Click here to switch to the map view.

The map label for this gene is gyrB_2

Identifier: 15836247

GI number: 15836247

Start: 800978

End: 802786

Strand: Direct

Name: gyrB_2

Synonym: CPj0715

Alternate gene names: 15836247

Gene position: 800978-802786 (Clockwise)

Preceding gene: 15836233

Following gene: 15836248

Centisome position: 65.3

GC content: 38.09

Gene sequence:

>1809_bases
ATGGCGGCATACACAGAAGCAAGCATTCTTTCTTTGGCCTCTCTTGATCACATTCGTCTTCGGGCGGGGATGTACATTGG
AAGGCTTGGCAATGGTTCTCAAAAAGAGGATGGGATTTACACTCTTTTTAAAGAAGTGGTTGATAATGGGATTGATGAAT
TTATCATGGGTCATGGTAAATCTTTAAAAATTTCTGCTAGTGACAAGCAGATCTCCATTCAAGATCAAGGTCGTGGCATT
CCTTTAGGTAAACTTATAGATTGTGTTTCTAAAATCAATACGGGAGCTAAATATACCCAAGATGTTTTCCATTTCTCTGT
AGGGCTGAATGGCGTGGGACTCAAAGCTGTGAATGCACTTTCAGAAATATTTTCTGTACGTTCTGTAAGAAAGAAAAAAT
ACCACCTTGCCACCTTCCATCGAGGAGTTCTGCAAGAGTCTAAGCAAGGTTCTACCAAAGATCCTGATGGAACTTTTGTT
TCCTTTACTCCTGATCCTAGTATCTTCCCTGAGTTTACTTTTAACCACGACTTCCTAAAAGATAAAATCCGCCAATACAC
CTACCTACATTCGGGATTAGAGATCCGATTTAATGATGAGGTGTTCATATCTCACAACGGTCTCAAAGATCTTTTCGATG
CAGAGATCACTGAGCCCCCTTTATACTCTCCTCTTTTTTTTCAAAATGAGGATTTAACTTTTATCTTTTCTCACCTTGAA
GGAAATACGGAGCGTTATTTTTCTTTTGTCAATGGACAAGAGACTCTTGACGGAGGAACACACCTGACTGCCTTTAAGGA
AGCCATAGTAAAAGGGGTCAACGAGTTTTTTGGAAAAACATTTGTTTCCAATGACATTCGAGAAGGCATTGTGGGCTGCA
TAGCAATAAAAATAGCCTCGCCAATTTTTGAATCGCAAACGAAAAATAAGCTTGGGAATACACAGATTCGGTCTTCTTTA
ATTAAAGATGTAAAGGAAGCGATTGTACAGGCCCTACGTAAAGATAAAGTGGCTCCTGAGCTTCTTTTAGAAAAAATAAA
ATTCAATGAGAAAACTCGAAAGAATATCCAATTTATAAAACAAGATCTTAAGAGCAAACAGAAGAAAGTCCATTATAAAA
TTCCCAAACTTCGGGACTGTAAATTCCATTATAACGATCGCTCTCTGTATGGTGAGGCCTCTTCGATTTTCCTTACCGAA
GGGGAGTCTGCGTCCGCATCAATTCTTGCTTCAAGAAATCCCCTCACACAAGCTGTCTTTTCACTTCGAGGAAAGCCTAT
GAATGTCTTTTCCTTAGAAGAAACCAAAATGTATAAAAATGATGAGTTATTTTATTTAGCAACTGCTCTAGGCATCACGC
AAAACGAGATTCAGCATTTACGTTATAACAAAGTCATCCTGGCTACTGATGCGGATGTAGACGGTATGCATATTCGTAAT
CTTTTGATTACTTTCTTCCTTAAAACACTCTTGCCTCTTGTAGAAAATAATCACCTCTTTATCTTAGAAACCCCTTTGTT
TAAAGTTAGAAACAAAACGACTACGCTCTACTACTATTCTGAGCAAGAAAAGATGCAGGCGTTACAGCAATTTGGGAAAA
AGGACTCCTCTTTAGAAATCACAAGGTTTAAAGGTTTAGGAGAAATTTCTCCTAAGGAATTTGCTGCGTTTATAGGTCCT
GAGATCCGCCTCACCCCAGTTACGATTACCTCTTTAGAGAGCATTTCTTCGATCTTACAATTCTATATGGGGAAAAATAC
AAAAGAGAGAAAACAATTTATTATGGATAACCTTATTACTGATTTTTAA

Upstream 100 bases:

>100_bases
ACTTATATGTTGAGCTTGTCCCTTTTCTTGGCGCAAAATAGATAAGCAATCAAAAGATTGTCCTATCCGCTCTTCTTAAA
AATTCAACAAAATATCACAC

Downstream 100 bases:

>100_bases
TTTATGCGTGACGTTTCAGAGCTTTTTCGAACACATTTTATGCATTACGCGTCTTACGTAATTTTAGAGAGAGCGATTCC
TCATATTCTTGATGGCTTAA

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MAAYTEASILSLASLDHIRLRAGMYIGRLGNGSQKEDGIYTLFKEVVDNGIDEFIMGHGKSLKISASDKQISIQDQGRGI
PLGKLIDCVSKINTGAKYTQDVFHFSVGLNGVGLKAVNALSEIFSVRSVRKKKYHLATFHRGVLQESKQGSTKDPDGTFV
SFTPDPSIFPEFTFNHDFLKDKIRQYTYLHSGLEIRFNDEVFISHNGLKDLFDAEITEPPLYSPLFFQNEDLTFIFSHLE
GNTERYFSFVNGQETLDGGTHLTAFKEAIVKGVNEFFGKTFVSNDIREGIVGCIAIKIASPIFESQTKNKLGNTQIRSSL
IKDVKEAIVQALRKDKVAPELLLEKIKFNEKTRKNIQFIKQDLKSKQKKVHYKIPKLRDCKFHYNDRSLYGEASSIFLTE
GESASASILASRNPLTQAVFSLRGKPMNVFSLEETKMYKNDELFYLATALGITQNEIQHLRYNKVILATDADVDGMHIRN
LLITFFLKTLLPLVENNHLFILETPLFKVRNKTTTLYYYSEQEKMQALQQFGKKDSSLEITRFKGLGEISPKEFAAFIGP
EIRLTPVTITSLESISSILQFYMGKNTKERKQFIMDNLITDF

Sequences:

>Translated_602_residues
MAAYTEASILSLASLDHIRLRAGMYIGRLGNGSQKEDGIYTLFKEVVDNGIDEFIMGHGKSLKISASDKQISIQDQGRGI
PLGKLIDCVSKINTGAKYTQDVFHFSVGLNGVGLKAVNALSEIFSVRSVRKKKYHLATFHRGVLQESKQGSTKDPDGTFV
SFTPDPSIFPEFTFNHDFLKDKIRQYTYLHSGLEIRFNDEVFISHNGLKDLFDAEITEPPLYSPLFFQNEDLTFIFSHLE
GNTERYFSFVNGQETLDGGTHLTAFKEAIVKGVNEFFGKTFVSNDIREGIVGCIAIKIASPIFESQTKNKLGNTQIRSSL
IKDVKEAIVQALRKDKVAPELLLEKIKFNEKTRKNIQFIKQDLKSKQKKVHYKIPKLRDCKFHYNDRSLYGEASSIFLTE
GESASASILASRNPLTQAVFSLRGKPMNVFSLEETKMYKNDELFYLATALGITQNEIQHLRYNKVILATDADVDGMHIRN
LLITFFLKTLLPLVENNHLFILETPLFKVRNKTTTLYYYSEQEKMQALQQFGKKDSSLEITRFKGLGEISPKEFAAFIGP
EIRLTPVTITSLESISSILQFYMGKNTKERKQFIMDNLITDF
>Mature_601_residues
AAYTEASILSLASLDHIRLRAGMYIGRLGNGSQKEDGIYTLFKEVVDNGIDEFIMGHGKSLKISASDKQISIQDQGRGIP
LGKLIDCVSKINTGAKYTQDVFHFSVGLNGVGLKAVNALSEIFSVRSVRKKKYHLATFHRGVLQESKQGSTKDPDGTFVS
FTPDPSIFPEFTFNHDFLKDKIRQYTYLHSGLEIRFNDEVFISHNGLKDLFDAEITEPPLYSPLFFQNEDLTFIFSHLEG
NTERYFSFVNGQETLDGGTHLTAFKEAIVKGVNEFFGKTFVSNDIREGIVGCIAIKIASPIFESQTKNKLGNTQIRSSLI
KDVKEAIVQALRKDKVAPELLLEKIKFNEKTRKNIQFIKQDLKSKQKKVHYKIPKLRDCKFHYNDRSLYGEASSIFLTEG
ESASASILASRNPLTQAVFSLRGKPMNVFSLEETKMYKNDELFYLATALGITQNEIQHLRYNKVILATDADVDGMHIRNL
LITFFLKTLLPLVENNHLFILETPLFKVRNKTTTLYYYSEQEKMQALQQFGKKDSSLEITRFKGLGEISPKEFAAFIGPE
IRLTPVTITSLESISSILQFYMGKNTKERKQFIMDNLITDF

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=614, Percent_Identity=25.5700325732899, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI19913406, Length=610, Percent_Identity=23.9344262295082, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI48994957, Length=569, Percent_Identity=31.6344463971881, Blast_Score=251, Evalue=8e-68,
Organism=Escherichia coli, GI1789408, Length=637, Percent_Identity=28.2574568288854, Blast_Score=226, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI212645845, Length=330, Percent_Identity=28.4848484848485, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535065, Length=621, Percent_Identity=23.9935587761675, Blast_Score=82, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6324241, Length=626, Percent_Identity=24.1214057507987, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI17136538, Length=618, Percent_Identity=25.2427184466019, Blast_Score=104, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.3

Molecular weight: Translated: 68259; Mature: 68128

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAYTEASILSLASLDHIRLRAGMYIGRLGNGSQKEDGIYTLFKEVVDNGIDEFIMGHGK
CCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCC
SLKISASDKQISIQDQGRGIPLGKLIDCVSKINTGAKYTQDVFHFSVGLNGVGLKAVNAL
EEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHH
SEIFSVRSVRKKKYHLATFHRGVLQESKQGSTKDPDGTFVSFTPDPSIFPEFTFNHDFLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHH
DKIRQYTYLHSGLEIRFNDEVFISHNGLKDLFDAEITEPPLYSPLFFQNEDLTFIFSHLE
HHHHHHHHHHCCCEEEECCEEEEECCCHHHHHHCCCCCCCCCCCCEECCCCCEEEEECCC
GNTERYFSFVNGQETLDGGTHLTAFKEAIVKGVNEFFGKTFVSNDIREGIVGCIAIKIAS
CCHHHHEEECCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIFESQTKNKLGNTQIRSSLIKDVKEAIVQALRKDKVAPELLLEKIKFNEKTRKNIQFIK
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHH
QDLKSKQKKVHYKIPKLRDCKFHYNDRSLYGEASSIFLTEGESASASILASRNPLTQAVF
HHHHHHHHHEEECCCCCCCCEEEECCCCCCCCCCEEEEECCCCCCEEEEECCCHHHHHHH
SLRGKPMNVFSLEETKMYKNDELFYLATALGITQNEIQHLRYNKVILATDADVDGMHIRN
HHCCCCCEEEECHHHHHCCCCCEEEEEEHHCCCHHHHHHHHHCEEEEEECCCCCCHHHHH
LLITFFLKTLLPLVENNHLFILETPLFKVRNKTTTLYYYSEQEKMQALQQFGKKDSSLEI
HHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCEEEEEEECCHHHHHHHHHHCCCCCCEEE
TRFKGLGEISPKEFAAFIGPEIRLTPVTITSLESISSILQFYMGKNTKERKQFIMDNLIT
EEECCCCCCCHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
DF
CC
>Mature Secondary Structure 
AAYTEASILSLASLDHIRLRAGMYIGRLGNGSQKEDGIYTLFKEVVDNGIDEFIMGHGK
CCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCC
SLKISASDKQISIQDQGRGIPLGKLIDCVSKINTGAKYTQDVFHFSVGLNGVGLKAVNAL
EEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHH
SEIFSVRSVRKKKYHLATFHRGVLQESKQGSTKDPDGTFVSFTPDPSIFPEFTFNHDFLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHH
DKIRQYTYLHSGLEIRFNDEVFISHNGLKDLFDAEITEPPLYSPLFFQNEDLTFIFSHLE
HHHHHHHHHHCCCEEEECCEEEEECCCHHHHHHCCCCCCCCCCCCEECCCCCEEEEECCC
GNTERYFSFVNGQETLDGGTHLTAFKEAIVKGVNEFFGKTFVSNDIREGIVGCIAIKIAS
CCHHHHEEECCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIFESQTKNKLGNTQIRSSLIKDVKEAIVQALRKDKVAPELLLEKIKFNEKTRKNIQFIK
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHH
QDLKSKQKKVHYKIPKLRDCKFHYNDRSLYGEASSIFLTEGESASASILASRNPLTQAVF
HHHHHHHHHEEECCCCCCCCEEEECCCCCCCCCCEEEEECCCCCCEEEEECCCHHHHHHH
SLRGKPMNVFSLEETKMYKNDELFYLATALGITQNEIQHLRYNKVILATDADVDGMHIRN
HHCCCCCEEEECHHHHHCCCCCEEEEEEHHCCCHHHHHHHHHCEEEEEECCCCCCHHHHH
LLITFFLKTLLPLVENNHLFILETPLFKVRNKTTTLYYYSEQEKMQALQQFGKKDSSLEI
HHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCEEEEEEECCHHHHHHHHHHCCCCCCEEE
TRFKGLGEISPKEFAAFIGPEIRLTPVTITSLESISSILQFYMGKNTKERKQFIMDNLIT
EEECCCCCCCHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
DF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerization [C]

Inhibitor: CJ-12, 371; CJ-12, 372; Coumermycin; Doxorubicin; Sitafloxacin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685; 7812434 [H]