31

Gene sequence:

>1161_bases
ATGTTCTTCGGAGCTGGCAATATTGTCTTTCCCTTAGCTCTGGGATACCACTACAATGCGCATCCCTGGTCTGCCTATTT
CGGCATGATGCTTACTGCTGTATGCGTTCCTCTTTTAGGTCTAGTCAGTATGCTATTCTATTCTGGAGACTACCAGAAGT
TCTTTTTCTCGATAGGAAGAATTCCAGGGATGATTTTTATTACGGCTATTATACTTTTGATAGGCCCCTTCGGTGGAATC
CCTCGAGCCATTGCAGTATCTCATGCCACATTGATTTCACTATCCGAGCATAAGAGTGCTTTCATTCCCTCTTTGCCTAT
TTTTAGTGCGATTTGCTGTGTGCTTATCTATATCTTCTCATGCAAGCTCAGCCGTTTAATTCAATGGCTAGGATCTGTAT
TTTTCCCGATTATGTTGGTTACCTTACTTTGGGTCATCATCCGCAGTTTTATGATTCCTACCCACCCCATGGTTCAAGAA
TTTATTCCGAACGCACGTCAAGCTTGGTTGGCAGGATTCATTGAAGGGTTCAATACTATGGATTTACTTGCAGCGTTTTT
CTTCTGCTCCATAGTTTTGATCTCTCTACGTCAGCTCGTAGCTGAAGAGAAACATCCTACAGAGGAAGAAATCCCTCTTA
GTTTTCAAGGTATTAGCAAAAAAAATAAGCGTTCCTTAGCTTTAGGTTTCTTTCTTGCTGCGATCTTACTAGGAATGACC
TATCTTGGATTTGTTTTATCTGCTGCACGTCATGCGGGCTTACTTGTGAATGTAAGTAAAGGTCATATTCTGGGAAGGAT
CTCTGCGATTGCATTAGGGCCAAATAGCATTCTCGCAGGAGTCAGCGTTTTCATTGCCTGTCTAACTACGGAAATTGCTT
TAGTAGGTATTGTTGCAGATTTTCTAGCTCGTGTAGTTTCCTTTAAGAAACTGAACTATGCTTCAGCAGTAATTTGTACT
CTTATCCCCACCTATTTAATTTCTATTTTGAATTTTGAAACTATAAGTCACCTACTCTTGCCTTTGCTACAACTTAGCTA
TCCAGCTTTGATTGTACTGGCTTGTGGAAACATTGCCTACAAGTTATGGAATTTCCGATACTCCCCAGTCTTGTTTTATT
TAACCCTCTCTCTTACAATTGTTTTAAAGTTAGTGAATTAA

Upstream 100 bases:

>100_bases
CTATCTCTACTTTATTTAAAAGATGAAAAAAAACGCATCTCATAAAACAAATGATAAAAAAAGTTTGTCCATTTGGTCTA
TTGGAGGATCTATTTTTGCT

Downstream 100 bases:

>100_bases
AATAACATACGGGTTGCTAGCATAATATTATGGTTTTAGAAGCATTAGCCATCTTTCGACAGGATGCGATGCAACATCTC
CTTAAACATCGCAAGGAGAT

Product: amino acid (branched) transport

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MFFGAGNIVFPLALGYHYNAHPWSAYFGMMLTAVCVPLLGLVSMLFYSGDYQKFFFSIGRIPGMIFITAIILLIGPFGGI
PRAIAVSHATLISLSEHKSAFIPSLPIFSAICCVLIYIFSCKLSRLIQWLGSVFFPIMLVTLLWVIIRSFMIPTHPMVQE
FIPNARQAWLAGFIEGFNTMDLLAAFFFCSIVLISLRQLVAEEKHPTEEEIPLSFQGISKKNKRSLALGFFLAAILLGMT
YLGFVLSAARHAGLLVNVSKGHILGRISAIALGPNSILAGVSVFIACLTTEIALVGIVADFLARVVSFKKLNYASAVICT
LIPTYLISILNFETISHLLLPLLQLSYPALIVLACGNIAYKLWNFRYSPVLFYLTLSLTIVLKLVN

Sequences:

>Translated_386_residues
MFFGAGNIVFPLALGYHYNAHPWSAYFGMMLTAVCVPLLGLVSMLFYSGDYQKFFFSIGRIPGMIFITAIILLIGPFGGI
PRAIAVSHATLISLSEHKSAFIPSLPIFSAICCVLIYIFSCKLSRLIQWLGSVFFPIMLVTLLWVIIRSFMIPTHPMVQE
FIPNARQAWLAGFIEGFNTMDLLAAFFFCSIVLISLRQLVAEEKHPTEEEIPLSFQGISKKNKRSLALGFFLAAILLGMT
YLGFVLSAARHAGLLVNVSKGHILGRISAIALGPNSILAGVSVFIACLTTEIALVGIVADFLARVVSFKKLNYASAVICT
LIPTYLISILNFETISHLLLPLLQLSYPALIVLACGNIAYKLWNFRYSPVLFYLTLSLTIVLKLVN
>Mature_386_residues
MFFGAGNIVFPLALGYHYNAHPWSAYFGMMLTAVCVPLLGLVSMLFYSGDYQKFFFSIGRIPGMIFITAIILLIGPFGGI
PRAIAVSHATLISLSEHKSAFIPSLPIFSAICCVLIYIFSCKLSRLIQWLGSVFFPIMLVTLLWVIIRSFMIPTHPMVQE
FIPNARQAWLAGFIEGFNTMDLLAAFFFCSIVLISLRQLVAEEKHPTEEEIPLSFQGISKKNKRSLALGFFLAAILLGMT
YLGFVLSAARHAGLLVNVSKGHILGRISAIALGPNSILAGVSVFIACLTTEIALVGIVADFLARVVSFKKLNYASAVICT
LIPTYLISILNFETISHLLLPLLQLSYPALIVLACGNIAYKLWNFRYSPVLFYLTLSLTIVLKLVN

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=368, Percent_Identity=26.9021739130435, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 42519; Mature: 42519

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFGAGNIVFPLALGYHYNAHPWSAYFGMMLTAVCVPLLGLVSMLFYSGDYQKFFFSIGR
CEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
IPGMIFITAIILLIGPFGGIPRAIAVSHATLISLSEHKSAFIPSLPIFSAICCVLIYIFS
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
CKLSRLIQWLGSVFFPIMLVTLLWVIIRSFMIPTHPMVQEFIPNARQAWLAGFIEGFNTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHCCCHH
DLLAAFFFCSIVLISLRQLVAEEKHPTEEEIPLSFQGISKKNKRSLALGFFLAAILLGMT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YLGFVLSAARHAGLLVNVSKGHILGRISAIALGPNSILAGVSVFIACLTTEIALVGIVAD
HHHHHHHHHHHCCEEEEECCCCHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
FLARVVSFKKLNYASAVICTLIPTYLISILNFETISHLLLPLLQLSYPALIVLACGNIAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
KLWNFRYSPVLFYLTLSLTIVLKLVN
HHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFFGAGNIVFPLALGYHYNAHPWSAYFGMMLTAVCVPLLGLVSMLFYSGDYQKFFFSIGR
CEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
IPGMIFITAIILLIGPFGGIPRAIAVSHATLISLSEHKSAFIPSLPIFSAICCVLIYIFS
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
CKLSRLIQWLGSVFFPIMLVTLLWVIIRSFMIPTHPMVQEFIPNARQAWLAGFIEGFNTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHCCCHH
DLLAAFFFCSIVLISLRQLVAEEKHPTEEEIPLSFQGISKKNKRSLALGFFLAAILLGMT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YLGFVLSAARHAGLLVNVSKGHILGRISAIALGPNSILAGVSVFIACLTTEIALVGIVAD
HHHHHHHHHHHCCEEEEECCCCHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
FLARVVSFKKLNYASAVICTLIPTYLISILNFETISHLLLPLLQLSYPALIVLACGNIAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
KLWNFRYSPVLFYLTLSLTIVLKLVN
HHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]