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The map label for this gene is gyrA_2

Identifier: 15836248

GI number: 15836248

Start: 802790

End: 804226

Strand: Direct

Name: gyrA_2

Synonym: CPj0716

Alternate gene names: 15836248

Gene position: 802790-804226 (Clockwise)

Preceding gene: 15836247

Following gene: 15836249

Centisome position: 65.45

GC content: 40.99

Gene sequence:

>1437_bases
ATGCGTGACGTTTCAGAGCTTTTTCGAACACATTTTATGCATTACGCGTCTTACGTAATTTTAGAGAGAGCGATTCCTCA
TATTCTTGATGGCTTAAAACCGGTGCAGCGTCGACTTCTATGGACTTTATTCCTTATGGACGACGGGAAAATGCATAAAG
TTGCCAATATTGCAGGAAGAACTATGGCTCTCCATCCCCATGGCGATGCCCCTATTGTTGAAGCTCTTGTTGTCTTAGCA
AATAAAGGCTACCTCATCGACACGCAAGGAAACTTCGGAAATCCCCTTACGGGAGATCCTCACGCTGCTGCCCGTTATAT
AGAAGCACGACTCAGTCCTTTAGCTCGAGAAACGCTCTTTAATACCGACTTGATAGCTTTTCATGACTCTTATGATGGAA
GAGAAAAAGAACCTGATATTTTACCTGCAAAGCTCCCCGTGCTTTTACTTCATGGTGTGGACGGGATTGCTGTGGGGATG
ACCACGAAAATTTTCCCTCACAATTTTGCAGAACTTTTGAAAGCGCAAATTGCAATTTTAAATGATAAAAAATTCACTGT
GTTTCCTGACTTTCCTTCGGGAGGATTGATGGATCCCTCGGAGTATCAAGATGGATTGGGATCGATTACACTGCGTGCAT
CTATAGACATTATTAATGATAAAACGCTTGTAGTGAAACAAATTTGTCCTCAATCTACGACTGAGACTTTGATCCGTTCT
ATAGAGAACGCAGCAAAACGTGGCACAATTAAAATCGATACCATCCAAGACTTCTCTACAGATGTCCCTCACATTGAAAT
TAAGCTGCCAAAAGGCTCTCGAGCCAAAGAGATGCTTCCCTTGTTATTCGAGCATACTGAATGCCAGGTGATTCTCTATT
CTAAGCCCACAGTCATTTACGAGAATAAGCCTGTAGAATGTTCGATATCCGAGATTCTCAAACTGCATACTACAGCTCTA
CAGGGGTATCTTGAAAAAGAACTTTTGTTGCTCCAAGAACAACTTACTTTGGACCATTATCATAAAACCTTAGAATACAT
CTTTATTAAACATAAGCTCTATGATTCTGTCCGAGAAGTCCTAGCCATAAACAAGAAAATTTCTGCTGATGACCTACATC
AAGCAGTGCTCCATGCTCTGGAGCCCTGGCTTCATGAGCTTGCAACTCCCGTTACAAAACAAGACACCTCTCAACTTGCT
TCACTAACGATTAAGAAAATCCTTTGCTTTAATGAAGAGGCATGCACTAAGGAACTGCTAGCCATAGAAAAAAAACAAGC
AGCGATACAAAAAGATCTTGGAAGATTAAAAGAAGTCACCGTCAAGTACCTCAAAGGACTTTTAGAACGCCATGGACACT
TAGGAGAGAGAAAAACACAGATCACAAACTTTAAGACGGCAAAGACATCTATCTTGAAACAACAAACCTTAATTTAA

Upstream 100 bases:

>100_bases
TTTAGAGAGCATTTCTTCGATCTTACAATTCTATATGGGGAAAAATACAAAAGAGAGAAAACAATTTATTATGGATAACC
TTATTACTGATTTTTAATTT

Downstream 100 bases:

>100_bases
AAAACTAAGTTTATCTAAAAAACTTCTGATTAATAAGAGATGGTAAAATATTTTCTTTTTAAATTAGAATTAAATTTATA
GACACTATAACCATTGTAGT

Product: DNA topoisomerase IV subunit A

Products: NA

Alternate protein names: Topoisomerase IV subunit A [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MRDVSELFRTHFMHYASYVILERAIPHILDGLKPVQRRLLWTLFLMDDGKMHKVANIAGRTMALHPHGDAPIVEALVVLA
NKGYLIDTQGNFGNPLTGDPHAAARYIEARLSPLARETLFNTDLIAFHDSYDGREKEPDILPAKLPVLLLHGVDGIAVGM
TTKIFPHNFAELLKAQIAILNDKKFTVFPDFPSGGLMDPSEYQDGLGSITLRASIDIINDKTLVVKQICPQSTTETLIRS
IENAAKRGTIKIDTIQDFSTDVPHIEIKLPKGSRAKEMLPLLFEHTECQVILYSKPTVIYENKPVECSISEILKLHTTAL
QGYLEKELLLLQEQLTLDHYHKTLEYIFIKHKLYDSVREVLAINKKISADDLHQAVLHALEPWLHELATPVTKQDTSQLA
SLTIKKILCFNEEACTKELLAIEKKQAAIQKDLGRLKEVTVKYLKGLLERHGHLGERKTQITNFKTAKTSILKQQTLI

Sequences:

>Translated_478_residues
MRDVSELFRTHFMHYASYVILERAIPHILDGLKPVQRRLLWTLFLMDDGKMHKVANIAGRTMALHPHGDAPIVEALVVLA
NKGYLIDTQGNFGNPLTGDPHAAARYIEARLSPLARETLFNTDLIAFHDSYDGREKEPDILPAKLPVLLLHGVDGIAVGM
TTKIFPHNFAELLKAQIAILNDKKFTVFPDFPSGGLMDPSEYQDGLGSITLRASIDIINDKTLVVKQICPQSTTETLIRS
IENAAKRGTIKIDTIQDFSTDVPHIEIKLPKGSRAKEMLPLLFEHTECQVILYSKPTVIYENKPVECSISEILKLHTTAL
QGYLEKELLLLQEQLTLDHYHKTLEYIFIKHKLYDSVREVLAINKKISADDLHQAVLHALEPWLHELATPVTKQDTSQLA
SLTIKKILCFNEEACTKELLAIEKKQAAIQKDLGRLKEVTVKYLKGLLERHGHLGERKTQITNFKTAKTSILKQQTLI
>Mature_478_residues
MRDVSELFRTHFMHYASYVILERAIPHILDGLKPVQRRLLWTLFLMDDGKMHKVANIAGRTMALHPHGDAPIVEALVVLA
NKGYLIDTQGNFGNPLTGDPHAAARYIEARLSPLARETLFNTDLIAFHDSYDGREKEPDILPAKLPVLLLHGVDGIAVGM
TTKIFPHNFAELLKAQIAILNDKKFTVFPDFPSGGLMDPSEYQDGLGSITLRASIDIINDKTLVVKQICPQSTTETLIRS
IENAAKRGTIKIDTIQDFSTDVPHIEIKLPKGSRAKEMLPLLFEHTECQVILYSKPTVIYENKPVECSISEILKLHTTAL
QGYLEKELLLLQEQLTLDHYHKTLEYIFIKHKLYDSVREVLAINKKISADDLHQAVLHALEPWLHELATPVTKQDTSQLA
SLTIKKILCFNEEACTKELLAIEKKQAAIQKDLGRLKEVTVKYLKGLLERHGHLGERKTQITNFKTAKTSILKQQTLI

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=263, Percent_Identity=27.3764258555133, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI19913408, Length=165, Percent_Identity=35.1515151515151, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1788562, Length=341, Percent_Identity=31.3782991202346, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1789396, Length=294, Percent_Identity=34.0136054421769, Blast_Score=146, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17535065, Length=166, Percent_Identity=34.9397590361446, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI212645845, Length=176, Percent_Identity=33.5227272727273, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI115534553, Length=159, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=231, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17136538, Length=256, Percent_Identity=28.515625, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013760 [H]

Pfam domain/function: PF00521 DNA_topoisoIV [H]

EC number: 5.99.1.3

Molecular weight: Translated: 53899; Mature: 53899

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDVSELFRTHFMHYASYVILERAIPHILDGLKPVQRRLLWTLFLMDDGKMHKVANIAGR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHEEECCCCHHHHHHHCCC
TMALHPHGDAPIVEALVVLANKGYLIDTQGNFGNPLTGDPHAAARYIEARLSPLARETLF
EEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
NTDLIAFHDSYDGREKEPDILPAKLPVLLLHGVDGIAVGMTTKIFPHNFAELLKAQIAIL
CCCEEEEECCCCCCCCCCCCCCCHHHHHEEECCCCEEECCEEECCCHHHHHHHHHHHHEE
NDKKFTVFPDFPSGGLMDPSEYQDGLGSITLRASIDIINDKTLVVKQICPQSTTETLIRS
CCCEEEEECCCCCCCCCCHHHHHCCCCCEEEEEEEEEECCCEEHHHHHCCCCHHHHHHHH
IENAAKRGTIKIDTIQDFSTDVPHIEIKLPKGSRAKEMLPLLFEHTECQVILYSKPTVIY
HHHHHHCCCEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCEEEE
ENKPVECSISEILKLHTTALQGYLEKELLLLQEQLTLDHYHKTLEYIFIKHKLYDSVREV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAINKKISADDLHQAVLHALEPWLHELATPVTKQDTSQLASLTIKKILCFNEEACTKELL
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AIEKKQAAIQKDLGRLKEVTVKYLKGLLERHGHLGERKTQITNFKTAKTSILKQQTLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRDVSELFRTHFMHYASYVILERAIPHILDGLKPVQRRLLWTLFLMDDGKMHKVANIAGR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHEEECCCCHHHHHHHCCC
TMALHPHGDAPIVEALVVLANKGYLIDTQGNFGNPLTGDPHAAARYIEARLSPLARETLF
EEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
NTDLIAFHDSYDGREKEPDILPAKLPVLLLHGVDGIAVGMTTKIFPHNFAELLKAQIAIL
CCCEEEEECCCCCCCCCCCCCCCHHHHHEEECCCCEEECCEEECCCHHHHHHHHHHHHEE
NDKKFTVFPDFPSGGLMDPSEYQDGLGSITLRASIDIINDKTLVVKQICPQSTTETLIRS
CCCEEEEECCCCCCCCCCHHHHHCCCCCEEEEEEEEEECCCEEHHHHHCCCCHHHHHHHH
IENAAKRGTIKIDTIQDFSTDVPHIEIKLPKGSRAKEMLPLLFEHTECQVILYSKPTVIY
HHHHHHCCCEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCEEEE
ENKPVECSISEILKLHTTALQGYLEKELLLLQEQLTLDHYHKTLEYIFIKHKLYDSVREV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAINKKISADDLHQAVLHALEPWLHELATPVTKQDTSQLASLTIKKILCFNEEACTKELL
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AIEKKQAAIQKDLGRLKEVTVKYLKGLLERHGHLGERKTQITNFKTAKTSILKQQTLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerization [C]

Inhibitor: CJ-12, 371; CJ-12, 372; Coumermycin; Doxorubicin; Sitafloxacin [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]